Ceratocystis polonica partitivirus
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6X9T6|Q6X9T6_9VIRU Putative RNA-dependent RNA polymerase OS=Ceratocystis polonica partitivirus OX=235434 GN=RDRP PE=4 SV=1
MM1 pKa = 7.15 SHH3 pKa = 7.84 DD4 pKa = 3.89 ARR6 pKa = 11.84 DD7 pKa = 3.75 LQSEE11 pKa = 4.18 ARR13 pKa = 11.84 QEE15 pKa = 3.93 VRR17 pKa = 11.84 PSGLQPDD24 pKa = 3.99 AHH26 pKa = 6.53 SHH28 pKa = 5.66 EE29 pKa = 5.37 PIGLNNSIARR39 pKa = 11.84 TAPSSSAGPGLTAPHH54 pKa = 6.94 AGTRR58 pKa = 11.84 SLGLPCKK65 pKa = 10.65 GEE67 pKa = 4.03 ASHH70 pKa = 7.34 SSEE73 pKa = 4.0 ASSLARR79 pKa = 11.84 DD80 pKa = 3.02 IDD82 pKa = 3.69 LGRR85 pKa = 11.84 NCSRR89 pKa = 11.84 SSGEE93 pKa = 4.01 RR94 pKa = 11.84 PFLIQVVPDD103 pKa = 3.55 SRR105 pKa = 11.84 FACYY109 pKa = 10.61 VFTEE113 pKa = 4.42 YY114 pKa = 10.93 VQQNYY119 pKa = 10.17 SKK121 pKa = 10.8 FDD123 pKa = 3.62 VEE125 pKa = 4.47 SSSMVSPATVVGYY138 pKa = 10.76 LMYY141 pKa = 10.3 CLHH144 pKa = 6.93 AFIFLTDD151 pKa = 4.0 VYY153 pKa = 11.09 SSSTMSAYY161 pKa = 10.32 AEE163 pKa = 4.73 EE164 pKa = 4.81 IDD166 pKa = 4.33 ASHH169 pKa = 7.23 IMRR172 pKa = 11.84 KK173 pKa = 10.11 LIDD176 pKa = 3.6 IFSNCLVPDD185 pKa = 3.71 IVFSVLDD192 pKa = 3.71 ALHH195 pKa = 6.04 PHH197 pKa = 6.57 KK198 pKa = 10.79 LDD200 pKa = 3.11 VRR202 pKa = 11.84 TTVSLFPSYY211 pKa = 11.16 GSVLFEE217 pKa = 3.96 YY218 pKa = 10.08 DD219 pKa = 3.67 APRR222 pKa = 11.84 LIPPSIFLLAHH233 pKa = 5.89 NQLLTQTKK241 pKa = 9.39 VPNSYY246 pKa = 10.65 KK247 pKa = 10.14 SWLSQTCVTYY257 pKa = 8.92 NTEE260 pKa = 4.21 DD261 pKa = 3.57 YY262 pKa = 10.77 RR263 pKa = 11.84 VGNIIGGIYY272 pKa = 7.73 QTISGSTTEE281 pKa = 4.06 TFQYY285 pKa = 8.85 KK286 pKa = 8.21 TWLLRR291 pKa = 11.84 SLSRR295 pKa = 11.84 LADD298 pKa = 3.5 SATHH302 pKa = 5.62 RR303 pKa = 11.84 THH305 pKa = 7.63 LKK307 pKa = 9.87 RR308 pKa = 11.84 NAVTEE313 pKa = 4.1 IQFDD317 pKa = 3.99 PPSFTDD323 pKa = 4.01 ADD325 pKa = 4.26 FNPYY329 pKa = 9.58 TYY331 pKa = 11.5 LLMLSPSSRR340 pKa = 11.84 TTTTSFLTALSSSCQKK356 pKa = 10.49 YY357 pKa = 10.81 LNATRR362 pKa = 11.84 SLSSVLATRR371 pKa = 11.84 SGSITRR377 pKa = 11.84 HH378 pKa = 5.44 MIFDD382 pKa = 4.25 LTAPTANYY390 pKa = 7.58 STLIDD395 pKa = 4.09 LDD397 pKa = 3.85 EE398 pKa = 5.33 SSVLKK403 pKa = 10.41 PGNFANFCKK412 pKa = 10.4 AVDD415 pKa = 4.36 FCPPQISNPPKK426 pKa = 10.52 KK427 pKa = 9.55 LTLPYY432 pKa = 9.57 PPGVEE437 pKa = 4.03 AHH439 pKa = 6.7 PSLYY443 pKa = 10.63 LVTEE447 pKa = 4.48 KK448 pKa = 10.75 EE449 pKa = 4.35 SEE451 pKa = 4.41 SPLKK455 pKa = 9.99 PLRR458 pKa = 11.84 PSYY461 pKa = 9.96 EE462 pKa = 3.92 DD463 pKa = 3.29 HH464 pKa = 7.77 VEE466 pKa = 4.04 GQILLFDD473 pKa = 5.31 PYY475 pKa = 11.24 DD476 pKa = 4.51 DD477 pKa = 4.37 EE478 pKa = 5.77 PSAHH482 pKa = 6.81 FSTLISGKK490 pKa = 10.35 LIEE493 pKa = 4.96 NGNVDD498 pKa = 4.78 GITIFLPSPSSSISAVNSRR517 pKa = 11.84 NLQGAIPLCRR527 pKa = 11.84 IQATFAGFPFQQYY540 pKa = 8.92 PRR542 pKa = 11.84 SHH544 pKa = 6.49 APSSRR549 pKa = 11.84 MFTMSILYY557 pKa = 9.8 DD558 pKa = 3.71 AANIWLPYY566 pKa = 8.77 FNRR569 pKa = 11.84 VMYY572 pKa = 10.23 SALPILPYY580 pKa = 9.08 TANRR584 pKa = 11.84 NADD587 pKa = 3.53 GLVPSTNLVVTSSKK601 pKa = 9.71 TVSTPSFSPDD611 pKa = 2.71 VSLWSSYY618 pKa = 10.07 RR619 pKa = 11.84 YY620 pKa = 9.66 QPTNEE625 pKa = 4.22 RR626 pKa = 11.84 PTPQNVYY633 pKa = 9.49 MYY635 pKa = 8.81 ATLEE639 pKa = 4.09 PFFGSRR645 pKa = 11.84 SSYY648 pKa = 9.03 LQSYY652 pKa = 7.65 QLQVLLPISS661 pKa = 3.62
Molecular weight: 73.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.714
IPC2_protein 5.804
IPC_protein 5.893
Toseland 6.3
ProMoST 6.262
Dawson 6.198
Bjellqvist 6.186
Wikipedia 6.211
Rodwell 6.186
Grimsley 6.453
Solomon 6.198
Lehninger 6.186
Nozaki 6.478
DTASelect 6.664
Thurlkill 6.693
EMBOSS 6.649
Sillero 6.576
Patrickios 3.35
IPC_peptide 6.211
IPC2_peptide 6.561
IPC2.peptide.svr19 6.479
Protein with the highest isoelectric point:
>tr|Q6X9T6|Q6X9T6_9VIRU Putative RNA-dependent RNA polymerase OS=Ceratocystis polonica partitivirus OX=235434 GN=RDRP PE=4 SV=1
MM1 pKa = 7.9 PSFSANPTYY10 pKa = 9.53 QTLIDD15 pKa = 5.93 DD16 pKa = 4.15 IDD18 pKa = 4.71 FDD20 pKa = 4.55 VPIAHH25 pKa = 7.36 PFSVINTDD33 pKa = 3.72 LQPVDD38 pKa = 4.91 DD39 pKa = 5.15 EE40 pKa = 4.72 EE41 pKa = 4.7 TVHH44 pKa = 7.38 DD45 pKa = 5.04 LGSKK49 pKa = 10.41 DD50 pKa = 3.34 FEE52 pKa = 4.57 FYY54 pKa = 10.89 KK55 pKa = 10.79 VVSDD59 pKa = 3.79 NLPPTRR65 pKa = 11.84 APSIGIEE72 pKa = 3.95 SLPNIRR78 pKa = 11.84 YY79 pKa = 8.9 HH80 pKa = 5.68 NHH82 pKa = 6.0 SNDD85 pKa = 2.53 HH86 pKa = 7.1 RR87 pKa = 11.84 YY88 pKa = 9.66 RR89 pKa = 11.84 DD90 pKa = 3.88 QPPTGPPPMRR100 pKa = 11.84 GVRR103 pKa = 11.84 KK104 pKa = 9.91 IINDD108 pKa = 3.62 SFPQYY113 pKa = 10.94 LPYY116 pKa = 10.56 LKK118 pKa = 9.89 EE119 pKa = 3.33 WCRR122 pKa = 11.84 PKK124 pKa = 10.42 TSSDD128 pKa = 3.95 AIFEE132 pKa = 4.84 DD133 pKa = 5.28 FNQPQIPSIPLSYY146 pKa = 10.39 NRR148 pKa = 11.84 KK149 pKa = 8.37 QRR151 pKa = 11.84 ILNLVNHH158 pKa = 6.29 FMGVKK163 pKa = 9.81 PYY165 pKa = 10.58 DD166 pKa = 3.31 IVHH169 pKa = 6.32 FCDD172 pKa = 3.01 TRR174 pKa = 11.84 FYY176 pKa = 10.71 PWDD179 pKa = 3.66 LSKK182 pKa = 11.18 KK183 pKa = 9.61 ADD185 pKa = 3.61 YY186 pKa = 10.16 FHH188 pKa = 6.67 NHH190 pKa = 3.66 SNARR194 pKa = 11.84 KK195 pKa = 8.35 RR196 pKa = 11.84 HH197 pKa = 5.02 AQTSHH202 pKa = 6.64 ASTATGPTKK211 pKa = 10.57 KK212 pKa = 10.02 SWFINAHH219 pKa = 6.13 LFHH222 pKa = 7.45 DD223 pKa = 4.68 RR224 pKa = 11.84 STVHH228 pKa = 6.61 NIKK231 pKa = 10.7 LYY233 pKa = 9.86 GLPFKK238 pKa = 9.99 PHH240 pKa = 6.52 SYY242 pKa = 9.37 EE243 pKa = 3.77 ARR245 pKa = 11.84 NKK247 pKa = 10.08 ILLEE251 pKa = 3.93 LWFKK255 pKa = 11.05 KK256 pKa = 10.31 IPTEE260 pKa = 3.77 LLVRR264 pKa = 11.84 SHH266 pKa = 7.07 ISNPKK271 pKa = 7.83 KK272 pKa = 10.73 LKK274 pKa = 9.98 VRR276 pKa = 11.84 PVNNAPMIFLRR287 pKa = 11.84 IEE289 pKa = 3.95 CMLFYY294 pKa = 10.57 PLLAQLRR301 pKa = 11.84 KK302 pKa = 7.74 QQCSIMYY309 pKa = 9.82 GLEE312 pKa = 4.19 TIRR315 pKa = 11.84 GGMMEE320 pKa = 4.35 IEE322 pKa = 4.23 SLATRR327 pKa = 11.84 FSNFMMIDD335 pKa = 3.05 WSKK338 pKa = 11.11 FDD340 pKa = 3.26 QTVPFTLVDD349 pKa = 3.2 MFYY352 pKa = 10.9 QDD354 pKa = 4.79 WIPTLILVDD363 pKa = 3.38 SGYY366 pKa = 11.66 AKK368 pKa = 9.51 IHH370 pKa = 6.27 NYY372 pKa = 9.35 NDD374 pKa = 3.68 HH375 pKa = 5.51 VHH377 pKa = 6.44 SFAAQARR384 pKa = 11.84 KK385 pKa = 10.07 LGVHH389 pKa = 6.7 GDD391 pKa = 3.75 SNLNEE396 pKa = 4.84 APPEE400 pKa = 3.94 AAVFANKK407 pKa = 9.76 VEE409 pKa = 4.08 NLLKK413 pKa = 10.72 FINTWFKK420 pKa = 10.55 EE421 pKa = 4.21 MVYY424 pKa = 8.81 ITPDD428 pKa = 2.69 GFAYY432 pKa = 10.53 SRR434 pKa = 11.84 TFAGVPSGILCTQLIDD450 pKa = 3.71 SFVNLVVLIDD460 pKa = 3.59 SLFEE464 pKa = 4.18 FGFHH468 pKa = 6.82 EE469 pKa = 4.67 SDD471 pKa = 3.13 IKK473 pKa = 11.04 SALILLMGDD482 pKa = 3.8 DD483 pKa = 4.13 NVVFAPDD490 pKa = 4.16 KK491 pKa = 10.85 LSQLHH496 pKa = 6.28 SFFKK500 pKa = 10.61 FLPDD504 pKa = 3.42 YY505 pKa = 10.98 AKK507 pKa = 10.6 KK508 pKa = 9.68 RR509 pKa = 11.84 WNMKK513 pKa = 9.67 VNVDD517 pKa = 3.29 KK518 pKa = 11.43 SIFTTLRR525 pKa = 11.84 RR526 pKa = 11.84 KK527 pKa = 9.82 IEE529 pKa = 3.67 ILGYY533 pKa = 7.61 TNNYY537 pKa = 8.42 GMPVRR542 pKa = 11.84 SLSKK546 pKa = 10.81 LIGQLAYY553 pKa = 9.75 PEE555 pKa = 4.29 RR556 pKa = 11.84 HH557 pKa = 6.15 VNDD560 pKa = 3.75 SDD562 pKa = 3.22 MCMRR566 pKa = 11.84 AIGFAWCAAASDD578 pKa = 4.22 STFHH582 pKa = 6.97 DD583 pKa = 4.67 FCRR586 pKa = 11.84 KK587 pKa = 8.92 VFIYY591 pKa = 10.29 YY592 pKa = 8.45 YY593 pKa = 11.0 ARR595 pKa = 11.84 VNVPIKK601 pKa = 10.89 DD602 pKa = 3.85 LVQSNASALPGMFFAYY618 pKa = 10.0 RR619 pKa = 11.84 DD620 pKa = 3.61 VHH622 pKa = 5.18 QHH624 pKa = 5.23 IKK626 pKa = 10.48 LDD628 pKa = 3.63 HH629 pKa = 6.35 FPSIEE634 pKa = 3.96 EE635 pKa = 3.88 VRR637 pKa = 11.84 QVLSKK642 pKa = 10.21 HH643 pKa = 5.82 HH644 pKa = 6.94 GYY646 pKa = 8.26 LTEE649 pKa = 4.23 EE650 pKa = 4.55 PLWKK654 pKa = 10.22 YY655 pKa = 11.4 DD656 pKa = 3.65 FFLHH660 pKa = 6.57 PKK662 pKa = 8.8 PP663 pKa = 4.15
Molecular weight: 76.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.322
IPC2_protein 7.673
IPC_protein 7.556
Toseland 7.512
ProMoST 8.141
Dawson 8.287
Bjellqvist 8.507
Wikipedia 8.244
Rodwell 8.317
Grimsley 7.629
Solomon 8.361
Lehninger 8.361
Nozaki 8.609
DTASelect 8.331
Thurlkill 8.39
EMBOSS 8.492
Sillero 8.653
Patrickios 4.279
IPC_peptide 8.346
IPC2_peptide 7.454
IPC2.peptide.svr19 7.579
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1324
661
663
662.0
75.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.118 ± 0.4
1.36 ± 0.114
5.665 ± 0.724
3.776 ± 0.004
5.891 ± 0.892
3.776 ± 0.117
3.55 ± 0.726
5.816 ± 0.611
4.456 ± 1.399
9.366 ± 0.572
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.19 ± 0.391
4.758 ± 0.5
7.477 ± 0.402
3.323 ± 0.228
4.834 ± 0.005
10.423 ± 2.483
5.816 ± 1.186
5.665 ± 0.162
1.057 ± 0.336
4.683 ± 0.23
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here