Rhynchosia golden mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q20CK1|Q20CK1_9GEMI Movement protein BC1 OS=Rhynchosia golden mosaic virus OX=117198 GN=BC1 PE=3 SV=1
MM1 pKa = 7.61GNLIYY6 pKa = 10.28TCSYY10 pKa = 10.15SSKK13 pKa = 9.64EE14 pKa = 3.89NTSARR19 pKa = 11.84ITDD22 pKa = 3.23SSTWYY27 pKa = 8.11PQPGQHH33 pKa = 6.1ISIRR37 pKa = 11.84TYY39 pKa = 10.94RR40 pKa = 11.84EE41 pKa = 3.84LNPAPTSSPTSTRR54 pKa = 11.84TEE56 pKa = 3.87TQLNGEE62 pKa = 4.42SSKK65 pKa = 10.09STADD69 pKa = 3.32QLEE72 pKa = 4.52EE73 pKa = 4.01VSNLLMTHH81 pKa = 5.78TQRR84 pKa = 11.84HH85 pKa = 4.54

Molecular weight: 9.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2L8B6|Q2L8B6_9GEMI Capsid protein OS=Rhynchosia golden mosaic virus OX=117198 GN=AR1 PE=3 SV=1
MM1 pKa = 7.98PKK3 pKa = 9.65RR4 pKa = 11.84DD5 pKa = 3.82APWRR9 pKa = 11.84LMAGTSKK16 pKa = 10.61VSRR19 pKa = 11.84SANYY23 pKa = 10.32SPGAGTGPKK32 pKa = 9.92SNRR35 pKa = 11.84ASAWVNRR42 pKa = 11.84PMYY45 pKa = 9.63RR46 pKa = 11.84KK47 pKa = 8.62PRR49 pKa = 11.84IYY51 pKa = 10.75RR52 pKa = 11.84MYY54 pKa = 10.49RR55 pKa = 11.84SPDD58 pKa = 3.29VPKK61 pKa = 10.81GCEE64 pKa = 4.39GPCKK68 pKa = 10.05VQSFEE73 pKa = 4.03SRR75 pKa = 11.84HH76 pKa = 5.87DD77 pKa = 3.49VTHH80 pKa = 5.92VGKK83 pKa = 10.19VLCISDD89 pKa = 3.46ITRR92 pKa = 11.84GSGITHH98 pKa = 6.35RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.24ILGKK114 pKa = 9.73VWMDD118 pKa = 3.29EE119 pKa = 4.01NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMFDD155 pKa = 3.99NEE157 pKa = 4.11PSTATVKK164 pKa = 10.85NDD166 pKa = 2.82LRR168 pKa = 11.84DD169 pKa = 3.54RR170 pKa = 11.84FQVMHH175 pKa = 6.97KK176 pKa = 9.73FYY178 pKa = 11.33AKK180 pKa = 9.12VTGGQYY186 pKa = 11.1ASNEE190 pKa = 3.73QALVKK195 pKa = 10.5RR196 pKa = 11.84FWKK199 pKa = 9.34VNNYY203 pKa = 7.92VVYY206 pKa = 10.54NHH208 pKa = 6.03QEE210 pKa = 3.42AAKK213 pKa = 10.4YY214 pKa = 9.25EE215 pKa = 4.13NHH217 pKa = 6.46TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.77ATLKK240 pKa = 9.27IRR242 pKa = 11.84VYY244 pKa = 10.58FYY246 pKa = 11.3DD247 pKa = 4.39SIMNN251 pKa = 4.24

Molecular weight: 28.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1513

85

358

216.1

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.089 ± 0.51

1.785 ± 0.282

4.957 ± 0.437

4.032 ± 0.471

4.759 ± 0.472

5.221 ± 0.436

3.833 ± 0.296

5.486 ± 0.635

5.948 ± 0.411

6.543 ± 0.539

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.049 ± 0.527

6.081 ± 0.402

5.552 ± 0.565

5.089 ± 1.099

6.609 ± 0.657

9.319 ± 1.051

5.75 ± 0.614

6.477 ± 1.348

1.52 ± 0.176

3.9 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski