Amino acid dipepetide frequency for Anoxybacillus vitaminiphilus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.418AlaAla: 6.418 ± 0.121
0.589AlaCys: 0.589 ± 0.026
3.309AlaAsp: 3.309 ± 0.054
5.135AlaGlu: 5.135 ± 0.089
3.351AlaPhe: 3.351 ± 0.068
5.172AlaGly: 5.172 ± 0.088
1.424AlaHis: 1.424 ± 0.031
5.779AlaIle: 5.779 ± 0.082
5.674AlaLys: 5.674 ± 0.095
7.454AlaLeu: 7.454 ± 0.098
2.007AlaMet: 2.007 ± 0.048
2.915AlaAsn: 2.915 ± 0.06
2.097AlaPro: 2.097 ± 0.051
2.517AlaGln: 2.517 ± 0.056
2.907AlaArg: 2.907 ± 0.056
3.593AlaSer: 3.593 ± 0.066
3.322AlaThr: 3.322 ± 0.067
5.641AlaVal: 5.641 ± 0.096
0.576AlaTrp: 0.576 ± 0.023
2.497AlaTyr: 2.497 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.458CysAla: 0.458 ± 0.025
0.127CysCys: 0.127 ± 0.014
0.396CysAsp: 0.396 ± 0.021
0.52CysGlu: 0.52 ± 0.025
0.396CysPhe: 0.396 ± 0.023
0.717CysGly: 0.717 ± 0.03
0.22CysHis: 0.22 ± 0.015
0.523CysIle: 0.523 ± 0.025
0.438CysLys: 0.438 ± 0.027
0.732CysLeu: 0.732 ± 0.03
0.205CysMet: 0.205 ± 0.014
0.293CysAsn: 0.293 ± 0.016
0.394CysPro: 0.394 ± 0.021
0.264CysGln: 0.264 ± 0.019
0.359CysArg: 0.359 ± 0.023
0.492CysSer: 0.492 ± 0.021
0.419CysThr: 0.419 ± 0.02
0.449CysVal: 0.449 ± 0.023
0.068CysTrp: 0.068 ± 0.007
0.295CysTyr: 0.295 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.052AspAla: 3.052 ± 0.064
0.409AspCys: 0.409 ± 0.02
2.25AspAsp: 2.25 ± 0.049
4.632AspGlu: 4.632 ± 0.077
2.218AspPhe: 2.218 ± 0.053
3.161AspGly: 3.161 ± 0.064
0.939AspHis: 0.939 ± 0.029
4.1AspIle: 4.1 ± 0.075
2.779AspLys: 2.779 ± 0.054
4.59AspLeu: 4.59 ± 0.072
1.242AspMet: 1.242 ± 0.035
1.506AspAsn: 1.506 ± 0.042
1.948AspPro: 1.948 ± 0.046
1.403AspGln: 1.403 ± 0.043
2.08AspArg: 2.08 ± 0.049
2.077AspSer: 2.077 ± 0.046
2.029AspThr: 2.029 ± 0.049
3.789AspVal: 3.789 ± 0.068
0.617AspTrp: 0.617 ± 0.026
2.068AspTyr: 2.068 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.881GluAla: 5.881 ± 0.089
0.431GluCys: 0.431 ± 0.023
3.257GluAsp: 3.257 ± 0.065
7.619GluGlu: 7.619 ± 0.122
2.691GluPhe: 2.691 ± 0.057
4.16GluGly: 4.16 ± 0.063
1.819GluHis: 1.819 ± 0.047
6.134GluIle: 6.134 ± 0.085
7.458GluLys: 7.458 ± 0.106
7.374GluLeu: 7.374 ± 0.098
2.534GluMet: 2.534 ± 0.055
3.699GluAsn: 3.699 ± 0.059
2.113GluPro: 2.113 ± 0.046
4.313GluGln: 4.313 ± 0.084
4.383GluArg: 4.383 ± 0.077
2.915GluSer: 2.915 ± 0.06
4.025GluThr: 4.025 ± 0.072
5.17GluVal: 5.17 ± 0.077
0.879GluTrp: 0.879 ± 0.032
2.336GluTyr: 2.336 ± 0.052
0.0GluXaa: 0.0 ± 0.0
Phe
3.422PheAla: 3.422 ± 0.063
0.383PheCys: 0.383 ± 0.022
2.186PheAsp: 2.186 ± 0.049
2.808PheGlu: 2.808 ± 0.057
2.368PhePhe: 2.368 ± 0.061
3.179PheGly: 3.179 ± 0.057
1.096PheHis: 1.096 ± 0.037
3.919PheIle: 3.919 ± 0.075
2.237PheLys: 2.237 ± 0.051
4.664PheLeu: 4.664 ± 0.091
1.18PheMet: 1.18 ± 0.036
1.604PheAsn: 1.604 ± 0.04
1.694PhePro: 1.694 ± 0.044
1.713PheGln: 1.713 ± 0.042
1.761PheArg: 1.761 ± 0.042
3.044PheSer: 3.044 ± 0.059
2.508PheThr: 2.508 ± 0.055
3.274PheVal: 3.274 ± 0.069
0.488PheTrp: 0.488 ± 0.022
1.82PheTyr: 1.82 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.702GlyAla: 4.702 ± 0.084
0.63GlyCys: 0.63 ± 0.025
2.901GlyAsp: 2.901 ± 0.057
4.595GlyGlu: 4.595 ± 0.069
3.106GlyPhe: 3.106 ± 0.056
4.47GlyGly: 4.47 ± 0.093
1.34GlyHis: 1.34 ± 0.04
5.701GlyIle: 5.701 ± 0.084
5.312GlyLys: 5.312 ± 0.079
6.205GlyLeu: 6.205 ± 0.096
2.008GlyMet: 2.008 ± 0.044
2.547GlyAsn: 2.547 ± 0.055
1.668GlyPro: 1.668 ± 0.047
2.177GlyGln: 2.177 ± 0.051
2.79GlyArg: 2.79 ± 0.05
3.262GlySer: 3.262 ± 0.06
3.824GlyThr: 3.824 ± 0.076
4.904GlyVal: 4.904 ± 0.085
0.747GlyTrp: 0.747 ± 0.028
2.803GlyTyr: 2.803 ± 0.051
0.0GlyXaa: 0.0 ± 0.0
His
1.348HisAla: 1.348 ± 0.032
0.226HisCys: 0.226 ± 0.014
0.996HisAsp: 0.996 ± 0.032
1.525HisGlu: 1.525 ± 0.04
1.097HisPhe: 1.097 ± 0.036
1.454HisGly: 1.454 ± 0.041
0.794HisHis: 0.794 ± 0.031
1.656HisIle: 1.656 ± 0.041
1.121HisLys: 1.121 ± 0.04
2.424HisLeu: 2.424 ± 0.057
0.578HisMet: 0.578 ± 0.024
0.739HisAsn: 0.739 ± 0.026
1.257HisPro: 1.257 ± 0.035
0.908HisGln: 0.908 ± 0.028
1.041HisArg: 1.041 ± 0.034
1.238HisSer: 1.238 ± 0.043
0.987HisThr: 0.987 ± 0.03
1.595HisVal: 1.595 ± 0.035
0.235HisTrp: 0.235 ± 0.014
0.997HisTyr: 0.997 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
6.336IleAla: 6.336 ± 0.082
0.697IleCys: 0.697 ± 0.029
4.357IleAsp: 4.357 ± 0.072
6.247IleGlu: 6.247 ± 0.088
3.258IlePhe: 3.258 ± 0.068
5.846IleGly: 5.846 ± 0.097
1.918IleHis: 1.918 ± 0.044
5.673IleIle: 5.673 ± 0.085
4.594IleLys: 4.594 ± 0.069
6.6IleLeu: 6.6 ± 0.088
1.646IleMet: 1.646 ± 0.036
2.8IleAsn: 2.8 ± 0.055
3.443IlePro: 3.443 ± 0.063
3.025IleGln: 3.025 ± 0.062
3.549IleArg: 3.549 ± 0.064
4.655IleSer: 4.655 ± 0.071
4.095IleThr: 4.095 ± 0.068
6.026IleVal: 6.026 ± 0.084
0.64IleTrp: 0.64 ± 0.027
2.656IleTyr: 2.656 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.24LysAla: 5.24 ± 0.096
0.372LysCys: 0.372 ± 0.019
3.423LysAsp: 3.423 ± 0.063
7.588LysGlu: 7.588 ± 0.104
2.057LysPhe: 2.057 ± 0.041
4.526LysGly: 4.526 ± 0.071
1.468LysHis: 1.468 ± 0.039
5.061LysIle: 5.061 ± 0.075
6.247LysLys: 6.247 ± 0.097
6.063LysLeu: 6.063 ± 0.081
2.306LysMet: 2.306 ± 0.049
3.345LysAsn: 3.345 ± 0.066
2.295LysPro: 2.295 ± 0.048
3.895LysGln: 3.895 ± 0.073
3.926LysArg: 3.926 ± 0.067
2.988LysSer: 2.988 ± 0.058
3.836LysThr: 3.836 ± 0.062
4.882LysVal: 4.882 ± 0.084
0.886LysTrp: 0.886 ± 0.028
2.411LysTyr: 2.411 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
7.666LeuAla: 7.666 ± 0.096
0.716LeuCys: 0.716 ± 0.031
4.492LeuAsp: 4.492 ± 0.069
7.108LeuGlu: 7.108 ± 0.102
4.901LeuPhe: 4.901 ± 0.092
6.104LeuGly: 6.104 ± 0.089
2.118LeuHis: 2.118 ± 0.044
6.731LeuIle: 6.731 ± 0.1
7.079LeuLys: 7.079 ± 0.087
10.373LeuLeu: 10.373 ± 0.146
2.323LeuMet: 2.323 ± 0.052
3.902LeuAsn: 3.902 ± 0.067
4.041LeuPro: 4.041 ± 0.065
4.179LeuGln: 4.179 ± 0.079
4.122LeuArg: 4.122 ± 0.083
6.37LeuSer: 6.37 ± 0.086
5.383LeuThr: 5.383 ± 0.075
6.197LeuVal: 6.197 ± 0.08
0.808LeuTrp: 0.808 ± 0.029
3.258LeuTyr: 3.258 ± 0.07
0.0LeuXaa: 0.0 ± 0.0
Met
2.051MetAla: 2.051 ± 0.047
0.149MetCys: 0.149 ± 0.013
1.265MetAsp: 1.265 ± 0.037
2.022MetGlu: 2.022 ± 0.048
1.116MetPhe: 1.116 ± 0.039
1.567MetGly: 1.567 ± 0.04
0.43MetHis: 0.43 ± 0.019
2.3MetIle: 2.3 ± 0.051
2.719MetLys: 2.719 ± 0.062
2.57MetLeu: 2.57 ± 0.054
0.963MetMet: 0.963 ± 0.034
1.571MetAsn: 1.571 ± 0.039
0.99MetPro: 0.99 ± 0.035
0.919MetGln: 0.919 ± 0.027
1.169MetArg: 1.169 ± 0.036
1.567MetSer: 1.567 ± 0.039
1.521MetThr: 1.521 ± 0.035
1.637MetVal: 1.637 ± 0.042
0.183MetTrp: 0.183 ± 0.013
0.78MetTyr: 0.78 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.557AsnAla: 2.557 ± 0.055
0.321AsnCys: 0.321 ± 0.017
2.091AsnAsp: 2.091 ± 0.055
3.717AsnGlu: 3.717 ± 0.068
1.438AsnPhe: 1.438 ± 0.039
3.363AsnGly: 3.363 ± 0.069
1.048AsnHis: 1.048 ± 0.033
3.321AsnIle: 3.321 ± 0.062
2.855AsnLys: 2.855 ± 0.061
3.345AsnLeu: 3.345 ± 0.062
1.138AsnMet: 1.138 ± 0.033
1.685AsnAsn: 1.685 ± 0.052
2.066AsnPro: 2.066 ± 0.048
1.515AsnGln: 1.515 ± 0.044
2.043AsnArg: 2.043 ± 0.051
1.834AsnSer: 1.834 ± 0.046
1.773AsnThr: 1.773 ± 0.044
3.114AsnVal: 3.114 ± 0.063
0.456AsnTrp: 0.456 ± 0.021
1.413AsnTyr: 1.413 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
2.256ProAla: 2.256 ± 0.051
0.248ProCys: 0.248 ± 0.014
1.885ProAsp: 1.885 ± 0.044
2.918ProGlu: 2.918 ± 0.057
2.172ProPhe: 2.172 ± 0.054
2.23ProGly: 2.23 ± 0.061
0.936ProHis: 0.936 ± 0.028
2.896ProIle: 2.896 ± 0.055
2.475ProLys: 2.475 ± 0.047
3.768ProLeu: 3.768 ± 0.065
0.827ProMet: 0.827 ± 0.031
1.835ProAsn: 1.835 ± 0.046
1.104ProPro: 1.104 ± 0.037
1.131ProGln: 1.131 ± 0.039
1.247ProArg: 1.247 ± 0.034
2.212ProSer: 2.212 ± 0.049
1.902ProThr: 1.902 ± 0.048
2.808ProVal: 2.808 ± 0.054
0.362ProTrp: 0.362 ± 0.022
1.542ProTyr: 1.542 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.884GlnAla: 2.884 ± 0.055
0.26GlnCys: 0.26 ± 0.018
1.393GlnAsp: 1.393 ± 0.041
3.031GlnGlu: 3.031 ± 0.06
1.806GlnPhe: 1.806 ± 0.046
2.063GlnGly: 2.063 ± 0.05
0.926GlnHis: 0.926 ± 0.031
2.808GlnIle: 2.808 ± 0.053
3.068GlnLys: 3.068 ± 0.065
4.562GlnLeu: 4.562 ± 0.081
1.34GlnMet: 1.34 ± 0.035
1.5GlnAsn: 1.5 ± 0.041
1.343GlnPro: 1.343 ± 0.041
2.194GlnGln: 2.194 ± 0.062
1.752GlnArg: 1.752 ± 0.041
2.023GlnSer: 2.023 ± 0.046
2.192GlnThr: 2.192 ± 0.052
2.256GlnVal: 2.256 ± 0.059
0.501GlnTrp: 0.501 ± 0.025
1.535GlnTyr: 1.535 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
2.763ArgAla: 2.763 ± 0.061
0.328ArgCys: 0.328 ± 0.022
2.041ArgAsp: 2.041 ± 0.046
3.745ArgGlu: 3.745 ± 0.07
2.222ArgPhe: 2.222 ± 0.049
2.468ArgGly: 2.468 ± 0.047
0.917ArgHis: 0.917 ± 0.034
3.45ArgIle: 3.45 ± 0.063
3.653ArgLys: 3.653 ± 0.062
4.735ArgLeu: 4.735 ± 0.086
1.379ArgMet: 1.379 ± 0.041
1.926ArgAsn: 1.926 ± 0.048
1.503ArgPro: 1.503 ± 0.04
1.812ArgGln: 1.812 ± 0.044
2.115ArgArg: 2.115 ± 0.052
2.201ArgSer: 2.201 ± 0.054
2.203ArgThr: 2.203 ± 0.046
2.789ArgVal: 2.789 ± 0.054
0.453ArgTrp: 0.453 ± 0.023
1.826ArgTyr: 1.826 ± 0.044
0.0ArgXaa: 0.0 ± 0.0
Ser
3.454SerAla: 3.454 ± 0.066
0.439SerCys: 0.439 ± 0.023
2.333SerAsp: 2.333 ± 0.053
3.686SerGlu: 3.686 ± 0.07
3.079SerPhe: 3.079 ± 0.059
3.818SerGly: 3.818 ± 0.064
1.139SerHis: 1.139 ± 0.033
4.321SerIle: 4.321 ± 0.066
3.33SerLys: 3.33 ± 0.061
5.932SerLeu: 5.932 ± 0.089
1.484SerMet: 1.484 ± 0.036
1.978SerAsn: 1.978 ± 0.052
2.053SerPro: 2.053 ± 0.042
1.762SerGln: 1.762 ± 0.041
2.168SerArg: 2.168 ± 0.051
3.319SerSer: 3.319 ± 0.07
2.496SerThr: 2.496 ± 0.049
3.852SerVal: 3.852 ± 0.066
0.548SerTrp: 0.548 ± 0.02
2.036SerTyr: 2.036 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
3.919ThrAla: 3.919 ± 0.063
0.367ThrCys: 0.367 ± 0.021
2.383ThrAsp: 2.383 ± 0.053
3.477ThrGlu: 3.477 ± 0.065
2.585ThrPhe: 2.585 ± 0.055
3.871ThrGly: 3.871 ± 0.065
1.016ThrHis: 1.016 ± 0.034
4.423ThrIle: 4.423 ± 0.075
3.638ThrLys: 3.638 ± 0.063
4.971ThrLeu: 4.971 ± 0.069
1.307ThrMet: 1.307 ± 0.039
2.366ThrAsn: 2.366 ± 0.047
2.189ThrPro: 2.189 ± 0.047
1.252ThrGln: 1.252 ± 0.039
1.752ThrArg: 1.752 ± 0.046
2.696ThrSer: 2.696 ± 0.051
2.704ThrThr: 2.704 ± 0.056
4.2ThrVal: 4.2 ± 0.068
0.521ThrTrp: 0.521 ± 0.022
1.892ThrTyr: 1.892 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
5.133ValAla: 5.133 ± 0.094
0.659ValCys: 0.659 ± 0.028
3.452ValAsp: 3.452 ± 0.063
5.074ValGlu: 5.074 ± 0.089
3.072ValPhe: 3.072 ± 0.058
4.374ValGly: 4.374 ± 0.066
1.522ValHis: 1.522 ± 0.039
5.817ValIle: 5.817 ± 0.084
5.047ValLys: 5.047 ± 0.076
6.83ValLeu: 6.83 ± 0.098
1.789ValMet: 1.789 ± 0.046
2.978ValAsn: 2.978 ± 0.056
2.816ValPro: 2.816 ± 0.057
2.601ValGln: 2.601 ± 0.054
3.002ValArg: 3.002 ± 0.06
4.271ValSer: 4.271 ± 0.057
4.061ValThr: 4.061 ± 0.082
5.315ValVal: 5.315 ± 0.089
0.654ValTrp: 0.654 ± 0.028
2.494ValTyr: 2.494 ± 0.056
0.0ValXaa: 0.0 ± 0.0
Trp
0.622TrpAla: 0.622 ± 0.024
0.059TrpCys: 0.059 ± 0.008
0.448TrpAsp: 0.448 ± 0.022
0.703TrpGlu: 0.703 ± 0.027
0.535TrpPhe: 0.535 ± 0.025
0.624TrpGly: 0.624 ± 0.024
0.225TrpHis: 0.225 ± 0.014
0.777TrpIle: 0.777 ± 0.029
0.851TrpLys: 0.851 ± 0.03
1.206TrpLeu: 1.206 ± 0.035
0.392TrpMet: 0.392 ± 0.021
0.527TrpAsn: 0.527 ± 0.023
0.285TrpPro: 0.285 ± 0.019
0.381TrpGln: 0.381 ± 0.02
0.457TrpArg: 0.457 ± 0.024
0.514TrpSer: 0.514 ± 0.022
0.476TrpThr: 0.476 ± 0.023
0.585TrpVal: 0.585 ± 0.026
0.128TrpTrp: 0.128 ± 0.013
0.357TrpTyr: 0.357 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.213TyrAla: 2.213 ± 0.053
0.359TyrCys: 0.359 ± 0.021
2.029TyrAsp: 2.029 ± 0.058
3.042TyrGlu: 3.042 ± 0.054
1.882TyrPhe: 1.882 ± 0.045
2.525TyrGly: 2.525 ± 0.055
0.921TyrHis: 0.921 ± 0.029
2.673TyrIle: 2.673 ± 0.05
2.243TyrLys: 2.243 ± 0.052
3.473TyrLeu: 3.473 ± 0.061
0.836TyrMet: 0.836 ± 0.031
1.377TyrAsn: 1.377 ± 0.04
1.455TyrPro: 1.455 ± 0.039
1.475TyrGln: 1.475 ± 0.04
1.905TyrArg: 1.905 ± 0.046
1.958TyrSer: 1.958 ± 0.049
1.772TyrThr: 1.772 ± 0.04
2.478TyrVal: 2.478 ± 0.053
0.399TyrTrp: 0.399 ± 0.019
1.595TyrTyr: 1.595 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3513 proteins (988767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski