Apis mellifera associated microvirus 56

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTT3|A0A3S8UTT3_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 56 OX=2494787 PE=3 SV=1
MM1 pKa = 7.67EE2 pKa = 5.74KK3 pKa = 10.74GGLQASLFPKK13 pKa = 10.31HH14 pKa = 6.32LLNMDD19 pKa = 4.48FVTHH23 pKa = 4.97EE24 pKa = 4.26TEE26 pKa = 4.38EE27 pKa = 4.44EE28 pKa = 3.98FLSTFDD34 pKa = 4.09DD35 pKa = 3.49QMEE38 pKa = 3.96AVYY41 pKa = 10.57RR42 pKa = 11.84FFFVTPNTHH51 pKa = 6.45AVAIKK56 pKa = 10.69NLLEE60 pKa = 4.09QVWDD64 pKa = 3.86KK65 pKa = 11.08FPEE68 pKa = 4.14LRR70 pKa = 11.84PAIATVSNDD79 pKa = 2.73YY80 pKa = 9.47WRR82 pKa = 11.84QFTFSLDD89 pKa = 3.37FADD92 pKa = 4.28LHH94 pKa = 7.27IGQQVHH100 pKa = 6.0VLRR103 pKa = 11.84CLYY106 pKa = 10.5NCVISYY112 pKa = 10.83INTQNEE118 pKa = 4.4SASSQNFITPQQQ130 pKa = 3.26

Molecular weight: 15.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTR4|A0A3S8UTR4_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 56 OX=2494787 PE=4 SV=1
MM1 pKa = 7.4LTFKK5 pKa = 10.72NISYY9 pKa = 10.86GKK11 pKa = 7.26TQIQAYY17 pKa = 8.83HH18 pKa = 6.16RR19 pKa = 11.84HH20 pKa = 5.35APGWNPPVTKK30 pKa = 9.76PQQHH34 pKa = 5.09TKK36 pKa = 10.43ARR38 pKa = 11.84SAFGKK43 pKa = 10.19SAIALPSATNVLL55 pKa = 3.82

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1486

55

546

247.7

27.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.287 ± 1.087

0.74 ± 0.292

5.316 ± 0.581

5.585 ± 0.692

4.576 ± 1.023

6.393 ± 1.257

2.557 ± 0.589

5.653 ± 0.214

5.249 ± 1.168

7.402 ± 0.544

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.143

5.451 ± 0.772

5.249 ± 0.889

5.855 ± 0.987

5.989 ± 0.886

6.326 ± 0.243

6.326 ± 0.43

4.172 ± 0.428

1.884 ± 0.222

3.297 ± 0.539
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski