Streptomyces sp. BK239

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5360 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7XPQ3|A0A4Q7XPQ3_9ACTN Penicillin-insensitive transglycosylase OS=Streptomyces sp. BK239 OX=2512155 GN=EV567_0606 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84QTLTRR7 pKa = 11.84GMIAAAAATSLLSLCGGAAYY27 pKa = 10.25ADD29 pKa = 3.8SSADD33 pKa = 3.33GTATGSPGAVSGAGVQSPVTLPASLCGDD61 pKa = 3.81TAGLAAAFGQALGDD75 pKa = 4.13FCADD79 pKa = 3.41TSDD82 pKa = 3.65EE83 pKa = 4.39TSTSDD88 pKa = 4.8AADD91 pKa = 3.43TSDD94 pKa = 5.31AEE96 pKa = 4.39DD97 pKa = 3.52TADD100 pKa = 3.54EE101 pKa = 4.56AKK103 pKa = 8.21TTEE106 pKa = 4.45DD107 pKa = 3.73ASTSAPADD115 pKa = 3.32GAARR119 pKa = 11.84ADD121 pKa = 3.74EE122 pKa = 4.38ATSAGADD129 pKa = 3.55SDD131 pKa = 4.82SDD133 pKa = 3.95HH134 pKa = 7.39ADD136 pKa = 3.22PGYY139 pKa = 11.18GEE141 pKa = 4.83DD142 pKa = 4.11PGYY145 pKa = 11.18GNPPHH150 pKa = 7.12EE151 pKa = 5.36DD152 pKa = 3.66DD153 pKa = 3.99EE154 pKa = 5.45PGYY157 pKa = 10.98GDD159 pKa = 4.38PGQGGDD165 pKa = 4.06KK166 pKa = 10.79DD167 pKa = 3.72PGYY170 pKa = 10.66GGHH173 pKa = 7.11HH174 pKa = 7.31DD175 pKa = 4.28GDD177 pKa = 3.98PGYY180 pKa = 11.05GGDD183 pKa = 4.79DD184 pKa = 3.7GPGYY188 pKa = 11.03GDD190 pKa = 5.61DD191 pKa = 4.9DD192 pKa = 5.21DD193 pKa = 5.94SGYY196 pKa = 11.44GSDD199 pKa = 5.19DD200 pKa = 3.6GPGYY204 pKa = 11.13GDD206 pKa = 4.91DD207 pKa = 3.8DD208 pKa = 4.1SGYY211 pKa = 11.22GSDD214 pKa = 5.19DD215 pKa = 3.6GPGYY219 pKa = 11.07GDD221 pKa = 5.61DD222 pKa = 4.9DD223 pKa = 5.17DD224 pKa = 5.95SGYY227 pKa = 11.32GSTPPPPGGPVTPPEE242 pKa = 4.75GGHH245 pKa = 5.25TTPHH249 pKa = 7.12DD250 pKa = 4.41PPPPGDD256 pKa = 4.76DD257 pKa = 4.08DD258 pKa = 5.55HH259 pKa = 6.56GTPPGEE265 pKa = 4.47EE266 pKa = 4.01NNEE269 pKa = 3.91PRR271 pKa = 11.84PQLPEE276 pKa = 3.98TGSEE280 pKa = 4.21SLLAASGVSAALITAGALLYY300 pKa = 10.54RR301 pKa = 11.84RR302 pKa = 11.84GRR304 pKa = 11.84AASRR308 pKa = 11.84RR309 pKa = 3.88

Molecular weight: 30.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7XFA3|A0A4Q7XFA3_9ACTN Parallel beta helix pectate lyase-like protein OS=Streptomyces sp. BK239 OX=2512155 GN=EV567_2076 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5360

0

5360

1794248

29

3538

334.7

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.81 ± 0.049

0.773 ± 0.01

5.994 ± 0.026

5.787 ± 0.035

2.666 ± 0.02

9.524 ± 0.032

2.28 ± 0.018

2.959 ± 0.023

2.288 ± 0.025

10.169 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.012

1.732 ± 0.019

6.206 ± 0.032

2.766 ± 0.019

8.147 ± 0.04

5.046 ± 0.026

6.04 ± 0.033

8.571 ± 0.029

1.487 ± 0.014

2.055 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski