Amino acid dipepetide frequency for Micromonospora sp. KC723

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.774AlaAla: 22.774 ± 0.183
1.0AlaCys: 1.0 ± 0.021
8.756AlaAsp: 8.756 ± 0.078
8.231AlaGlu: 8.231 ± 0.1
3.2AlaPhe: 3.2 ± 0.044
14.78AlaGly: 14.78 ± 0.11
2.586AlaHis: 2.586 ± 0.034
3.649AlaIle: 3.649 ± 0.047
2.189AlaLys: 2.189 ± 0.04
14.632AlaLeu: 14.632 ± 0.126
2.355AlaMet: 2.355 ± 0.039
2.242AlaAsn: 2.242 ± 0.045
7.085AlaPro: 7.085 ± 0.083
3.465AlaGln: 3.465 ± 0.048
11.003AlaArg: 11.003 ± 0.095
5.529AlaSer: 5.529 ± 0.065
8.385AlaThr: 8.385 ± 0.077
13.242AlaVal: 13.242 ± 0.123
1.988AlaTrp: 1.988 ± 0.039
2.899AlaTyr: 2.899 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.032CysAla: 1.032 ± 0.026
0.101CysCys: 0.101 ± 0.009
0.473CysAsp: 0.473 ± 0.018
0.335CysGlu: 0.335 ± 0.013
0.221CysPhe: 0.221 ± 0.012
0.931CysGly: 0.931 ± 0.023
0.21CysHis: 0.21 ± 0.011
0.122CysIle: 0.122 ± 0.009
0.084CysLys: 0.084 ± 0.006
0.725CysLeu: 0.725 ± 0.019
0.092CysMet: 0.092 ± 0.007
0.143CysAsn: 0.143 ± 0.009
0.498CysPro: 0.498 ± 0.018
0.189CysGln: 0.189 ± 0.011
0.664CysArg: 0.664 ± 0.022
0.378CysSer: 0.378 ± 0.016
0.43CysThr: 0.43 ± 0.016
0.636CysVal: 0.636 ± 0.02
0.147CysTrp: 0.147 ± 0.01
0.151CysTyr: 0.151 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.49AspAla: 7.49 ± 0.069
0.417AspCys: 0.417 ± 0.018
3.721AspAsp: 3.721 ± 0.055
3.597AspGlu: 3.597 ± 0.057
1.472AspPhe: 1.472 ± 0.03
6.347AspGly: 6.347 ± 0.072
1.342AspHis: 1.342 ± 0.031
1.657AspIle: 1.657 ± 0.031
0.846AspLys: 0.846 ± 0.026
6.43AspLeu: 6.43 ± 0.077
0.691AspMet: 0.691 ± 0.02
1.0AspAsn: 1.0 ± 0.028
4.798AspPro: 4.798 ± 0.059
1.68AspGln: 1.68 ± 0.033
5.638AspArg: 5.638 ± 0.061
2.216AspSer: 2.216 ± 0.039
2.997AspThr: 2.997 ± 0.045
5.121AspVal: 5.121 ± 0.056
1.014AspTrp: 1.014 ± 0.025
1.128AspTyr: 1.128 ± 0.024
0.0AspXaa: 0.0 ± 0.0
Glu
6.417GluAla: 6.417 ± 0.086
0.334GluCys: 0.334 ± 0.014
1.994GluAsp: 1.994 ± 0.037
2.509GluGlu: 2.509 ± 0.051
1.408GluPhe: 1.408 ± 0.029
3.215GluGly: 3.215 ± 0.051
1.283GluHis: 1.283 ± 0.032
2.12GluIle: 2.12 ± 0.04
0.993GluLys: 0.993 ± 0.028
6.399GluLeu: 6.399 ± 0.079
0.786GluMet: 0.786 ± 0.026
0.826GluAsn: 0.826 ± 0.024
3.273GluPro: 3.273 ± 0.052
2.097GluGln: 2.097 ± 0.044
5.059GluArg: 5.059 ± 0.07
2.048GluSer: 2.048 ± 0.038
2.448GluThr: 2.448 ± 0.04
4.705GluVal: 4.705 ± 0.065
0.699GluTrp: 0.699 ± 0.023
1.067GluTyr: 1.067 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.747PheAla: 3.747 ± 0.049
0.247PheCys: 0.247 ± 0.011
1.937PheAsp: 1.937 ± 0.032
1.131PheGlu: 1.131 ± 0.025
0.83PhePhe: 0.83 ± 0.026
2.919PheGly: 2.919 ± 0.047
0.573PheHis: 0.573 ± 0.018
0.661PheIle: 0.661 ± 0.022
0.372PheLys: 0.372 ± 0.014
2.507PheLeu: 2.507 ± 0.046
0.309PheMet: 0.309 ± 0.014
0.569PheAsn: 0.569 ± 0.022
1.309PhePro: 1.309 ± 0.03
0.643PheGln: 0.643 ± 0.023
1.886PheArg: 1.886 ± 0.034
1.273PheSer: 1.273 ± 0.031
1.956PheThr: 1.956 ± 0.036
2.459PheVal: 2.459 ± 0.038
0.441PheTrp: 0.441 ± 0.017
0.6PheTyr: 0.6 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
11.067GlyAla: 11.067 ± 0.095
0.835GlyCys: 0.835 ± 0.022
5.27GlyAsp: 5.27 ± 0.061
4.722GlyGlu: 4.722 ± 0.064
2.796GlyPhe: 2.796 ± 0.044
8.958GlyGly: 8.958 ± 0.1
2.21GlyHis: 2.21 ± 0.04
3.045GlyIle: 3.045 ± 0.044
1.846GlyLys: 1.846 ± 0.038
9.414GlyLeu: 9.414 ± 0.08
1.915GlyMet: 1.915 ± 0.033
1.826GlyAsn: 1.826 ± 0.041
5.358GlyPro: 5.358 ± 0.056
2.819GlyGln: 2.819 ± 0.041
8.606GlyArg: 8.606 ± 0.083
4.731GlySer: 4.731 ± 0.061
5.936GlyThr: 5.936 ± 0.062
8.393GlyVal: 8.393 ± 0.07
1.992GlyTrp: 1.992 ± 0.036
2.46GlyTyr: 2.46 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.496HisAla: 2.496 ± 0.042
0.197HisCys: 0.197 ± 0.012
1.38HisAsp: 1.38 ± 0.03
1.025HisGlu: 1.025 ± 0.024
0.568HisPhe: 0.568 ± 0.02
2.211HisGly: 2.211 ± 0.036
0.669HisHis: 0.669 ± 0.02
0.514HisIle: 0.514 ± 0.019
0.246HisLys: 0.246 ± 0.013
2.475HisLeu: 2.475 ± 0.041
0.25HisMet: 0.25 ± 0.013
0.387HisAsn: 0.387 ± 0.015
1.775HisPro: 1.775 ± 0.035
0.61HisGln: 0.61 ± 0.021
2.245HisArg: 2.245 ± 0.036
0.862HisSer: 0.862 ± 0.025
1.118HisThr: 1.118 ± 0.027
1.725HisVal: 1.725 ± 0.037
0.363HisTrp: 0.363 ± 0.015
0.438HisTyr: 0.438 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
4.489IleAla: 4.489 ± 0.051
0.281IleCys: 0.281 ± 0.014
2.285IleAsp: 2.285 ± 0.039
1.739IleGlu: 1.739 ± 0.038
0.798IlePhe: 0.798 ± 0.024
3.294IleGly: 3.294 ± 0.05
0.539IleHis: 0.539 ± 0.016
0.907IleIle: 0.907 ± 0.028
0.58IleLys: 0.58 ± 0.023
2.32IleLeu: 2.32 ± 0.037
0.431IleMet: 0.431 ± 0.015
0.75IleAsn: 0.75 ± 0.025
1.6IlePro: 1.6 ± 0.032
0.691IleGln: 0.691 ± 0.02
2.42IleArg: 2.42 ± 0.036
1.628IleSer: 1.628 ± 0.031
2.102IleThr: 2.102 ± 0.036
2.751IleVal: 2.751 ± 0.044
0.4IleTrp: 0.4 ± 0.016
0.582IleTyr: 0.582 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
1.999LysAla: 1.999 ± 0.042
0.098LysCys: 0.098 ± 0.007
0.79LysAsp: 0.79 ± 0.023
0.766LysGlu: 0.766 ± 0.023
0.372LysPhe: 0.372 ± 0.016
1.23LysGly: 1.23 ± 0.03
0.318LysHis: 0.318 ± 0.013
0.762LysIle: 0.762 ± 0.026
0.46LysLys: 0.46 ± 0.024
1.688LysLeu: 1.688 ± 0.036
0.275LysMet: 0.275 ± 0.013
0.368LysAsn: 0.368 ± 0.017
1.078LysPro: 1.078 ± 0.03
0.562LysGln: 0.562 ± 0.019
1.224LysArg: 1.224 ± 0.028
0.835LysSer: 0.835 ± 0.023
1.009LysThr: 1.009 ± 0.029
1.528LysVal: 1.528 ± 0.038
0.21LysTrp: 0.21 ± 0.012
0.316LysTyr: 0.316 ± 0.013
0.0LysXaa: 0.0 ± 0.0
Leu
16.844LeuAla: 16.844 ± 0.138
0.737LeuCys: 0.737 ± 0.021
6.835LeuAsp: 6.835 ± 0.071
3.766LeuGlu: 3.766 ± 0.056
2.575LeuPhe: 2.575 ± 0.044
9.234LeuGly: 9.234 ± 0.085
2.212LeuHis: 2.212 ± 0.038
2.999LeuIle: 2.999 ± 0.049
1.335LeuLys: 1.335 ± 0.027
11.842LeuLeu: 11.842 ± 0.122
1.362LeuMet: 1.362 ± 0.028
1.664LeuAsn: 1.664 ± 0.031
6.767LeuPro: 6.767 ± 0.058
1.84LeuGln: 1.84 ± 0.035
9.593LeuArg: 9.593 ± 0.094
4.759LeuSer: 4.759 ± 0.051
6.95LeuThr: 6.95 ± 0.068
10.397LeuVal: 10.397 ± 0.088
1.259LeuTrp: 1.259 ± 0.027
1.686LeuTyr: 1.686 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.093MetAla: 2.093 ± 0.033
0.114MetCys: 0.114 ± 0.008
0.699MetAsp: 0.699 ± 0.02
0.577MetGlu: 0.577 ± 0.017
0.465MetPhe: 0.465 ± 0.016
1.093MetGly: 1.093 ± 0.029
0.287MetHis: 0.287 ± 0.012
0.641MetIle: 0.641 ± 0.02
0.322MetLys: 0.322 ± 0.012
1.646MetLeu: 1.646 ± 0.029
0.253MetMet: 0.253 ± 0.013
0.35MetAsn: 0.35 ± 0.016
1.043MetPro: 1.043 ± 0.023
0.402MetGln: 0.402 ± 0.018
1.461MetArg: 1.461 ± 0.03
1.149MetSer: 1.149 ± 0.025
1.507MetThr: 1.507 ± 0.03
1.303MetVal: 1.303 ± 0.029
0.204MetTrp: 0.204 ± 0.011
0.269MetTyr: 0.269 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.204AsnAla: 2.204 ± 0.038
0.152AsnCys: 0.152 ± 0.01
0.965AsnAsp: 0.965 ± 0.026
0.744AsnGlu: 0.744 ± 0.024
0.519AsnPhe: 0.519 ± 0.019
1.934AsnGly: 1.934 ± 0.049
0.398AsnHis: 0.398 ± 0.018
0.641AsnIle: 0.641 ± 0.022
0.332AsnLys: 0.332 ± 0.016
1.875AsnLeu: 1.875 ± 0.04
0.281AsnMet: 0.281 ± 0.015
0.502AsnAsn: 0.502 ± 0.023
1.508AsnPro: 1.508 ± 0.031
0.606AsnGln: 0.606 ± 0.022
1.42AsnArg: 1.42 ± 0.031
0.92AsnSer: 0.92 ± 0.028
1.129AsnThr: 1.129 ± 0.031
1.452AsnVal: 1.452 ± 0.029
0.337AsnTrp: 0.337 ± 0.016
0.462AsnTyr: 0.462 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
10.017ProAla: 10.017 ± 0.098
0.305ProCys: 0.305 ± 0.014
4.536ProAsp: 4.536 ± 0.054
3.765ProGlu: 3.765 ± 0.049
1.395ProPhe: 1.395 ± 0.026
6.687ProGly: 6.687 ± 0.075
1.248ProHis: 1.248 ± 0.028
1.535ProIle: 1.535 ± 0.031
0.981ProLys: 0.981 ± 0.027
5.231ProLeu: 5.231 ± 0.063
1.008ProMet: 1.008 ± 0.025
1.001ProAsn: 1.001 ± 0.027
4.306ProPro: 4.306 ± 0.066
1.628ProGln: 1.628 ± 0.035
4.219ProArg: 4.219 ± 0.058
2.958ProSer: 2.958 ± 0.048
4.058ProThr: 4.058 ± 0.048
6.174ProVal: 6.174 ± 0.068
0.985ProTrp: 0.985 ± 0.026
1.308ProTyr: 1.308 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.625GlnAla: 3.625 ± 0.05
0.157GlnCys: 0.157 ± 0.01
1.046GlnAsp: 1.046 ± 0.025
1.153GlnGlu: 1.153 ± 0.029
0.703GlnPhe: 0.703 ± 0.024
1.848GlnGly: 1.848 ± 0.033
0.591GlnHis: 0.591 ± 0.019
1.054GlnIle: 1.054 ± 0.024
0.431GlnLys: 0.431 ± 0.019
3.21GlnLeu: 3.21 ± 0.049
0.416GlnMet: 0.416 ± 0.015
0.482GlnAsn: 0.482 ± 0.018
1.847GlnPro: 1.847 ± 0.034
1.184GlnGln: 1.184 ± 0.04
2.802GlnArg: 2.802 ± 0.045
1.11GlnSer: 1.11 ± 0.029
1.324GlnThr: 1.324 ± 0.029
2.841GlnVal: 2.841 ± 0.043
0.547GlnTrp: 0.547 ± 0.019
0.55GlnTyr: 0.55 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
10.759ArgAla: 10.759 ± 0.096
0.667ArgCys: 0.667 ± 0.022
4.743ArgAsp: 4.743 ± 0.057
4.372ArgGlu: 4.372 ± 0.062
2.497ArgPhe: 2.497 ± 0.041
6.125ArgGly: 6.125 ± 0.066
2.378ArgHis: 2.378 ± 0.042
3.205ArgIle: 3.205 ± 0.043
1.257ArgLys: 1.257 ± 0.03
9.785ArgLeu: 9.785 ± 0.094
1.896ArgMet: 1.896 ± 0.031
1.525ArgAsn: 1.525 ± 0.036
5.786ArgPro: 5.786 ± 0.076
2.797ArgGln: 2.797 ± 0.041
9.411ArgArg: 9.411 ± 0.095
4.135ArgSer: 4.135 ± 0.059
5.048ArgThr: 5.048 ± 0.065
6.951ArgVal: 6.951 ± 0.07
1.793ArgTrp: 1.793 ± 0.034
2.154ArgTyr: 2.154 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.124SerAla: 6.124 ± 0.065
0.393SerCys: 0.393 ± 0.015
2.241SerAsp: 2.241 ± 0.034
1.844SerGlu: 1.844 ± 0.038
1.407SerPhe: 1.407 ± 0.031
5.395SerGly: 5.395 ± 0.066
0.865SerHis: 0.865 ± 0.023
1.448SerIle: 1.448 ± 0.031
0.721SerLys: 0.721 ± 0.024
4.19SerLeu: 4.19 ± 0.054
0.924SerMet: 0.924 ± 0.021
0.887SerAsn: 0.887 ± 0.027
3.146SerPro: 3.146 ± 0.049
1.117SerGln: 1.117 ± 0.028
3.777SerArg: 3.777 ± 0.052
2.322SerSer: 2.322 ± 0.045
3.143SerThr: 3.143 ± 0.054
4.022SerVal: 4.022 ± 0.055
0.917SerTrp: 0.917 ± 0.026
1.212SerTyr: 1.212 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
8.915ThrAla: 8.915 ± 0.079
0.441ThrCys: 0.441 ± 0.017
3.592ThrAsp: 3.592 ± 0.048
2.934ThrGlu: 2.934 ± 0.047
1.611ThrPhe: 1.611 ± 0.03
6.905ThrGly: 6.905 ± 0.08
1.086ThrHis: 1.086 ± 0.025
1.942ThrIle: 1.942 ± 0.039
0.937ThrLys: 0.937 ± 0.028
5.977ThrLeu: 5.977 ± 0.063
0.985ThrMet: 0.985 ± 0.024
1.171ThrAsn: 1.171 ± 0.035
4.291ThrPro: 4.291 ± 0.054
1.266ThrGln: 1.266 ± 0.028
4.338ThrArg: 4.338 ± 0.048
2.953ThrSer: 2.953 ± 0.052
3.854ThrThr: 3.854 ± 0.064
6.6ThrVal: 6.6 ± 0.076
0.979ThrTrp: 0.979 ± 0.025
1.306ThrTyr: 1.306 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
13.22ValAla: 13.22 ± 0.12
0.745ValCys: 0.745 ± 0.021
6.118ValAsp: 6.118 ± 0.064
4.822ValGlu: 4.822 ± 0.066
2.389ValPhe: 2.389 ± 0.043
7.95ValGly: 7.95 ± 0.073
1.851ValHis: 1.851 ± 0.036
2.747ValIle: 2.747 ± 0.039
1.381ValLys: 1.381 ± 0.033
10.064ValLeu: 10.064 ± 0.088
1.175ValMet: 1.175 ± 0.03
1.826ValAsn: 1.826 ± 0.037
5.684ValPro: 5.684 ± 0.069
1.958ValGln: 1.958 ± 0.032
7.718ValArg: 7.718 ± 0.076
4.343ValSer: 4.343 ± 0.054
6.461ValThr: 6.461 ± 0.071
9.481ValVal: 9.481 ± 0.099
1.176ValTrp: 1.176 ± 0.031
1.566ValTyr: 1.566 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.746TrpAla: 1.746 ± 0.036
0.166TrpCys: 0.166 ± 0.009
0.772TrpAsp: 0.772 ± 0.022
0.604TrpGlu: 0.604 ± 0.021
0.518TrpPhe: 0.518 ± 0.017
1.014TrpGly: 1.014 ± 0.027
0.419TrpHis: 0.419 ± 0.015
0.525TrpIle: 0.525 ± 0.02
0.277TrpLys: 0.277 ± 0.014
1.907TrpLeu: 1.907 ± 0.03
0.284TrpMet: 0.284 ± 0.013
0.438TrpAsn: 0.438 ± 0.018
0.959TrpPro: 0.959 ± 0.026
0.668TrpGln: 0.668 ± 0.02
1.765TrpArg: 1.765 ± 0.037
1.082TrpSer: 1.082 ± 0.027
1.1TrpThr: 1.1 ± 0.03
1.149TrpVal: 1.149 ± 0.026
0.408TrpTrp: 0.408 ± 0.016
0.414TrpTyr: 0.414 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.933TyrAla: 2.933 ± 0.044
0.174TyrCys: 0.174 ± 0.011
1.449TyrAsp: 1.449 ± 0.032
1.045TyrGlu: 1.045 ± 0.027
0.626TyrPhe: 0.626 ± 0.019
2.13TyrGly: 2.13 ± 0.039
0.487TyrHis: 0.487 ± 0.017
0.411TyrIle: 0.411 ± 0.02
0.3TyrLys: 0.3 ± 0.015
2.337TyrLeu: 2.337 ± 0.043
0.189TyrMet: 0.189 ± 0.01
0.425TyrAsn: 0.425 ± 0.019
1.233TyrPro: 1.233 ± 0.028
0.698TyrGln: 0.698 ± 0.02
2.036TyrArg: 2.036 ± 0.034
0.864TyrSer: 0.864 ± 0.023
1.129TyrThr: 1.129 ± 0.03
1.745TyrVal: 1.745 ± 0.036
0.356TyrTrp: 0.356 ± 0.014
0.479TyrTyr: 0.479 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5344 proteins (1713476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski