Capybara microvirus Cap1_SP_76
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7K6|A0A4P8W7K6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_76 OX=2584790 PE=4 SV=1
MM1 pKa = 7.01 EE2 pKa = 4.34 TVFDD6 pKa = 3.96 KK7 pKa = 11.35 LKK9 pKa = 10.56 DD10 pKa = 3.19 IRR12 pKa = 11.84 LPEE15 pKa = 3.86 EE16 pKa = 4.42 RR17 pKa = 11.84 EE18 pKa = 3.73 ASEE21 pKa = 3.74 YY22 pKa = 10.89 DD23 pKa = 3.37 INIMVAKK30 pKa = 10.1 GVRR33 pKa = 11.84 GDD35 pKa = 3.43 NGIAEE40 pKa = 4.19 DD41 pKa = 4.17 QYY43 pKa = 12.18 NGITDD48 pKa = 3.29 VSRR51 pKa = 11.84 ILGLVTDD58 pKa = 5.08 NFQAMDD64 pKa = 3.48 SLAYY68 pKa = 9.4 INRR71 pKa = 11.84 YY72 pKa = 8.49 GKK74 pKa = 10.16 AAEE77 pKa = 4.28 AQQQQSS83 pKa = 3.13
Molecular weight: 9.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.524
IPC2_protein 4.444
IPC_protein 4.317
Toseland 4.139
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.19
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.228
Nozaki 4.393
DTASelect 4.584
Thurlkill 4.164
EMBOSS 4.202
Sillero 4.431
Patrickios 3.973
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.365
Protein with the highest isoelectric point:
>tr|A0A4P8W7K6|A0A4P8W7K6_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_76 OX=2584790 PE=4 SV=1
MM1 pKa = 8.09 IEE3 pKa = 4.94 DD4 pKa = 4.05 IEE6 pKa = 4.6 SLGDD10 pKa = 3.18 QSMQYY15 pKa = 9.59 FQKK18 pKa = 10.24 KK19 pKa = 8.92 ANYY22 pKa = 8.29 RR23 pKa = 11.84 ASKK26 pKa = 8.29 TKK28 pKa = 10.42 SVVGGVLSAVASAIASIFATPAAGAAVGAGASGAIAASLASVAGASGVVTAAAEE82 pKa = 4.12 KK83 pKa = 10.25 GASISANAVKK93 pKa = 10.29 AATAFDD99 pKa = 3.98 KK100 pKa = 10.99 VDD102 pKa = 4.11 KK103 pKa = 10.87 IRR105 pKa = 11.84 TAVGNPRR112 pKa = 11.84 QWISNGIARR121 pKa = 11.84 GILNSNRR128 pKa = 11.84 GLLTAAGNYY137 pKa = 6.7 TMRR140 pKa = 11.84 SATQASMDD148 pKa = 3.79 AVGKK152 pKa = 10.57 AILNIPKK159 pKa = 10.43 LGGLL163 pKa = 3.79
Molecular weight: 16.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.111
IPC2_protein 9.443
IPC_protein 9.458
Toseland 10.335
ProMoST 9.882
Dawson 10.452
Bjellqvist 10.058
Wikipedia 10.584
Rodwell 11.067
Grimsley 10.496
Solomon 10.482
Lehninger 10.467
Nozaki 10.292
DTASelect 10.058
Thurlkill 10.335
EMBOSS 10.716
Sillero 10.365
Patrickios 10.818
IPC_peptide 10.496
IPC2_peptide 8.478
IPC2.peptide.svr19 8.548
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1638
83
514
273.0
30.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.341 ± 2.3
1.038 ± 0.488
6.227 ± 0.638
4.518 ± 0.727
5.311 ± 0.905
5.983 ± 0.937
2.015 ± 0.513
5.433 ± 0.84
5.067 ± 0.736
9.341 ± 0.951
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.503 ± 0.555
4.701 ± 0.588
3.968 ± 0.954
5.556 ± 0.503
5.433 ± 0.911
8.547 ± 0.955
4.884 ± 0.427
5.189 ± 0.601
0.916 ± 0.154
4.029 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here