Amino acid dipepetide frequency for Desulfotomaculum reducens (strain MI-1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.139AlaAla: 7.139 ± 0.115	0.905AlaCys: 0.905 ± 0.03	3.488AlaAsp: 3.488 ± 0.06	5.281AlaGlu: 5.281 ± 0.073	2.695AlaPhe: 2.695 ± 0.054	6.333AlaGly: 6.333 ± 0.099	1.249AlaHis: 1.249 ± 0.043	5.724AlaIle: 5.724 ± 0.079	5.194AlaLys: 5.194 ± 0.083	8.148AlaLeu: 8.148 ± 0.098	2.299AlaMet: 2.299 ± 0.05	2.959AlaAsn: 2.959 ± 0.061	2.336AlaPro: 2.336 ± 0.05	2.863AlaGln: 2.863 ± 0.06	3.905AlaArg: 3.905 ± 0.073	3.93AlaSer: 3.93 ± 0.068	4.019AlaThr: 4.019 ± 0.077	6.27AlaVal: 6.27 ± 0.103	0.635AlaTrp: 0.635 ± 0.027	2.163AlaTyr: 2.163 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.719CysAla: 0.719 ± 0.029	0.227CysCys: 0.227 ± 0.017	0.493CysAsp: 0.493 ± 0.02	0.561CysGlu: 0.561 ± 0.025	0.438CysPhe: 0.438 ± 0.022	1.216CysGly: 1.216 ± 0.041	0.286CysHis: 0.286 ± 0.018	0.714CysIle: 0.714 ± 0.027	0.632CysLys: 0.632 ± 0.024	1.1CysLeu: 1.1 ± 0.031	0.293CysMet: 0.293 ± 0.015	0.506CysAsn: 0.506 ± 0.022	0.719CysPro: 0.719 ± 0.031	0.554CysGln: 0.554 ± 0.022	0.614CysArg: 0.614 ± 0.024	0.749CysSer: 0.749 ± 0.028	0.579CysThr: 0.579 ± 0.029	0.668CysVal: 0.668 ± 0.03	0.111CysTrp: 0.111 ± 0.01	0.372CysTyr: 0.372 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.227AspAla: 3.227 ± 0.06	0.565AspCys: 0.565 ± 0.027	1.996AspAsp: 1.996 ± 0.056	3.187AspGlu: 3.187 ± 0.066	2.179AspPhe: 2.179 ± 0.052	3.121AspGly: 3.121 ± 0.07	0.925AspHis: 0.925 ± 0.032	4.342AspIle: 4.342 ± 0.063	3.2AspLys: 3.2 ± 0.062	5.13AspLeu: 5.13 ± 0.079	1.359AspMet: 1.359 ± 0.038	2.017AspAsn: 2.017 ± 0.056	2.243AspPro: 2.243 ± 0.052	1.862AspGln: 1.862 ± 0.043	2.346AspArg: 2.346 ± 0.049	2.27AspSer: 2.27 ± 0.049	2.418AspThr: 2.418 ± 0.053	3.348AspVal: 3.348 ± 0.069	0.547AspTrp: 0.547 ± 0.024	1.905AspTyr: 1.905 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	5.363GluAla: 5.363 ± 0.092	0.571GluCys: 0.571 ± 0.02	3.205GluAsp: 3.205 ± 0.065	5.691GluGlu: 5.691 ± 0.104	2.324GluPhe: 2.324 ± 0.047	4.377GluGly: 4.377 ± 0.076	1.127GluHis: 1.127 ± 0.036	5.618GluIle: 5.618 ± 0.095	5.567GluLys: 5.567 ± 0.085	6.99GluLeu: 6.99 ± 0.092	2.099GluMet: 2.099 ± 0.046	2.996GluAsn: 2.996 ± 0.056	2.052GluPro: 2.052 ± 0.046	3.175GluGln: 3.175 ± 0.067	3.506GluArg: 3.506 ± 0.071	2.822GluSer: 2.822 ± 0.057	3.252GluThr: 3.252 ± 0.061	5.422GluVal: 5.422 ± 0.094	0.519GluTrp: 0.519 ± 0.022	2.102GluTyr: 2.102 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.774PheAla: 2.774 ± 0.066	0.517PheCys: 0.517 ± 0.022	1.962PheAsp: 1.962 ± 0.046	2.159PheGlu: 2.159 ± 0.049	1.838PhePhe: 1.838 ± 0.059	3.009PheGly: 3.009 ± 0.07	0.724PheHis: 0.724 ± 0.027	3.045PheIle: 3.045 ± 0.066	2.3PheLys: 2.3 ± 0.048	3.929PheLeu: 3.929 ± 0.076	1.052PheMet: 1.052 ± 0.037	1.888PheAsn: 1.888 ± 0.04	1.484PhePro: 1.484 ± 0.038	1.217PheGln: 1.217 ± 0.037	1.604PheArg: 1.604 ± 0.042	2.656PheSer: 2.656 ± 0.053	2.224PheThr: 2.224 ± 0.051	2.517PheVal: 2.517 ± 0.058	0.411PheTrp: 0.411 ± 0.021	1.42PheTyr: 1.42 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	5.454GlyAla: 5.454 ± 0.093	1.067GlyCys: 1.067 ± 0.033	3.383GlyAsp: 3.383 ± 0.072	4.545GlyGlu: 4.545 ± 0.08	3.181GlyPhe: 3.181 ± 0.071	5.396GlyGly: 5.396 ± 0.099	1.401GlyHis: 1.401 ± 0.038	6.063GlyIle: 6.063 ± 0.096	5.295GlyLys: 5.295 ± 0.078	7.746GlyLeu: 7.746 ± 0.1	2.195GlyMet: 2.195 ± 0.052	2.892GlyAsn: 2.892 ± 0.059	2.15GlyPro: 2.15 ± 0.045	2.972GlyGln: 2.972 ± 0.057	3.345GlyArg: 3.345 ± 0.061	4.027GlySer: 4.027 ± 0.074	4.197GlyThr: 4.197 ± 0.07	5.795GlyVal: 5.795 ± 0.09	0.816GlyTrp: 0.816 ± 0.033	2.851GlyTyr: 2.851 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.155HisAla: 1.155 ± 0.032	0.334HisCys: 0.334 ± 0.019	0.813HisAsp: 0.813 ± 0.028	0.997HisGlu: 0.997 ± 0.034	0.844HisPhe: 0.844 ± 0.031	1.341HisGly: 1.341 ± 0.04	0.503HisHis: 0.503 ± 0.026	1.381HisIle: 1.381 ± 0.04	0.992HisLys: 0.992 ± 0.028	1.881HisLeu: 1.881 ± 0.051	0.501HisMet: 0.501 ± 0.025	0.787HisAsn: 0.787 ± 0.027	1.022HisPro: 1.022 ± 0.035	0.796HisGln: 0.796 ± 0.026	0.959HisArg: 0.959 ± 0.035	1.028HisSer: 1.028 ± 0.035	0.877HisThr: 0.877 ± 0.029	1.073HisVal: 1.073 ± 0.032	0.254HisTrp: 0.254 ± 0.016	0.76HisTyr: 0.76 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.696IleAla: 5.696 ± 0.085	0.881IleCys: 0.881 ± 0.026	3.748IleAsp: 3.748 ± 0.062	4.703IleGlu: 4.703 ± 0.078	2.897IlePhe: 2.897 ± 0.06	5.215IleGly: 5.215 ± 0.088	1.394IleHis: 1.394 ± 0.039	5.626IleIle: 5.626 ± 0.083	5.236IleLys: 5.236 ± 0.095	7.189IleLeu: 7.189 ± 0.086	1.88IleMet: 1.88 ± 0.043	3.599IleAsn: 3.599 ± 0.064	3.443IlePro: 3.443 ± 0.061	2.563IleGln: 2.563 ± 0.056	3.359IleArg: 3.359 ± 0.055	4.757IleSer: 4.757 ± 0.068	4.4IleThr: 4.4 ± 0.076	4.802IleVal: 4.802 ± 0.071	0.555IleTrp: 0.555 ± 0.028	2.229IleTyr: 2.229 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	4.813LysAla: 4.813 ± 0.075	0.579LysCys: 0.579 ± 0.023	3.651LysAsp: 3.651 ± 0.076	5.877LysGlu: 5.877 ± 0.094	1.999LysPhe: 1.999 ± 0.042	4.582LysGly: 4.582 ± 0.074	1.089LysHis: 1.089 ± 0.035	4.899LysIle: 4.899 ± 0.081	4.933LysLys: 4.933 ± 0.083	6.116LysLeu: 6.116 ± 0.083	1.864LysMet: 1.864 ± 0.04	3.117LysAsn: 3.117 ± 0.066	2.489LysPro: 2.489 ± 0.051	2.68LysGln: 2.68 ± 0.057	2.958LysArg: 2.958 ± 0.056	3.161LysSer: 3.161 ± 0.063	3.487LysThr: 3.487 ± 0.059	5.078LysVal: 5.078 ± 0.084	0.519LysTrp: 0.519 ± 0.024	2.209LysTyr: 2.209 ± 0.054	0.0LysXaa: 0.0 ± 0.0
Leu	9.135LeuAla: 9.135 ± 0.101	1.105LeuCys: 1.105 ± 0.033	4.947LeuAsp: 4.947 ± 0.068	7.01LeuGlu: 7.01 ± 0.103	3.687LeuPhe: 3.687 ± 0.071	7.791LeuGly: 7.791 ± 0.102	1.636LeuHis: 1.636 ± 0.042	6.499LeuIle: 6.499 ± 0.095	6.687LeuLys: 6.687 ± 0.095	10.183LeuLeu: 10.183 ± 0.143	2.579LeuMet: 2.579 ± 0.054	4.318LeuAsn: 4.318 ± 0.078	4.502LeuPro: 4.502 ± 0.065	3.728LeuGln: 3.728 ± 0.067	4.554LeuArg: 4.554 ± 0.079	6.244LeuSer: 6.244 ± 0.101	5.496LeuThr: 5.496 ± 0.077	7.402LeuVal: 7.402 ± 0.102	0.884LeuTrp: 0.884 ± 0.03	2.912LeuTyr: 2.912 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.799MetAla: 2.799 ± 0.055	0.212MetCys: 0.212 ± 0.014	1.491MetAsp: 1.491 ± 0.039	1.867MetGlu: 1.867 ± 0.046	0.92MetPhe: 0.92 ± 0.029	2.25MetGly: 2.25 ± 0.051	0.406MetHis: 0.406 ± 0.021	1.751MetIle: 1.751 ± 0.042	1.767MetLys: 1.767 ± 0.048	2.606MetLeu: 2.606 ± 0.055	0.717MetMet: 0.717 ± 0.027	1.173MetAsn: 1.173 ± 0.035	1.073MetPro: 1.073 ± 0.031	0.977MetGln: 0.977 ± 0.033	1.1MetArg: 1.1 ± 0.026	1.511MetSer: 1.511 ± 0.041	1.468MetThr: 1.468 ± 0.04	2.321MetVal: 2.321 ± 0.047	0.192MetTrp: 0.192 ± 0.015	0.622MetTyr: 0.622 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.657AsnAla: 2.657 ± 0.054	0.596AsnCys: 0.596 ± 0.025	1.827AsnAsp: 1.827 ± 0.047	2.4AsnGlu: 2.4 ± 0.055	1.683AsnPhe: 1.683 ± 0.052	2.846AsnGly: 2.846 ± 0.063	0.894AsnHis: 0.894 ± 0.029	3.677AsnIle: 3.677 ± 0.068	3.002AsnLys: 3.002 ± 0.054	4.506AsnLeu: 4.506 ± 0.071	1.166AsnMet: 1.166 ± 0.036	2.13AsnAsn: 2.13 ± 0.054	2.391AsnPro: 2.391 ± 0.056	1.933AsnGln: 1.933 ± 0.044	2.07AsnArg: 2.07 ± 0.051	2.3AsnSer: 2.3 ± 0.057	2.312AsnThr: 2.312 ± 0.046	2.71AsnVal: 2.71 ± 0.059	0.476AsnTrp: 0.476 ± 0.022	1.453AsnTyr: 1.453 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.91ProAla: 2.91 ± 0.066	0.403ProCys: 0.403 ± 0.019	2.124ProAsp: 2.124 ± 0.044	3.54ProGlu: 3.54 ± 0.064	1.691ProPhe: 1.691 ± 0.035	3.383ProGly: 3.383 ± 0.064	0.803ProHis: 0.803 ± 0.03	2.458ProIle: 2.458 ± 0.045	2.266ProLys: 2.266 ± 0.05	3.802ProLeu: 3.802 ± 0.067	1.057ProMet: 1.057 ± 0.038	1.632ProAsn: 1.632 ± 0.042	1.411ProPro: 1.411 ± 0.048	1.575ProGln: 1.575 ± 0.045	1.617ProArg: 1.617 ± 0.041	2.175ProSer: 2.175 ± 0.045	2.117ProThr: 2.117 ± 0.048	3.625ProVal: 3.625 ± 0.069	0.447ProTrp: 0.447 ± 0.024	1.314ProTyr: 1.314 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	3.295GlnAla: 3.295 ± 0.063	0.403GlnCys: 0.403 ± 0.022	1.84GlnAsp: 1.84 ± 0.044	3.284GlnGlu: 3.284 ± 0.069	1.246GlnPhe: 1.246 ± 0.035	2.921GlnGly: 2.921 ± 0.059	0.702GlnHis: 0.702 ± 0.027	2.741GlnIle: 2.741 ± 0.051	2.533GlnLys: 2.533 ± 0.048	3.821GlnLeu: 3.821 ± 0.072	1.083GlnMet: 1.083 ± 0.033	1.622GlnAsn: 1.622 ± 0.039	1.515GlnPro: 1.515 ± 0.04	1.975GlnGln: 1.975 ± 0.063	2.17GlnArg: 2.17 ± 0.05	1.906GlnSer: 1.906 ± 0.051	1.794GlnThr: 1.794 ± 0.045	3.084GlnVal: 3.084 ± 0.052	0.44GlnTrp: 0.44 ± 0.022	1.304GlnTyr: 1.304 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	3.262ArgAla: 3.262 ± 0.057	0.518ArgCys: 0.518 ± 0.021	2.32ArgAsp: 2.32 ± 0.051	3.692ArgGlu: 3.692 ± 0.066	1.802ArgPhe: 1.802 ± 0.046	3.043ArgGly: 3.043 ± 0.056	0.877ArgHis: 0.877 ± 0.032	3.414ArgIle: 3.414 ± 0.058	3.062ArgLys: 3.062 ± 0.068	4.718ArgLeu: 4.718 ± 0.08	1.419ArgMet: 1.419 ± 0.038	1.903ArgAsn: 1.903 ± 0.039	1.823ArgPro: 1.823 ± 0.047	2.188ArgGln: 2.188 ± 0.054	2.426ArgArg: 2.426 ± 0.067	2.326ArgSer: 2.326 ± 0.05	2.181ArgThr: 2.181 ± 0.051	3.58ArgVal: 3.58 ± 0.069	0.452ArgTrp: 0.452 ± 0.022	1.664ArgTyr: 1.664 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.81SerAla: 3.81 ± 0.072	0.645SerCys: 0.645 ± 0.027	2.48SerAsp: 2.48 ± 0.057	3.326SerGlu: 3.326 ± 0.082	2.445SerPhe: 2.445 ± 0.047	4.57SerGly: 4.57 ± 0.063	1.092SerHis: 1.092 ± 0.034	4.013SerIle: 4.013 ± 0.072	3.29SerLys: 3.29 ± 0.057	5.997SerLeu: 5.997 ± 0.084	1.555SerMet: 1.555 ± 0.04	2.173SerAsn: 2.173 ± 0.054	2.298SerPro: 2.298 ± 0.05	2.261SerGln: 2.261 ± 0.051	2.633SerArg: 2.633 ± 0.053	3.199SerSer: 3.199 ± 0.066	2.841SerThr: 2.841 ± 0.056	3.746SerVal: 3.746 ± 0.07	0.58SerTrp: 0.58 ± 0.025	1.777SerTyr: 1.777 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.178ThrAla: 4.178 ± 0.072	0.576ThrCys: 0.576 ± 0.027	2.42ThrAsp: 2.42 ± 0.051	3.14ThrGlu: 3.14 ± 0.063	2.162ThrPhe: 2.162 ± 0.051	4.879ThrGly: 4.879 ± 0.082	0.97ThrHis: 0.97 ± 0.03	3.93ThrIle: 3.93 ± 0.069	2.985ThrLys: 2.985 ± 0.059	5.291ThrLeu: 5.291 ± 0.076	1.292ThrMet: 1.292 ± 0.039	2.311ThrAsn: 2.311 ± 0.057	2.571ThrPro: 2.571 ± 0.051	1.689ThrGln: 1.689 ± 0.04	2.187ThrArg: 2.187 ± 0.057	2.929ThrSer: 2.929 ± 0.059	2.984ThrThr: 2.984 ± 0.054	4.244ThrVal: 4.244 ± 0.069	0.493ThrTrp: 0.493 ± 0.022	1.595ThrTyr: 1.595 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	6.319ValAla: 6.319 ± 0.113	0.856ValCys: 0.856 ± 0.029	3.914ValAsp: 3.914 ± 0.072	5.084ValGlu: 5.084 ± 0.073	2.836ValPhe: 2.836 ± 0.058	5.345ValGly: 5.345 ± 0.091	1.189ValHis: 1.189 ± 0.032	5.516ValIle: 5.516 ± 0.079	4.749ValLys: 4.749 ± 0.074	7.391ValLeu: 7.391 ± 0.095	1.886ValMet: 1.886 ± 0.045	3.238ValAsn: 3.238 ± 0.054	3.096ValPro: 3.096 ± 0.061	2.553ValGln: 2.553 ± 0.048	3.082ValArg: 3.082 ± 0.057	4.401ValSer: 4.401 ± 0.068	4.128ValThr: 4.128 ± 0.064	6.025ValVal: 6.025 ± 0.08	0.611ValTrp: 0.611 ± 0.029	2.09ValTyr: 2.09 ± 0.05	0.0ValXaa: 0.0 ± 0.0
Trp	0.653TrpAla: 0.653 ± 0.028	0.125TrpCys: 0.125 ± 0.011	0.521TrpAsp: 0.521 ± 0.022	0.654TrpGlu: 0.654 ± 0.028	0.426TrpPhe: 0.426 ± 0.02	0.65TrpGly: 0.65 ± 0.025	0.229TrpHis: 0.229 ± 0.016	0.544TrpIle: 0.544 ± 0.021	0.481TrpLys: 0.481 ± 0.024	1.144TrpLeu: 1.144 ± 0.034	0.253TrpMet: 0.253 ± 0.016	0.366TrpAsn: 0.366 ± 0.02	0.317TrpPro: 0.317 ± 0.019	0.593TrpGln: 0.593 ± 0.025	0.455TrpArg: 0.455 ± 0.021	0.515TrpSer: 0.515 ± 0.023	0.4TrpThr: 0.4 ± 0.022	0.653TrpVal: 0.653 ± 0.027	0.13TrpTrp: 0.13 ± 0.012	0.308TrpTyr: 0.308 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.177TyrAla: 2.177 ± 0.053	0.466TyrCys: 0.466 ± 0.024	1.564TyrAsp: 1.564 ± 0.047	1.781TyrGlu: 1.781 ± 0.043	1.437TyrPhe: 1.437 ± 0.043	2.504TyrGly: 2.504 ± 0.046	0.8TyrHis: 0.8 ± 0.029	2.218TyrIle: 2.218 ± 0.045	1.927TyrLys: 1.927 ± 0.051	3.609TyrLeu: 3.609 ± 0.065	0.676TyrMet: 0.676 ± 0.029	1.398TyrAsn: 1.398 ± 0.043	1.416TyrPro: 1.416 ± 0.043	1.528TyrGln: 1.528 ± 0.043	1.779TyrArg: 1.779 ± 0.044	1.828TyrSer: 1.828 ± 0.053	1.651TyrThr: 1.651 ± 0.041	1.946TyrVal: 1.946 ± 0.043	0.346TyrTrp: 0.346 ± 0.021	1.297TyrTyr: 1.297 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3220 proteins (980491 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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