Amino acid dipepetide frequency for Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.884AlaAla: 11.884 ± 0.173	1.38AlaCys: 1.38 ± 0.043	4.114AlaAsp: 4.114 ± 0.079	6.551AlaGlu: 6.551 ± 0.088	3.292AlaPhe: 3.292 ± 0.066	11.619AlaGly: 11.619 ± 0.159	1.313AlaHis: 1.313 ± 0.044	4.656AlaIle: 4.656 ± 0.076	3.724AlaLys: 3.724 ± 0.068	9.849AlaLeu: 9.849 ± 0.109	2.228AlaMet: 2.228 ± 0.055	2.204AlaAsn: 2.204 ± 0.054	3.104AlaPro: 3.104 ± 0.069	2.447AlaGln: 2.447 ± 0.056	7.051AlaArg: 7.051 ± 0.107	4.106AlaSer: 4.106 ± 0.07	3.269AlaThr: 3.269 ± 0.065	9.266AlaVal: 9.266 ± 0.147	0.905AlaTrp: 0.905 ± 0.037	2.36AlaTyr: 2.36 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.944CysAla: 0.944 ± 0.036	0.235CysCys: 0.235 ± 0.019	0.513CysAsp: 0.513 ± 0.027	0.606CysGlu: 0.606 ± 0.025	0.519CysPhe: 0.519 ± 0.025	1.506CysGly: 1.506 ± 0.043	0.27CysHis: 0.27 ± 0.021	0.609CysIle: 0.609 ± 0.029	0.493CysLys: 0.493 ± 0.023	1.401CysLeu: 1.401 ± 0.043	0.258CysMet: 0.258 ± 0.018	0.401CysAsn: 0.401 ± 0.022	0.842CysPro: 0.842 ± 0.036	0.345CysGln: 0.345 ± 0.021	1.172CysArg: 1.172 ± 0.038	0.769CysSer: 0.769 ± 0.033	0.572CysThr: 0.572 ± 0.027	0.696CysVal: 0.696 ± 0.032	0.139CysTrp: 0.139 ± 0.014	0.398CysTyr: 0.398 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.502AspAla: 3.502 ± 0.075	0.588AspCys: 0.588 ± 0.026	1.839AspAsp: 1.839 ± 0.051	3.26AspGlu: 3.26 ± 0.068	2.129AspPhe: 2.129 ± 0.051	3.988AspGly: 3.988 ± 0.074	0.724AspHis: 0.724 ± 0.03	3.397AspIle: 3.397 ± 0.063	2.197AspLys: 2.197 ± 0.053	5.091AspLeu: 5.091 ± 0.078	1.119AspMet: 1.119 ± 0.037	1.313AspAsn: 1.313 ± 0.039	2.603AspPro: 2.603 ± 0.055	1.251AspGln: 1.251 ± 0.042	3.287AspArg: 3.287 ± 0.074	2.104AspSer: 2.104 ± 0.051	1.976AspThr: 1.976 ± 0.07	3.384AspVal: 3.384 ± 0.063	0.54AspTrp: 0.54 ± 0.024	1.74AspTyr: 1.74 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.908GluAla: 6.908 ± 0.109	0.619GluCys: 0.619 ± 0.03	3.118GluAsp: 3.118 ± 0.057	6.37GluGlu: 6.37 ± 0.103	2.325GluPhe: 2.325 ± 0.057	4.975GluGly: 4.975 ± 0.075	1.155GluHis: 1.155 ± 0.036	5.369GluIle: 5.369 ± 0.094	5.844GluLys: 5.844 ± 0.104	7.322GluLeu: 7.322 ± 0.118	2.12GluMet: 2.12 ± 0.051	2.807GluAsn: 2.807 ± 0.056	2.33GluPro: 2.33 ± 0.055	2.487GluGln: 2.487 ± 0.074	4.438GluArg: 4.438 ± 0.078	2.82GluSer: 2.82 ± 0.058	2.99GluThr: 2.99 ± 0.056	5.626GluVal: 5.626 ± 0.09	0.611GluTrp: 0.611 ± 0.031	2.07GluTyr: 2.07 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	3.227PheAla: 3.227 ± 0.068	0.637PheCys: 0.637 ± 0.024	1.971PheAsp: 1.971 ± 0.053	2.254PheGlu: 2.254 ± 0.047	1.893PhePhe: 1.893 ± 0.054	2.996PheGly: 2.996 ± 0.07	0.639PheHis: 0.639 ± 0.027	2.534PheIle: 2.534 ± 0.066	2.168PheLys: 2.168 ± 0.052	4.198PheLeu: 4.198 ± 0.073	0.938PheMet: 0.938 ± 0.033	1.563PheAsn: 1.563 ± 0.04	1.806PhePro: 1.806 ± 0.047	1.166PheGln: 1.166 ± 0.041	2.082PheArg: 2.082 ± 0.055	2.494PheSer: 2.494 ± 0.053	1.999PheThr: 1.999 ± 0.042	2.412PheVal: 2.412 ± 0.056	0.463PheTrp: 0.463 ± 0.024	1.443PheTyr: 1.443 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	7.236GlyAla: 7.236 ± 0.114	1.259GlyCys: 1.259 ± 0.047	3.653GlyAsp: 3.653 ± 0.063	5.856GlyGlu: 5.856 ± 0.086	3.337GlyPhe: 3.337 ± 0.063	7.07GlyGly: 7.07 ± 0.126	1.486GlyHis: 1.486 ± 0.046	5.617GlyIle: 5.617 ± 0.082	5.171GlyLys: 5.171 ± 0.083	9.015GlyLeu: 9.015 ± 0.121	2.204GlyMet: 2.204 ± 0.052	2.58GlyAsn: 2.58 ± 0.062	2.934GlyPro: 2.934 ± 0.065	2.749GlyGln: 2.749 ± 0.059	6.403GlyArg: 6.403 ± 0.094	4.314GlySer: 4.314 ± 0.072	4.167GlyThr: 4.167 ± 0.071	6.242GlyVal: 6.242 ± 0.106	0.915GlyTrp: 0.915 ± 0.032	2.893GlyTyr: 2.893 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.238HisAla: 1.238 ± 0.044	0.296HisCys: 0.296 ± 0.019	0.729HisAsp: 0.729 ± 0.03	0.903HisGlu: 0.903 ± 0.032	0.825HisPhe: 0.825 ± 0.028	1.471HisGly: 1.471 ± 0.052	0.425HisHis: 0.425 ± 0.026	0.987HisIle: 0.987 ± 0.035	0.695HisLys: 0.695 ± 0.028	1.923HisLeu: 1.923 ± 0.053	0.303HisMet: 0.303 ± 0.018	0.549HisAsn: 0.549 ± 0.028	1.132HisPro: 1.132 ± 0.041	0.627HisGln: 0.627 ± 0.031	1.215HisArg: 1.215 ± 0.039	0.854HisSer: 0.854 ± 0.028	0.756HisThr: 0.756 ± 0.029	1.071HisVal: 1.071 ± 0.037	0.178HisTrp: 0.178 ± 0.014	0.648HisTyr: 0.648 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.529IleAla: 5.529 ± 0.081	0.834IleCys: 0.834 ± 0.029	3.129IleAsp: 3.129 ± 0.068	4.115IleGlu: 4.115 ± 0.08	2.433IlePhe: 2.433 ± 0.053	4.481IleGly: 4.481 ± 0.075	0.981IleHis: 0.981 ± 0.033	4.424IleIle: 4.424 ± 0.086	3.859IleLys: 3.859 ± 0.072	5.816IleLeu: 5.816 ± 0.089	1.537IleMet: 1.537 ± 0.042	2.497IleAsn: 2.497 ± 0.055	3.056IlePro: 3.056 ± 0.063	1.636IleGln: 1.636 ± 0.044	3.606IleArg: 3.606 ± 0.068	3.761IleSer: 3.761 ± 0.067	3.461IleThr: 3.461 ± 0.078	4.038IleVal: 4.038 ± 0.066	0.527IleTrp: 0.527 ± 0.024	1.926IleTyr: 1.926 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	5.103LysAla: 5.103 ± 0.079	0.544LysCys: 0.544 ± 0.028	2.74LysAsp: 2.74 ± 0.062	5.024LysGlu: 5.024 ± 0.096	1.711LysPhe: 1.711 ± 0.043	4.022LysGly: 4.022 ± 0.071	0.898LysHis: 0.898 ± 0.035	4.15LysIle: 4.15 ± 0.066	4.467LysLys: 4.467 ± 0.084	4.769LysLeu: 4.769 ± 0.077	1.549LysMet: 1.549 ± 0.035	2.379LysAsn: 2.379 ± 0.058	2.33LysPro: 2.33 ± 0.062	1.571LysGln: 1.571 ± 0.044	3.065LysArg: 3.065 ± 0.063	2.553LysSer: 2.553 ± 0.062	2.766LysThr: 2.766 ± 0.065	4.428LysVal: 4.428 ± 0.072	0.51LysTrp: 0.51 ± 0.028	1.784LysTyr: 1.784 ± 0.047	0.0LysXaa: 0.0 ± 0.0
Leu	11.632LeuAla: 11.632 ± 0.14	1.113LeuCys: 1.113 ± 0.04	5.101LeuAsp: 5.101 ± 0.08	7.558LeuGlu: 7.558 ± 0.121	3.836LeuPhe: 3.836 ± 0.069	7.512LeuGly: 7.512 ± 0.09	1.62LeuHis: 1.62 ± 0.044	5.697LeuIle: 5.697 ± 0.084	6.861LeuLys: 6.861 ± 0.111	10.224LeuLeu: 10.224 ± 0.151	2.136LeuMet: 2.136 ± 0.051	3.668LeuAsn: 3.668 ± 0.068	5.19LeuPro: 5.19 ± 0.087	2.97LeuGln: 2.97 ± 0.069	5.86LeuArg: 5.86 ± 0.083	5.937LeuSer: 5.937 ± 0.098	4.92LeuThr: 4.92 ± 0.076	7.437LeuVal: 7.437 ± 0.097	0.871LeuTrp: 0.871 ± 0.034	2.793LeuTyr: 2.793 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.784MetAla: 2.784 ± 0.062	0.211MetCys: 0.211 ± 0.017	1.159MetAsp: 1.159 ± 0.035	1.822MetGlu: 1.822 ± 0.053	0.757MetPhe: 0.757 ± 0.03	1.938MetGly: 1.938 ± 0.053	0.378MetHis: 0.378 ± 0.019	1.328MetIle: 1.328 ± 0.038	1.48MetLys: 1.48 ± 0.043	2.367MetLeu: 2.367 ± 0.048	0.542MetMet: 0.542 ± 0.029	0.827MetAsn: 0.827 ± 0.032	1.278MetPro: 1.278 ± 0.043	0.723MetGln: 0.723 ± 0.028	1.299MetArg: 1.299 ± 0.045	1.284MetSer: 1.284 ± 0.043	1.126MetThr: 1.126 ± 0.039	1.875MetVal: 1.875 ± 0.041	0.144MetTrp: 0.144 ± 0.013	0.535MetTyr: 0.535 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	2.482AsnAla: 2.482 ± 0.06	0.513AsnCys: 0.513 ± 0.024	1.18AsnAsp: 1.18 ± 0.041	1.925AsnGlu: 1.925 ± 0.051	1.388AsnPhe: 1.388 ± 0.038	2.696AsnGly: 2.696 ± 0.063	0.506AsnHis: 0.506 ± 0.026	2.553AsnIle: 2.553 ± 0.058	1.799AsnLys: 1.799 ± 0.049	3.706AsnLeu: 3.706 ± 0.062	0.854AsnMet: 0.854 ± 0.035	1.258AsnAsn: 1.258 ± 0.043	2.103AsnPro: 2.103 ± 0.056	0.919AsnGln: 0.919 ± 0.038	2.259AsnArg: 2.259 ± 0.057	1.73AsnSer: 1.73 ± 0.043	1.531AsnThr: 1.531 ± 0.045	2.182AsnVal: 2.182 ± 0.053	0.43AsnTrp: 0.43 ± 0.024	1.17AsnTyr: 1.17 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	5.209ProAla: 5.209 ± 0.103	0.521ProCys: 0.521 ± 0.027	2.709ProAsp: 2.709 ± 0.061	4.281ProGlu: 4.281 ± 0.066	1.878ProPhe: 1.878 ± 0.046	5.192ProGly: 5.192 ± 0.089	0.795ProHis: 0.795 ± 0.033	1.533ProIle: 1.533 ± 0.039	1.652ProLys: 1.652 ± 0.05	4.257ProLeu: 4.257 ± 0.071	0.758ProMet: 0.758 ± 0.031	1.082ProAsn: 1.082 ± 0.035	2.182ProPro: 2.182 ± 0.073	1.354ProGln: 1.354 ± 0.048	2.389ProArg: 2.389 ± 0.056	2.078ProSer: 2.078 ± 0.048	1.507ProThr: 1.507 ± 0.043	4.802ProVal: 4.802 ± 0.076	0.496ProTrp: 0.496 ± 0.026	1.419ProTyr: 1.419 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	3.312GlnAla: 3.312 ± 0.067	0.239GlnCys: 0.239 ± 0.015	1.355GlnAsp: 1.355 ± 0.041	2.41GlnGlu: 2.41 ± 0.078	1.054GlnPhe: 1.054 ± 0.037	2.17GlnGly: 2.17 ± 0.053	0.534GlnHis: 0.534 ± 0.027	1.84GlnIle: 1.84 ± 0.046	2.122GlnLys: 2.122 ± 0.053	2.717GlnLeu: 2.717 ± 0.068	0.814GlnMet: 0.814 ± 0.034	1.036GlnAsn: 1.036 ± 0.035	1.32GlnPro: 1.32 ± 0.043	1.014GlnGln: 1.014 ± 0.041	1.624GlnArg: 1.624 ± 0.042	1.312GlnSer: 1.312 ± 0.037	1.285GlnThr: 1.285 ± 0.042	2.778GlnVal: 2.778 ± 0.063	0.262GlnTrp: 0.262 ± 0.017	0.842GlnTyr: 0.842 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	5.251ArgAla: 5.251 ± 0.075	0.786ArgCys: 0.786 ± 0.033	2.807ArgAsp: 2.807 ± 0.051	5.814ArgGlu: 5.814 ± 0.093	2.529ArgPhe: 2.529 ± 0.062	4.376ArgGly: 4.376 ± 0.069	1.25ArgHis: 1.25 ± 0.04	3.831ArgIle: 3.831 ± 0.073	3.538ArgLys: 3.538 ± 0.069	7.183ArgLeu: 7.183 ± 0.115	1.626ArgMet: 1.626 ± 0.049	2.036ArgAsn: 2.036 ± 0.05	3.135ArgPro: 3.135 ± 0.064	2.761ArgGln: 2.761 ± 0.082	4.823ArgArg: 4.823 ± 0.093	2.761ArgSer: 2.761 ± 0.057	2.702ArgThr: 2.702 ± 0.05	4.837ArgVal: 4.837 ± 0.079	0.721ArgTrp: 0.721 ± 0.028	2.089ArgTyr: 2.089 ± 0.056	0.0ArgXaa: 0.0 ± 0.0
Ser	4.168SerAla: 4.168 ± 0.082	0.728SerCys: 0.728 ± 0.029	2.046SerAsp: 2.046 ± 0.057	2.938SerGlu: 2.938 ± 0.056	2.378SerPhe: 2.378 ± 0.051	5.029SerGly: 5.029 ± 0.088	0.869SerHis: 0.869 ± 0.032	3.005SerIle: 3.005 ± 0.06	2.239SerLys: 2.239 ± 0.046	5.759SerLeu: 5.759 ± 0.099	1.234SerMet: 1.234 ± 0.034	1.433SerAsn: 1.433 ± 0.044	2.628SerPro: 2.628 ± 0.055	1.413SerGln: 1.413 ± 0.039	3.604SerArg: 3.604 ± 0.071	2.798SerSer: 2.798 ± 0.073	2.266SerThr: 2.266 ± 0.047	3.574SerVal: 3.574 ± 0.069	0.531SerTrp: 0.531 ± 0.03	1.564SerTyr: 1.564 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.755ThrAla: 4.755 ± 0.081	0.632ThrCys: 0.632 ± 0.025	1.986ThrAsp: 1.986 ± 0.046	2.5ThrGlu: 2.5 ± 0.059	1.701ThrPhe: 1.701 ± 0.048	5.659ThrGly: 5.659 ± 0.083	0.76ThrHis: 0.76 ± 0.03	2.539ThrIle: 2.539 ± 0.053	1.605ThrLys: 1.605 ± 0.044	4.253ThrLeu: 4.253 ± 0.078	0.987ThrMet: 0.987 ± 0.04	1.221ThrAsn: 1.221 ± 0.042	2.391ThrPro: 2.391 ± 0.057	0.919ThrGln: 0.919 ± 0.033	2.662ThrArg: 2.662 ± 0.054	2.14ThrSer: 2.14 ± 0.054	2.078ThrThr: 2.078 ± 0.061	4.564ThrVal: 4.564 ± 0.081	0.477ThrTrp: 0.477 ± 0.03	1.251ThrTyr: 1.251 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	7.068ValAla: 7.068 ± 0.09	0.955ValCys: 0.955 ± 0.038	3.842ValAsp: 3.842 ± 0.063	5.599ValGlu: 5.599 ± 0.096	3.176ValPhe: 3.176 ± 0.069	4.9ValGly: 4.9 ± 0.092	1.343ValHis: 1.343 ± 0.041	5.262ValIle: 5.262 ± 0.086	4.535ValLys: 4.535 ± 0.066	8.422ValLeu: 8.422 ± 0.107	1.891ValMet: 1.891 ± 0.05	2.836ValAsn: 2.836 ± 0.063	3.755ValPro: 3.755 ± 0.072	2.207ValGln: 2.207 ± 0.056	4.794ValArg: 4.794 ± 0.082	4.264ValSer: 4.264 ± 0.077	3.854ValThr: 3.854 ± 0.07	6.328ValVal: 6.328 ± 0.109	0.64ValTrp: 0.64 ± 0.031	2.351ValTyr: 2.351 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.79TrpAla: 0.79 ± 0.032	0.111TrpCys: 0.111 ± 0.011	0.556TrpAsp: 0.556 ± 0.025	0.736TrpGlu: 0.736 ± 0.031	0.335TrpPhe: 0.335 ± 0.019	0.783TrpGly: 0.783 ± 0.035	0.222TrpHis: 0.222 ± 0.016	0.454TrpIle: 0.454 ± 0.025	0.505TrpLys: 0.505 ± 0.03	1.235TrpLeu: 1.235 ± 0.045	0.21TrpMet: 0.21 ± 0.015	0.349TrpAsn: 0.349 ± 0.021	0.464TrpPro: 0.464 ± 0.025	0.485TrpGln: 0.485 ± 0.024	0.656TrpArg: 0.656 ± 0.031	0.488TrpSer: 0.488 ± 0.028	0.388TrpThr: 0.388 ± 0.022	0.616TrpVal: 0.616 ± 0.028	0.147TrpTrp: 0.147 ± 0.014	0.303TrpTyr: 0.303 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.3TyrAla: 2.3 ± 0.053	0.485TyrCys: 0.485 ± 0.024	1.486TyrAsp: 1.486 ± 0.042	1.781TyrGlu: 1.781 ± 0.044	1.385TyrPhe: 1.385 ± 0.044	2.72TyrGly: 2.72 ± 0.061	0.653TyrHis: 0.653 ± 0.028	1.862TyrIle: 1.862 ± 0.05	1.412TyrLys: 1.412 ± 0.039	3.433TyrLeu: 3.433 ± 0.066	0.547TyrMet: 0.547 ± 0.025	1.145TyrAsn: 1.145 ± 0.04	1.51TyrPro: 1.51 ± 0.04	0.994TyrGln: 0.994 ± 0.032	2.567TyrArg: 2.567 ± 0.056	1.639TyrSer: 1.639 ± 0.046	1.467TyrThr: 1.467 ± 0.041	1.865TyrVal: 1.865 ± 0.046	0.327TyrTrp: 0.327 ± 0.022	1.16TyrTyr: 1.16 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2884 proteins (856099 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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