Mycobacterium phage SiSi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4JH80|R4JH80_9CAUD Uncharacterized protein OS=Mycobacterium phage SiSi OX=1327777 GN=78 PE=4 SV=1
MM1 pKa = 7.01THH3 pKa = 6.73HH4 pKa = 7.63PDD6 pKa = 3.69EE7 pKa = 5.31IDD9 pKa = 4.62PISQLEE15 pKa = 4.03LDD17 pKa = 3.73ILANSGDD24 pKa = 4.19LDD26 pKa = 4.2LATKK30 pKa = 10.37AGEE33 pKa = 4.25VAQWAYY39 pKa = 9.17VQSVEE44 pKa = 4.44ALCALLCLPPEE55 pKa = 4.39VAEE58 pKa = 4.0QMVNEE63 pKa = 4.5WFLKK67 pKa = 10.29

Molecular weight: 7.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4JFR7|R4JFR7_9CAUD Holin OS=Mycobacterium phage SiSi OX=1327777 GN=32 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.18IHH4 pKa = 5.44VQSRR8 pKa = 11.84GPAGWNATVLFTAGTVYY25 pKa = 10.11TVADD29 pKa = 3.79DD30 pKa = 3.38QGRR33 pKa = 11.84KK34 pKa = 9.22HH35 pKa = 6.73LIDD38 pKa = 3.4TSRR41 pKa = 11.84VTVRR45 pKa = 11.84RR46 pKa = 11.84LSS48 pKa = 3.31

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

17977

25

1160

181.6

19.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.558 ± 0.456

1.274 ± 0.18

6.714 ± 0.217

5.952 ± 0.343

3.043 ± 0.208

8.795 ± 0.582

2.309 ± 0.208

4.35 ± 0.181

3.382 ± 0.195

7.326 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.117

3.377 ± 0.197

6.113 ± 0.208

3.382 ± 0.194

6.825 ± 0.387

5.763 ± 0.289

6.692 ± 0.233

7.048 ± 0.297

2.309 ± 0.153

2.498 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski