Acidilobus sp. SCGC AC-742_E15

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Acidilobales; Acidilobaceae; Acidilobus; unclassified Acidilobus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 672 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9WZU2|A0A2T9WZU2_9CREN Site-specific DNA-methyltransferase (adenine-specific) (Fragment) OS=Acidilobus sp. SCGC AC-742_E15 OX=1987488 GN=DDW07_01715 PE=4 SV=1
WW1 pKa = 7.23YY2 pKa = 8.92LAPHH6 pKa = 6.22VIKK9 pKa = 10.11WYY11 pKa = 10.1PNDD14 pKa = 3.42TGMAPISLAIALAQPSNVSGNAAITITITVASAYY48 pKa = 10.49VNQFTINGLMIAGAAIDD65 pKa = 4.19AVVDD69 pKa = 3.99DD70 pKa = 5.04VEE72 pKa = 5.13VGDD75 pKa = 4.42LFQAISQLFPQLGTTSTFTTEE96 pKa = 3.9GKK98 pKa = 9.56VAEE101 pKa = 4.52GNGVVVEE108 pKa = 5.19TITSTIIPGTADD120 pKa = 2.67IGQLYY125 pKa = 10.19IVGQVALVNWTAYY138 pKa = 8.27MLPLVSNTPVAWYY151 pKa = 10.43LSLQLTEE158 pKa = 5.53FKK160 pKa = 10.84AVEE163 pKa = 4.0NSGAVAPVIYY173 pKa = 9.69HH174 pKa = 5.43WWAYY178 pKa = 9.96DD179 pKa = 3.15PCINVSEE186 pKa = 4.33WAYY189 pKa = 8.85EE190 pKa = 4.03QQVLMQSPSYY200 pKa = 10.84FFGTGMNSTCPLPNTPAPVIWQDD223 pKa = 3.2YY224 pKa = 7.63ATNLTYY230 pKa = 9.2FTTKK234 pKa = 10.48NPNSQQQYY242 pKa = 8.16SYY244 pKa = 11.97VDD246 pKa = 3.65FFVNGGEE253 pKa = 4.13TLLGIGIDD261 pKa = 3.52AGSAIASIIGTVLMASQPEE280 pKa = 3.65IGAAVEE286 pKa = 4.61LIGALLDD293 pKa = 3.53SFQYY297 pKa = 10.52QSYY300 pKa = 10.18SVAVTVNNFLVSNTFPRR317 pKa = 11.84GYY319 pKa = 9.72YY320 pKa = 9.09VNVYY324 pKa = 9.54FSNLTPMYY332 pKa = 9.04TVSDD336 pKa = 3.45QGFTLPNEE344 pKa = 4.82LILINVTSS352 pKa = 3.61

Molecular weight: 38.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9X1A0|A0A2T9X1A0_9CREN Uncharacterized protein OS=Acidilobus sp. SCGC AC-742_E15 OX=1987488 GN=DDW07_01025 PE=4 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84HH4 pKa = 5.71KK5 pKa = 10.51PLARR9 pKa = 11.84KK10 pKa = 9.13LRR12 pKa = 11.84LARR15 pKa = 11.84ASKK18 pKa = 10.49SSQPVPAWVIVKK30 pKa = 7.32TLRR33 pKa = 11.84KK34 pKa = 8.6FTFNPKK40 pKa = 8.36RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.36WRR45 pKa = 11.84STKK48 pKa = 10.73LKK50 pKa = 9.86VV51 pKa = 3.04

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

672

0

672

177325

32

1262

263.9

29.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.436 ± 0.105

0.658 ± 0.034

4.46 ± 0.079

6.937 ± 0.113

3.341 ± 0.057

8.034 ± 0.083

1.39 ± 0.035

5.282 ± 0.077

4.388 ± 0.11

11.154 ± 0.113

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.043

2.619 ± 0.078

5.154 ± 0.083

2.15 ± 0.05

7.025 ± 0.142

6.627 ± 0.091

4.586 ± 0.101

9.287 ± 0.097

1.259 ± 0.045

3.864 ± 0.077
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski