Odonata-associated circular virus-15
Average proteome isoelectric point is 7.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UGI9|A0A0B4UGI9_9VIRU Replication-associated protein OS=Odonata-associated circular virus-15 OX=1592115 PE=3 SV=1
MM1 pKa = 7.5 SSNASSLSTSTNSFRR16 pKa = 11.84 IQAKK20 pKa = 10.19 SFFLTYY26 pKa = 8.6 PQCSASKK33 pKa = 10.28 QDD35 pKa = 4.34 LKK37 pKa = 11.56 NFLDD41 pKa = 3.65 TKK43 pKa = 10.89 GRR45 pKa = 11.84 IVYY48 pKa = 8.78 YY49 pKa = 10.67 LIGQEE54 pKa = 3.78 RR55 pKa = 11.84 HH56 pKa = 5.46 EE57 pKa = 5.39 DD58 pKa = 3.95 GNFHH62 pKa = 6.64 LHH64 pKa = 6.92 ALVTYY69 pKa = 8.17 EE70 pKa = 4.34 KK71 pKa = 10.17 KK72 pKa = 10.68 INVKK76 pKa = 9.02 RR77 pKa = 11.84 TTFFDD82 pKa = 4.09 LNGFHH87 pKa = 7.47 PNIQAAKK94 pKa = 10.06 NLPALKK100 pKa = 10.42 NYY102 pKa = 9.52 ISKK105 pKa = 10.79 EE106 pKa = 3.99 DD107 pKa = 3.86 IEE109 pKa = 4.76 PLTSTVEE116 pKa = 4.04 PEE118 pKa = 4.29 EE119 pKa = 4.05 EE120 pKa = 4.11 DD121 pKa = 3.5 NLYY124 pKa = 11.11 DD125 pKa = 3.99 LARR128 pKa = 11.84 VTPEE132 pKa = 3.37 EE133 pKa = 4.43 NYY135 pKa = 10.46 FEE137 pKa = 4.4 ICRR140 pKa = 11.84 KK141 pKa = 9.98 RR142 pKa = 11.84 KK143 pKa = 7.9 VHH145 pKa = 6.14 PSIISRR151 pKa = 11.84 VPFMYY156 pKa = 10.63 ANQAFLKK163 pKa = 9.44 ILKK166 pKa = 9.34 DD167 pKa = 3.44 ASVNTIGEE175 pKa = 4.43 EE176 pKa = 4.07 YY177 pKa = 10.45 QVIGTITSTVLQNLQLPTDD196 pKa = 3.87 MTSLWVKK203 pKa = 10.19 GPSGIGKK210 pKa = 5.86 TTWALTVSNKK220 pKa = 9.2 PALFVRR226 pKa = 11.84 HH227 pKa = 6.8 LDD229 pKa = 3.36 TLRR232 pKa = 11.84 EE233 pKa = 3.97 FRR235 pKa = 11.84 NGYY238 pKa = 9.51 HH239 pKa = 5.6 KK240 pKa = 9.59 TIIFDD245 pKa = 3.81 DD246 pKa = 3.89 MSFQHH251 pKa = 6.94 LPRR254 pKa = 11.84 TAQIEE259 pKa = 4.38 LVDD262 pKa = 4.61 RR263 pKa = 11.84 YY264 pKa = 10.24 HH265 pKa = 6.83 PQQIHH270 pKa = 4.91 IRR272 pKa = 11.84 YY273 pKa = 8.58 AVVNIPPNIPKK284 pKa = 9.92 IFLSNDD290 pKa = 3.41 SIFTYY295 pKa = 10.67 DD296 pKa = 3.03 EE297 pKa = 4.85 AIFRR301 pKa = 11.84 RR302 pKa = 11.84 LTLVNLEE309 pKa = 4.36 FTDD312 pKa = 3.61 QQ313 pKa = 3.28
Molecular weight: 36.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.911
IPC2_protein 7.102
IPC_protein 7.366
Toseland 7.439
ProMoST 7.541
Dawson 7.951
Bjellqvist 7.775
Wikipedia 7.922
Rodwell 7.922
Grimsley 7.512
Solomon 8.141
Lehninger 8.141
Nozaki 7.995
DTASelect 8.009
Thurlkill 8.053
EMBOSS 8.214
Sillero 8.229
Patrickios 4.38
IPC_peptide 8.141
IPC2_peptide 7.249
IPC2.peptide.svr19 7.436
Protein with the highest isoelectric point:
>tr|A0A0B4UGI9|A0A0B4UGI9_9VIRU Replication-associated protein OS=Odonata-associated circular virus-15 OX=1592115 PE=3 SV=1
MM1 pKa = 7.67 IPLQMGYY8 pKa = 11.45 SMVRR12 pKa = 11.84 RR13 pKa = 11.84 NARR16 pKa = 11.84 AIGRR20 pKa = 11.84 YY21 pKa = 9.25 SGGAKK26 pKa = 9.0 MAWRR30 pKa = 11.84 LGQYY34 pKa = 10.05 AGRR37 pKa = 11.84 ALRR40 pKa = 11.84 RR41 pKa = 11.84 FRR43 pKa = 11.84 RR44 pKa = 11.84 GGGGGGSSSSAKK56 pKa = 10.2 AKK58 pKa = 10.62 AVGSLSEE65 pKa = 4.09 QRR67 pKa = 11.84 DD68 pKa = 3.44 VTNLYY73 pKa = 9.61 RR74 pKa = 11.84 RR75 pKa = 11.84 KK76 pKa = 9.2 RR77 pKa = 11.84 APRR80 pKa = 11.84 RR81 pKa = 11.84 VRR83 pKa = 11.84 RR84 pKa = 11.84 AARR87 pKa = 11.84 KK88 pKa = 5.34 FTKK91 pKa = 10.23 RR92 pKa = 11.84 FMYY95 pKa = 10.25 TLDD98 pKa = 3.2 KK99 pKa = 8.79 TQGMKK104 pKa = 9.3 TCVITNAAQFSGSPTGLANGQQTIGITMYY133 pKa = 10.78 GYY135 pKa = 8.0 NTNSYY140 pKa = 10.68 ASNIDD145 pKa = 3.68 LGNGDD150 pKa = 4.36 MPWIFARR157 pKa = 11.84 EE158 pKa = 3.69 NGAYY162 pKa = 6.85 PTAASGTRR170 pKa = 11.84 YY171 pKa = 9.95 LRR173 pKa = 11.84 FRR175 pKa = 11.84 SCTMNYY181 pKa = 10.09 SIQNTWEE188 pKa = 3.66 QGVYY192 pKa = 8.93 MDD194 pKa = 4.55 IYY196 pKa = 10.73 FVIARR201 pKa = 11.84 KK202 pKa = 10.25 SNGSTSDD209 pKa = 4.1 PAAEE213 pKa = 4.05 WNEE216 pKa = 3.79 QIAIQNAGNMPTAITTNQYY235 pKa = 11.03 YY236 pKa = 11.11 GLTPFDD242 pKa = 3.55 APGFGRR248 pKa = 11.84 YY249 pKa = 7.41 WLIKK253 pKa = 8.15 SRR255 pKa = 11.84 KK256 pKa = 8.75 RR257 pKa = 11.84 VFIQPAEE264 pKa = 4.07 IYY266 pKa = 10.68 SFQQRR271 pKa = 11.84 DD272 pKa = 3.13 AGNYY276 pKa = 7.46 VLNMSDD282 pKa = 3.82 VLDD285 pKa = 3.92 IKK287 pKa = 10.88 LKK289 pKa = 10.21 QNLTEE294 pKa = 4.08 GVILVFHH301 pKa = 6.65 NPVTDD306 pKa = 3.42 TVTTPGTIVPGGWQVQVTCTKK327 pKa = 8.98 TYY329 pKa = 10.17 HH330 pKa = 5.99 YY331 pKa = 10.51 SEE333 pKa = 4.99 TSASTDD339 pKa = 3.5 AIGVV343 pKa = 3.73
Molecular weight: 38.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.069
IPC2_protein 9.794
IPC_protein 10.35
Toseland 10.058
ProMoST 9.926
Dawson 10.335
Bjellqvist 10.101
Wikipedia 10.584
Rodwell 10.526
Grimsley 10.452
Solomon 10.365
Lehninger 10.321
Nozaki 10.058
DTASelect 10.087
Thurlkill 10.16
EMBOSS 10.482
Sillero 10.248
Patrickios 7.541
IPC_peptide 10.365
IPC2_peptide 9.048
IPC2.peptide.svr19 8.68
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
656
313
343
328.0
37.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.165 ± 1.457
0.762 ± 0.087
4.268 ± 0.599
3.963 ± 1.269
4.726 ± 0.954
6.86 ± 2.601
1.829 ± 0.969
6.86 ± 0.8
5.03 ± 0.738
6.86 ± 1.707
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.439 ± 0.824
6.098 ± 0.207
4.421 ± 0.49
4.878 ± 0.288
6.86 ± 1.241
7.012 ± 0.012
8.384 ± 0.282
5.335 ± 0.068
1.22 ± 0.412
5.03 ± 0.623
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here