Pseudomonas phage PA1C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 401 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BJY6|A0A4D6BJY6_9CAUD Uncharacterized protein OS=Pseudomonas phage PA1C OX=2562177 PE=4 SV=1
MM1 pKa = 7.22ATQLIGAAVEE11 pKa = 4.17VRR13 pKa = 11.84TEE15 pKa = 4.2AIEE18 pKa = 3.96LFKK21 pKa = 11.51NKK23 pKa = 8.84VRR25 pKa = 11.84EE26 pKa = 4.02LSKK29 pKa = 11.39DD30 pKa = 3.42DD31 pKa = 3.96TNDD34 pKa = 3.57GNDD37 pKa = 3.78VLCGAAAAAIDD48 pKa = 4.0YY49 pKa = 10.77AVGGVLDD56 pKa = 3.88AVEE59 pKa = 4.0NHH61 pKa = 6.0YY62 pKa = 11.29EE63 pKa = 4.21DD64 pKa = 5.53IITGSTDD71 pKa = 2.9HH72 pKa = 7.71DD73 pKa = 4.05EE74 pKa = 4.66DD75 pKa = 4.76PGVNLTCVSGLSEE88 pKa = 4.45TYY90 pKa = 10.34FEE92 pKa = 4.93SVNTT96 pKa = 4.18

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BG38|A0A4D6BG38_9CAUD Uncharacterized protein OS=Pseudomonas phage PA1C OX=2562177 PE=4 SV=1
MM1 pKa = 7.33LVEE4 pKa = 4.71TIDD7 pKa = 4.26LIEE10 pKa = 4.41IVKK13 pKa = 10.53SSNKK17 pKa = 8.52KK18 pKa = 7.42TVEE21 pKa = 3.74LHH23 pKa = 5.91DD24 pKa = 5.44AIVGVLRR31 pKa = 11.84DD32 pKa = 3.85LLYY35 pKa = 8.88EE36 pKa = 4.13TSSLRR41 pKa = 11.84TRR43 pKa = 11.84LRR45 pKa = 11.84CQSKK49 pKa = 10.3RR50 pKa = 11.84EE51 pKa = 4.11APWVRR56 pKa = 11.84KK57 pKa = 9.77YY58 pKa = 9.73IAKK61 pKa = 8.54RR62 pKa = 11.84TTYY65 pKa = 9.63RR66 pKa = 11.84HH67 pKa = 6.01RR68 pKa = 11.84PFHH71 pKa = 6.86LSVQRR76 pKa = 11.84EE77 pKa = 3.79NGITIRR83 pKa = 11.84GSSYY87 pKa = 7.43QRR89 pKa = 11.84RR90 pKa = 11.84YY91 pKa = 10.17IEE93 pKa = 3.99GTLPFRR99 pKa = 11.84TRR101 pKa = 11.84RR102 pKa = 11.84GYY104 pKa = 10.76

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

401

0

401

93661

30

1566

233.6

26.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.013 ± 0.159

0.912 ± 0.042

6.414 ± 0.093

6.844 ± 0.174

4.123 ± 0.066

6.465 ± 0.169

2.056 ± 0.071

6.481 ± 0.085

5.853 ± 0.127

8.399 ± 0.109

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.067

5.251 ± 0.081

4.097 ± 0.081

3.535 ± 0.084

5.264 ± 0.107

5.788 ± 0.094

6.123 ± 0.099

7.169 ± 0.097

1.368 ± 0.047

3.981 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski