Capybara microvirus Cap1_SP_259
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4M0|A0A4P8W4M0_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_259 OX=2585419 PE=4 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.48 FSFKK6 pKa = 10.15 RR7 pKa = 11.84 SKK9 pKa = 9.24 FTPHH13 pKa = 6.57 SSRR16 pKa = 11.84 IASLRR21 pKa = 11.84 AGKK24 pKa = 9.92 IYY26 pKa = 9.92 PILVRR31 pKa = 11.84 EE32 pKa = 4.55 MYY34 pKa = 10.26 PGDD37 pKa = 4.54 TITIWHH43 pKa = 5.88 QALLRR48 pKa = 11.84 TAPMLFPLYY57 pKa = 10.91 SPMKK61 pKa = 9.35 LTFDD65 pKa = 3.61 YY66 pKa = 10.04 FYY68 pKa = 11.04 SPNRR72 pKa = 11.84 IVWKK76 pKa = 10.49 GDD78 pKa = 3.2 EE79 pKa = 4.36 TKK81 pKa = 10.56 DD82 pKa = 2.89 WSAFITGSINGKK94 pKa = 10.04 KK95 pKa = 9.65 IEE97 pKa = 5.27 DD98 pKa = 3.85 PDD100 pKa = 4.09 KK101 pKa = 11.1 LPKK104 pKa = 10.21 LPYY107 pKa = 8.66 ITVPEE112 pKa = 4.55 GGFGKK117 pKa = 10.58 SSLADD122 pKa = 3.35 YY123 pKa = 11.14 LGFDD127 pKa = 3.57 PSDD130 pKa = 3.45 RR131 pKa = 11.84 LEE133 pKa = 4.17 GAKK136 pKa = 10.41 LSALRR141 pKa = 11.84 FRR143 pKa = 11.84 HH144 pKa = 4.41 YY145 pKa = 10.98 QKK147 pKa = 10.59 IISDD151 pKa = 4.01 YY152 pKa = 10.71 YY153 pKa = 11.07 ADD155 pKa = 4.4 EE156 pKa = 4.69 NLSTMPEE163 pKa = 3.46 IAYY166 pKa = 8.92 TEE168 pKa = 4.67 GEE170 pKa = 4.3 DD171 pKa = 3.64 TTTSLDD177 pKa = 3.57 LFTRR181 pKa = 11.84 CWNKK185 pKa = 10.54 DD186 pKa = 3.06 RR187 pKa = 11.84 FTTASLDD194 pKa = 3.7 PSSGEE199 pKa = 3.87 SVMIPIGNSAPVVGNGNSLGLTNGVSDD226 pKa = 4.59 FGLVSGQLYY235 pKa = 10.33 INTDD239 pKa = 3.28 RR240 pKa = 11.84 EE241 pKa = 4.61 GVLNTSQSTYY251 pKa = 11.2 ALPASGLTEE260 pKa = 3.88 RR261 pKa = 11.84 AADD264 pKa = 3.57 INNSTTALGVSKK276 pKa = 10.6 DD277 pKa = 3.59 SSKK280 pKa = 11.3 SGLVADD286 pKa = 5.23 LEE288 pKa = 4.44 AATGIGVDD296 pKa = 3.58 EE297 pKa = 4.46 LHH299 pKa = 7.22 FLVQLQRR306 pKa = 11.84 LNQQDD311 pKa = 4.34 AFWGTRR317 pKa = 11.84 DD318 pKa = 3.6 FEE320 pKa = 4.69 CIYY323 pKa = 9.6 THH325 pKa = 6.74 FGVVIPDD332 pKa = 3.44 TRR334 pKa = 11.84 LQRR337 pKa = 11.84 ATFLGRR343 pKa = 11.84 SCVDD347 pKa = 3.17 VNFSEE352 pKa = 4.81 VLQTSQSTDD361 pKa = 2.6 SSPQGNITGHH371 pKa = 6.69 ALAVDD376 pKa = 4.07 QNNPIHH382 pKa = 6.21 FNCSEE387 pKa = 3.85 HH388 pKa = 7.8 GYY390 pKa = 10.3 IFVLVNIQPIAQYY403 pKa = 10.31 CQGIPRR409 pKa = 11.84 DD410 pKa = 3.2 AMYY413 pKa = 10.61 RR414 pKa = 11.84 DD415 pKa = 4.28 RR416 pKa = 11.84 YY417 pKa = 10.44 SYY419 pKa = 8.69 MWPEE423 pKa = 4.0 FSEE426 pKa = 4.25 TGQQEE431 pKa = 4.21 IYY433 pKa = 10.41 SAEE436 pKa = 4.13 LYY438 pKa = 8.24 ATRR441 pKa = 11.84 QNIDD445 pKa = 2.76 EE446 pKa = 4.36 RR447 pKa = 11.84 KK448 pKa = 8.91 IFGYY452 pKa = 9.14 EE453 pKa = 3.34 PRR455 pKa = 11.84 FNHH458 pKa = 6.46 LRR460 pKa = 11.84 FGDD463 pKa = 3.86 KK464 pKa = 9.44 TVHH467 pKa = 6.85 GDD469 pKa = 3.57 FRR471 pKa = 11.84 DD472 pKa = 3.81 NLSGYY477 pKa = 7.79 HH478 pKa = 4.92 TARR481 pKa = 11.84 IFSEE485 pKa = 4.26 EE486 pKa = 4.16 PEE488 pKa = 4.18 LNEE491 pKa = 3.62 NFIRR495 pKa = 11.84 ADD497 pKa = 3.06 VTQRR501 pKa = 11.84 MFAVNTQDD509 pKa = 4.01 IDD511 pKa = 4.03 QYY513 pKa = 8.24 WLRR516 pKa = 11.84 VEE518 pKa = 4.67 FDD520 pKa = 3.14 INHH523 pKa = 5.22 VRR525 pKa = 11.84 RR526 pKa = 11.84 LPKK529 pKa = 9.82 WPTPSAIGKK538 pKa = 8.66 LFF540 pKa = 3.56
Molecular weight: 61.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.335
IPC2_protein 5.347
IPC_protein 5.347
Toseland 5.537
ProMoST 5.588
Dawson 5.474
Bjellqvist 5.537
Wikipedia 5.41
Rodwell 5.41
Grimsley 5.601
Solomon 5.474
Lehninger 5.448
Nozaki 5.664
DTASelect 5.855
Thurlkill 5.664
EMBOSS 5.639
Sillero 5.741
Patrickios 3.986
IPC_peptide 5.486
IPC2_peptide 5.741
IPC2.peptide.svr19 5.723
Protein with the highest isoelectric point:
>tr|A0A4P8W881|A0A4P8W881_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_259 OX=2585419 PE=4 SV=1
MM1 pKa = 7.54 NISSFLSTRR10 pKa = 11.84 KK11 pKa = 9.86 AFRR14 pKa = 11.84 EE15 pKa = 4.04 FKK17 pKa = 10.83 DD18 pKa = 3.47 FGVFLDD24 pKa = 3.44 VDD26 pKa = 3.61 KK27 pKa = 11.45 KK28 pKa = 10.48 NRR30 pKa = 11.84 CRR32 pKa = 11.84 LMLRR36 pKa = 11.84 HH37 pKa = 6.14 IPSGRR42 pKa = 11.84 VIEE45 pKa = 4.06 LRR47 pKa = 11.84 KK48 pKa = 9.47 FVNVQSYY55 pKa = 7.83 QRR57 pKa = 11.84 FLVQGVFLRR66 pKa = 11.84 LIADD70 pKa = 3.59 SASVNKK76 pKa = 10.27 ANHH79 pKa = 6.21 SYY81 pKa = 11.25 INVCNTLEE89 pKa = 3.96 DD90 pKa = 4.74 LIIKK94 pKa = 9.21 EE95 pKa = 4.36 SQQ97 pKa = 2.76
Molecular weight: 11.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.143
IPC2_protein 9.194
IPC_protein 9.268
Toseland 10.16
ProMoST 9.721
Dawson 10.292
Bjellqvist 9.926
Wikipedia 10.409
Rodwell 10.745
Grimsley 10.321
Solomon 10.335
Lehninger 10.321
Nozaki 10.175
DTASelect 9.911
Thurlkill 10.16
EMBOSS 10.54
Sillero 10.204
Patrickios 10.526
IPC_peptide 10.335
IPC2_peptide 8.682
IPC2.peptide.svr19 8.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1277
97
540
255.4
28.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.987 ± 2.329
1.41 ± 0.696
6.813 ± 0.53
4.777 ± 0.844
5.873 ± 0.815
6.265 ± 0.836
2.271 ± 0.304
5.012 ± 0.858
5.717 ± 0.997
8.771 ± 0.38
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.41 ± 0.223
5.951 ± 1.054
3.289 ± 1.052
3.994 ± 0.662
6.108 ± 0.95
8.457 ± 1.064
5.247 ± 1.007
5.09 ± 0.77
1.018 ± 0.313
4.542 ± 1.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here