Farmington virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4V3J8|S4V3J8_9RHAB G OS=Farmington virus OX=1027468 PE=4 SV=1
MM1 pKa = 7.54ARR3 pKa = 11.84PLAAAQHH10 pKa = 7.07LITEE14 pKa = 4.68RR15 pKa = 11.84HH16 pKa = 5.55SLQATLSRR24 pKa = 11.84ASKK27 pKa = 8.78TRR29 pKa = 11.84AEE31 pKa = 4.17EE32 pKa = 4.0FVKK35 pKa = 10.58DD36 pKa = 4.69FYY38 pKa = 11.5LQEE41 pKa = 4.18QYY43 pKa = 11.08SVPTIPTDD51 pKa = 5.29DD52 pKa = 3.74IAQSGPMLLQAILSEE67 pKa = 4.88EE68 pKa = 4.17YY69 pKa = 10.42TKK71 pKa = 10.65ATDD74 pKa = 3.2IAQSILWNTPTPNGLLRR91 pKa = 11.84EE92 pKa = 4.31HH93 pKa = 7.2LDD95 pKa = 3.42ADD97 pKa = 3.96GGGSFTALPASAIRR111 pKa = 11.84PSDD114 pKa = 3.61EE115 pKa = 3.75ANAWAARR122 pKa = 11.84ISDD125 pKa = 3.88SGLGPVFYY133 pKa = 10.3AALAAYY139 pKa = 9.47IIGWSGRR146 pKa = 11.84GEE148 pKa = 4.13TSRR151 pKa = 11.84VQQNIGQKK159 pKa = 9.17WLMNLNAIFGTTITHH174 pKa = 6.28PTTVRR179 pKa = 11.84LPINVVNNSLAVRR192 pKa = 11.84NGLAATLWLYY202 pKa = 10.6YY203 pKa = 10.01RR204 pKa = 11.84SSPQSQDD211 pKa = 2.35AFFYY215 pKa = 11.12GLIRR219 pKa = 11.84PCCSGYY225 pKa = 10.77LGLLHH230 pKa = 7.14RR231 pKa = 11.84VQEE234 pKa = 4.18IDD236 pKa = 3.34EE237 pKa = 4.46MEE239 pKa = 4.95PDD241 pKa = 3.94FLSDD245 pKa = 3.37PRR247 pKa = 11.84IIQVNEE253 pKa = 3.86VYY255 pKa = 10.43SALRR259 pKa = 11.84ALVQLGNDD267 pKa = 3.84FKK269 pKa = 10.86TADD272 pKa = 4.3DD273 pKa = 4.35EE274 pKa = 4.53PMQVWACRR282 pKa = 11.84GINNGYY288 pKa = 8.32LTYY291 pKa = 10.78LSEE294 pKa = 4.37TPAKK298 pKa = 9.89KK299 pKa = 10.3GAVVLMFAQCMLKK312 pKa = 10.4GDD314 pKa = 3.92SEE316 pKa = 4.44AWNSYY321 pKa = 7.6RR322 pKa = 11.84TATWVMPYY330 pKa = 9.85CDD332 pKa = 4.06NVALGAMAGYY342 pKa = 9.34IQARR346 pKa = 11.84QNTRR350 pKa = 11.84AYY352 pKa = 9.32EE353 pKa = 4.11VSAQTGLDD361 pKa = 3.36VNMAAVKK368 pKa = 10.53DD369 pKa = 4.85FEE371 pKa = 5.27ASSKK375 pKa = 10.49PKK377 pKa = 10.05AAPISLIPRR386 pKa = 11.84PADD389 pKa = 3.24VASRR393 pKa = 11.84TSEE396 pKa = 3.79RR397 pKa = 11.84PSIPEE402 pKa = 3.51VDD404 pKa = 3.48SDD406 pKa = 4.15EE407 pKa = 4.54EE408 pKa = 4.32LGGMM412 pKa = 4.8

Molecular weight: 45.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4V2M6|S4V2M6_9RHAB Large structural protein OS=Farmington virus OX=1027468 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84RR3 pKa = 11.84FFLGEE8 pKa = 3.63SSAPARR14 pKa = 11.84DD15 pKa = 3.81WEE17 pKa = 4.57SEE19 pKa = 4.09RR20 pKa = 11.84PPPYY24 pKa = 10.15AVEE27 pKa = 4.49VPQSHH32 pKa = 7.24GIRR35 pKa = 11.84VTGYY39 pKa = 6.89FQCNEE44 pKa = 3.81RR45 pKa = 11.84PKK47 pKa = 10.76SKK49 pKa = 9.17KK50 pKa = 7.63TLHH53 pKa = 5.97SFAVKK58 pKa = 10.39LCDD61 pKa = 4.12AIKK64 pKa = 10.04PVRR67 pKa = 11.84ADD69 pKa = 3.4APSLKK74 pKa = 9.97IAIWTALDD82 pKa = 3.59LAFVKK87 pKa = 10.36PPNGTVTIDD96 pKa = 3.4AAVKK100 pKa = 8.37ATPLIGNTQYY110 pKa = 11.03TVGDD114 pKa = 4.64EE115 pKa = 3.95IFQMLGRR122 pKa = 11.84RR123 pKa = 11.84GGLIVIRR130 pKa = 11.84NLPHH134 pKa = 7.51DD135 pKa = 4.05YY136 pKa = 10.54PRR138 pKa = 11.84TLIEE142 pKa = 4.13FASPEE147 pKa = 4.0PP148 pKa = 3.81

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3709

148

2129

741.8

83.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.549 ± 1.317

1.645 ± 0.27

5.473 ± 0.154

5.985 ± 0.675

3.64 ± 0.418

5.5 ± 0.485

2.346 ± 0.357

5.985 ± 0.186

4.61 ± 0.709

10.299 ± 0.477

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.615 ± 0.358

3.586 ± 0.224

6.147 ± 0.423

2.858 ± 0.49

6.713 ± 0.24

7.522 ± 0.406

6.228 ± 0.064

6.471 ± 0.397

1.699 ± 0.097

3.128 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski