Amino acid dipepetide frequency for Phormidium tenue NIES-30

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.76AlaAla: 11.76 ± 0.131
0.935AlaCys: 0.935 ± 0.027
5.707AlaAsp: 5.707 ± 0.064
6.497AlaGlu: 6.497 ± 0.075
3.219AlaPhe: 3.219 ± 0.049
6.902AlaGly: 6.902 ± 0.087
1.83AlaHis: 1.83 ± 0.037
7.068AlaIle: 7.068 ± 0.071
3.321AlaLys: 3.321 ± 0.053
12.213AlaLeu: 12.213 ± 0.11
2.338AlaMet: 2.338 ± 0.04
3.162AlaAsn: 3.162 ± 0.052
4.609AlaPro: 4.609 ± 0.072
5.381AlaGln: 5.381 ± 0.077
4.795AlaArg: 4.795 ± 0.052
5.411AlaSer: 5.411 ± 0.064
5.828AlaThr: 5.828 ± 0.071
7.33AlaVal: 7.33 ± 0.076
1.319AlaTrp: 1.319 ± 0.031
2.648AlaTyr: 2.648 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.722CysAla: 0.722 ± 0.023
0.151CysCys: 0.151 ± 0.01
0.645CysAsp: 0.645 ± 0.02
0.407CysGlu: 0.407 ± 0.017
0.371CysPhe: 0.371 ± 0.015
0.761CysGly: 0.761 ± 0.024
0.277CysHis: 0.277 ± 0.015
0.474CysIle: 0.474 ± 0.019
0.228CysLys: 0.228 ± 0.012
1.077CysLeu: 1.077 ± 0.034
0.144CysMet: 0.144 ± 0.009
0.29CysAsn: 0.29 ± 0.014
0.563CysPro: 0.563 ± 0.022
0.531CysGln: 0.531 ± 0.022
0.622CysArg: 0.622 ± 0.019
0.526CysSer: 0.526 ± 0.019
0.474CysThr: 0.474 ± 0.018
0.536CysVal: 0.536 ± 0.021
0.132CysTrp: 0.132 ± 0.009
0.288CysTyr: 0.288 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
4.831AspAla: 4.831 ± 0.061
0.585AspCys: 0.585 ± 0.025
3.061AspAsp: 3.061 ± 0.056
3.152AspGlu: 3.152 ± 0.056
2.252AspPhe: 2.252 ± 0.046
3.97AspGly: 3.97 ± 0.06
1.072AspHis: 1.072 ± 0.029
2.99AspIle: 2.99 ± 0.042
1.403AspLys: 1.403 ± 0.04
6.735AspLeu: 6.735 ± 0.088
0.96AspMet: 0.96 ± 0.025
1.549AspAsn: 1.549 ± 0.034
3.267AspPro: 3.267 ± 0.053
2.619AspGln: 2.619 ± 0.042
4.601AspArg: 4.601 ± 0.059
2.877AspSer: 2.877 ± 0.048
2.69AspThr: 2.69 ± 0.055
3.292AspVal: 3.292 ± 0.046
1.041AspTrp: 1.041 ± 0.024
1.894AspTyr: 1.894 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
7.171GluAla: 7.171 ± 0.074
0.357GluCys: 0.357 ± 0.015
2.633GluAsp: 2.633 ± 0.049
3.11GluGlu: 3.11 ± 0.06
2.132GluPhe: 2.132 ± 0.038
3.754GluGly: 3.754 ± 0.053
1.051GluHis: 1.051 ± 0.024
3.255GluIle: 3.255 ± 0.046
2.046GluLys: 2.046 ± 0.045
6.428GluLeu: 6.428 ± 0.068
1.271GluMet: 1.271 ± 0.03
1.737GluAsn: 1.737 ± 0.043
2.949GluPro: 2.949 ± 0.054
3.199GluGln: 3.199 ± 0.048
3.551GluArg: 3.551 ± 0.061
3.147GluSer: 3.147 ± 0.042
3.466GluThr: 3.466 ± 0.059
4.396GluVal: 4.396 ± 0.062
0.73GluTrp: 0.73 ± 0.022
1.328GluTyr: 1.328 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
3.292PheAla: 3.292 ± 0.047
0.506PheCys: 0.506 ± 0.019
2.321PheAsp: 2.321 ± 0.049
2.185PheGlu: 2.185 ± 0.04
1.48PhePhe: 1.48 ± 0.038
2.908PheGly: 2.908 ± 0.055
0.687PheHis: 0.687 ± 0.023
1.696PheIle: 1.696 ± 0.034
1.15PheLys: 1.15 ± 0.029
3.681PheLeu: 3.681 ± 0.059
0.643PheMet: 0.643 ± 0.022
1.338PheAsn: 1.338 ± 0.033
1.646PhePro: 1.646 ± 0.033
1.505PheGln: 1.505 ± 0.031
1.842PheArg: 1.842 ± 0.035
2.633PheSer: 2.633 ± 0.05
2.066PheThr: 2.066 ± 0.037
2.367PheVal: 2.367 ± 0.039
0.687PheTrp: 0.687 ± 0.023
1.147PheTyr: 1.147 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
6.281GlyAla: 6.281 ± 0.084
0.779GlyCys: 0.779 ± 0.024
4.363GlyAsp: 4.363 ± 0.064
4.092GlyGlu: 4.092 ± 0.066
3.08GlyPhe: 3.08 ± 0.044
5.501GlyGly: 5.501 ± 0.1
1.643GlyHis: 1.643 ± 0.04
4.226GlyIle: 4.226 ± 0.067
2.769GlyLys: 2.769 ± 0.044
8.769GlyLeu: 8.769 ± 0.105
1.688GlyMet: 1.688 ± 0.035
2.486GlyAsn: 2.486 ± 0.067
2.397GlyPro: 2.397 ± 0.037
4.094GlyGln: 4.094 ± 0.06
4.034GlyArg: 4.034 ± 0.06
4.449GlySer: 4.449 ± 0.069
4.186GlyThr: 4.186 ± 0.07
5.171GlyVal: 5.171 ± 0.072
1.345GlyTrp: 1.345 ± 0.036
2.501GlyTyr: 2.501 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.375HisAla: 1.375 ± 0.03
0.302HisCys: 0.302 ± 0.014
0.967HisAsp: 0.967 ± 0.027
0.921HisGlu: 0.921 ± 0.023
0.776HisPhe: 0.776 ± 0.021
1.437HisGly: 1.437 ± 0.033
0.714HisHis: 0.714 ± 0.022
0.985HisIle: 0.985 ± 0.025
0.496HisLys: 0.496 ± 0.018
2.603HisLeu: 2.603 ± 0.045
0.318HisMet: 0.318 ± 0.015
0.635HisAsn: 0.635 ± 0.021
1.575HisPro: 1.575 ± 0.034
1.287HisGln: 1.287 ± 0.03
1.545HisArg: 1.545 ± 0.032
1.227HisSer: 1.227 ± 0.033
0.972HisThr: 0.972 ± 0.026
0.969HisVal: 0.969 ± 0.027
0.409HisTrp: 0.409 ± 0.016
0.692HisTyr: 0.692 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.774IleAla: 6.774 ± 0.071
0.525IleCys: 0.525 ± 0.021
3.476IleAsp: 3.476 ± 0.048
3.416IleGlu: 3.416 ± 0.05
1.826IlePhe: 1.826 ± 0.037
3.836IleGly: 3.836 ± 0.048
1.118IleHis: 1.118 ± 0.027
2.099IleIle: 2.099 ± 0.04
1.644IleLys: 1.644 ± 0.035
5.004IleLeu: 5.004 ± 0.059
0.74IleMet: 0.74 ± 0.023
1.788IleAsn: 1.788 ± 0.041
2.861IlePro: 2.861 ± 0.046
2.365IleGln: 2.365 ± 0.04
2.754IleArg: 2.754 ± 0.041
3.176IleSer: 3.176 ± 0.046
3.029IleThr: 3.029 ± 0.044
3.586IleVal: 3.586 ± 0.06
0.636IleTrp: 0.636 ± 0.021
1.471IleTyr: 1.471 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
3.567LysAla: 3.567 ± 0.055
0.188LysCys: 0.188 ± 0.011
1.7LysAsp: 1.7 ± 0.041
1.644LysGlu: 1.644 ± 0.044
0.988LysPhe: 0.988 ± 0.025
2.146LysGly: 2.146 ± 0.045
0.589LysHis: 0.589 ± 0.02
1.737LysIle: 1.737 ± 0.038
1.162LysLys: 1.162 ± 0.035
3.41LysLeu: 3.41 ± 0.058
0.58LysMet: 0.58 ± 0.023
0.978LysAsn: 0.978 ± 0.029
1.818LysPro: 1.818 ± 0.041
1.724LysGln: 1.724 ± 0.037
1.94LysArg: 1.94 ± 0.042
1.758LysSer: 1.758 ± 0.037
2.165LysThr: 2.165 ± 0.04
2.131LysVal: 2.131 ± 0.046
0.304LysTrp: 0.304 ± 0.015
0.749LysTyr: 0.749 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
12.352LeuAla: 12.352 ± 0.109
1.05LeuCys: 1.05 ± 0.028
6.045LeuAsp: 6.045 ± 0.076
7.0LeuGlu: 7.0 ± 0.07
3.543LeuPhe: 3.543 ± 0.056
9.011LeuGly: 9.011 ± 0.094
2.134LeuHis: 2.134 ± 0.039
5.332LeuIle: 5.332 ± 0.061
4.268LeuLys: 4.268 ± 0.068
11.919LeuLeu: 11.919 ± 0.118
2.381LeuMet: 2.381 ± 0.043
3.711LeuAsn: 3.711 ± 0.055
6.666LeuPro: 6.666 ± 0.078
5.1LeuGln: 5.1 ± 0.072
6.796LeuArg: 6.796 ± 0.072
7.489LeuSer: 7.489 ± 0.078
6.976LeuThr: 6.976 ± 0.071
8.391LeuVal: 8.391 ± 0.09
1.713LeuTrp: 1.713 ± 0.043
2.69LeuTyr: 2.69 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
2.7MetAla: 2.7 ± 0.042
0.1MetCys: 0.1 ± 0.008
0.871MetAsp: 0.871 ± 0.03
0.988MetGlu: 0.988 ± 0.026
0.531MetPhe: 0.531 ± 0.016
1.705MetGly: 1.705 ± 0.033
0.319MetHis: 0.319 ± 0.014
0.845MetIle: 0.845 ± 0.027
0.658MetLys: 0.658 ± 0.02
2.019MetLeu: 2.019 ± 0.036
0.438MetMet: 0.438 ± 0.019
0.639MetAsn: 0.639 ± 0.019
1.236MetPro: 1.236 ± 0.029
0.934MetGln: 0.934 ± 0.027
1.024MetArg: 1.024 ± 0.026
1.088MetSer: 1.088 ± 0.025
1.454MetThr: 1.454 ± 0.031
1.786MetVal: 1.786 ± 0.038
0.149MetTrp: 0.149 ± 0.01
0.305MetTyr: 0.305 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
2.799AsnAla: 2.799 ± 0.05
0.294AsnCys: 0.294 ± 0.014
1.57AsnAsp: 1.57 ± 0.052
1.355AsnGlu: 1.355 ± 0.03
1.199AsnPhe: 1.199 ± 0.027
2.407AsnGly: 2.407 ± 0.069
0.712AsnHis: 0.712 ± 0.022
1.515AsnIle: 1.515 ± 0.036
0.72AsnLys: 0.72 ± 0.025
4.178AsnLeu: 4.178 ± 0.06
0.461AsnMet: 0.461 ± 0.019
0.996AsnAsn: 0.996 ± 0.034
2.526AsnPro: 2.526 ± 0.044
1.897AsnGln: 1.897 ± 0.038
2.21AsnArg: 2.21 ± 0.039
1.879AsnSer: 1.879 ± 0.04
1.628AsnThr: 1.628 ± 0.032
1.81AsnVal: 1.81 ± 0.042
0.52AsnTrp: 0.52 ± 0.018
0.931AsnTyr: 0.931 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
5.324ProAla: 5.324 ± 0.078
0.423ProCys: 0.423 ± 0.016
3.649ProAsp: 3.649 ± 0.065
4.08ProGlu: 4.08 ± 0.056
1.776ProPhe: 1.776 ± 0.035
3.878ProGly: 3.878 ± 0.053
1.109ProHis: 1.109 ± 0.03
2.689ProIle: 2.689 ± 0.042
1.619ProLys: 1.619 ± 0.04
5.711ProLeu: 5.711 ± 0.07
1.107ProMet: 1.107 ± 0.026
1.855ProAsn: 1.855 ± 0.036
3.063ProPro: 3.063 ± 0.063
2.708ProGln: 2.708 ± 0.043
2.375ProArg: 2.375 ± 0.04
3.388ProSer: 3.388 ± 0.058
3.413ProThr: 3.413 ± 0.049
4.047ProVal: 4.047 ± 0.055
0.862ProTrp: 0.862 ± 0.027
1.4ProTyr: 1.4 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
6.071GlnAla: 6.071 ± 0.08
0.346GlnCys: 0.346 ± 0.016
2.161GlnAsp: 2.161 ± 0.033
2.627GlnGlu: 2.627 ± 0.044
1.76GlnPhe: 1.76 ± 0.037
3.941GlnGly: 3.941 ± 0.054
1.018GlnHis: 1.018 ± 0.029
2.747GlnIle: 2.747 ± 0.038
1.517GlnLys: 1.517 ± 0.034
5.531GlnLeu: 5.531 ± 0.075
1.029GlnMet: 1.029 ± 0.03
1.425GlnAsn: 1.425 ± 0.031
3.075GlnPro: 3.075 ± 0.056
3.472GlnGln: 3.472 ± 0.075
3.765GlnArg: 3.765 ± 0.051
2.824GlnSer: 2.824 ± 0.041
3.128GlnThr: 3.128 ± 0.047
4.277GlnVal: 4.277 ± 0.051
0.784GlnTrp: 0.784 ± 0.028
1.085GlnTyr: 1.085 ± 0.028
0.0GlnXaa: 0.0 ± 0.0
Arg
4.725ArgAla: 4.725 ± 0.056
0.584ArgCys: 0.584 ± 0.019
3.172ArgAsp: 3.172 ± 0.05
3.104ArgGlu: 3.104 ± 0.045
2.371ArgPhe: 2.371 ± 0.035
3.487ArgGly: 3.487 ± 0.052
1.432ArgHis: 1.432 ± 0.032
3.095ArgIle: 3.095 ± 0.054
1.631ArgLys: 1.631 ± 0.037
7.362ArgLeu: 7.362 ± 0.073
1.207ArgMet: 1.207 ± 0.03
1.758ArgAsn: 1.758 ± 0.035
3.085ArgPro: 3.085 ± 0.047
4.07ArgGln: 4.07 ± 0.058
4.015ArgArg: 4.015 ± 0.053
3.715ArgSer: 3.715 ± 0.043
2.786ArgThr: 2.786 ± 0.051
4.112ArgVal: 4.112 ± 0.046
1.17ArgTrp: 1.17 ± 0.031
2.148ArgTyr: 2.148 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.347SerAla: 5.347 ± 0.062
0.471SerCys: 0.471 ± 0.019
3.292SerAsp: 3.292 ± 0.05
3.21SerGlu: 3.21 ± 0.051
2.237SerPhe: 2.237 ± 0.046
4.939SerGly: 4.939 ± 0.067
1.266SerHis: 1.266 ± 0.03
2.811SerIle: 2.811 ± 0.046
1.666SerLys: 1.666 ± 0.038
7.205SerLeu: 7.205 ± 0.076
1.159SerMet: 1.159 ± 0.031
1.826SerAsn: 1.826 ± 0.039
3.767SerPro: 3.767 ± 0.059
3.07SerGln: 3.07 ± 0.043
3.42SerArg: 3.42 ± 0.046
3.808SerSer: 3.808 ± 0.071
3.378SerThr: 3.378 ± 0.047
3.835SerVal: 3.835 ± 0.051
0.861SerTrp: 0.861 ± 0.024
1.561SerTyr: 1.561 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.016ThrAla: 6.016 ± 0.062
0.475ThrCys: 0.475 ± 0.018
2.882ThrAsp: 2.882 ± 0.053
2.988ThrGlu: 2.988 ± 0.039
1.969ThrPhe: 1.969 ± 0.036
4.38ThrGly: 4.38 ± 0.066
1.114ThrHis: 1.114 ± 0.028
2.911ThrIle: 2.911 ± 0.049
1.44ThrLys: 1.44 ± 0.031
7.427ThrLeu: 7.427 ± 0.079
0.948ThrMet: 0.948 ± 0.024
1.598ThrAsn: 1.598 ± 0.046
3.646ThrPro: 3.646 ± 0.052
2.775ThrGln: 2.775 ± 0.045
2.75ThrArg: 2.75 ± 0.04
3.059ThrSer: 3.059 ± 0.039
3.472ThrThr: 3.472 ± 0.061
4.835ThrVal: 4.835 ± 0.065
0.867ThrTrp: 0.867 ± 0.028
1.631ThrTyr: 1.631 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
7.621ValAla: 7.621 ± 0.084
0.664ValCys: 0.664 ± 0.021
3.982ValAsp: 3.982 ± 0.059
4.641ValGlu: 4.641 ± 0.057
2.529ValPhe: 2.529 ± 0.042
5.473ValGly: 5.473 ± 0.07
1.137ValHis: 1.137 ± 0.027
3.694ValIle: 3.694 ± 0.053
2.382ValLys: 2.382 ± 0.039
7.847ValLeu: 7.847 ± 0.086
1.631ValMet: 1.631 ± 0.036
2.483ValAsn: 2.483 ± 0.045
3.599ValPro: 3.599 ± 0.053
3.137ValGln: 3.137 ± 0.047
3.801ValArg: 3.801 ± 0.049
4.154ValSer: 4.154 ± 0.061
4.102ValThr: 4.102 ± 0.054
5.975ValVal: 5.975 ± 0.076
0.96ValTrp: 0.96 ± 0.029
1.806ValTyr: 1.806 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.276TrpAla: 1.276 ± 0.031
0.152TrpCys: 0.152 ± 0.01
0.729TrpAsp: 0.729 ± 0.028
0.764TrpGlu: 0.764 ± 0.024
0.608TrpPhe: 0.608 ± 0.024
1.109TrpGly: 1.109 ± 0.033
0.38TrpHis: 0.38 ± 0.017
0.72TrpIle: 0.72 ± 0.02
0.426TrpLys: 0.426 ± 0.022
2.163TrpLeu: 2.163 ± 0.054
0.349TrpMet: 0.349 ± 0.016
0.498TrpAsn: 0.498 ± 0.018
0.638TrpPro: 0.638 ± 0.021
1.212TrpGln: 1.212 ± 0.031
0.98TrpArg: 0.98 ± 0.028
0.856TrpSer: 0.856 ± 0.026
0.586TrpThr: 0.586 ± 0.021
1.201TrpVal: 1.201 ± 0.03
0.257TrpTrp: 0.257 ± 0.014
0.357TrpTyr: 0.357 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.268TyrAla: 2.268 ± 0.037
0.331TyrCys: 0.331 ± 0.016
1.613TyrAsp: 1.613 ± 0.036
1.529TyrGlu: 1.529 ± 0.035
1.135TyrPhe: 1.135 ± 0.031
2.309TyrGly: 2.309 ± 0.044
0.606TyrHis: 0.606 ± 0.02
1.235TyrIle: 1.235 ± 0.034
0.733TyrLys: 0.733 ± 0.026
3.285TyrLeu: 3.285 ± 0.049
0.399TyrMet: 0.399 ± 0.014
0.858TyrAsn: 0.858 ± 0.026
1.49TyrPro: 1.49 ± 0.031
1.502TyrGln: 1.502 ± 0.031
2.206TyrArg: 2.206 ± 0.046
1.667TyrSer: 1.667 ± 0.036
1.385TyrThr: 1.385 ± 0.029
1.569TyrVal: 1.569 ± 0.033
0.514TyrTrp: 0.514 ± 0.019
0.839TyrTyr: 0.839 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4999 proteins (1597586 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski