Bovine papillomavirus
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z3FWK6|A0A1Z3FWK6_9PAPI Minor capsid protein L2 OS=Bovine papillomavirus OX=10571 GN=L2 PE=3 SV=1
MM1 pKa = 7.15 SRR3 pKa = 11.84 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 9.78 RR7 pKa = 11.84 DD8 pKa = 3.18 TVQNIYY14 pKa = 8.43 NTCKK18 pKa = 10.05 IWGNCPDD25 pKa = 3.9 DD26 pKa = 4.54 VINKK30 pKa = 9.25 VEE32 pKa = 4.17 SSTIADD38 pKa = 4.25 KK39 pKa = 10.76 ILKK42 pKa = 9.26 YY43 pKa = 10.87 GSGLVYY49 pKa = 10.45 FGNAGIGTGSGGGGRR64 pKa = 11.84 LGYY67 pKa = 10.4 LPLGNRR73 pKa = 11.84 PAGRR77 pKa = 11.84 PAVPFSRR84 pKa = 11.84 PTTRR88 pKa = 11.84 VTTRR92 pKa = 11.84 VPPIVEE98 pKa = 4.14 TVVPDD103 pKa = 4.42 SIAGNVVDD111 pKa = 5.16 AGGPSVIDD119 pKa = 3.54 LTDD122 pKa = 3.42 LTTEE126 pKa = 3.96 STGVDD131 pKa = 2.9 VHH133 pKa = 6.89 PDD135 pKa = 3.34 VEE137 pKa = 4.62 PGVLEE142 pKa = 4.68 PGPTEE147 pKa = 4.07 PPSIDD152 pKa = 3.44 VTSDD156 pKa = 3.14 TSPTIQVTEE165 pKa = 4.73 SIHH168 pKa = 6.6 SNPTFEE174 pKa = 4.17 IPVVNGSTYY183 pKa = 8.57 PTEE186 pKa = 4.27 AVDD189 pKa = 3.47 INTVSTVSTIIGTEE203 pKa = 3.92 EE204 pKa = 3.92 SAPLYY209 pKa = 10.25 IPEE212 pKa = 4.56 TIEE215 pKa = 3.83 LQTFNTQRR223 pKa = 11.84 TANNTFSEE231 pKa = 4.63 TIIDD235 pKa = 3.66 DD236 pKa = 3.81 TGFDD240 pKa = 3.42 SSTPLGKK247 pKa = 10.18 RR248 pKa = 11.84 PPEE251 pKa = 4.04 GRR253 pKa = 11.84 LGGARR258 pKa = 11.84 VSVYY262 pKa = 10.32 NKK264 pKa = 9.69 RR265 pKa = 11.84 YY266 pKa = 9.38 AQVEE270 pKa = 4.32 VEE272 pKa = 4.35 NPAFIRR278 pKa = 11.84 DD279 pKa = 4.12 PYY281 pKa = 11.19 TLVQYY286 pKa = 11.39 SNVSYY291 pKa = 10.82 DD292 pKa = 3.67 PEE294 pKa = 4.15 DD295 pKa = 3.35 TVTFNRR301 pKa = 11.84 GSGDD305 pKa = 3.42 VNTAPDD311 pKa = 3.83 PAFQDD316 pKa = 3.5 VLKK319 pKa = 10.71 LSKK322 pKa = 10.25 PYY324 pKa = 7.6 TTRR327 pKa = 11.84 SPGGHH332 pKa = 5.2 VRR334 pKa = 11.84 LGRR337 pKa = 11.84 VGQRR341 pKa = 11.84 AGVKK345 pKa = 8.64 TRR347 pKa = 11.84 SGLQIGPQVHH357 pKa = 5.99 YY358 pKa = 7.16 FTRR361 pKa = 11.84 ISSIDD366 pKa = 3.33 PGEE369 pKa = 4.28 TIEE372 pKa = 5.97 LEE374 pKa = 4.34 VLGDD378 pKa = 3.88 PNTPDD383 pKa = 3.37 VLVGDD388 pKa = 4.73 DD389 pKa = 3.87 EE390 pKa = 5.04 FDD392 pKa = 3.29 IVSLTEE398 pKa = 4.0 SLADD402 pKa = 3.8 SYY404 pKa = 11.87 SEE406 pKa = 4.69 SDD408 pKa = 3.74 LLDD411 pKa = 3.45 VLEE414 pKa = 5.87 DD415 pKa = 3.12 IGEE418 pKa = 4.08 NLQLVMGGRR427 pKa = 11.84 RR428 pKa = 11.84 RR429 pKa = 11.84 GPSVPVFTPRR439 pKa = 11.84 GYY441 pKa = 10.07 PPIEE445 pKa = 4.75 SITVNFPGSGITIDD459 pKa = 3.69 EE460 pKa = 4.36 STAQSDD466 pKa = 4.01 DD467 pKa = 3.76 EE468 pKa = 4.8 VPIEE472 pKa = 4.22 PDD474 pKa = 3.03 IPSTPSTPIRR484 pKa = 11.84 PGLDD488 pKa = 3.07 VYY490 pKa = 11.6 DD491 pKa = 4.92 SIDD494 pKa = 3.81 FYY496 pKa = 11.47 LHH498 pKa = 6.65 PSLGKK503 pKa = 10.28 KK504 pKa = 9.18 LRR506 pKa = 11.84 KK507 pKa = 8.27 KK508 pKa = 10.1 RR509 pKa = 11.84 KK510 pKa = 7.99 RR511 pKa = 11.84 RR512 pKa = 11.84 FYY514 pKa = 11.37
Molecular weight: 55.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.7
IPC2_protein 4.66
IPC_protein 4.622
Toseland 4.457
ProMoST 4.736
Dawson 4.571
Bjellqvist 4.724
Wikipedia 4.469
Rodwell 4.457
Grimsley 4.368
Solomon 4.571
Lehninger 4.533
Nozaki 4.685
DTASelect 4.876
Thurlkill 4.457
EMBOSS 4.482
Sillero 4.736
Patrickios 4.024
IPC_peptide 4.584
IPC2_peptide 4.724
IPC2.peptide.svr19 4.704
Protein with the highest isoelectric point:
>tr|A0A1Z3FWF3|A0A1Z3FWF3_9PAPI Replication protein E1 OS=Bovine papillomavirus OX=10571 GN=E1 PE=3 SV=1
MM1 pKa = 7.8 EE2 pKa = 5.38 ILSARR7 pKa = 11.84 LDD9 pKa = 3.71 ALQEE13 pKa = 4.38 DD14 pKa = 4.95 LLSLYY19 pKa = 10.06 EE20 pKa = 4.27 SNKK23 pKa = 10.14 NDD25 pKa = 2.98 IDD27 pKa = 4.01 SQIKK31 pKa = 9.43 HH32 pKa = 5.03 WMLLRR37 pKa = 11.84 KK38 pKa = 9.6 EE39 pKa = 4.22 YY40 pKa = 10.49 VIHH43 pKa = 6.43 YY44 pKa = 6.79 YY45 pKa = 10.95 ARR47 pKa = 11.84 ANSISRR53 pKa = 11.84 LGYY56 pKa = 10.19 QNILTQQVAQAKK68 pKa = 8.15 AHH70 pKa = 5.74 EE71 pKa = 4.93 AIEE74 pKa = 4.16 MVLNLEE80 pKa = 4.24 TLKK83 pKa = 10.91 ASPYY87 pKa = 10.42 GAEE90 pKa = 3.88 QWTLAQTSRR99 pKa = 11.84 EE100 pKa = 3.98 LWLTPPKK107 pKa = 10.58 HH108 pKa = 6.02 CFKK111 pKa = 11.04 KK112 pKa = 10.29 EE113 pKa = 3.66 GSTVEE118 pKa = 3.64 VWFDD122 pKa = 3.46 GRR124 pKa = 11.84 KK125 pKa = 9.93 DD126 pKa = 3.51 NAMHH130 pKa = 5.02 YY131 pKa = 6.87 TAWDD135 pKa = 3.65 KK136 pKa = 10.91 IYY138 pKa = 10.72 YY139 pKa = 7.67 QTDD142 pKa = 3.66 SAWIKK147 pKa = 9.81 TDD149 pKa = 2.81 GHH151 pKa = 7.13 VDD153 pKa = 3.38 YY154 pKa = 10.84 DD155 pKa = 3.65 GCFYY159 pKa = 11.55 YY160 pKa = 10.61 EE161 pKa = 4.03 GTYY164 pKa = 8.91 KK165 pKa = 10.3 HH166 pKa = 6.54 YY167 pKa = 10.64 YY168 pKa = 9.78 IKK170 pKa = 10.61 FQEE173 pKa = 4.65 DD174 pKa = 3.18 ADD176 pKa = 4.45 KK177 pKa = 11.36 YY178 pKa = 9.22 SQSGDD183 pKa = 3.08 WEE185 pKa = 4.5 VHH187 pKa = 5.56 FKK189 pKa = 10.66 HH190 pKa = 6.74 HH191 pKa = 6.08 VFSPVASVSSTTDD204 pKa = 2.99 GDD206 pKa = 4.11 PDD208 pKa = 4.08 PEE210 pKa = 5.86 CGLRR214 pKa = 11.84 ADD216 pKa = 3.72 VQRR219 pKa = 11.84 RR220 pKa = 11.84 VPEE223 pKa = 3.91 QHH225 pKa = 6.38 QVPLDD230 pKa = 3.66 NQATPRR236 pKa = 11.84 KK237 pKa = 9.23 RR238 pKa = 11.84 RR239 pKa = 11.84 RR240 pKa = 11.84 DD241 pKa = 3.52 EE242 pKa = 4.15 TGGDD246 pKa = 3.42 PAGPCTPRR254 pKa = 11.84 RR255 pKa = 11.84 KK256 pKa = 10.05 ARR258 pKa = 11.84 GDD260 pKa = 3.5 RR261 pKa = 11.84 CPHH264 pKa = 5.73 SRR266 pKa = 11.84 DD267 pKa = 3.06 TRR269 pKa = 11.84 GRR271 pKa = 11.84 RR272 pKa = 11.84 RR273 pKa = 11.84 GRR275 pKa = 11.84 DD276 pKa = 2.87 RR277 pKa = 11.84 GGGRR281 pKa = 11.84 GGRR284 pKa = 11.84 RR285 pKa = 11.84 QRR287 pKa = 11.84 RR288 pKa = 11.84 EE289 pKa = 3.38 GDD291 pKa = 3.43 SEE293 pKa = 3.93 EE294 pKa = 4.32 RR295 pKa = 11.84 QSQTEE300 pKa = 3.97 DD301 pKa = 3.35 SPDD304 pKa = 3.34 EE305 pKa = 4.21 RR306 pKa = 11.84 FHH308 pKa = 7.1 PGLTAAQQHH317 pKa = 5.63 GQRR320 pKa = 11.84 QRR322 pKa = 11.84 TTAEE326 pKa = 4.16 EE327 pKa = 4.68 DD328 pKa = 3.37 PHH330 pKa = 8.05 SAAPLYY336 pKa = 10.23 RR337 pKa = 11.84 QEE339 pKa = 4.11 EE340 pKa = 4.46 GDD342 pKa = 3.4 RR343 pKa = 11.84 GEE345 pKa = 4.38 ARR347 pKa = 11.84 QPYY350 pKa = 8.57 GSPRR354 pKa = 11.84 IPEE357 pKa = 4.08 EE358 pKa = 3.84 PAQQIDD364 pKa = 4.13 PNQLPRR370 pKa = 11.84 VRR372 pKa = 11.84 RR373 pKa = 11.84 QPARR377 pKa = 11.84 ILDD380 pKa = 3.91 HH381 pKa = 7.06 LADD384 pKa = 4.03 TPILLLRR391 pKa = 11.84 GGANQLKK398 pKa = 9.58 CYY400 pKa = 10.27 RR401 pKa = 11.84 YY402 pKa = 9.85 RR403 pKa = 11.84 LLKK406 pKa = 10.81 NYY408 pKa = 9.71 FNLFLDD414 pKa = 5.17 ISKK417 pKa = 7.8 TWQWIGAGGIGNKK430 pKa = 9.79 ARR432 pKa = 11.84 VTVAFEE438 pKa = 4.18 SLAQRR443 pKa = 11.84 NQFLNTVPIPTGVTVAWGRR462 pKa = 11.84 ISGLL466 pKa = 3.48
Molecular weight: 53.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.579
IPC2_protein 7.892
IPC_protein 7.819
Toseland 7.717
ProMoST 8.287
Dawson 8.492
Bjellqvist 8.697
Wikipedia 8.478
Rodwell 8.521
Grimsley 7.761
Solomon 8.609
Lehninger 8.595
Nozaki 8.77
DTASelect 8.507
Thurlkill 8.551
EMBOSS 8.682
Sillero 8.814
Patrickios 4.482
IPC_peptide 8.595
IPC2_peptide 7.644
IPC2.peptide.svr19 7.744
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2331
91
615
388.5
43.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.792 ± 0.73
2.402 ± 0.989
6.607 ± 0.309
6.349 ± 0.476
3.647 ± 0.436
6.65 ± 1.07
2.059 ± 0.582
4.59 ± 0.645
4.805 ± 0.57
8.237 ± 0.868
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.63 ± 0.545
4.505 ± 0.531
5.792 ± 1.078
5.234 ± 0.854
6.607 ± 0.82
6.735 ± 0.793
6.392 ± 1.064
6.392 ± 0.967
1.673 ± 0.461
3.904 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here