Hubei toti-like virus 16

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFH4|A0A1L3KFH4_9VIRU Uncharacterized protein OS=Hubei toti-like virus 16 OX=1923304 PE=4 SV=1
MM1 pKa = 7.63AFLAPLVIPALQALGFGGLTALGSAAVKK29 pKa = 10.29KK30 pKa = 10.74VIGDD34 pKa = 4.19AVPEE38 pKa = 4.19DD39 pKa = 3.67QQIALLTSTIGGQMDD54 pKa = 4.1PSSTQSLSPQLQSGLMEE71 pKa = 5.39LKK73 pKa = 9.54TAMGAAPTLFFSQEE87 pKa = 3.44ALAPRR92 pKa = 11.84SAQGFLNQPLVSSHH106 pKa = 6.18SDD108 pKa = 3.3QRR110 pKa = 11.84AHH112 pKa = 6.64SALSQTLSDD121 pKa = 3.99AAALSQLRR129 pKa = 11.84GMQLRR134 pKa = 11.84LQSIDD139 pKa = 3.25PMATTRR145 pKa = 11.84DD146 pKa = 3.57PGVAAFPGSTPSTSLRR162 pKa = 11.84EE163 pKa = 4.18TRR165 pKa = 11.84DD166 pKa = 3.37PMQGQQLPGTSPASWLGLQQVSPPTFQNQLPLGMSPDD203 pKa = 3.59HH204 pKa = 6.18QQGQTTSSTLSMIPGTNLLVAPQPINKK231 pKa = 9.12LQDD234 pKa = 3.34NQAVLTEE241 pKa = 4.43RR242 pKa = 11.84PPAAGTQNPVAQGISGVGGSPGLGNIVRR270 pKa = 11.84GAGTEE275 pKa = 3.62IFQGVKK281 pKa = 9.93EE282 pKa = 4.36AGQAAASQAVSQISGTATDD301 pKa = 4.17LAKK304 pKa = 10.74KK305 pKa = 10.31GLTKK309 pKa = 10.72AGGYY313 pKa = 9.56LGSLISSGLTSIAKK327 pKa = 9.9KK328 pKa = 10.29IIGDD332 pKa = 3.86APPGFEE338 pKa = 5.0GFDD341 pKa = 3.31ARR343 pKa = 11.84KK344 pKa = 10.03GGFIGPTLDD353 pKa = 4.6DD354 pKa = 3.79VFTGGFNKK362 pKa = 10.24YY363 pKa = 9.78GRR365 pKa = 11.84EE366 pKa = 3.87TPMALASNVDD376 pKa = 3.8LNKK379 pKa = 10.06IVGDD383 pKa = 4.23APPGEE388 pKa = 4.36QGEE391 pKa = 4.71VMTDD395 pKa = 3.12AQAAAATTNLTNSVKK410 pKa = 10.13FNMMSRR416 pKa = 11.84IILNEE421 pKa = 3.53QTLTQSSSLPRR432 pKa = 11.84GMSEE436 pKa = 3.79LQLNNFFARR445 pKa = 11.84DD446 pKa = 3.69LEE448 pKa = 4.63SLVGTYY454 pKa = 9.49KK455 pKa = 10.44QSEE458 pKa = 4.35VQALFGFVGMTCQYY472 pKa = 10.24NRR474 pKa = 11.84WPAAVYY480 pKa = 9.33TPEE483 pKa = 4.11TVEE486 pKa = 4.5SKK488 pKa = 9.36GTPKK492 pKa = 10.56EE493 pKa = 4.03KK494 pKa = 10.55VIPAHH499 pKa = 6.16NGVGTFSIDD508 pKa = 3.06ALYY511 pKa = 8.67CTGVALEE518 pKa = 4.33TARR521 pKa = 11.84VAPPCTPNVTLSQLGPLLAGAALGEE546 pKa = 4.36YY547 pKa = 10.21SSQLRR552 pKa = 11.84VKK554 pKa = 9.7TFSTDD559 pKa = 2.49MDD561 pKa = 3.97LMIRR565 pKa = 11.84ASAEE569 pKa = 3.67ALNNIQSQQGYY580 pKa = 8.11SFCLPLTKK588 pKa = 10.48AIGYY592 pKa = 7.76VLQCIQLFGSEE603 pKa = 4.28FNEE606 pKa = 4.57LVADD610 pKa = 4.04MYY612 pKa = 10.94FRR614 pKa = 11.84PTEE617 pKa = 4.2KK618 pKa = 10.44GQHH621 pKa = 5.94HH622 pKa = 7.13AFQRR626 pKa = 11.84AFPLEE631 pKa = 4.14TLAGTEE637 pKa = 3.83AWEE640 pKa = 4.38GDD642 pKa = 3.34DD643 pKa = 3.39WQGAKK648 pKa = 10.03FAVVCEE654 pKa = 4.0SDD656 pKa = 4.15FIKK659 pKa = 9.68VTCGIISGANWGEE672 pKa = 3.84FQPAEE677 pKa = 4.05WGHH680 pKa = 5.55NCAVVFIPNNDD691 pKa = 3.34KK692 pKa = 11.33SNQLMNAVRR701 pKa = 11.84MISQMAYY708 pKa = 8.96PAKK711 pKa = 10.3LLYY714 pKa = 10.02IHH716 pKa = 6.86GKK718 pKa = 6.42WAYY721 pKa = 9.53FSRR724 pKa = 11.84EE725 pKa = 4.05GEE727 pKa = 4.08LTTIGAEE734 pKa = 3.69VHH736 pKa = 5.49AQKK739 pKa = 10.83KK740 pKa = 10.28AGLTRR745 pKa = 11.84IPGPYY750 pKa = 9.31KK751 pKa = 9.51YY752 pKa = 10.89ALFVVMGARR761 pKa = 11.84EE762 pKa = 3.86QSSAGIIIGDD772 pKa = 3.59GSGQANHH779 pKa = 6.23QIEE782 pKa = 4.96VNAIDD787 pKa = 4.5HH788 pKa = 6.6NPTTPYY794 pKa = 10.72VCDD797 pKa = 3.7AGINGFSAARR807 pKa = 11.84NGAVTDD813 pKa = 3.77AAVPMWMAFIKK824 pKa = 9.76EE825 pKa = 4.01WEE827 pKa = 4.34VIYY830 pKa = 10.99GNASDD835 pKa = 4.63RR836 pKa = 11.84SSAMRR841 pKa = 11.84FWADD845 pKa = 3.1NSILFGNFFLGKK857 pKa = 9.84NPLDD861 pKa = 3.8IGDD864 pKa = 3.86LQGFSFLSWADD875 pKa = 3.75PVAHH879 pKa = 7.07PPLPSCLAEE888 pKa = 4.09YY889 pKa = 10.37TDD891 pKa = 6.23LPVSWSGSCKK901 pKa = 9.93QVTPQVARR909 pKa = 11.84YY910 pKa = 9.35LMSSMKK916 pKa = 8.92TASYY920 pKa = 9.82MPVASITPDD929 pKa = 3.41YY930 pKa = 10.96ASQRR934 pKa = 11.84PWTWGYY940 pKa = 11.31GNPPDD945 pKa = 4.33GCRR948 pKa = 11.84LPYY951 pKa = 9.67EE952 pKa = 4.28DD953 pKa = 4.92VMISFLVNKK962 pKa = 9.9KK963 pKa = 8.93WISPNEE969 pKa = 3.97EE970 pKa = 3.98YY971 pKa = 10.27PAVSLDD977 pKa = 3.21DD978 pKa = 3.9AARR981 pKa = 11.84LGHH984 pKa = 7.22TITAMANIMAAAADD998 pKa = 3.57ILVQEE1003 pKa = 4.85RR1004 pKa = 11.84DD1005 pKa = 3.4FTLPEE1010 pKa = 4.32VIFPPLVLQRR1020 pKa = 11.84VPMAMMRR1027 pKa = 11.84QKK1029 pKa = 11.0NVLWPALEE1037 pKa = 4.02EE1038 pKa = 4.11LLTNGISYY1046 pKa = 8.24PQYY1049 pKa = 9.59WNNRR1053 pKa = 11.84DD1054 pKa = 3.43VNMHH1058 pKa = 6.15CWEE1061 pKa = 3.92AMIYY1065 pKa = 9.68WEE1067 pKa = 4.67VAAQTIYY1074 pKa = 10.86PEE1076 pKa = 4.55TVSHH1080 pKa = 6.63GSAVTTSARR1089 pKa = 11.84IPVSVMGQWFAPLYY1103 pKa = 8.59PQYY1106 pKa = 10.78FQLRR1110 pKa = 11.84LNLQVMSPQRR1120 pKa = 11.84TYY1122 pKa = 11.1ISINNVARR1130 pKa = 11.84AMFKK1134 pKa = 10.57VEE1136 pKa = 4.09YY1137 pKa = 10.61AEE1139 pKa = 4.18EE1140 pKa = 4.34TTGLLGKK1147 pKa = 10.05SLSPVSWLMWRR1158 pKa = 11.84LSLGVIISDD1167 pKa = 3.37TVSANNGWIVQCVNNGGVFDD1187 pKa = 4.68YY1188 pKa = 9.88YY1189 pKa = 10.66IHH1191 pKa = 6.66MEE1193 pKa = 4.05QPDD1196 pKa = 3.4ASRR1199 pKa = 11.84NQVMCRR1205 pKa = 11.84EE1206 pKa = 4.09YY1207 pKa = 10.77GAGNVTVPPTTKK1219 pKa = 9.87ISLPTGEE1226 pKa = 5.35FILKK1230 pKa = 10.3RR1231 pKa = 11.84SIATGLQPFSLLMQGPRR1248 pKa = 11.84DD1249 pKa = 3.55AFIGVGTGSTAYY1261 pKa = 10.88QMFTQTNTPQAIFVSEE1277 pKa = 4.26TWATIPDD1284 pKa = 4.01WLFSPAGLQSVRR1296 pKa = 11.84DD1297 pKa = 4.1SII1299 pKa = 4.59

Molecular weight: 140.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KF79|A0A1L3KF79_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 16 OX=1923304 PE=4 SV=1
MM1 pKa = 7.74TDD3 pKa = 3.84GLPGAVGDD11 pKa = 4.48SGSSGSGFRR20 pKa = 11.84RR21 pKa = 11.84FDD23 pKa = 3.22SFGIGSGQEE32 pKa = 3.72GDD34 pKa = 3.45RR35 pKa = 11.84GRR37 pKa = 11.84STGGSTDD44 pKa = 3.15RR45 pKa = 11.84TTYY48 pKa = 10.42FYY50 pKa = 11.1DD51 pKa = 3.42RR52 pKa = 11.84RR53 pKa = 11.84PDD55 pKa = 3.35GSVKK59 pKa = 9.54YY60 pKa = 9.2TEE62 pKa = 5.26LEE64 pKa = 3.78PATTIRR70 pKa = 11.84VDD72 pKa = 4.31GIKK75 pKa = 10.38DD76 pKa = 3.61SYY78 pKa = 11.8GSGSNSLLFAGSSRR92 pKa = 11.84TSLSSGVFEE101 pKa = 4.58STFGQLAFGSARR113 pKa = 11.84PLRR116 pKa = 11.84PLTDD120 pKa = 3.31AQRR123 pKa = 11.84RR124 pKa = 11.84SGVVTIEE131 pKa = 4.06RR132 pKa = 11.84NATPSTVHH140 pKa = 6.68RR141 pKa = 11.84PDD143 pKa = 3.14GHH145 pKa = 6.11HH146 pKa = 5.44QGSRR150 pKa = 11.84RR151 pKa = 11.84SRR153 pKa = 11.84LSRR156 pKa = 11.84FDD158 pKa = 4.0PLYY161 pKa = 10.75KK162 pKa = 9.04PTRR165 pKa = 11.84NKK167 pKa = 10.57RR168 pKa = 11.84SDD170 pKa = 3.4AGSAAARR177 pKa = 11.84DD178 pKa = 3.71EE179 pKa = 4.31SGILARR185 pKa = 11.84IAAGVASNFSKK196 pKa = 10.7SASPRR201 pKa = 11.84YY202 pKa = 9.1EE203 pKa = 3.71PRR205 pKa = 11.84SPTGSDD211 pKa = 3.01NEE213 pKa = 4.17FDD215 pKa = 5.01IIDD218 pKa = 4.11DD219 pKa = 4.2PRR221 pKa = 11.84DD222 pKa = 3.24EE223 pKa = 4.46SARR226 pKa = 11.84SASADD231 pKa = 3.46QQTSGG236 pKa = 3.77

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2394

236

1299

798.0

87.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.354 ± 1.418

1.42 ± 0.352

5.388 ± 1.088

4.094 ± 0.104

4.094 ± 0.093

8.062 ± 0.883

1.337 ± 0.083

5.597 ± 0.563

3.968 ± 0.817

8.855 ± 0.771

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.614

4.135 ± 0.328

5.556 ± 0.923

4.637 ± 1.223

5.597 ± 1.646

9.148 ± 1.247

5.556 ± 0.924

7.185 ± 1.134

1.796 ± 0.29

2.632 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski