Red clover powdery mildew-associated totivirus 3
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S3Q2C1|A0A0S3Q2C1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 3 OX=1714364 GN=RdRp PE=3 SV=1
MM1 pKa = 7.29 YY2 pKa = 10.25 SQYY5 pKa = 11.13 LDD7 pKa = 3.99 TIFDD11 pKa = 3.59 TSRR14 pKa = 11.84 IANVVPYY21 pKa = 9.37 VAKK24 pKa = 10.67 GSYY27 pKa = 9.34 TLVNKK32 pKa = 10.22 ALAEE36 pKa = 4.16 LKK38 pKa = 11.12 VMDD41 pKa = 4.89 RR42 pKa = 11.84 SYY44 pKa = 11.51 LSQLEE49 pKa = 4.22 MSSDD53 pKa = 3.52 FQVAVFEE60 pKa = 4.94 PNVWTATASADD71 pKa = 3.36 YY72 pKa = 10.81 DD73 pKa = 4.0 GYY75 pKa = 10.84 NKK77 pKa = 10.15 KK78 pKa = 10.35 VLTSSGRR85 pKa = 11.84 FSSSQAYY92 pKa = 9.94 DD93 pKa = 3.22 EE94 pKa = 4.43 YY95 pKa = 11.38 ARR97 pKa = 11.84 SGSAIKK103 pKa = 9.91 IQHH106 pKa = 6.65 DD107 pKa = 3.26 KK108 pKa = 10.8 HH109 pKa = 7.86 YY110 pKa = 10.89 SIDD113 pKa = 3.53 LTAGDD118 pKa = 4.72 RR119 pKa = 11.84 DD120 pKa = 3.85 SQEE123 pKa = 4.22 AFIFNMLVSWFKK135 pKa = 11.56 AEE137 pKa = 3.85 ITRR140 pKa = 11.84 DD141 pKa = 3.93 CEE143 pKa = 4.18 PEE145 pKa = 3.89 DD146 pKa = 3.69 LFVRR150 pKa = 11.84 QHH152 pKa = 5.41 SFKK155 pKa = 10.75 DD156 pKa = 3.51 SHH158 pKa = 8.07 VEE160 pKa = 3.44 NAMADD165 pKa = 3.14 GGMEE169 pKa = 3.98 GKK171 pKa = 10.23 VEE173 pKa = 3.88 IRR175 pKa = 11.84 LGAPAPEE182 pKa = 4.02 RR183 pKa = 11.84 LKK185 pKa = 10.91 NALFVEE191 pKa = 4.49 RR192 pKa = 11.84 NDD194 pKa = 3.56 LNFWTKK200 pKa = 10.46 PYY202 pKa = 9.38 VVKK205 pKa = 11.1 YY206 pKa = 10.35 NALTHH211 pKa = 5.63 EE212 pKa = 4.07 QQAFYY217 pKa = 9.91 LAHH220 pKa = 5.74 VMGRR224 pKa = 11.84 TEE226 pKa = 4.17 TDD228 pKa = 3.94 GITADD233 pKa = 3.32 ISIPGVMVQDD243 pKa = 4.45 FLFEE247 pKa = 4.88 PIGVEE252 pKa = 3.65 LTRR255 pKa = 11.84 DD256 pKa = 3.02 IDD258 pKa = 3.88 YY259 pKa = 11.49 SSVDD263 pKa = 2.93 WRR265 pKa = 11.84 ASDD268 pKa = 4.89 TMMAWIRR275 pKa = 11.84 DD276 pKa = 3.75 YY277 pKa = 11.86 VEE279 pKa = 5.26 LNRR282 pKa = 11.84 CYY284 pKa = 10.52 KK285 pKa = 10.64 AFAAACDD292 pKa = 4.0 LLGTLAFNPAPSFHH306 pKa = 6.94 EE307 pKa = 4.65 SIWWNPLVRR316 pKa = 11.84 IVYY319 pKa = 7.81 LAPFTPTRR327 pKa = 11.84 ARR329 pKa = 11.84 VPTTLNGEE337 pKa = 4.11 GMYY340 pKa = 10.45 EE341 pKa = 4.17 SEE343 pKa = 4.42 TILNFFRR350 pKa = 11.84 NDD352 pKa = 2.99 ACDD355 pKa = 3.29 MKK357 pKa = 11.14 NFIVMSSLVNYY368 pKa = 8.6 ANHH371 pKa = 5.91 MALYY375 pKa = 9.44 GLLMNEE381 pKa = 4.37 RR382 pKa = 11.84 QDD384 pKa = 3.52 LTDD387 pKa = 3.39 WKK389 pKa = 9.86 ICLSAIIGSMSNMRR403 pKa = 11.84 GPLARR408 pKa = 11.84 AQLFSLITNRR418 pKa = 11.84 EE419 pKa = 4.11 VEE421 pKa = 4.63 SMTTNNCYY429 pKa = 10.5 LEE431 pKa = 4.51 YY432 pKa = 11.14 DD433 pKa = 3.7 MSDD436 pKa = 3.24 MTGGALTVEE445 pKa = 4.45 FDD447 pKa = 3.66 VLKK450 pKa = 10.76 GDD452 pKa = 3.92 NQAPTAKK459 pKa = 9.79 IINPVPYY466 pKa = 10.51 VSGALFAGACGLDD479 pKa = 3.16 VDD481 pKa = 4.48 GFEE484 pKa = 6.11 HH485 pKa = 7.06 IMPRR489 pKa = 11.84 QTIEE493 pKa = 3.69 IPKK496 pKa = 10.16 SGNLYY501 pKa = 9.88 RR502 pKa = 11.84 DD503 pKa = 3.34 DD504 pKa = 4.1 VFRR507 pKa = 11.84 LAAAYY512 pKa = 9.83 RR513 pKa = 11.84 YY514 pKa = 9.16 FGHH517 pKa = 6.62 EE518 pKa = 4.07 LDD520 pKa = 3.65 ISAVRR525 pKa = 11.84 DD526 pKa = 3.59 RR527 pKa = 11.84 TRR529 pKa = 11.84 FSHH532 pKa = 5.61 WCNGRR537 pKa = 11.84 EE538 pKa = 3.95 LVLAKK543 pKa = 10.66 YY544 pKa = 9.45 PILNAYY550 pKa = 10.19 DD551 pKa = 4.16 EE552 pKa = 4.23 DD553 pKa = 4.29 TKK555 pKa = 11.38 FKK557 pKa = 10.71 VHH559 pKa = 5.96 GSKK562 pKa = 10.2 RR563 pKa = 11.84 RR564 pKa = 11.84 PGRR567 pKa = 11.84 HH568 pKa = 6.67 DD569 pKa = 3.94 DD570 pKa = 3.74 VLEE573 pKa = 4.01 ASYY576 pKa = 11.19 LRR578 pKa = 11.84 GGDD581 pKa = 3.49 VVEE584 pKa = 4.65 LVISRR589 pKa = 11.84 PQIIMAGYY597 pKa = 6.68 RR598 pKa = 11.84 TRR600 pKa = 11.84 RR601 pKa = 11.84 EE602 pKa = 3.86 IPTVASRR609 pKa = 11.84 GVRR612 pKa = 11.84 RR613 pKa = 11.84 AKK615 pKa = 10.65 VLLPKK620 pKa = 10.66 VKK622 pKa = 10.27 GAASSKK628 pKa = 9.23 RR629 pKa = 11.84 VSVRR633 pKa = 11.84 ASALRR638 pKa = 11.84 EE639 pKa = 3.73 KK640 pKa = 10.75 GDD642 pKa = 3.3 RR643 pKa = 11.84 HH644 pKa = 6.18 EE645 pKa = 5.04 SDD647 pKa = 3.62 FPEE650 pKa = 4.51 AQVGQAPSNPILNTSDD666 pKa = 4.03 ASILEE671 pKa = 3.94 QLNVDD676 pKa = 4.46 LGQAAEE682 pKa = 4.15 NAGG685 pKa = 3.51
Molecular weight: 76.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.37
IPC2_protein 5.385
IPC_protein 5.397
Toseland 5.575
ProMoST 5.639
Dawson 5.512
Bjellqvist 5.588
Wikipedia 5.448
Rodwell 5.461
Grimsley 5.614
Solomon 5.512
Lehninger 5.486
Nozaki 5.703
DTASelect 5.893
Thurlkill 5.703
EMBOSS 5.677
Sillero 5.792
Patrickios 4.05
IPC_peptide 5.525
IPC2_peptide 5.792
IPC2.peptide.svr19 5.758
Protein with the highest isoelectric point:
>tr|A0A0S3Q2C1|A0A0S3Q2C1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 3 OX=1714364 GN=RdRp PE=3 SV=1
MM1 pKa = 6.69 TWKK4 pKa = 10.89 AEE6 pKa = 4.36 DD7 pKa = 4.15 GVPMCIALAKK17 pKa = 10.71 DD18 pKa = 3.4 GANWNPSVFNLATHH32 pKa = 5.55 VFARR36 pKa = 11.84 VTEE39 pKa = 4.13 SLAMEE44 pKa = 4.23 GTEE47 pKa = 4.52 VNTLLFGTITPAVHH61 pKa = 6.59 IKK63 pKa = 10.34 VGQLNCLYY71 pKa = 10.83 LKK73 pKa = 10.06 IDD75 pKa = 3.56 QCCVTTHH82 pKa = 6.92 PALNIILSRR91 pKa = 11.84 HH92 pKa = 4.7 YY93 pKa = 9.72 SQLYY97 pKa = 8.96 EE98 pKa = 4.18 SVDD101 pKa = 4.04 FVNPMSDD108 pKa = 2.5 SMLFRR113 pKa = 11.84 KK114 pKa = 10.04 RR115 pKa = 11.84 GINAEE120 pKa = 4.4 DD121 pKa = 4.08 GPEE124 pKa = 3.96 PVRR127 pKa = 11.84 GLLAMSKK134 pKa = 9.72 LPHH137 pKa = 6.3 SAITQQHH144 pKa = 6.19 HH145 pKa = 4.98 LHH147 pKa = 6.26 FNITEE152 pKa = 4.26 ACQTIDD158 pKa = 3.94 RR159 pKa = 11.84 NRR161 pKa = 11.84 LSYY164 pKa = 10.76 LLEE167 pKa = 4.25 VLSVPSDD174 pKa = 3.26 ATQAFASGLFMWGALVGDD192 pKa = 3.94 AMFEE196 pKa = 4.13 RR197 pKa = 11.84 VRR199 pKa = 11.84 EE200 pKa = 4.09 SGLFGSRR207 pKa = 11.84 SSAQFAAIAKK217 pKa = 8.72 QISVEE222 pKa = 4.13 AKK224 pKa = 9.98 SLQNLHH230 pKa = 5.95 TEE232 pKa = 4.47 DD233 pKa = 3.48 YY234 pKa = 10.95 RR235 pKa = 11.84 SIFEE239 pKa = 5.03 LDD241 pKa = 3.35 VLVNRR246 pKa = 11.84 GVGKK250 pKa = 10.57 VDD252 pKa = 3.23 WEE254 pKa = 4.19 KK255 pKa = 10.47 EE256 pKa = 3.93 QEE258 pKa = 4.07 NRR260 pKa = 11.84 QHH262 pKa = 6.55 PKK264 pKa = 9.78 VATVDD269 pKa = 3.14 ACEE272 pKa = 4.16 VRR274 pKa = 11.84 RR275 pKa = 11.84 TAYY278 pKa = 10.54 LVFRR282 pKa = 11.84 KK283 pKa = 9.96 ALEE286 pKa = 3.79 QGRR289 pKa = 11.84 RR290 pKa = 11.84 PVSMSWDD297 pKa = 3.96 EE298 pKa = 3.86 FWSARR303 pKa = 11.84 WEE305 pKa = 3.91 WTAAGAVHH313 pKa = 6.67 SNAPGDD319 pKa = 3.52 SKK321 pKa = 11.73 YY322 pKa = 10.64 IFKK325 pKa = 10.4 EE326 pKa = 3.9 VNEE329 pKa = 4.39 MKK331 pKa = 10.82 NKK333 pKa = 9.86 FITMSAMPKK342 pKa = 10.22 LPLSHH347 pKa = 7.5 FLQRR351 pKa = 11.84 EE352 pKa = 3.82 PGIDD356 pKa = 2.73 GWASVKK362 pKa = 10.48 YY363 pKa = 9.61 EE364 pKa = 3.78 WGKK367 pKa = 9.96 QRR369 pKa = 11.84 AIYY372 pKa = 8.53 GTDD375 pKa = 2.92 LTSYY379 pKa = 10.02 VLSNFAMYY387 pKa = 10.32 ACEE390 pKa = 4.25 SVLPSAFPVGPDD402 pKa = 3.01 ADD404 pKa = 4.17 EE405 pKa = 5.04 ANVAARR411 pKa = 11.84 VGGVLNNRR419 pKa = 11.84 MPLCIDD425 pKa = 3.84 FEE427 pKa = 4.92 DD428 pKa = 5.15 FNSQHH433 pKa = 6.14 SVEE436 pKa = 4.58 AMQAVLDD443 pKa = 4.57 AYY445 pKa = 11.41 SDD447 pKa = 3.98 VFDD450 pKa = 4.61 HH451 pKa = 7.13 LLTVEE456 pKa = 3.95 QKK458 pKa = 10.72 YY459 pKa = 10.5 ALRR462 pKa = 11.84 WTAQSIGKK470 pKa = 8.21 TSITNNIANCGTYY483 pKa = 7.94 STKK486 pKa = 10.03 GTMLSGWRR494 pKa = 11.84 LTTMMNSVLNYY505 pKa = 10.19 VYY507 pKa = 7.65 TTHH510 pKa = 7.61 LLGRR514 pKa = 11.84 DD515 pKa = 3.39 RR516 pKa = 11.84 DD517 pKa = 4.2 DD518 pKa = 3.63 TPSVHH523 pKa = 6.78 NGDD526 pKa = 4.41 DD527 pKa = 3.38 VLLGVKK533 pKa = 9.62 NMRR536 pKa = 11.84 VAQEE540 pKa = 3.88 MNRR543 pKa = 11.84 RR544 pKa = 11.84 AQRR547 pKa = 11.84 VGIRR551 pKa = 11.84 LQPSKK556 pKa = 10.92 CAFAGIAEE564 pKa = 4.62 FLRR567 pKa = 11.84 VDD569 pKa = 4.78 RR570 pKa = 11.84 IRR572 pKa = 11.84 GSGGQYY578 pKa = 8.46 LTRR581 pKa = 11.84 SVATIVHH588 pKa = 5.87 SRR590 pKa = 11.84 VEE592 pKa = 4.58 SKK594 pKa = 10.64 PKK596 pKa = 10.47 PNVKK600 pKa = 10.27 DD601 pKa = 4.18 LIEE604 pKa = 4.56 AMEE607 pKa = 4.05 TRR609 pKa = 11.84 LADD612 pKa = 3.45 LHH614 pKa = 6.37 QRR616 pKa = 11.84 GASIRR621 pKa = 11.84 KK622 pKa = 7.2 IARR625 pKa = 11.84 IRR627 pKa = 11.84 EE628 pKa = 4.14 VYY630 pKa = 8.18 YY631 pKa = 10.84 HH632 pKa = 7.0 KK633 pKa = 10.72 LAGIFKK639 pKa = 10.25 SEE641 pKa = 3.89 VDD643 pKa = 2.95 TMYY646 pKa = 10.73 AIKK649 pKa = 10.36 SIHH652 pKa = 6.22 RR653 pKa = 11.84 VNGGISQDD661 pKa = 2.9 MYY663 pKa = 10.58 AAVDD667 pKa = 3.75 RR668 pKa = 11.84 EE669 pKa = 4.18 VLNVVNLEE677 pKa = 4.07 KK678 pKa = 10.9 SSVPEE683 pKa = 3.98 LPKK686 pKa = 10.84 LPGIHH691 pKa = 7.69 DD692 pKa = 3.81 YY693 pKa = 11.63 ADD695 pKa = 3.55 YY696 pKa = 10.18 VAKK699 pKa = 10.48 VLPIEE704 pKa = 4.4 SKK706 pKa = 9.91 VKK708 pKa = 10.42 KK709 pKa = 9.41 MCEE712 pKa = 3.87 KK713 pKa = 9.7 LTKK716 pKa = 9.38 STQDD720 pKa = 3.18 SVSMYY725 pKa = 8.52 FTTAVLADD733 pKa = 3.86 CTSVQDD739 pKa = 3.8 RR740 pKa = 11.84 LNMKK744 pKa = 10.08 KK745 pKa = 9.83 IYY747 pKa = 9.32 KK748 pKa = 8.63 AHH750 pKa = 6.43 KK751 pKa = 10.28 GEE753 pKa = 4.38 IKK755 pKa = 10.22 VSQYY759 pKa = 10.77 GKK761 pKa = 10.7 AVLTGYY767 pKa = 11.05 GMEE770 pKa = 4.53 LLRR773 pKa = 11.84 SRR775 pKa = 11.84 LRR777 pKa = 11.84 PGALRR782 pKa = 11.84 TLLTRR787 pKa = 11.84 SEE789 pKa = 4.17 NPIEE793 pKa = 4.15 LLSLVVV799 pKa = 3.63
Molecular weight: 89.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.548
IPC2_protein 7.892
IPC_protein 7.717
Toseland 7.556
ProMoST 8.302
Dawson 8.492
Bjellqvist 8.741
Wikipedia 8.448
Rodwell 8.536
Grimsley 7.614
Solomon 8.551
Lehninger 8.551
Nozaki 8.887
DTASelect 8.521
Thurlkill 8.595
EMBOSS 8.682
Sillero 8.873
Patrickios 4.482
IPC_peptide 8.551
IPC2_peptide 7.585
IPC2.peptide.svr19 7.791
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1484
685
799
742.0
83.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.03 ± 0.323
1.348 ± 0.126
5.93 ± 0.757
5.795 ± 0.031
3.774 ± 0.426
5.863 ± 0.016
2.426 ± 0.268
4.919 ± 0.134
4.919 ± 0.584
8.693 ± 0.363
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.302 ± 0.063
4.582 ± 0.165
3.841 ± 0.173
3.167 ± 0.276
6.469 ± 0.173
7.547 ± 0.072
5.256 ± 0.205
7.884 ± 0.513
1.415 ± 0.071
3.841 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here