Red clover powdery mildew-associated totivirus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; unclassified Totiviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0S3Q2C1|A0A0S3Q2C1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 3 OX=1714364 GN=RdRp PE=3 SV=1
MM1 pKa = 7.29YY2 pKa = 10.25SQYY5 pKa = 11.13LDD7 pKa = 3.99TIFDD11 pKa = 3.59TSRR14 pKa = 11.84IANVVPYY21 pKa = 9.37VAKK24 pKa = 10.67GSYY27 pKa = 9.34TLVNKK32 pKa = 10.22ALAEE36 pKa = 4.16LKK38 pKa = 11.12VMDD41 pKa = 4.89RR42 pKa = 11.84SYY44 pKa = 11.51LSQLEE49 pKa = 4.22MSSDD53 pKa = 3.52FQVAVFEE60 pKa = 4.94PNVWTATASADD71 pKa = 3.36YY72 pKa = 10.81DD73 pKa = 4.0GYY75 pKa = 10.84NKK77 pKa = 10.15KK78 pKa = 10.35VLTSSGRR85 pKa = 11.84FSSSQAYY92 pKa = 9.94DD93 pKa = 3.22EE94 pKa = 4.43YY95 pKa = 11.38ARR97 pKa = 11.84SGSAIKK103 pKa = 9.91IQHH106 pKa = 6.65DD107 pKa = 3.26KK108 pKa = 10.8HH109 pKa = 7.86YY110 pKa = 10.89SIDD113 pKa = 3.53LTAGDD118 pKa = 4.72RR119 pKa = 11.84DD120 pKa = 3.85SQEE123 pKa = 4.22AFIFNMLVSWFKK135 pKa = 11.56AEE137 pKa = 3.85ITRR140 pKa = 11.84DD141 pKa = 3.93CEE143 pKa = 4.18PEE145 pKa = 3.89DD146 pKa = 3.69LFVRR150 pKa = 11.84QHH152 pKa = 5.41SFKK155 pKa = 10.75DD156 pKa = 3.51SHH158 pKa = 8.07VEE160 pKa = 3.44NAMADD165 pKa = 3.14GGMEE169 pKa = 3.98GKK171 pKa = 10.23VEE173 pKa = 3.88IRR175 pKa = 11.84LGAPAPEE182 pKa = 4.02RR183 pKa = 11.84LKK185 pKa = 10.91NALFVEE191 pKa = 4.49RR192 pKa = 11.84NDD194 pKa = 3.56LNFWTKK200 pKa = 10.46PYY202 pKa = 9.38VVKK205 pKa = 11.1YY206 pKa = 10.35NALTHH211 pKa = 5.63EE212 pKa = 4.07QQAFYY217 pKa = 9.91LAHH220 pKa = 5.74VMGRR224 pKa = 11.84TEE226 pKa = 4.17TDD228 pKa = 3.94GITADD233 pKa = 3.32ISIPGVMVQDD243 pKa = 4.45FLFEE247 pKa = 4.88PIGVEE252 pKa = 3.65LTRR255 pKa = 11.84DD256 pKa = 3.02IDD258 pKa = 3.88YY259 pKa = 11.49SSVDD263 pKa = 2.93WRR265 pKa = 11.84ASDD268 pKa = 4.89TMMAWIRR275 pKa = 11.84DD276 pKa = 3.75YY277 pKa = 11.86VEE279 pKa = 5.26LNRR282 pKa = 11.84CYY284 pKa = 10.52KK285 pKa = 10.64AFAAACDD292 pKa = 4.0LLGTLAFNPAPSFHH306 pKa = 6.94EE307 pKa = 4.65SIWWNPLVRR316 pKa = 11.84IVYY319 pKa = 7.81LAPFTPTRR327 pKa = 11.84ARR329 pKa = 11.84VPTTLNGEE337 pKa = 4.11GMYY340 pKa = 10.45EE341 pKa = 4.17SEE343 pKa = 4.42TILNFFRR350 pKa = 11.84NDD352 pKa = 2.99ACDD355 pKa = 3.29MKK357 pKa = 11.14NFIVMSSLVNYY368 pKa = 8.6ANHH371 pKa = 5.91MALYY375 pKa = 9.44GLLMNEE381 pKa = 4.37RR382 pKa = 11.84QDD384 pKa = 3.52LTDD387 pKa = 3.39WKK389 pKa = 9.86ICLSAIIGSMSNMRR403 pKa = 11.84GPLARR408 pKa = 11.84AQLFSLITNRR418 pKa = 11.84EE419 pKa = 4.11VEE421 pKa = 4.63SMTTNNCYY429 pKa = 10.5LEE431 pKa = 4.51YY432 pKa = 11.14DD433 pKa = 3.7MSDD436 pKa = 3.24MTGGALTVEE445 pKa = 4.45FDD447 pKa = 3.66VLKK450 pKa = 10.76GDD452 pKa = 3.92NQAPTAKK459 pKa = 9.79IINPVPYY466 pKa = 10.51VSGALFAGACGLDD479 pKa = 3.16VDD481 pKa = 4.48GFEE484 pKa = 6.11HH485 pKa = 7.06IMPRR489 pKa = 11.84QTIEE493 pKa = 3.69IPKK496 pKa = 10.16SGNLYY501 pKa = 9.88RR502 pKa = 11.84DD503 pKa = 3.34DD504 pKa = 4.1VFRR507 pKa = 11.84LAAAYY512 pKa = 9.83RR513 pKa = 11.84YY514 pKa = 9.16FGHH517 pKa = 6.62EE518 pKa = 4.07LDD520 pKa = 3.65ISAVRR525 pKa = 11.84DD526 pKa = 3.59RR527 pKa = 11.84TRR529 pKa = 11.84FSHH532 pKa = 5.61WCNGRR537 pKa = 11.84EE538 pKa = 3.95LVLAKK543 pKa = 10.66YY544 pKa = 9.45PILNAYY550 pKa = 10.19DD551 pKa = 4.16EE552 pKa = 4.23DD553 pKa = 4.29TKK555 pKa = 11.38FKK557 pKa = 10.71VHH559 pKa = 5.96GSKK562 pKa = 10.2RR563 pKa = 11.84RR564 pKa = 11.84PGRR567 pKa = 11.84HH568 pKa = 6.67DD569 pKa = 3.94DD570 pKa = 3.74VLEE573 pKa = 4.01ASYY576 pKa = 11.19LRR578 pKa = 11.84GGDD581 pKa = 3.49VVEE584 pKa = 4.65LVISRR589 pKa = 11.84PQIIMAGYY597 pKa = 6.68RR598 pKa = 11.84TRR600 pKa = 11.84RR601 pKa = 11.84EE602 pKa = 3.86IPTVASRR609 pKa = 11.84GVRR612 pKa = 11.84RR613 pKa = 11.84AKK615 pKa = 10.65VLLPKK620 pKa = 10.66VKK622 pKa = 10.27GAASSKK628 pKa = 9.23RR629 pKa = 11.84VSVRR633 pKa = 11.84ASALRR638 pKa = 11.84EE639 pKa = 3.73KK640 pKa = 10.75GDD642 pKa = 3.3RR643 pKa = 11.84HH644 pKa = 6.18EE645 pKa = 5.04SDD647 pKa = 3.62FPEE650 pKa = 4.51AQVGQAPSNPILNTSDD666 pKa = 4.03ASILEE671 pKa = 3.94QLNVDD676 pKa = 4.46LGQAAEE682 pKa = 4.15NAGG685 pKa = 3.51

Molecular weight:
76.95 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0S3Q2C1|A0A0S3Q2C1_9VIRU RNA-directed RNA polymerase OS=Red clover powdery mildew-associated totivirus 3 OX=1714364 GN=RdRp PE=3 SV=1
MM1 pKa = 6.69TWKK4 pKa = 10.89AEE6 pKa = 4.36DD7 pKa = 4.15GVPMCIALAKK17 pKa = 10.71DD18 pKa = 3.4GANWNPSVFNLATHH32 pKa = 5.55VFARR36 pKa = 11.84VTEE39 pKa = 4.13SLAMEE44 pKa = 4.23GTEE47 pKa = 4.52VNTLLFGTITPAVHH61 pKa = 6.59IKK63 pKa = 10.34VGQLNCLYY71 pKa = 10.83LKK73 pKa = 10.06IDD75 pKa = 3.56QCCVTTHH82 pKa = 6.92PALNIILSRR91 pKa = 11.84HH92 pKa = 4.7YY93 pKa = 9.72SQLYY97 pKa = 8.96EE98 pKa = 4.18SVDD101 pKa = 4.04FVNPMSDD108 pKa = 2.5SMLFRR113 pKa = 11.84KK114 pKa = 10.04RR115 pKa = 11.84GINAEE120 pKa = 4.4DD121 pKa = 4.08GPEE124 pKa = 3.96PVRR127 pKa = 11.84GLLAMSKK134 pKa = 9.72LPHH137 pKa = 6.3SAITQQHH144 pKa = 6.19HH145 pKa = 4.98LHH147 pKa = 6.26FNITEE152 pKa = 4.26ACQTIDD158 pKa = 3.94RR159 pKa = 11.84NRR161 pKa = 11.84LSYY164 pKa = 10.76LLEE167 pKa = 4.25VLSVPSDD174 pKa = 3.26ATQAFASGLFMWGALVGDD192 pKa = 3.94AMFEE196 pKa = 4.13RR197 pKa = 11.84VRR199 pKa = 11.84EE200 pKa = 4.09SGLFGSRR207 pKa = 11.84SSAQFAAIAKK217 pKa = 8.72QISVEE222 pKa = 4.13AKK224 pKa = 9.98SLQNLHH230 pKa = 5.95TEE232 pKa = 4.47DD233 pKa = 3.48YY234 pKa = 10.95RR235 pKa = 11.84SIFEE239 pKa = 5.03LDD241 pKa = 3.35VLVNRR246 pKa = 11.84GVGKK250 pKa = 10.57VDD252 pKa = 3.23WEE254 pKa = 4.19KK255 pKa = 10.47EE256 pKa = 3.93QEE258 pKa = 4.07NRR260 pKa = 11.84QHH262 pKa = 6.55PKK264 pKa = 9.78VATVDD269 pKa = 3.14ACEE272 pKa = 4.16VRR274 pKa = 11.84RR275 pKa = 11.84TAYY278 pKa = 10.54LVFRR282 pKa = 11.84KK283 pKa = 9.96ALEE286 pKa = 3.79QGRR289 pKa = 11.84RR290 pKa = 11.84PVSMSWDD297 pKa = 3.96EE298 pKa = 3.86FWSARR303 pKa = 11.84WEE305 pKa = 3.91WTAAGAVHH313 pKa = 6.67SNAPGDD319 pKa = 3.52SKK321 pKa = 11.73YY322 pKa = 10.64IFKK325 pKa = 10.4EE326 pKa = 3.9VNEE329 pKa = 4.39MKK331 pKa = 10.82NKK333 pKa = 9.86FITMSAMPKK342 pKa = 10.22LPLSHH347 pKa = 7.5FLQRR351 pKa = 11.84EE352 pKa = 3.82PGIDD356 pKa = 2.73GWASVKK362 pKa = 10.48YY363 pKa = 9.61EE364 pKa = 3.78WGKK367 pKa = 9.96QRR369 pKa = 11.84AIYY372 pKa = 8.53GTDD375 pKa = 2.92LTSYY379 pKa = 10.02VLSNFAMYY387 pKa = 10.32ACEE390 pKa = 4.25SVLPSAFPVGPDD402 pKa = 3.01ADD404 pKa = 4.17EE405 pKa = 5.04ANVAARR411 pKa = 11.84VGGVLNNRR419 pKa = 11.84MPLCIDD425 pKa = 3.84FEE427 pKa = 4.92DD428 pKa = 5.15FNSQHH433 pKa = 6.14SVEE436 pKa = 4.58AMQAVLDD443 pKa = 4.57AYY445 pKa = 11.41SDD447 pKa = 3.98VFDD450 pKa = 4.61HH451 pKa = 7.13LLTVEE456 pKa = 3.95QKK458 pKa = 10.72YY459 pKa = 10.5ALRR462 pKa = 11.84WTAQSIGKK470 pKa = 8.21TSITNNIANCGTYY483 pKa = 7.94STKK486 pKa = 10.03GTMLSGWRR494 pKa = 11.84LTTMMNSVLNYY505 pKa = 10.19VYY507 pKa = 7.65TTHH510 pKa = 7.61LLGRR514 pKa = 11.84DD515 pKa = 3.39RR516 pKa = 11.84DD517 pKa = 4.2DD518 pKa = 3.63TPSVHH523 pKa = 6.78NGDD526 pKa = 4.41DD527 pKa = 3.38VLLGVKK533 pKa = 9.62NMRR536 pKa = 11.84VAQEE540 pKa = 3.88MNRR543 pKa = 11.84RR544 pKa = 11.84AQRR547 pKa = 11.84VGIRR551 pKa = 11.84LQPSKK556 pKa = 10.92CAFAGIAEE564 pKa = 4.62FLRR567 pKa = 11.84VDD569 pKa = 4.78RR570 pKa = 11.84IRR572 pKa = 11.84GSGGQYY578 pKa = 8.46LTRR581 pKa = 11.84SVATIVHH588 pKa = 5.87SRR590 pKa = 11.84VEE592 pKa = 4.58SKK594 pKa = 10.64PKK596 pKa = 10.47PNVKK600 pKa = 10.27DD601 pKa = 4.18LIEE604 pKa = 4.56AMEE607 pKa = 4.05TRR609 pKa = 11.84LADD612 pKa = 3.45LHH614 pKa = 6.37QRR616 pKa = 11.84GASIRR621 pKa = 11.84KK622 pKa = 7.2IARR625 pKa = 11.84IRR627 pKa = 11.84EE628 pKa = 4.14VYY630 pKa = 8.18YY631 pKa = 10.84HH632 pKa = 7.0KK633 pKa = 10.72LAGIFKK639 pKa = 10.25SEE641 pKa = 3.89VDD643 pKa = 2.95TMYY646 pKa = 10.73AIKK649 pKa = 10.36SIHH652 pKa = 6.22RR653 pKa = 11.84VNGGISQDD661 pKa = 2.9MYY663 pKa = 10.58AAVDD667 pKa = 3.75RR668 pKa = 11.84EE669 pKa = 4.18VLNVVNLEE677 pKa = 4.07KK678 pKa = 10.9SSVPEE683 pKa = 3.98LPKK686 pKa = 10.84LPGIHH691 pKa = 7.69DD692 pKa = 3.81YY693 pKa = 11.63ADD695 pKa = 3.55YY696 pKa = 10.18VAKK699 pKa = 10.48VLPIEE704 pKa = 4.4SKK706 pKa = 9.91VKK708 pKa = 10.42KK709 pKa = 9.41MCEE712 pKa = 3.87KK713 pKa = 9.7LTKK716 pKa = 9.38STQDD720 pKa = 3.18SVSMYY725 pKa = 8.52FTTAVLADD733 pKa = 3.86CTSVQDD739 pKa = 3.8RR740 pKa = 11.84LNMKK744 pKa = 10.08KK745 pKa = 9.83IYY747 pKa = 9.32KK748 pKa = 8.63AHH750 pKa = 6.43KK751 pKa = 10.28GEE753 pKa = 4.38IKK755 pKa = 10.22VSQYY759 pKa = 10.77GKK761 pKa = 10.7AVLTGYY767 pKa = 11.05GMEE770 pKa = 4.53LLRR773 pKa = 11.84SRR775 pKa = 11.84LRR777 pKa = 11.84PGALRR782 pKa = 11.84TLLTRR787 pKa = 11.84SEE789 pKa = 4.17NPIEE793 pKa = 4.15LLSLVVV799 pKa = 3.63

Molecular weight:
89.45 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1484

685

799

742.0

83.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.03 ± 0.323

1.348 ± 0.126

5.93 ± 0.757

5.795 ± 0.031

3.774 ± 0.426

5.863 ± 0.016

2.426 ± 0.268

4.919 ± 0.134

4.919 ± 0.584

8.693 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.302 ± 0.063

4.582 ± 0.165

3.841 ± 0.173

3.167 ± 0.276

6.469 ± 0.173

7.547 ± 0.072

5.256 ± 0.205

7.884 ± 0.513

1.415 ± 0.071

3.841 ± 0.378

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski