Mycobacterium phage Joselito

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H1X9|A0A2Z5H1X9_9CAUD Uncharacterized protein OS=Mycobacterium phage Joselito OX=2234075 GN=74 PE=4 SV=1
MM1 pKa = 7.18ITFEE5 pKa = 3.97QARR8 pKa = 11.84QIVFDD13 pKa = 4.28NLAGQYY19 pKa = 9.84PPEE22 pKa = 5.96ADD24 pKa = 3.45FQVATWGWEE33 pKa = 3.85NDD35 pKa = 3.46DD36 pKa = 5.35LYY38 pKa = 11.58QLVAGPYY45 pKa = 9.04AMVYY49 pKa = 9.75VSRR52 pKa = 11.84GPQDD56 pKa = 5.02DD57 pKa = 3.8EE58 pKa = 4.05WLEE61 pKa = 4.75DD62 pKa = 3.62GDD64 pKa = 5.06GPFIMVNKK72 pKa = 8.13EE73 pKa = 3.55TGEE76 pKa = 4.06YY77 pKa = 9.09IEE79 pKa = 5.37EE80 pKa = 4.2YY81 pKa = 10.67GEE83 pKa = 4.28PFLIPDD89 pKa = 3.73ARR91 pKa = 11.84TIGEE95 pKa = 4.19PQPAPP100 pKa = 3.39

Molecular weight: 11.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H232|A0A2Z5H232_9CAUD Uncharacterized protein OS=Mycobacterium phage Joselito OX=2234075 GN=65 PE=4 SV=1
MM1 pKa = 7.44TATKK5 pKa = 10.51LPYY8 pKa = 9.16LHH10 pKa = 6.99KK11 pKa = 10.35NARR14 pKa = 11.84SRR16 pKa = 11.84QITKK20 pKa = 10.37RR21 pKa = 11.84EE22 pKa = 3.83IRR24 pKa = 11.84DD25 pKa = 3.43VFADD29 pKa = 4.75EE30 pKa = 3.99ITRR33 pKa = 11.84GLDD36 pKa = 2.97RR37 pKa = 11.84RR38 pKa = 11.84IDD40 pKa = 3.28KK41 pKa = 10.78RR42 pKa = 11.84EE43 pKa = 3.78YY44 pKa = 9.93LRR46 pKa = 11.84KK47 pKa = 10.24VMPP50 pKa = 4.57

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16395

25

848

169.0

18.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.716 ± 0.309

0.927 ± 0.137

6.514 ± 0.182

7.228 ± 0.268

3.324 ± 0.159

8.015 ± 0.321

2.135 ± 0.169

5.111 ± 0.139

4.507 ± 0.255

8.009 ± 0.301

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.33 ± 0.106

3.373 ± 0.15

5.441 ± 0.266

3.617 ± 0.171

6.624 ± 0.39

5.154 ± 0.218

5.685 ± 0.244

7.307 ± 0.254

2.007 ± 0.155

2.977 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski