Shewanella loihica (strain ATCC BAA-1088 / PV-4)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella loihica

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3QDI1|A3QDI1_SHELP NAD-dependent protein deacylase OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) OX=323850 GN=cobB PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.35RR3 pKa = 11.84FKK5 pKa = 10.87CNRR8 pKa = 11.84LVRR11 pKa = 11.84ALGIVLISGASLYY24 pKa = 10.98GATITTANASFFPQKK39 pKa = 9.72MDD41 pKa = 3.53QACMAEE47 pKa = 3.91QAGFNLNCTANDD59 pKa = 3.38VRR61 pKa = 11.84VSKK64 pKa = 10.82VDD66 pKa = 3.92NIRR69 pKa = 11.84DD70 pKa = 3.88PNDD73 pKa = 3.3PNAKK77 pKa = 9.67VEE79 pKa = 4.38CNLGDD84 pKa = 3.88EE85 pKa = 4.65VTFIADD91 pKa = 3.36VTITTTANEE100 pKa = 4.21RR101 pKa = 11.84YY102 pKa = 9.41DD103 pKa = 3.72YY104 pKa = 11.16SVYY107 pKa = 10.55LPEE110 pKa = 5.19GNWSAQDD117 pKa = 3.51ANPNNEE123 pKa = 3.75CSILLGRR130 pKa = 11.84TDD132 pKa = 3.64GPGVDD137 pKa = 4.43LEE139 pKa = 4.7EE140 pKa = 5.52GLDD143 pKa = 3.49ACADD147 pKa = 3.22ISKK150 pKa = 10.48AAGYY154 pKa = 10.56NPTHH158 pKa = 6.66VYY160 pKa = 10.61ADD162 pKa = 3.55EE163 pKa = 5.13EE164 pKa = 4.19ITLFCRR170 pKa = 11.84DD171 pKa = 4.32DD172 pKa = 4.47DD173 pKa = 4.43NSGKK177 pKa = 10.63AEE179 pKa = 4.1FNYY182 pKa = 9.97CMAWHH187 pKa = 6.61NKK189 pKa = 7.86TGEE192 pKa = 4.36DD193 pKa = 4.59CSEE196 pKa = 5.07DD197 pKa = 4.09DD198 pKa = 3.92PAAPGTPSKK207 pKa = 10.59CRR209 pKa = 11.84CDD211 pKa = 3.75SFDD214 pKa = 3.34IDD216 pKa = 5.04VFIKK220 pKa = 10.54PNAPTITKK228 pKa = 9.79EE229 pKa = 4.26LIGTDD234 pKa = 3.1THH236 pKa = 6.18TEE238 pKa = 3.74PGGTYY243 pKa = 7.81TFKK246 pKa = 11.15LSFDD250 pKa = 3.72NPNAYY255 pKa = 8.9TSLFITDD262 pKa = 5.14LYY264 pKa = 11.6DD265 pKa = 4.43KK266 pKa = 10.79VDD268 pKa = 3.61EE269 pKa = 4.91GADD272 pKa = 3.52GSYY275 pKa = 9.25EE276 pKa = 4.15TMLDD280 pKa = 3.07LWNGVGAAGAADD292 pKa = 4.02GVYY295 pKa = 8.98LTASNCSQPANGGEE309 pKa = 4.12IVPSGSYY316 pKa = 9.99SCQFTVTIVDD326 pKa = 3.55RR327 pKa = 11.84DD328 pKa = 3.84LPDD331 pKa = 3.89DD332 pKa = 4.12QSPEE336 pKa = 4.09LYY338 pKa = 10.78NDD340 pKa = 3.95IIKK343 pKa = 10.59LALIDD348 pKa = 4.14KK349 pKa = 10.64NNDD352 pKa = 3.26PVVDD356 pKa = 4.31GGSCAAIGGVDD367 pKa = 4.19GDD369 pKa = 3.88HH370 pKa = 7.17CSNVLQVNVTNLPPSITVDD389 pKa = 3.18KK390 pKa = 11.02SAVPDD395 pKa = 3.54QVPEE399 pKa = 3.91SGAWVTYY406 pKa = 7.11TVRR409 pKa = 11.84VDD411 pKa = 3.35NTAADD416 pKa = 3.85YY417 pKa = 10.37DD418 pKa = 4.16SPLTLTYY425 pKa = 11.09LNDD428 pKa = 4.52DD429 pKa = 4.03KK430 pKa = 11.62FGDD433 pKa = 3.9LNGQGTCATGGTIAFGGYY451 pKa = 7.54YY452 pKa = 9.8EE453 pKa = 4.81CSFDD457 pKa = 4.62KK458 pKa = 10.93FISGTGAGSHH468 pKa = 6.16TNTATAKK475 pKa = 10.74AVDD478 pKa = 4.15DD479 pKa = 4.69EE480 pKa = 4.49NTEE483 pKa = 4.02AMNADD488 pKa = 3.69SATVIINDD496 pKa = 3.74IPSMITLEE504 pKa = 4.16KK505 pKa = 9.06TANPTSVLEE514 pKa = 4.39TGDD517 pKa = 3.54NPDD520 pKa = 3.1IYY522 pKa = 10.89RR523 pKa = 11.84DD524 pKa = 3.18VDD526 pKa = 3.87FTFWFSVDD534 pKa = 3.76DD535 pKa = 3.87QVGGQNTVDD544 pKa = 3.29SVTFATLTDD553 pKa = 4.94DD554 pKa = 3.48IFGVLTGDD562 pKa = 4.83CMVDD566 pKa = 3.31MKK568 pKa = 11.1NGAPIADD575 pKa = 3.79TPLAGFVLLPGEE587 pKa = 4.26NASCTITLQIQGYY600 pKa = 7.51RR601 pKa = 11.84TDD603 pKa = 2.69VHH605 pKa = 6.5TNVATIDD612 pKa = 3.72GTDD615 pKa = 3.37EE616 pKa = 4.77DD617 pKa = 4.48GQAVSDD623 pKa = 4.14SDD625 pKa = 3.93DD626 pKa = 3.44ATVTFTPAAPAVDD639 pKa = 3.71MDD641 pKa = 4.71FAATMLVVLGMHH653 pKa = 6.49NADD656 pKa = 3.49VNNANLTKK664 pKa = 9.92LTIGILDD671 pKa = 3.72VFAEE675 pKa = 4.37PDD677 pKa = 3.26TAGFKK682 pKa = 10.25IINGGGMYY690 pKa = 10.52NGTSYY695 pKa = 10.82GACGFNHH702 pKa = 6.88LFGYY706 pKa = 8.43TGSGTEE712 pKa = 4.4DD713 pKa = 3.71YY714 pKa = 10.35EE715 pKa = 4.54CAFTIEE721 pKa = 4.67LKK723 pKa = 10.68PGLEE727 pKa = 4.12NTDD730 pKa = 4.32PIAFLEE736 pKa = 4.26DD737 pKa = 3.37VVVEE741 pKa = 4.26LTNSQNDD748 pKa = 3.24KK749 pKa = 9.77STADD753 pKa = 3.22VSIQVGAIEE762 pKa = 3.89

Molecular weight: 81.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3Q8W2|A3Q8W2_SHELP Phosphoesterase PA-phosphatase related OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) OX=323850 GN=Shew_0037 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.3VIARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.35GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3855

0

3855

1309847

34

4836

339.8

37.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.724 ± 0.045

1.051 ± 0.016

5.608 ± 0.039

6.111 ± 0.039

3.839 ± 0.026

7.273 ± 0.038

2.238 ± 0.023

5.759 ± 0.032

4.872 ± 0.032

10.984 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.025

3.68 ± 0.036

4.086 ± 0.023

4.926 ± 0.039

4.775 ± 0.038

6.427 ± 0.039

5.041 ± 0.041

6.733 ± 0.038

1.212 ± 0.015

3.032 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski