Duck hepatitis B virus (isolate white Shanghai duck S31) (DHBV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Avihepadnavirus; Duck hepatitis B virus

Average proteome isoelectric point is 8.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P17195-2|HBSAG-2_HPBDW Isoform of P17195 Isoform S of Large envelope protein OS=Duck hepatitis B virus (isolate white Shanghai duck S31) OX=10440 GN=S PE=4 SV=3
MM1 pKa = 7.15GQQPAKK7 pKa = 10.78SMDD10 pKa = 2.92VRR12 pKa = 11.84RR13 pKa = 11.84IEE15 pKa = 4.69GGEE18 pKa = 3.94LLLNQLAGRR27 pKa = 11.84MIPKK31 pKa = 8.84GTVTWSGKK39 pKa = 10.16FPTIDD44 pKa = 3.24HH45 pKa = 6.83LLDD48 pKa = 3.59HH49 pKa = 6.24VQTMEE54 pKa = 4.49EE55 pKa = 4.67VNTLQQQGAWPAGAGRR71 pKa = 11.84RR72 pKa = 11.84LGLTNPAPQEE82 pKa = 3.95PPQPQWTPEE91 pKa = 3.83EE92 pKa = 4.01DD93 pKa = 3.23QKK95 pKa = 11.68ARR97 pKa = 11.84EE98 pKa = 3.95AFRR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 8.96QEE105 pKa = 3.91EE106 pKa = 4.36RR107 pKa = 11.84PPEE110 pKa = 4.18TTTIPPTSPTPWKK123 pKa = 9.68LQPGDD128 pKa = 4.75DD129 pKa = 3.94PLLEE133 pKa = 4.35NKK135 pKa = 10.1SLLEE139 pKa = 3.89THH141 pKa = 7.35PLYY144 pKa = 10.71QNPEE148 pKa = 3.83PAVPVIKK155 pKa = 9.87TPPLRR160 pKa = 11.84KK161 pKa = 9.58KK162 pKa = 10.67KK163 pKa = 9.55MAGTFGGILAGLIGLLVGFFLLIKK187 pKa = 9.98ILEE190 pKa = 4.06ILRR193 pKa = 11.84RR194 pKa = 11.84LDD196 pKa = 3.1WWWISLSSPKK206 pKa = 10.95GKK208 pKa = 8.31MQCAFQDD215 pKa = 3.1TGAQISPHH223 pKa = 5.53YY224 pKa = 10.76AGFCPWGCPGFLWTYY239 pKa = 10.39LRR241 pKa = 11.84LFIIFLLILLVAAGLLYY258 pKa = 10.12LTDD261 pKa = 3.47NMSIILGKK269 pKa = 9.45LQWEE273 pKa = 4.59SVSALFSSISSLLPSDD289 pKa = 3.65QKK291 pKa = 11.76SLVALMFGLLLIWMTSSSATQTLVTLTQLATLSALFYY328 pKa = 11.17KK329 pKa = 10.71NN330 pKa = 3.18

Molecular weight: 36.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P17195|HBSAG_HPBDW Large envelope protein OS=Duck hepatitis B virus (isolate white Shanghai duck S31) OX=10440 GN=S PE=3 SV=3
MM1 pKa = 7.45PQPLKK6 pKa = 10.84QSLDD10 pKa = 3.26QSKK13 pKa = 8.61WLRR16 pKa = 11.84EE17 pKa = 4.17AEE19 pKa = 3.91KK20 pKa = 10.53HH21 pKa = 6.08LRR23 pKa = 11.84EE24 pKa = 4.61LEE26 pKa = 4.05NLVDD30 pKa = 4.46SNLEE34 pKa = 3.92EE35 pKa = 4.59EE36 pKa = 4.45KK37 pKa = 10.9LKK39 pKa = 10.68PHH41 pKa = 6.94LSMGEE46 pKa = 4.14DD47 pKa = 3.32VQSPGKK53 pKa = 10.64GEE55 pKa = 3.85PLHH58 pKa = 6.29PNVRR62 pKa = 11.84APLSHH67 pKa = 6.3VVRR70 pKa = 11.84AATIDD75 pKa = 3.69LPRR78 pKa = 11.84LGNKK82 pKa = 9.66LPAKK86 pKa = 9.73HH87 pKa = 6.56HH88 pKa = 6.15LGKK91 pKa = 10.87LSGLYY96 pKa = 9.38QMKK99 pKa = 9.7GCSFNPEE106 pKa = 3.6WKK108 pKa = 10.68VPDD111 pKa = 3.45ISDD114 pKa = 3.37THH116 pKa = 7.37FDD118 pKa = 3.41LQVINEE124 pKa = 4.23CPSRR128 pKa = 11.84NWKK131 pKa = 9.93YY132 pKa = 8.85LTPAKK137 pKa = 9.91FWPKK141 pKa = 9.85SISYY145 pKa = 9.65FPVQAGVKK153 pKa = 9.77AKK155 pKa = 10.91YY156 pKa = 8.9PDD158 pKa = 3.32NVMQHH163 pKa = 5.26EE164 pKa = 4.88AIVGKK169 pKa = 9.48YY170 pKa = 9.86LNRR173 pKa = 11.84LYY175 pKa = 10.71EE176 pKa = 3.99AGILYY181 pKa = 10.13KK182 pKa = 10.39RR183 pKa = 11.84ISKK186 pKa = 10.27HH187 pKa = 5.64LVTFKK192 pKa = 10.65GKK194 pKa = 9.13PYY196 pKa = 9.79HH197 pKa = 6.94WEE199 pKa = 3.47LQYY202 pKa = 11.11LVKK205 pKa = 10.33QHH207 pKa = 5.66QVPDD211 pKa = 3.49GTTTCKK217 pKa = 10.36INGRR221 pKa = 11.84EE222 pKa = 3.8EE223 pKa = 3.7NRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84RR228 pKa = 11.84APAKK232 pKa = 10.28SISRR236 pKa = 11.84PHH238 pKa = 5.44DD239 pKa = 3.36TEE241 pKa = 4.9RR242 pKa = 11.84NCHH245 pKa = 5.08MVGQVSNNRR254 pKa = 11.84SPIRR258 pKa = 11.84SCANNGGGKK267 pKa = 10.13YY268 pKa = 10.29SSTTRR273 pKa = 11.84RR274 pKa = 11.84MACWGGQTIGVNQSCSPRR292 pKa = 11.84TSAASVDD299 pKa = 2.99AGRR302 pKa = 11.84RR303 pKa = 11.84SKK305 pKa = 10.81SSRR308 pKa = 11.84GLSSLSRR315 pKa = 11.84RR316 pKa = 11.84EE317 pKa = 3.84TTGNHH322 pKa = 4.29HH323 pKa = 7.06HH324 pKa = 6.26STNVTNSVEE333 pKa = 3.67AATRR337 pKa = 11.84GRR339 pKa = 11.84SPAGKK344 pKa = 9.25QVSARR349 pKa = 11.84DD350 pKa = 3.74TSSLPEE356 pKa = 4.27SGASRR361 pKa = 11.84ACDD364 pKa = 3.51KK365 pKa = 11.14NSSPQKK371 pKa = 10.55EE372 pKa = 4.06EE373 pKa = 3.82NGWYY377 pKa = 10.42LRR379 pKa = 11.84GNTSWPNRR387 pKa = 11.84ITGRR391 pKa = 11.84LFLVDD396 pKa = 3.43KK397 pKa = 10.74NSRR400 pKa = 11.84NTTEE404 pKa = 3.6ARR406 pKa = 11.84LVVDD410 pKa = 5.36FSQFSKK416 pKa = 10.78GKK418 pKa = 9.03NAMRR422 pKa = 11.84FPRR425 pKa = 11.84YY426 pKa = 8.48WSPNLSTLRR435 pKa = 11.84RR436 pKa = 11.84ILPVGMPRR444 pKa = 11.84ISLDD448 pKa = 3.21LSQAFYY454 pKa = 10.9HH455 pKa = 6.6LPLNPASSSRR465 pKa = 11.84LAVSDD470 pKa = 3.95GQHH473 pKa = 4.67VYY475 pKa = 10.42YY476 pKa = 10.43FRR478 pKa = 11.84KK479 pKa = 10.17APMGVGLSPFLLHH492 pKa = 7.37LFTTALGSEE501 pKa = 3.97IARR504 pKa = 11.84RR505 pKa = 11.84FNVWTFTYY513 pKa = 9.89MDD515 pKa = 5.26DD516 pKa = 5.74FLLCHH521 pKa = 6.51PNARR525 pKa = 11.84HH526 pKa = 6.01LNSISHH532 pKa = 6.54AVCTFLQEE540 pKa = 3.68LGIRR544 pKa = 11.84INFDD548 pKa = 2.79KK549 pKa = 8.52TTPSPVNDD557 pKa = 3.02IRR559 pKa = 11.84FLGYY563 pKa = 10.64QIDD566 pKa = 3.86QKK568 pKa = 10.9FMRR571 pKa = 11.84IEE573 pKa = 3.66EE574 pKa = 4.39SRR576 pKa = 11.84WKK578 pKa = 10.33EE579 pKa = 3.38LRR581 pKa = 11.84TVIKK585 pKa = 9.99KK586 pKa = 10.14IKK588 pKa = 9.72IGAWYY593 pKa = 9.18DD594 pKa = 3.2WKK596 pKa = 10.91CIQRR600 pKa = 11.84FVGHH604 pKa = 6.96LNFVLPFTKK613 pKa = 10.62GNIEE617 pKa = 4.08MLKK620 pKa = 10.42PMYY623 pKa = 10.41AAITNKK629 pKa = 9.7VNFSFSSAYY638 pKa = 8.34RR639 pKa = 11.84TLLYY643 pKa = 10.37KK644 pKa = 10.16LTMGVCKK651 pKa = 9.82LTIRR655 pKa = 11.84PKK657 pKa = 10.8SSVPLPRR664 pKa = 11.84VATDD668 pKa = 3.36ATPTHH673 pKa = 6.4GAISHH678 pKa = 5.63ITGGSAVLAFSKK690 pKa = 10.75VRR692 pKa = 11.84DD693 pKa = 3.36IHH695 pKa = 7.3IQEE698 pKa = 4.6LLMVCLAKK706 pKa = 10.61IMIKK710 pKa = 9.85PRR712 pKa = 11.84CILSDD717 pKa = 3.64STFVCYY723 pKa = 10.1KK724 pKa = 10.25RR725 pKa = 11.84YY726 pKa = 8.32HH727 pKa = 5.26TLPWHH732 pKa = 6.39FAMLAKK738 pKa = 10.32QLLSPIQLYY747 pKa = 8.05FVPSKK752 pKa = 10.83YY753 pKa = 10.6NPADD757 pKa = 3.51GPSRR761 pKa = 11.84HH762 pKa = 6.27KK763 pKa = 10.97PPDD766 pKa = 3.32WTALTYY772 pKa = 10.44TPLSKK777 pKa = 10.42AIYY780 pKa = 9.36IPHH783 pKa = 7.04RR784 pKa = 11.84LCGTT788 pKa = 3.88

Molecular weight: 89.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1852

167

788

370.4

41.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.803 ± 0.424

1.458 ± 0.317

3.942 ± 0.46

4.428 ± 0.558

3.726 ± 0.347

5.886 ± 0.559

2.97 ± 0.554

5.832 ± 0.536

5.724 ± 0.569

11.177 ± 1.619

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.944 ± 0.323

3.294 ± 0.809

7.073 ± 0.551

3.834 ± 0.569

7.181 ± 1.111

8.315 ± 0.547

6.317 ± 0.276

4.536 ± 0.482

2.484 ± 0.341

3.078 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski