Acholeplasma laidlawii (strain PG-8A)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1380 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A9NHW7|A9NHW7_ACHLI [Ribosomal protein S18]-alanine N-acetyltransferase OS=Acholeplasma laidlawii (strain PG-8A) OX=441768 GN=rimI PE=3 SV=1
MM1 pKa = 7.47 KK2 pKa = 9.9 KK3 pKa = 9.95 ISIIFTLLMTLVLIACTEE21 pKa = 3.85 LTAYY25 pKa = 9.91 KK26 pKa = 10.09 IVFEE30 pKa = 4.59 TNGGNDD36 pKa = 2.79 IQAIEE41 pKa = 4.21 FEE43 pKa = 4.34 KK44 pKa = 10.72 SYY46 pKa = 11.61 ALAEE50 pKa = 4.11 IEE52 pKa = 4.27 KK53 pKa = 10.31 VVPEE57 pKa = 3.91 KK58 pKa = 10.72 SGYY61 pKa = 7.79 TFNSWYY67 pKa = 10.7 LEE69 pKa = 3.79 SDD71 pKa = 3.44 LNEE74 pKa = 4.13 GSKK77 pKa = 10.93 LSTDD81 pKa = 2.88 ITSSVTLYY89 pKa = 11.05 AKK91 pKa = 8.33 WTITEE96 pKa = 4.08 YY97 pKa = 10.75 TITLHH102 pKa = 7.04 LDD104 pKa = 3.26 GGVIHH109 pKa = 7.14 PEE111 pKa = 3.86 QVSKK115 pKa = 9.14 FTILDD120 pKa = 3.73 EE121 pKa = 4.66 VNLLTPTKK129 pKa = 10.38 EE130 pKa = 4.01 GFKK133 pKa = 10.73 FLGWSISNTEE143 pKa = 3.56 ASFVDD148 pKa = 3.72 KK149 pKa = 11.52 VEE151 pKa = 4.3 VGSTEE156 pKa = 3.96 HH157 pKa = 6.74 KK158 pKa = 10.47 SFYY161 pKa = 11.1 AKK163 pKa = 9.74 WEE165 pKa = 3.91 NLGEE169 pKa = 4.19 VEE171 pKa = 4.13 TFEE174 pKa = 5.96 ISFKK178 pKa = 10.6 NHH180 pKa = 6.49 DD181 pKa = 4.0 NAVLQTVEE189 pKa = 4.28 VASGVVPAYY198 pKa = 9.42 TGQTPTKK205 pKa = 9.49 EE206 pKa = 3.75 ATLTHH211 pKa = 5.91 TFEE214 pKa = 4.04 FTGWDD219 pKa = 3.5 KK220 pKa = 11.59 AVVAATADD228 pKa = 3.29 IVYY231 pKa = 9.59 VAQFKK236 pKa = 9.46 EE237 pKa = 4.37 VPITSGTTFNPALLNSIFGLDD258 pKa = 3.55 VYY260 pKa = 11.52 ALIPEE265 pKa = 4.69 IVTSDD270 pKa = 3.75 YY271 pKa = 11.51 EE272 pKa = 4.73 VIDD275 pKa = 3.8 NSNDD279 pKa = 3.19 LFNDD283 pKa = 3.63 VYY285 pKa = 11.08 IDD287 pKa = 3.61 VFDD290 pKa = 3.72 WTEE293 pKa = 4.09 SDD295 pKa = 3.94 LMAYY299 pKa = 9.56 DD300 pKa = 4.38 ALLEE304 pKa = 4.14 NLLTYY309 pKa = 10.56 DD310 pKa = 4.18 ALEE313 pKa = 4.2 DD314 pKa = 3.94 AYY316 pKa = 10.07 ILGEE320 pKa = 4.02 LFIYY324 pKa = 10.08 LYY326 pKa = 10.9 ADD328 pKa = 3.84 DD329 pKa = 5.66 EE330 pKa = 4.56 IVPGSIIYY338 pKa = 10.16 GIGIYY343 pKa = 9.91 QYY345 pKa = 11.3 LEE347 pKa = 4.6 DD348 pKa = 4.66 EE349 pKa = 4.75 TPVDD353 pKa = 3.53 PVDD356 pKa = 3.58 PVGAPFDD363 pKa = 4.0 KK364 pKa = 11.19 DD365 pKa = 3.39 EE366 pKa = 5.08 LNGIFGFDD374 pKa = 3.25 IYY376 pKa = 11.55 ALLPAIISEE385 pKa = 4.46 DD386 pKa = 3.58 VLITDD391 pKa = 5.33 LSDD394 pKa = 2.97 EE395 pKa = 4.67 TYY397 pKa = 10.39 IEE399 pKa = 4.35 VYY401 pKa = 10.92 VDD403 pKa = 3.17 IFTWLDD409 pKa = 3.12 ADD411 pKa = 3.85 ADD413 pKa = 4.07 AYY415 pKa = 10.84 DD416 pKa = 4.14 ALLSGSLAYY425 pKa = 10.26 DD426 pKa = 3.55 ATEE429 pKa = 4.08 DD430 pKa = 3.6 AYY432 pKa = 10.22 KK433 pKa = 10.81 LGDD436 pKa = 3.41 YY437 pKa = 9.67 FAYY440 pKa = 9.82 IFIDD444 pKa = 3.51 EE445 pKa = 4.22 EE446 pKa = 4.91 TYY448 pKa = 10.63 PGLTVFGLAIYY459 pKa = 10.0 GDD461 pKa = 3.92 KK462 pKa = 11.24 AGTTPVDD469 pKa = 3.29 PVDD472 pKa = 3.62 PVGAPFDD479 pKa = 4.0 KK480 pKa = 11.19 DD481 pKa = 3.39 EE482 pKa = 5.08 LNGIFGFDD490 pKa = 3.25 IYY492 pKa = 11.51 ALLPAIVSEE501 pKa = 4.61 DD502 pKa = 3.57 VLITDD507 pKa = 5.26 LSDD510 pKa = 3.1 EE511 pKa = 4.82 TYY513 pKa = 10.73 VEE515 pKa = 4.46 VYY517 pKa = 11.01 VDD519 pKa = 3.41 IFTWLDD525 pKa = 3.12 ADD527 pKa = 3.85 ADD529 pKa = 4.07 AYY531 pKa = 10.84 DD532 pKa = 4.14 ALLSGSLAYY541 pKa = 10.26 DD542 pKa = 3.55 ATEE545 pKa = 4.08 DD546 pKa = 3.6 AYY548 pKa = 10.22 KK549 pKa = 10.81 LGDD552 pKa = 3.41 YY553 pKa = 9.67 FAYY556 pKa = 9.82 IFIDD560 pKa = 3.51 EE561 pKa = 4.22 EE562 pKa = 4.91 TYY564 pKa = 10.63 PGLTVFGLAIYY575 pKa = 10.0 GDD577 pKa = 3.92 KK578 pKa = 11.24 AGTTPVDD585 pKa = 3.45 PVEE588 pKa = 4.22 PEE590 pKa = 3.18 IGEE593 pKa = 4.27 YY594 pKa = 11.06 YY595 pKa = 10.59 SFNVQDD601 pKa = 3.68 TTSTLDD607 pKa = 3.05 GSYY610 pKa = 10.73 RR611 pKa = 11.84 NNIDD615 pKa = 3.1 VTLNFANNTNKK626 pKa = 10.01 VIVKK630 pKa = 9.87 ASHH633 pKa = 6.64 IANITQTAPGGLSLGKK649 pKa = 10.0 IFAANVSGNANPTVYY664 pKa = 10.91 LEE666 pKa = 4.09 IDD668 pKa = 3.2 ALGNLIDD675 pKa = 3.84 TMSFEE680 pKa = 4.19 IQGRR684 pKa = 11.84 TGFSPNLAGAKK695 pKa = 9.8 LQVFNNGVWTDD706 pKa = 3.41 LAGGNFYY713 pKa = 11.08 SQIASSKK720 pKa = 8.41 TLITISGINASKK732 pKa = 10.35 FRR734 pKa = 11.84 LLFQGTGATSNGGQFMIFNVNLLTGNAPAPVYY766 pKa = 9.29 EE767 pKa = 4.3 LWSDD771 pKa = 3.69 VVTDD775 pKa = 4.94 LEE777 pKa = 5.02 AKK779 pKa = 10.42 FDD781 pKa = 4.18 DD782 pKa = 4.96 LDD784 pKa = 4.3 FNTYY788 pKa = 9.55 MPDD791 pKa = 3.81 FADD794 pKa = 3.65 LTNLKK799 pKa = 7.99 VTKK802 pKa = 10.68 VSDD805 pKa = 3.22 KK806 pKa = 10.31 SFKK809 pKa = 10.77 VVGSTTLDD817 pKa = 3.3 VNTLYY822 pKa = 10.58 TSYY825 pKa = 11.33 INLILNKK832 pKa = 10.1 SFEE835 pKa = 4.55 KK836 pKa = 10.89 NDD838 pKa = 3.65 DD839 pKa = 3.51 LSLVRR844 pKa = 11.84 GHH846 pKa = 6.97 DD847 pKa = 3.47 VYY849 pKa = 11.73 VYY851 pKa = 10.56 VVNDD855 pKa = 3.73 DD856 pKa = 3.84 LAYY859 pKa = 10.74 AMYY862 pKa = 9.92 IIKK865 pKa = 8.86 GTEE868 pKa = 3.74 SLEE871 pKa = 3.99 VYY873 pKa = 9.91 IYY875 pKa = 10.83 QFDD878 pKa = 3.7 AVMDD882 pKa = 4.24 DD883 pKa = 3.95 VVLEE887 pKa = 4.28 TLSKK891 pKa = 9.59 RR892 pKa = 11.84 QSINEE897 pKa = 4.0 YY898 pKa = 9.69 EE899 pKa = 4.21 VSQFGMSGLPSTGTYY914 pKa = 10.42 DD915 pKa = 3.51 VLVVPVEE922 pKa = 4.0 IQNVPFEE929 pKa = 4.43 ASYY932 pKa = 8.51 KK933 pKa = 9.13 TKK935 pKa = 10.5 LDD937 pKa = 3.28 KK938 pKa = 11.34 VFNGTSLDD946 pKa = 3.88 TGWEE950 pKa = 4.49 SVSSYY955 pKa = 10.64 YY956 pKa = 10.64 YY957 pKa = 10.36 KK958 pKa = 10.85 SSFGLLDD965 pKa = 4.27 LNFDD969 pKa = 3.84 ILDD972 pKa = 3.48 KK973 pKa = 11.13 HH974 pKa = 5.19 VTSNVKK980 pKa = 10.27 AFYY983 pKa = 9.81 EE984 pKa = 4.6 GKK986 pKa = 10.43 GQDD989 pKa = 3.3 GDD991 pKa = 3.54 QYY993 pKa = 11.78 AILDD997 pKa = 4.16 ALTALDD1003 pKa = 3.71 STIDD1007 pKa = 3.62 FSKK1010 pKa = 11.1 YY1011 pKa = 10.19 DD1012 pKa = 3.71 SNNDD1016 pKa = 3.22 GVIDD1020 pKa = 4.37 SIIFIYY1026 pKa = 9.26 STDD1029 pKa = 3.24 YY1030 pKa = 11.43 NYY1032 pKa = 11.25 DD1033 pKa = 3.36 VDD1035 pKa = 4.85 PWWAWVYY1042 pKa = 10.77 VSNPDD1047 pKa = 3.0 IVGSVSEE1054 pKa = 4.31 LDD1056 pKa = 3.53 GKK1058 pKa = 10.42 NFEE1061 pKa = 4.51 YY1062 pKa = 10.9 YY1063 pKa = 10.13 FWASYY1068 pKa = 11.68 DD1069 pKa = 3.6 FMNDD1073 pKa = 3.28 ALPGNSDD1080 pKa = 4.96 LILNSEE1086 pKa = 4.5 TYY1088 pKa = 9.82 IHH1090 pKa = 6.7 EE1091 pKa = 5.01 LGHH1094 pKa = 7.61 LMGIVDD1100 pKa = 4.49 FYY1102 pKa = 10.84 PYY1104 pKa = 10.45 EE1105 pKa = 4.38 GNNQYY1110 pKa = 11.24 GPMGGFDD1117 pKa = 3.37 MMDD1120 pKa = 3.84 YY1121 pKa = 10.91 NAGDD1125 pKa = 3.51 HH1126 pKa = 6.8 GPFNKK1131 pKa = 10.17 LVFGWLQPLVAQKK1144 pKa = 9.29 GTYY1147 pKa = 9.1 QVTLDD1152 pKa = 4.19 SYY1154 pKa = 11.31 STDD1157 pKa = 3.3 TDD1159 pKa = 3.84 GLNSTLLIPFNSSDD1173 pKa = 4.2 LNDD1176 pKa = 3.32 GNAFDD1181 pKa = 4.98 EE1182 pKa = 4.47 YY1183 pKa = 10.66 LLVMFYY1189 pKa = 10.35 TPNGLYY1195 pKa = 9.93 SAHH1198 pKa = 6.86 SGLEE1202 pKa = 4.11 YY1203 pKa = 10.37 IPSNAGIVVYY1213 pKa = 10.52 HH1214 pKa = 6.56 IDD1216 pKa = 4.49 ARR1218 pKa = 11.84 LTSNPVFWGEE1228 pKa = 3.67 YY1229 pKa = 8.97 FRR1231 pKa = 11.84 NNNEE1235 pKa = 4.02 GASSFINQILEE1246 pKa = 3.86 ADD1248 pKa = 4.17 KK1249 pKa = 11.52 NNSIPGNGSIKK1260 pKa = 10.56 QSDD1263 pKa = 4.4 LLTSGTLNLNTYY1275 pKa = 8.9 SWNQGGKK1282 pKa = 9.45 INVSLTVASLFNNSSEE1298 pKa = 4.1 NVTLTVSVAA1307 pKa = 3.15
Molecular weight: 144.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.795
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.19
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.961
Patrickios 0.477
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|A9NE66|A9NE66_ACHLI IS-10 transposase OS=Acholeplasma laidlawii (strain PG-8A) OX=441768 GN=ACL_0003 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.95 QPSKK9 pKa = 9.3 IKK11 pKa = 9.77 HH12 pKa = 4.29 QRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.25 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.24 GRR39 pKa = 11.84 QSLTVV44 pKa = 3.17
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1380
0
1380
451261
25
5552
327.0
37.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.75 ± 0.063
0.351 ± 0.012
6.011 ± 0.046
6.355 ± 0.05
4.843 ± 0.049
6.006 ± 0.079
2.089 ± 0.036
8.963 ± 0.064
7.82 ± 0.089
10.006 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.55 ± 0.031
5.534 ± 0.071
3.045 ± 0.032
3.106 ± 0.037
3.336 ± 0.048
6.139 ± 0.044
5.922 ± 0.075
6.846 ± 0.055
0.737 ± 0.023
4.591 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here