Pseudomonas phage MPK7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; Phikmvvirus; Pseudomonas virus MPK7

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4SZG7|S4SZG7_9CAUD DNA maturase B OS=Pseudomonas phage MPK7 OX=1225790 GN=MPK7_48 PE=4 SV=1
MM1 pKa = 7.52ARR3 pKa = 11.84FKK5 pKa = 11.05NPEE8 pKa = 4.31TIHH11 pKa = 6.06VADD14 pKa = 3.82GVEE17 pKa = 4.42AVFSLDD23 pKa = 3.36FPFLRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 4.09DD31 pKa = 3.59VFVQVDD37 pKa = 4.24KK38 pKa = 11.36ILVTDD43 pKa = 3.85YY44 pKa = 10.92TWVDD48 pKa = 3.33DD49 pKa = 4.26TNIQLAVVPKK59 pKa = 9.93KK60 pKa = 10.33DD61 pKa = 3.18QEE63 pKa = 4.01VRR65 pKa = 11.84IFRR68 pKa = 11.84DD69 pKa = 3.52TPAQVPDD76 pKa = 3.7TQFSQGIPFLPRR88 pKa = 11.84YY89 pKa = 9.44IDD91 pKa = 3.78ANNKK95 pKa = 7.73QLLYY99 pKa = 10.69AVQEE103 pKa = 4.83GINTANLALDD113 pKa = 4.02GVLDD117 pKa = 5.11AIRR120 pKa = 11.84IAEE123 pKa = 4.12EE124 pKa = 3.63ARR126 pKa = 11.84RR127 pKa = 11.84LAQEE131 pKa = 4.42ALDD134 pKa = 4.19AANEE138 pKa = 4.02ALRR141 pKa = 11.84RR142 pKa = 11.84ALGFAEE148 pKa = 3.77IRR150 pKa = 11.84TVTEE154 pKa = 4.95DD155 pKa = 3.95SDD157 pKa = 4.62IDD159 pKa = 4.09PSWRR163 pKa = 11.84GYY165 pKa = 7.36WNRR168 pKa = 11.84CITTDD173 pKa = 3.65KK174 pKa = 11.24SLTLTMQMEE183 pKa = 4.91DD184 pKa = 3.93PDD186 pKa = 4.2EE187 pKa = 4.22PWIEE191 pKa = 3.83FSEE194 pKa = 4.42VHH196 pKa = 6.29FEE198 pKa = 3.83QAGIRR203 pKa = 11.84DD204 pKa = 4.21LNIVAGPGVTINRR217 pKa = 11.84LQNTTMQLYY226 pKa = 10.33GEE228 pKa = 4.7NGVCTLKK235 pKa = 10.78RR236 pKa = 11.84IGPNHH241 pKa = 6.92WIIFGAMEE249 pKa = 4.85DD250 pKa = 3.86EE251 pKa = 4.44

Molecular weight: 28.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4SY57|S4SY57_9CAUD Uncharacterized protein OS=Pseudomonas phage MPK7 OX=1225790 GN=MPK7_12 PE=4 SV=1
MM1 pKa = 7.71SSRR4 pKa = 11.84DD5 pKa = 3.56PYY7 pKa = 10.8RR8 pKa = 11.84IGHH11 pKa = 6.13RR12 pKa = 11.84VGLVNYY18 pKa = 9.6SDD20 pKa = 4.68RR21 pKa = 11.84YY22 pKa = 10.32LGADD26 pKa = 3.1AAGTKK31 pKa = 10.38GIIEE35 pKa = 5.36AITRR39 pKa = 11.84PSRR42 pKa = 11.84CMTVYY47 pKa = 10.38HH48 pKa = 5.6VRR50 pKa = 11.84CEE52 pKa = 3.71RR53 pKa = 11.84TLRR56 pKa = 11.84LIEE59 pKa = 4.13AEE61 pKa = 3.77ARR63 pKa = 11.84NVRR66 pKa = 11.84FIRR69 pKa = 11.84QLPTRR74 pKa = 11.84KK75 pKa = 9.78

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13370

41

1337

252.3

27.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.249 ± 0.582

0.942 ± 0.17

5.886 ± 0.211

6.238 ± 0.237

3.224 ± 0.159

7.996 ± 0.329

2.229 ± 0.177

4.316 ± 0.215

4.615 ± 0.271

8.766 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.022 ± 0.118

3.598 ± 0.214

4.645 ± 0.342

4.592 ± 0.302

7.105 ± 0.279

4.869 ± 0.198

5.423 ± 0.248

6.776 ± 0.277

1.578 ± 0.121

2.932 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski