Carrot yellow leaf virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GFK6|C6GFK6_9CLOS p25 OS=Carrot yellow leaf virus OX=656190 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.13LYY4 pKa = 10.36ISIYY8 pKa = 9.98EE9 pKa = 4.11YY10 pKa = 10.16IALVSEE16 pKa = 4.68ISRR19 pKa = 11.84LLDD22 pKa = 3.42LVSEE26 pKa = 4.64CVSGVTYY33 pKa = 10.83DD34 pKa = 2.99KK35 pKa = 11.01FKK37 pKa = 9.62EE38 pKa = 3.7FRR40 pKa = 11.84YY41 pKa = 10.13RR42 pKa = 11.84FVHH45 pKa = 5.87LTMLIPSLKK54 pKa = 10.22SDD56 pKa = 4.19LNDD59 pKa = 3.4SLRR62 pKa = 11.84DD63 pKa = 3.51EE64 pKa = 4.73GFSMQSEE71 pKa = 4.35KK72 pKa = 10.89KK73 pKa = 8.2EE74 pKa = 3.77QLVLFEE80 pKa = 4.69KK81 pKa = 10.08TCSDD85 pKa = 3.58VQSKK89 pKa = 8.99LRR91 pKa = 11.84RR92 pKa = 11.84MIVQSYY98 pKa = 10.01SISSPLDD105 pKa = 3.2LVVFFCKK112 pKa = 9.75KK113 pKa = 9.32HH114 pKa = 6.94YY115 pKa = 10.27EE116 pKa = 4.07MRR118 pKa = 11.84CCDD121 pKa = 3.99YY122 pKa = 10.74STIMHH127 pKa = 6.96DD128 pKa = 4.0KK129 pKa = 10.79VKK131 pKa = 10.1PSCDD135 pKa = 2.92AVLRR139 pKa = 11.84DD140 pKa = 3.3ISNTFGVDD148 pKa = 2.83VSPRR152 pKa = 11.84SFEE155 pKa = 3.95NGGFLTLKK163 pKa = 9.55DD164 pKa = 3.3TSFRR168 pKa = 11.84HH169 pKa = 5.71LFKK172 pKa = 11.05DD173 pKa = 3.54CFGVGEE179 pKa = 4.11EE180 pKa = 4.34EE181 pKa = 4.29IRR183 pKa = 11.84RR184 pKa = 11.84LLNEE188 pKa = 4.19RR189 pKa = 11.84EE190 pKa = 4.29TGSSLFSVEE199 pKa = 4.26PYY201 pKa = 8.7VPKK204 pKa = 10.63YY205 pKa = 10.02VSEE208 pKa = 4.21GAEE211 pKa = 3.85SHH213 pKa = 6.05II214 pKa = 4.85

Molecular weight: 24.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GFK4|C6GFK4_9CLOS p26 OS=Carrot yellow leaf virus OX=656190 PE=4 SV=1
MM1 pKa = 7.43SKK3 pKa = 10.61EE4 pKa = 3.93LMIVSPDD11 pKa = 3.3GSLEE15 pKa = 3.94KK16 pKa = 10.75ADD18 pKa = 3.7VSKK21 pKa = 10.96LAIATRR27 pKa = 11.84EE28 pKa = 4.0SMQAAFSALDD38 pKa = 3.75LSSVSGTSDD47 pKa = 2.98SCLSDD52 pKa = 4.03SEE54 pKa = 4.28LTEE57 pKa = 4.12AATKK61 pKa = 10.9VNTEE65 pKa = 4.09LKK67 pKa = 10.33KK68 pKa = 9.09ITKK71 pKa = 10.42GEE73 pKa = 4.54DD74 pKa = 2.72IDD76 pKa = 4.29MPSHH80 pKa = 6.24FAALIARR87 pKa = 11.84AATIGTSLSAVYY99 pKa = 10.22RR100 pKa = 11.84NQQTYY105 pKa = 10.18SIRR108 pKa = 11.84GKK110 pKa = 10.69GKK112 pKa = 8.46YY113 pKa = 6.27TVKK116 pKa = 10.45DD117 pKa = 3.59AEE119 pKa = 4.26IFPYY123 pKa = 10.35IIDD126 pKa = 3.36ITAKK130 pKa = 9.41YY131 pKa = 9.44GKK133 pKa = 9.42PNGLRR138 pKa = 11.84AFFASLEE145 pKa = 4.09NAFLVIAKK153 pKa = 7.54MKK155 pKa = 9.55PQLFEE160 pKa = 4.32SRR162 pKa = 11.84VATRR166 pKa = 11.84RR167 pKa = 11.84GTPKK171 pKa = 10.35EE172 pKa = 3.84KK173 pKa = 10.61GYY175 pKa = 10.52LATDD179 pKa = 4.53FLSGASPILVDD190 pKa = 4.09QEE192 pKa = 4.12RR193 pKa = 11.84AILNSASSYY202 pKa = 11.03ALDD205 pKa = 3.62RR206 pKa = 11.84AASKK210 pKa = 10.48KK211 pKa = 10.22KK212 pKa = 10.47NSGLVSLYY220 pKa = 10.77DD221 pKa = 3.46YY222 pKa = 11.41GRR224 pKa = 11.84YY225 pKa = 9.29DD226 pKa = 3.02

Molecular weight: 24.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

5163

60

2431

516.3

58.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.043 ± 0.568

2.247 ± 0.183

5.404 ± 0.175

5.559 ± 0.261

5.907 ± 0.436

5.075 ± 0.408

2.305 ± 0.273

5.539 ± 0.32

6.488 ± 0.322

9.607 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.146

3.68 ± 0.249

4.009 ± 0.247

2.382 ± 0.334

5.559 ± 0.555

11.059 ± 0.318

5.617 ± 0.325

7.011 ± 0.376

0.542 ± 0.179

4.048 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski