Proteome-pI 2.0 - Proteome Isoelectric Point Database

Shahe narna-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.4

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KIM0|A0A1L3KIM0_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 1 OX=1923429 PE=4 SV=1

MM1 pKa = 7.79EE2 pKa = 4.51SQQRR6 pKa = 11.84SLDD9 pKa = 3.74VVCQNLGQLRR19 pKa = 11.84KK20 pKa = 9.63RR21 pKa = 11.84CTGEE25 pKa = 4.63DD26 pKa = 2.99IITFVDD32 pKa = 3.63CWYY35 pKa = 8.87STTVHH40 pKa = 6.02EE41 pKa = 5.52VRR43 pKa = 11.84SPWISKK49 pKa = 9.34LRR51 pKa = 11.84PIDD54 pKa = 3.37VFHH57 pKa = 7.1YY58 pKa = 7.38YY59 pKa = 10.31TLVKK63 pKa = 9.53VAKK66 pKa = 9.98IGPTLDD72 pKa = 3.22THH74 pKa = 6.32PLEE77 pKa = 4.99DD78 pKa = 3.06VYY80 pKa = 11.39KK81 pKa = 9.1AVKK84 pKa = 10.04IISQYY89 pKa = 10.19TEE91 pKa = 3.77MTHH94 pKa = 6.84SYY96 pKa = 10.97SKK98 pKa = 11.11LLDD101 pKa = 3.56ASGYY105 pKa = 8.67GEE107 pKa = 5.04KK108 pKa = 9.75IRR110 pKa = 11.84QEE112 pKa = 4.08FSDD115 pKa = 3.88TLLSAFDD122 pKa = 4.4KK123 pKa = 11.23DD124 pKa = 3.31QLAYY128 pKa = 9.55LTGFLAGLNTYY139 pKa = 10.45GLLEE143 pKa = 4.88KK144 pKa = 10.12YY145 pKa = 9.94CKK147 pKa = 9.85WSTASLWAWGLAQKK161 pKa = 9.24EE162 pKa = 4.35LPPPPEE168 pKa = 4.18FMSHH172 pKa = 6.08HH173 pKa = 6.34LASPFRR179 pKa = 11.84YY180 pKa = 9.4LFSSRR185 pKa = 11.84RR186 pKa = 11.84WKK188 pKa = 10.54RR189 pKa = 11.84AFKK192 pKa = 10.42ISNKK196 pKa = 9.27PSRR199 pKa = 11.84LEE201 pKa = 3.96QIFLAAFFKK210 pKa = 10.83DD211 pKa = 3.86LYY213 pKa = 8.84NCKK216 pKa = 9.92GAAAAVGPEE225 pKa = 4.6FIEE228 pKa = 4.17QALVKK233 pKa = 10.19HH234 pKa = 5.99KK235 pKa = 10.66EE236 pKa = 3.75ILTTPKK242 pKa = 10.1TPPTPVGVEE251 pKa = 3.91DD252 pKa = 4.04RR253 pKa = 11.84GKK255 pKa = 10.61DD256 pKa = 3.62LILEE260 pKa = 5.44AITQAAEE267 pKa = 4.32TIFGSVPRR275 pKa = 11.84KK276 pKa = 9.44NKK278 pKa = 9.35RR279 pKa = 11.84DD280 pKa = 3.35KK281 pKa = 10.72RR282 pKa = 11.84PPSRR286 pKa = 11.84FPSLNSSYY294 pKa = 11.04NYY296 pKa = 10.75SRR298 pKa = 11.84MKK300 pKa = 10.6GGAGGEE306 pKa = 3.9ILSQIDD312 pKa = 3.38EE313 pKa = 4.81ANDD316 pKa = 3.05EE317 pKa = 4.8FYY319 pKa = 11.28ALQPAEE325 pKa = 4.49GYY327 pKa = 8.61FWGYY331 pKa = 9.23AHH333 pKa = 7.21HH334 pKa = 6.56NAEE337 pKa = 4.85CIEE340 pKa = 3.82VRR342 pKa = 11.84TPYY345 pKa = 10.88DD346 pKa = 3.18PAIWQEE352 pKa = 3.97AEE354 pKa = 3.79ILSEE358 pKa = 3.94SRR360 pKa = 11.84AVAKK364 pKa = 10.31AAAGLGVDD372 pKa = 3.82AQVIPLSEE380 pKa = 4.06PFKK383 pKa = 11.15VRR385 pKa = 11.84TITKK389 pKa = 9.26GDD391 pKa = 3.55CDD393 pKa = 3.89MYY395 pKa = 10.99HH396 pKa = 7.59LARR399 pKa = 11.84RR400 pKa = 11.84WQKK403 pKa = 10.51VIHH406 pKa = 6.03SKK408 pKa = 8.23MRR410 pKa = 11.84KK411 pKa = 8.46QKK413 pKa = 9.34NCALIGRR420 pKa = 11.84PCDD423 pKa = 3.54GVFLSQIFYY432 pKa = 10.2EE433 pKa = 4.66SSHH436 pKa = 6.7FDD438 pKa = 3.09HH439 pKa = 7.07KK440 pKa = 11.32NEE442 pKa = 3.94KK443 pKa = 10.25KK444 pKa = 10.75GFFVSGDD451 pKa = 3.67YY452 pKa = 11.0EE453 pKa = 4.48SATDD457 pKa = 4.16LLNPEE462 pKa = 4.39LSLWAQNEE470 pKa = 3.91ISSRR474 pKa = 11.84LSIPLEE480 pKa = 3.87HH481 pKa = 6.43QRR483 pKa = 11.84VLNACLTEE491 pKa = 4.0HH492 pKa = 6.34NLYY495 pKa = 10.81YY496 pKa = 10.75GEE498 pKa = 4.89KK499 pKa = 10.04DD500 pKa = 3.42VNGNKK505 pKa = 8.18ITHH508 pKa = 5.75KK509 pKa = 8.87QTWGQLMGSPVSFPVLCLINLAATKK534 pKa = 9.97LAYY537 pKa = 9.67EE538 pKa = 4.09IYY540 pKa = 10.28YY541 pKa = 10.26RR542 pKa = 11.84EE543 pKa = 3.8EE544 pKa = 3.91LARR547 pKa = 11.84KK548 pKa = 7.67TGIDD552 pKa = 2.9INLIKK557 pKa = 10.72KK558 pKa = 9.87VDD560 pKa = 4.13LPIHH564 pKa = 5.71TLPMCVNGDD573 pKa = 5.05DD574 pKa = 3.73ILFWCHH580 pKa = 6.69SDD582 pKa = 2.72RR583 pKa = 11.84HH584 pKa = 4.29YY585 pKa = 10.96QIWKK589 pKa = 9.6EE590 pKa = 3.96VTANAGLKK598 pKa = 10.37FSLGKK603 pKa = 10.42NYY605 pKa = 7.33THH607 pKa = 7.92RR608 pKa = 11.84SYY610 pKa = 11.25CIINSEE616 pKa = 4.3MYY618 pKa = 9.78RR619 pKa = 11.84YY620 pKa = 9.06HH621 pKa = 7.76HH622 pKa = 6.52NEE624 pKa = 3.51SLPFVKK630 pKa = 10.31VPCLNTRR637 pKa = 11.84LIEE640 pKa = 4.4GGNRR644 pKa = 11.84STVTSVHH651 pKa = 6.71PLHH654 pKa = 6.84EE655 pKa = 5.52FDD657 pKa = 6.22FEE659 pKa = 4.43WLVKK663 pKa = 10.55DD664 pKa = 3.55NHH666 pKa = 6.33FNPKK670 pKa = 9.25KK671 pKa = 9.52HH672 pKa = 6.53LKK674 pKa = 10.07RR675 pKa = 11.84FCSKK679 pKa = 10.49VNPNHH684 pKa = 6.48TPLKK688 pKa = 10.12NLQLALKK695 pKa = 8.71EE696 pKa = 4.22RR697 pKa = 11.84KK698 pKa = 9.41AGPILYY704 pKa = 9.9NEE706 pKa = 4.15YY707 pKa = 10.92ASWFLTIEE715 pKa = 3.86ARR717 pKa = 11.84RR718 pKa = 11.84SVLQRR723 pKa = 11.84QATEE727 pKa = 3.97MLSDD731 pKa = 3.47KK732 pKa = 10.22PARR735 pKa = 11.84RR736 pKa = 11.84PAQIYY741 pKa = 9.97RR742 pKa = 11.84GQLMEE747 pKa = 5.78KK748 pKa = 9.96ILDD751 pKa = 3.53QRR753 pKa = 11.84FTKK756 pKa = 10.34VQFRR760 pKa = 11.84RR761 pKa = 11.84LNEE764 pKa = 3.61FRR766 pKa = 11.84ASLGSSLGKK775 pKa = 10.04SFPYY779 pKa = 10.3NLPQSLGGFGFPRR792 pKa = 11.84GPEE795 pKa = 3.66HH796 pKa = 7.42KK797 pKa = 9.91YY798 pKa = 8.92TVTDD802 pKa = 3.41YY803 pKa = 9.73RR804 pKa = 11.84TAALAYY810 pKa = 10.03SEE812 pKa = 4.21PKK814 pKa = 10.19EE815 pKa = 3.82FRR817 pKa = 11.84KK818 pKa = 9.73ALKK821 pKa = 9.67MVTPKK826 pKa = 10.52LARR829 pKa = 11.84AGFMSAVSKK838 pKa = 10.53EE839 pKa = 3.89LSFLSKK845 pKa = 10.69ALAIPEE851 pKa = 4.06VTQIVQYY858 pKa = 10.79EE859 pKa = 4.47SYY861 pKa = 10.9KK862 pKa = 10.79PVDD865 pKa = 3.39NGSIFEE871 pKa = 4.67LDD873 pKa = 3.77CLSGFVSPTNLVVGEE888 pKa = 4.72HH889 pKa = 6.15EE890 pKa = 4.12LSEE893 pKa = 4.25AFKK896 pKa = 11.1SRR898 pKa = 11.84VYY900 pKa = 9.11HH901 pKa = 4.93TRR903 pKa = 11.84EE904 pKa = 3.26ITRR907 pKa = 11.84ATKK910 pKa = 10.23YY911 pKa = 10.88LNRR914 pKa = 11.84KK915 pKa = 9.19YY916 pKa = 10.29NLQMRR921 pKa = 11.84GAEE924 pKa = 3.87KK925 pKa = 10.36RR926 pKa = 11.84RR927 pKa = 11.84AEE929 pKa = 4.08KK930 pKa = 10.51QLATDD935 pKa = 4.07LEE937 pKa = 4.42EE938 pKa = 6.0GIEE941 pKa = 4.14RR942 pKa = 11.84IEE944 pKa = 4.49TILLDD949 pKa = 4.07AFAEE953 pKa = 4.67SYY955 pKa = 10.2HH956 pKa = 6.58IQVVRR961 pKa = 11.84PSPRR965 pKa = 11.84FF966 pKa = 3.29

Molecular weight: 110.61 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KIM0|A0A1L3KIM0_9VIRU RNA-dependent RNA polymerase OS=Shahe narna-like virus 1 OX=1923429 PE=4 SV=1

MM1 pKa = 7.79EE2 pKa = 4.51SQQRR6 pKa = 11.84SLDD9 pKa = 3.74VVCQNLGQLRR19 pKa = 11.84KK20 pKa = 9.63RR21 pKa = 11.84CTGEE25 pKa = 4.63DD26 pKa = 2.99IITFVDD32 pKa = 3.63CWYY35 pKa = 8.87STTVHH40 pKa = 6.02EE41 pKa = 5.52VRR43 pKa = 11.84SPWISKK49 pKa = 9.34LRR51 pKa = 11.84PIDD54 pKa = 3.37VFHH57 pKa = 7.1YY58 pKa = 7.38YY59 pKa = 10.31TLVKK63 pKa = 9.53VAKK66 pKa = 9.98IGPTLDD72 pKa = 3.22THH74 pKa = 6.32PLEE77 pKa = 4.99DD78 pKa = 3.06VYY80 pKa = 11.39KK81 pKa = 9.1AVKK84 pKa = 10.04IISQYY89 pKa = 10.19TEE91 pKa = 3.77MTHH94 pKa = 6.84SYY96 pKa = 10.97SKK98 pKa = 11.11LLDD101 pKa = 3.56ASGYY105 pKa = 8.67GEE107 pKa = 5.04KK108 pKa = 9.75IRR110 pKa = 11.84QEE112 pKa = 4.08FSDD115 pKa = 3.88TLLSAFDD122 pKa = 4.4KK123 pKa = 11.23DD124 pKa = 3.31QLAYY128 pKa = 9.55LTGFLAGLNTYY139 pKa = 10.45GLLEE143 pKa = 4.88KK144 pKa = 10.12YY145 pKa = 9.94CKK147 pKa = 9.85WSTASLWAWGLAQKK161 pKa = 9.24EE162 pKa = 4.35LPPPPEE168 pKa = 4.18FMSHH172 pKa = 6.08HH173 pKa = 6.34LASPFRR179 pKa = 11.84YY180 pKa = 9.4LFSSRR185 pKa = 11.84RR186 pKa = 11.84WKK188 pKa = 10.54RR189 pKa = 11.84AFKK192 pKa = 10.42ISNKK196 pKa = 9.27PSRR199 pKa = 11.84LEE201 pKa = 3.96QIFLAAFFKK210 pKa = 10.83DD211 pKa = 3.86LYY213 pKa = 8.84NCKK216 pKa = 9.92GAAAAVGPEE225 pKa = 4.6FIEE228 pKa = 4.17QALVKK233 pKa = 10.19HH234 pKa = 5.99KK235 pKa = 10.66EE236 pKa = 3.75ILTTPKK242 pKa = 10.1TPPTPVGVEE251 pKa = 3.91DD252 pKa = 4.04RR253 pKa = 11.84GKK255 pKa = 10.61DD256 pKa = 3.62LILEE260 pKa = 5.44AITQAAEE267 pKa = 4.32TIFGSVPRR275 pKa = 11.84KK276 pKa = 9.44NKK278 pKa = 9.35RR279 pKa = 11.84DD280 pKa = 3.35KK281 pKa = 10.72RR282 pKa = 11.84PPSRR286 pKa = 11.84FPSLNSSYY294 pKa = 11.04NYY296 pKa = 10.75SRR298 pKa = 11.84MKK300 pKa = 10.6GGAGGEE306 pKa = 3.9ILSQIDD312 pKa = 3.38EE313 pKa = 4.81ANDD316 pKa = 3.05EE317 pKa = 4.8FYY319 pKa = 11.28ALQPAEE325 pKa = 4.49GYY327 pKa = 8.61FWGYY331 pKa = 9.23AHH333 pKa = 7.21HH334 pKa = 6.56NAEE337 pKa = 4.85CIEE340 pKa = 3.82VRR342 pKa = 11.84TPYY345 pKa = 10.88DD346 pKa = 3.18PAIWQEE352 pKa = 3.97AEE354 pKa = 3.79ILSEE358 pKa = 3.94SRR360 pKa = 11.84AVAKK364 pKa = 10.31AAAGLGVDD372 pKa = 3.82AQVIPLSEE380 pKa = 4.06PFKK383 pKa = 11.15VRR385 pKa = 11.84TITKK389 pKa = 9.26GDD391 pKa = 3.55CDD393 pKa = 3.89MYY395 pKa = 10.99HH396 pKa = 7.59LARR399 pKa = 11.84RR400 pKa = 11.84WQKK403 pKa = 10.51VIHH406 pKa = 6.03SKK408 pKa = 8.23MRR410 pKa = 11.84KK411 pKa = 8.46QKK413 pKa = 9.34NCALIGRR420 pKa = 11.84PCDD423 pKa = 3.54GVFLSQIFYY432 pKa = 10.2EE433 pKa = 4.66SSHH436 pKa = 6.7FDD438 pKa = 3.09HH439 pKa = 7.07KK440 pKa = 11.32NEE442 pKa = 3.94KK443 pKa = 10.25KK444 pKa = 10.75GFFVSGDD451 pKa = 3.67YY452 pKa = 11.0EE453 pKa = 4.48SATDD457 pKa = 4.16LLNPEE462 pKa = 4.39LSLWAQNEE470 pKa = 3.91ISSRR474 pKa = 11.84LSIPLEE480 pKa = 3.87HH481 pKa = 6.43QRR483 pKa = 11.84VLNACLTEE491 pKa = 4.0HH492 pKa = 6.34NLYY495 pKa = 10.81YY496 pKa = 10.75GEE498 pKa = 4.89KK499 pKa = 10.04DD500 pKa = 3.42VNGNKK505 pKa = 8.18ITHH508 pKa = 5.75KK509 pKa = 8.87QTWGQLMGSPVSFPVLCLINLAATKK534 pKa = 9.97LAYY537 pKa = 9.67EE538 pKa = 4.09IYY540 pKa = 10.28YY541 pKa = 10.26RR542 pKa = 11.84EE543 pKa = 3.8EE544 pKa = 3.91LARR547 pKa = 11.84KK548 pKa = 7.67TGIDD552 pKa = 2.9INLIKK557 pKa = 10.72KK558 pKa = 9.87VDD560 pKa = 4.13LPIHH564 pKa = 5.71TLPMCVNGDD573 pKa = 5.05DD574 pKa = 3.73ILFWCHH580 pKa = 6.69SDD582 pKa = 2.72RR583 pKa = 11.84HH584 pKa = 4.29YY585 pKa = 10.96QIWKK589 pKa = 9.6EE590 pKa = 3.96VTANAGLKK598 pKa = 10.37FSLGKK603 pKa = 10.42NYY605 pKa = 7.33THH607 pKa = 7.92RR608 pKa = 11.84SYY610 pKa = 11.25CIINSEE616 pKa = 4.3MYY618 pKa = 9.78RR619 pKa = 11.84YY620 pKa = 9.06HH621 pKa = 7.76HH622 pKa = 6.52NEE624 pKa = 3.51SLPFVKK630 pKa = 10.31VPCLNTRR637 pKa = 11.84LIEE640 pKa = 4.4GGNRR644 pKa = 11.84STVTSVHH651 pKa = 6.71PLHH654 pKa = 6.84EE655 pKa = 5.52FDD657 pKa = 6.22FEE659 pKa = 4.43WLVKK663 pKa = 10.55DD664 pKa = 3.55NHH666 pKa = 6.33FNPKK670 pKa = 9.25KK671 pKa = 9.52HH672 pKa = 6.53LKK674 pKa = 10.07RR675 pKa = 11.84FCSKK679 pKa = 10.49VNPNHH684 pKa = 6.48TPLKK688 pKa = 10.12NLQLALKK695 pKa = 8.71EE696 pKa = 4.22RR697 pKa = 11.84KK698 pKa = 9.41AGPILYY704 pKa = 9.9NEE706 pKa = 4.15YY707 pKa = 10.92ASWFLTIEE715 pKa = 3.86ARR717 pKa = 11.84RR718 pKa = 11.84SVLQRR723 pKa = 11.84QATEE727 pKa = 3.97MLSDD731 pKa = 3.47KK732 pKa = 10.22PARR735 pKa = 11.84RR736 pKa = 11.84PAQIYY741 pKa = 9.97RR742 pKa = 11.84GQLMEE747 pKa = 5.78KK748 pKa = 9.96ILDD751 pKa = 3.53QRR753 pKa = 11.84FTKK756 pKa = 10.34VQFRR760 pKa = 11.84RR761 pKa = 11.84LNEE764 pKa = 3.61FRR766 pKa = 11.84ASLGSSLGKK775 pKa = 10.04SFPYY779 pKa = 10.3NLPQSLGGFGFPRR792 pKa = 11.84GPEE795 pKa = 3.66HH796 pKa = 7.42KK797 pKa = 9.91YY798 pKa = 8.92TVTDD802 pKa = 3.41YY803 pKa = 9.73RR804 pKa = 11.84TAALAYY810 pKa = 10.03SEE812 pKa = 4.21PKK814 pKa = 10.19EE815 pKa = 3.82FRR817 pKa = 11.84KK818 pKa = 9.73ALKK821 pKa = 9.67MVTPKK826 pKa = 10.52LARR829 pKa = 11.84AGFMSAVSKK838 pKa = 10.53EE839 pKa = 3.89LSFLSKK845 pKa = 10.69ALAIPEE851 pKa = 4.06VTQIVQYY858 pKa = 10.79EE859 pKa = 4.47SYY861 pKa = 10.9KK862 pKa = 10.79PVDD865 pKa = 3.39NGSIFEE871 pKa = 4.67LDD873 pKa = 3.77CLSGFVSPTNLVVGEE888 pKa = 4.72HH889 pKa = 6.15EE890 pKa = 4.12LSEE893 pKa = 4.25AFKK896 pKa = 11.1SRR898 pKa = 11.84VYY900 pKa = 9.11HH901 pKa = 4.93TRR903 pKa = 11.84EE904 pKa = 3.26ITRR907 pKa = 11.84ATKK910 pKa = 10.23YY911 pKa = 10.88LNRR914 pKa = 11.84KK915 pKa = 9.19YY916 pKa = 10.29NLQMRR921 pKa = 11.84GAEE924 pKa = 3.87KK925 pKa = 10.36RR926 pKa = 11.84RR927 pKa = 11.84AEE929 pKa = 4.08KK930 pKa = 10.51QLATDD935 pKa = 4.07LEE937 pKa = 4.42EE938 pKa = 6.0GIEE941 pKa = 4.14RR942 pKa = 11.84IEE944 pKa = 4.49TILLDD949 pKa = 4.07AFAEE953 pKa = 4.67SYY955 pKa = 10.2HH956 pKa = 6.58IQVVRR961 pKa = 11.84PSPRR965 pKa = 11.84FF966 pKa = 3.29

Molecular weight: 110.61 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	966	966	966	966.0	110.61

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.246 ± 0.0	1.76 ± 0.0	4.037 ± 0.0	7.039 ± 0.0	4.762 ± 0.0	5.28 ± 0.0	3.209 ± 0.0	5.28 ± 0.0	7.35 ± 0.0	9.938 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.449 ± 0.0	3.934 ± 0.0	5.28 ± 0.0	3.727 ± 0.0	6.108 ± 0.0	7.246 ± 0.0	5.072 ± 0.0	5.176 ± 0.0	1.553 ± 0.0	4.555 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski