Sripur virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sripuvirus; Sripur sripuvirus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R1M1|A0A0D3R1M1_9RHAB Uncharacterized protein OS=Sripur virus OX=1620897 PE=4 SV=1
MM1 pKa = 7.49SFNPFTFPSSFEE13 pKa = 4.02NLSLKK18 pKa = 10.62AAIKK22 pKa = 10.58ASDD25 pKa = 4.0EE26 pKa = 4.03IDD28 pKa = 3.56EE29 pKa = 4.65EE30 pKa = 4.24EE31 pKa = 4.65ALVNDD36 pKa = 3.62AVEE39 pKa = 4.44KK40 pKa = 10.74KK41 pKa = 10.03KK42 pKa = 11.0EE43 pKa = 3.63SDD45 pKa = 2.61IFYY48 pKa = 10.35RR49 pKa = 11.84EE50 pKa = 4.16SNEE53 pKa = 4.08DD54 pKa = 3.27VQQIEE59 pKa = 4.11NWSFEE64 pKa = 4.41SEE66 pKa = 4.3GEE68 pKa = 4.41KK69 pKa = 10.52EE70 pKa = 5.06DD71 pKa = 3.56EE72 pKa = 4.36TQEE75 pKa = 4.21SEE77 pKa = 4.16EE78 pKa = 4.69SEE80 pKa = 4.56DD81 pKa = 4.47EE82 pKa = 4.33SGTDD86 pKa = 3.15EE87 pKa = 6.16GGYY90 pKa = 9.87DD91 pKa = 2.97WKK93 pKa = 11.09LPKK96 pKa = 9.15FTRR99 pKa = 11.84AEE101 pKa = 4.03LHH103 pKa = 5.63KK104 pKa = 10.3HH105 pKa = 5.16PEE107 pKa = 4.4KK108 pKa = 10.56ILCKK112 pKa = 9.5TVQALLFEE120 pKa = 4.42MGFGKK125 pKa = 10.66VKK127 pKa = 9.89MVLKK131 pKa = 10.39EE132 pKa = 4.03EE133 pKa = 4.52EE134 pKa = 4.59GLCSLFQEE142 pKa = 5.09DD143 pKa = 4.69DD144 pKa = 4.53SEE146 pKa = 6.98DD147 pKa = 3.93EE148 pKa = 4.63DD149 pKa = 5.55QDD151 pKa = 4.0DD152 pKa = 3.74QTSEE156 pKa = 4.18QEE158 pKa = 4.1DD159 pKa = 4.2EE160 pKa = 4.82EE161 pKa = 5.07ASSMYY166 pKa = 9.14PTDD169 pKa = 3.32KK170 pKa = 11.14AEE172 pKa = 4.18RR173 pKa = 11.84GTYY176 pKa = 8.77EE177 pKa = 3.85ATAPPAEE184 pKa = 4.26HH185 pKa = 6.01QEE187 pKa = 3.98EE188 pKa = 4.38RR189 pKa = 11.84EE190 pKa = 4.08EE191 pKa = 4.12AFSYY195 pKa = 10.27QNPGSSAHH203 pKa = 6.25VDD205 pKa = 3.35DD206 pKa = 7.11DD207 pKa = 4.35IMKK210 pKa = 10.31FLLKK214 pKa = 10.55LEE216 pKa = 4.55EE217 pKa = 4.26GVIVQDD223 pKa = 3.94LDD225 pKa = 3.61GDD227 pKa = 4.34HH228 pKa = 7.15VILNNDD234 pKa = 5.1LIGLTYY240 pKa = 11.16NEE242 pKa = 5.15MKK244 pKa = 10.68NIMITDD250 pKa = 4.19CTNIMLTLKK259 pKa = 10.23EE260 pKa = 4.32SVVQIPRR267 pKa = 11.84IQQTLAMIDD276 pKa = 3.63FDD278 pKa = 4.54SAA280 pKa = 3.4

Molecular weight: 31.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R288|A0A0D3R288_9RHAB Glycoprotein OS=Sripur virus OX=1620897 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.7LMRR5 pKa = 11.84KK6 pKa = 8.85KK7 pKa = 9.85HH8 pKa = 5.72LSMMQLKK15 pKa = 10.14RR16 pKa = 11.84KK17 pKa = 9.88RR18 pKa = 11.84NLIYY22 pKa = 9.52STEE25 pKa = 3.78RR26 pKa = 11.84VMRR29 pKa = 11.84MSNKK33 pKa = 9.61LRR35 pKa = 11.84TGVLNLRR42 pKa = 11.84GRR44 pKa = 11.84KK45 pKa = 8.95KK46 pKa = 10.08MKK48 pKa = 10.22LKK50 pKa = 9.27NQKK53 pKa = 8.72RR54 pKa = 11.84VKK56 pKa = 9.44MNQVQMKK63 pKa = 9.35GDD65 pKa = 4.06MIGSCQNSLEE75 pKa = 4.23QNCTNIRR82 pKa = 11.84KK83 pKa = 9.57KK84 pKa = 11.04SSVKK88 pKa = 10.32LSKK91 pKa = 10.34HH92 pKa = 6.06CCLKK96 pKa = 10.34WVLEE100 pKa = 4.02RR101 pKa = 11.84SKK103 pKa = 10.44WSSKK107 pKa = 9.14RR108 pKa = 11.84KK109 pKa = 9.4KK110 pKa = 10.53DD111 pKa = 3.85SAVCSKK117 pKa = 11.03KK118 pKa = 8.16MTVKK122 pKa = 9.84MRR124 pKa = 11.84IRR126 pKa = 11.84MIKK129 pKa = 10.14HH130 pKa = 6.07PSKK133 pKa = 10.93KK134 pKa = 9.07MKK136 pKa = 10.18KK137 pKa = 8.91QAACILLTKK146 pKa = 10.24PKK148 pKa = 9.31EE149 pKa = 4.02EE150 pKa = 4.06HH151 pKa = 6.33MKK153 pKa = 10.14PLHH156 pKa = 6.0LQLSIRR162 pKa = 11.84KK163 pKa = 8.9KK164 pKa = 8.29EE165 pKa = 4.11RR166 pKa = 11.84KK167 pKa = 9.33HH168 pKa = 7.12SVIKK172 pKa = 10.69IQDD175 pKa = 3.66HH176 pKa = 5.22QLMLMMII183 pKa = 4.79

Molecular weight: 21.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3935

66

2103

437.2

50.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.558 ± 0.707

2.618 ± 0.33

5.794 ± 0.544

6.404 ± 0.83

4.676 ± 0.446

5.032 ± 0.574

1.931 ± 0.247

8.208 ± 1.148

7.929 ± 1.181

9.835 ± 0.908

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.973 ± 0.738

5.413 ± 0.554

3.685 ± 0.457

3.609 ± 0.374

4.676 ± 0.47

7.929 ± 0.483

5.108 ± 0.353

5.21 ± 0.562

1.626 ± 0.22

3.787 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski