Wenzhou crab virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Chuviridae; Chuvivirus; Chuvivirus canceris

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KT97|A0A0B5KT97_9VIRU Putative nucleoprotein OS=Wenzhou crab virus 2 OX=1608092 GN=N PE=4 SV=1
MM1 pKa = 7.35VFFFLLVLCFARR13 pKa = 11.84GGLSAGPDD21 pKa = 3.62DD22 pKa = 4.09QVEE25 pKa = 3.99ALEE28 pKa = 5.59AYY30 pKa = 9.11DD31 pKa = 4.15CSGEE35 pKa = 4.1VSDD38 pKa = 5.72VSTISLNEE46 pKa = 3.77VKK48 pKa = 10.47EE49 pKa = 4.16CDD51 pKa = 3.52EE52 pKa = 4.34EE53 pKa = 4.27PTYY56 pKa = 11.1LEE58 pKa = 4.16SNQTIEE64 pKa = 4.17VQLLQDD70 pKa = 3.58SPNIVVDD77 pKa = 3.77VSTCRR82 pKa = 11.84LSISRR87 pKa = 11.84MIHH90 pKa = 5.09HH91 pKa = 7.5CGMHH95 pKa = 5.29SHH97 pKa = 6.7SSTVQAGMATYY108 pKa = 10.15VVEE111 pKa = 4.91LGRR114 pKa = 11.84SACLDD119 pKa = 3.23AVEE122 pKa = 4.35TRR124 pKa = 11.84RR125 pKa = 11.84VRR127 pKa = 11.84WSGTILDD134 pKa = 4.24GLSLGKK140 pKa = 8.39EE141 pKa = 4.04THH143 pKa = 6.04SLQTIAGSVTSGGKK157 pKa = 9.9CEE159 pKa = 3.93GSSYY163 pKa = 11.21SDD165 pKa = 3.9QFGSWSGVVVQAEE178 pKa = 3.74IKK180 pKa = 10.1MFIDD184 pKa = 4.87RR185 pKa = 11.84YY186 pKa = 9.42STTANIRR193 pKa = 11.84TGDD196 pKa = 3.17ITIRR200 pKa = 11.84GGLRR204 pKa = 11.84CEE206 pKa = 4.57WDD208 pKa = 3.51SQSCFDD214 pKa = 3.76NMAGEE219 pKa = 4.54TYY221 pKa = 8.95WRR223 pKa = 11.84KK224 pKa = 10.26KK225 pKa = 10.18EE226 pKa = 5.23DD227 pKa = 3.49GACPEE232 pKa = 4.0KK233 pKa = 11.12SFDD236 pKa = 4.01VLYY239 pKa = 10.34QGPAVRR245 pKa = 11.84VSSLRR250 pKa = 11.84LGTTHH255 pKa = 6.83HH256 pKa = 6.91MYY258 pKa = 10.35HH259 pKa = 7.06ISAGDD264 pKa = 3.59FQFVTQSGHH273 pKa = 5.52QDD275 pKa = 3.79YY276 pKa = 10.73LCHH279 pKa = 5.62QAVYY283 pKa = 8.66RR284 pKa = 11.84TDD286 pKa = 3.15HH287 pKa = 6.31PRR289 pKa = 11.84LYY291 pKa = 9.94IQEE294 pKa = 4.07RR295 pKa = 11.84PAPGFYY301 pKa = 10.12FKK303 pKa = 10.75KK304 pKa = 10.47KK305 pKa = 9.89SLQVRR310 pKa = 11.84NTDD313 pKa = 3.19LMQYY317 pKa = 9.69TNAKK321 pKa = 8.41LTLLEE326 pKa = 4.17HH327 pKa = 6.67HH328 pKa = 6.91FEE330 pKa = 4.21EE331 pKa = 5.34QLGQLYY337 pKa = 10.23QKK339 pKa = 10.76LRR341 pKa = 11.84LDD343 pKa = 3.22TCLAQRR349 pKa = 11.84TALQNKK355 pKa = 9.73LSLLKK360 pKa = 10.87LKK362 pKa = 10.42GSHH365 pKa = 6.47GGQHH369 pKa = 5.88SLGGRR374 pKa = 11.84GTKK377 pKa = 9.85TIVAGEE383 pKa = 4.09VAHH386 pKa = 6.73VIRR389 pKa = 11.84CRR391 pKa = 11.84RR392 pKa = 11.84VRR394 pKa = 11.84VTTRR398 pKa = 11.84PSSEE402 pKa = 4.63CYY404 pKa = 10.33AEE406 pKa = 4.96LPVTYY411 pKa = 9.98GEE413 pKa = 4.4KK414 pKa = 10.38AGCILPISRR423 pKa = 11.84IIVPHH428 pKa = 5.94CTPTPCSDD436 pKa = 5.39IIPQKK441 pKa = 9.8WKK443 pKa = 10.01IGRR446 pKa = 11.84RR447 pKa = 11.84WISLDD452 pKa = 3.04PKK454 pKa = 10.49PRR456 pKa = 11.84VTLPPVVLDD465 pKa = 3.87PAGKK469 pKa = 8.14TKK471 pKa = 10.18WEE473 pKa = 4.0YY474 pKa = 10.97KK475 pKa = 9.5PLRR478 pKa = 11.84EE479 pKa = 4.16LSIAGIYY486 pKa = 7.8STSQLEE492 pKa = 4.04QTQDD496 pKa = 3.33VIMNSHH502 pKa = 4.73TRR504 pKa = 11.84EE505 pKa = 4.07VVSSIISGRR514 pKa = 11.84VTGHH518 pKa = 7.25DD519 pKa = 3.92PDD521 pKa = 4.15DD522 pKa = 3.82QKK524 pKa = 11.83LAMSRR529 pKa = 11.84VIPMEE534 pKa = 3.66QLRR537 pKa = 11.84ASMSEE542 pKa = 3.8AFGTLTAWVLEE553 pKa = 4.36AGSAFGAVMLAYY565 pKa = 8.6YY566 pKa = 8.94TYY568 pKa = 10.65QAGKK572 pKa = 8.12TILSAVLNFKK582 pKa = 9.36MMRR585 pKa = 11.84EE586 pKa = 4.09MKK588 pKa = 10.44GCSAWLFTAPCSVVTLWLQHH608 pKa = 6.32RR609 pKa = 11.84SRR611 pKa = 11.84SSAGKK616 pKa = 9.65EE617 pKa = 3.89EE618 pKa = 4.43SCIGMVSEE626 pKa = 5.35AGPEE630 pKa = 4.06EE631 pKa = 4.3SRR633 pKa = 11.84LVHH636 pKa = 6.65PATIPGAVKK645 pKa = 10.05VYY647 pKa = 10.68PNVPEE652 pKa = 3.81QQ653 pKa = 3.2

Molecular weight: 72.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KT97|A0A0B5KT97_9VIRU Putative nucleoprotein OS=Wenzhou crab virus 2 OX=1608092 GN=N PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 5.67KK3 pKa = 10.24LVPYY7 pKa = 7.88EE8 pKa = 3.8FPIRR12 pKa = 11.84RR13 pKa = 11.84GPHH16 pKa = 5.8PPYY19 pKa = 10.89GPMEE23 pKa = 4.35IEE25 pKa = 4.59NEE27 pKa = 4.07DD28 pKa = 3.63STTINPPAGLKK39 pKa = 10.3EE40 pKa = 4.25SIEE43 pKa = 4.25VFFLSYY49 pKa = 11.07QEE51 pKa = 4.43VLLTTTIPTTRR62 pKa = 11.84SGAKK66 pKa = 9.65KK67 pKa = 8.57GTRR70 pKa = 11.84KK71 pKa = 10.4GKK73 pKa = 8.58VTMSQQASSQTAGPQAPAAPSPAPVAQGTGNGTTPGNVIQGGSAPPALNAPGPQSLQAPPQTSASVHH140 pKa = 6.41PPTPVPSTAGQAPILAPASSAPYY163 pKa = 9.67QGPTFIPAGSSQLVQMTHH181 pKa = 6.44PVLPVHH187 pKa = 6.26IQGAGFNPMYY197 pKa = 10.28QGFYY201 pKa = 7.7NTPMATAAGPSQVAYY216 pKa = 9.46PGAGWPSQPQNNPGAQAPPAPAFTFNAGPASNKK249 pKa = 7.39VQWGVAFGRR258 pKa = 11.84DD259 pKa = 3.29LKK261 pKa = 11.34YY262 pKa = 11.04DD263 pKa = 3.73AFRR266 pKa = 11.84LKK268 pKa = 10.64NLNHH272 pKa = 6.6GVFQGAWDD280 pKa = 4.15DD281 pKa = 4.45ADD283 pKa = 3.9LLLYY287 pKa = 9.89VAFYY291 pKa = 11.03GRR293 pKa = 11.84VRR295 pKa = 11.84AQVVGTNMMYY305 pKa = 10.83AVTIFAIGEE314 pKa = 4.4GIMDD318 pKa = 5.35CLPDD322 pKa = 4.5SAYY325 pKa = 10.96LDD327 pKa = 3.57QAQAQAAFIQDD338 pKa = 3.21ISSLFPLSSVGWLRR352 pKa = 11.84VDD354 pKa = 2.84ATTYY358 pKa = 11.61ANALTNEE365 pKa = 3.93LRR367 pKa = 11.84GLNLPATHH375 pKa = 6.83SEE377 pKa = 4.13GLAQALDD384 pKa = 3.58LRR386 pKa = 11.84GAYY389 pKa = 10.04AVMVWLAKK397 pKa = 10.42RR398 pKa = 11.84LTASVRR404 pKa = 11.84KK405 pKa = 8.23TEE407 pKa = 4.45IILWTLVALVKK418 pKa = 10.5QGNISDD424 pKa = 3.93RR425 pKa = 11.84FTNKK429 pKa = 8.02ITQSLEE435 pKa = 3.74QEE437 pKa = 4.26VGVTVTLPSDD447 pKa = 4.65GIRR450 pKa = 11.84AVWDD454 pKa = 3.61VYY456 pKa = 11.13GPYY459 pKa = 9.94INEE462 pKa = 3.86VTAGPVFNRR471 pKa = 11.84WLPLVPQNALRR482 pKa = 11.84VRR484 pKa = 11.84LTILQAAGEE493 pKa = 4.33GLTAFTTILKK503 pKa = 10.22ALRR506 pKa = 11.84AHH508 pKa = 7.0PRR510 pKa = 11.84FHH512 pKa = 7.01WSRR515 pKa = 11.84VINLFPQEE523 pKa = 3.27WDD525 pKa = 3.27NFVNAVTAVGANTFYY540 pKa = 11.19GFKK543 pKa = 10.15KK544 pKa = 10.36DD545 pKa = 3.56LGVVRR550 pKa = 11.84STMYY554 pKa = 11.09KK555 pKa = 9.64NLGYY559 pKa = 9.76VAKK562 pKa = 9.8EE563 pKa = 3.7LMVKK567 pKa = 10.56VSGDD571 pKa = 3.35APLNFYY577 pKa = 10.88AGWTRR582 pKa = 11.84IPAFRR587 pKa = 11.84TIIDD591 pKa = 3.6QMVVDD596 pKa = 4.94YY597 pKa = 9.25EE598 pKa = 4.21AQRR601 pKa = 11.84AADD604 pKa = 3.79LTGGGHH610 pKa = 7.26AAYY613 pKa = 10.39LPTADD618 pKa = 4.77AALSTPAIMSSTFPLNN634 pKa = 3.07

Molecular weight: 67.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3495

634

2208

1165.0

130.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.754 ± 1.46

1.888 ± 0.541

4.578 ± 0.421

5.522 ± 0.763

3.72 ± 0.364

6.094 ± 1.114

2.918 ± 0.458

4.835 ± 0.171

4.578 ± 0.545

9.986 ± 0.884

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.804 ± 0.237

3.348 ± 0.556

5.608 ± 1.053

4.435 ± 0.506

5.608 ± 0.505

7.382 ± 0.682

6.552 ± 0.404

6.981 ± 0.331

1.574 ± 0.049

3.834 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski