Tortoise microvirus 86
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FW16|A0A4V1FW16_9VIRU Uncharacterized protein OS=Tortoise microvirus 86 OX=2583194 PE=4 SV=1
MM1 pKa = 7.12 QCACHH6 pKa = 5.19 GTAYY10 pKa = 11.15 LMAFNSTWQDD20 pKa = 2.22 IGYY23 pKa = 9.14 RR24 pKa = 11.84 GAMANSPQGEE34 pKa = 4.48 TCLGGSGGSEE44 pKa = 3.47 PHH46 pKa = 6.53 ALISLDD52 pKa = 3.36 ILVLIDD58 pKa = 3.44 TKK60 pKa = 10.08 YY61 pKa = 10.79 KK62 pKa = 10.52 CVAICC67 pKa = 4.53
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.417
IPC2_protein 5.359
IPC_protein 5.13
Toseland 5.245
ProMoST 5.397
Dawson 5.27
Bjellqvist 5.334
Wikipedia 5.245
Rodwell 5.219
Grimsley 5.245
Solomon 5.27
Lehninger 5.245
Nozaki 5.474
DTASelect 5.664
Thurlkill 5.486
EMBOSS 5.461
Sillero 5.55
Patrickios 0.172
IPC_peptide 5.283
IPC2_peptide 5.55
IPC2.peptide.svr19 5.551
Protein with the highest isoelectric point:
>tr|A0A4P8W7U1|A0A4P8W7U1_9VIRU Major capsid protein OS=Tortoise microvirus 86 OX=2583194 PE=3 SV=1
MM1 pKa = 7.5 RR2 pKa = 11.84 RR3 pKa = 11.84 SNNWQFRR10 pKa = 11.84 LLQQEE15 pKa = 4.22 KK16 pKa = 9.38 VAASASFVTLTYY28 pKa = 10.07 NTDD31 pKa = 3.59 TVPLSPRR38 pKa = 11.84 GYY40 pKa = 7.82 MTLCKK45 pKa = 9.71 RR46 pKa = 11.84 DD47 pKa = 3.36 FQLFMKK53 pKa = 10.57 RR54 pKa = 11.84 LRR56 pKa = 11.84 KK57 pKa = 9.52 LHH59 pKa = 6.15 AKK61 pKa = 8.83 NVKK64 pKa = 9.07 LRR66 pKa = 11.84 YY67 pKa = 8.75 YY68 pKa = 10.98 AVGEE72 pKa = 4.24 YY73 pKa = 10.57 GSKK76 pKa = 8.61 TFRR79 pKa = 11.84 PHH81 pKa = 4.72 FHH83 pKa = 7.16 AIIYY87 pKa = 10.44 DD88 pKa = 3.95 LDD90 pKa = 3.89 RR91 pKa = 11.84 EE92 pKa = 4.28 KK93 pKa = 10.94 LAEE96 pKa = 3.75 AWQNGDD102 pKa = 3.52 CHH104 pKa = 7.98 IGDD107 pKa = 3.51 VSGASIAYY115 pKa = 5.6 TVKK118 pKa = 10.53 YY119 pKa = 6.83 MHH121 pKa = 7.07 KK122 pKa = 10.21 GKK124 pKa = 10.14 IIPMHH129 pKa = 6.66 RR130 pKa = 11.84 NDD132 pKa = 3.78 DD133 pKa = 4.48 RR134 pKa = 11.84 IPEE137 pKa = 4.17 FSLMSKK143 pKa = 10.71 GLGKK147 pKa = 10.45 NYY149 pKa = 7.28 MTPQMVNYY157 pKa = 9.21 HH158 pKa = 6.38 KK159 pKa = 10.91 EE160 pKa = 4.36 DD161 pKa = 2.96 ISRR164 pKa = 11.84 NYY166 pKa = 8.09 VTLEE170 pKa = 4.04 GGVKK174 pKa = 9.82 VAMPRR179 pKa = 11.84 YY180 pKa = 8.37 YY181 pKa = 10.07 RR182 pKa = 11.84 EE183 pKa = 3.95 KK184 pKa = 9.97 IYY186 pKa = 10.69 TEE188 pKa = 4.05 EE189 pKa = 3.75 EE190 pKa = 3.82 RR191 pKa = 11.84 NRR193 pKa = 11.84 QNLINEE199 pKa = 4.38 KK200 pKa = 10.24 SATRR204 pKa = 11.84 ALDD207 pKa = 3.44 EE208 pKa = 5.35 RR209 pKa = 11.84 EE210 pKa = 3.33 TDD212 pKa = 3.7 YY213 pKa = 11.15 IGRR216 pKa = 11.84 TGTDD220 pKa = 3.04 DD221 pKa = 3.48 YY222 pKa = 11.7 LRR224 pKa = 11.84 ADD226 pKa = 4.28 FEE228 pKa = 4.89 SKK230 pKa = 9.96 KK231 pKa = 10.96 AKK233 pKa = 10.43 LEE235 pKa = 3.99 NFRR238 pKa = 11.84 KK239 pKa = 9.91 RR240 pKa = 11.84 AGDD243 pKa = 3.41 NRR245 pKa = 11.84 KK246 pKa = 8.18 TII248 pKa = 3.75
Molecular weight: 29.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.884
IPC2_protein 9.18
IPC_protein 9.151
Toseland 9.692
ProMoST 9.487
Dawson 9.97
Bjellqvist 9.677
Wikipedia 10.16
Rodwell 10.248
Grimsley 10.058
Solomon 9.999
Lehninger 9.94
Nozaki 9.706
DTASelect 9.663
Thurlkill 9.794
EMBOSS 10.116
Sillero 9.882
Patrickios 5.181
IPC_peptide 9.984
IPC2_peptide 8.273
IPC2.peptide.svr19 8.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1284
67
525
256.8
28.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.411 ± 0.947
0.623 ± 0.612
5.841 ± 0.448
5.763 ± 0.65
3.66 ± 0.774
6.542 ± 0.624
2.492 ± 0.451
5.14 ± 0.618
5.997 ± 0.72
8.022 ± 0.698
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.193 ± 0.246
5.218 ± 0.798
4.984 ± 0.913
5.218 ± 0.875
5.685 ± 0.793
6.854 ± 0.549
5.919 ± 0.189
4.595 ± 0.741
1.168 ± 0.148
4.673 ± 0.718
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here