Sulfuricella denitrificans (strain DSM 22764 / NBRC 105220 / skB26)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Sulfuricellaceae; Sulfuricella; Sulfuricella denitrificans

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6AF13|S6AF13_SULDS UDP-N-acetylglucosamine 1-carboxyvinyltransferase OS=Sulfuricella denitrificans (strain DSM 22764 / NBRC 105220 / skB26) OX=1163617 GN=murA PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.51NNPLFKK8 pKa = 9.5TCLVAALVISGSVSTAQAATITFDD32 pKa = 4.53NLDD35 pKa = 3.9TSSGSYY41 pKa = 10.37AQLQNGYY48 pKa = 11.09AGFNWDD54 pKa = 4.1NFWAVDD60 pKa = 3.42GLNYY64 pKa = 10.1YY65 pKa = 10.32DD66 pKa = 5.05PNDD69 pKa = 3.72PTAIGYY75 pKa = 5.22TTGVVSPNNVAFNGFADD92 pKa = 4.07PASFSSTTIFSLEE105 pKa = 3.83SAYY108 pKa = 8.69VTKK111 pKa = 10.81ARR113 pKa = 11.84DD114 pKa = 3.14AGLTHH119 pKa = 7.04FDD121 pKa = 3.98GYY123 pKa = 11.52NGASLLYY130 pKa = 11.13SMDD133 pKa = 3.46VYY135 pKa = 11.05STTTAPTFVTFNWAGLDD152 pKa = 3.6KK153 pKa = 11.2VVMSDD158 pKa = 3.73GDD160 pKa = 4.16GTAHH164 pKa = 6.58TAIDD168 pKa = 5.27DD169 pKa = 3.68ITVNSVPVPAAAWLLGSGLLGLIGVARR196 pKa = 11.84RR197 pKa = 11.84KK198 pKa = 9.46VAA200 pKa = 3.33

Molecular weight: 21.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6AMC0|S6AMC0_SULDS Type IV pilus assembly protein PilW OS=Sulfuricella denitrificans (strain DSM 22764 / NBRC 105220 / skB26) OX=1163617 GN=SCD_n02125 PE=4 SV=1
MM1 pKa = 7.25VSSLNRR7 pKa = 11.84GFVIFAVLCAASWTTAVFATSCRR30 pKa = 11.84EE31 pKa = 3.62AAHH34 pKa = 6.84ALNQRR39 pKa = 11.84LQPKK43 pKa = 9.49IDD45 pKa = 3.63EE46 pKa = 4.73AEE48 pKa = 4.13LANMLDD54 pKa = 3.57VLNEE58 pKa = 3.94SRR60 pKa = 11.84NTQLPGKK67 pKa = 9.79FVTKK71 pKa = 10.44RR72 pKa = 11.84QAQQSGWRR80 pKa = 11.84PGRR83 pKa = 11.84DD84 pKa = 3.19LWNVPALRR92 pKa = 11.84FKK94 pKa = 11.13SIGGDD99 pKa = 2.82HH100 pKa = 6.88FGNRR104 pKa = 11.84EE105 pKa = 3.44GRR107 pKa = 11.84LPRR110 pKa = 11.84GDD112 pKa = 3.12WRR114 pKa = 11.84EE115 pKa = 3.08ADD117 pKa = 3.22LNYY120 pKa = 10.61RR121 pKa = 11.84GGHH124 pKa = 5.94RR125 pKa = 11.84GAKK128 pKa = 9.2RR129 pKa = 11.84LLFSRR134 pKa = 11.84EE135 pKa = 3.28GLRR138 pKa = 11.84RR139 pKa = 11.84VTVDD143 pKa = 3.17HH144 pKa = 6.23YY145 pKa = 9.96QTFVEE150 pKa = 4.93VPPCRR155 pKa = 3.66

Molecular weight: 17.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3071

0

3071

966249

46

3317

314.6

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.374 ± 0.053

0.991 ± 0.021

5.225 ± 0.033

6.146 ± 0.052

3.81 ± 0.03

7.764 ± 0.047

2.386 ± 0.023

5.549 ± 0.03

4.517 ± 0.041

10.905 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.674 ± 0.024

3.308 ± 0.028

4.649 ± 0.03

3.901 ± 0.03

5.973 ± 0.043

5.696 ± 0.039

5.053 ± 0.036

7.176 ± 0.044

1.278 ± 0.017

2.625 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski