Moniliophthora roreri (strain MCA 2997) (Cocoa frosty pod rot fungus) (Crinipellis roreri)
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17976 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V2X2R2|V2X2R2_MONRO Nwd2 OS=Moniliophthora roreri (strain MCA 2997) OX=1381753 GN=Moror_15220 PE=4 SV=1
MM1 pKa = 6.98 NHH3 pKa = 6.72 HH4 pKa = 7.28 LEE6 pKa = 4.29 TMSPYY11 pKa = 10.47 SPCEE15 pKa = 3.36 EE16 pKa = 5.37 DD17 pKa = 4.29 EE18 pKa = 4.08 IQEE21 pKa = 4.16 ILSQFYY27 pKa = 9.13 LTDD30 pKa = 3.37 ATEE33 pKa = 4.81 SEE35 pKa = 4.42 DD36 pKa = 4.89 TDD38 pKa = 4.82 LCDD41 pKa = 5.91 LDD43 pKa = 4.82 IEE45 pKa = 4.62 SSLYY49 pKa = 10.58 RR50 pKa = 11.84 SDD52 pKa = 4.56 EE53 pKa = 4.04 EE54 pKa = 4.4 EE55 pKa = 4.27 EE56 pKa = 4.49 DD57 pKa = 3.74 VEE59 pKa = 5.39 LYY61 pKa = 10.49 EE62 pKa = 6.14 LYY64 pKa = 11.06 GDD66 pKa = 5.87 DD67 pKa = 6.37 DD68 pKa = 6.78 DD69 pKa = 7.76 DD70 pKa = 7.78 DD71 pKa = 7.48 DD72 pKa = 7.59 DD73 pKa = 7.63 DD74 pKa = 6.84 DD75 pKa = 6.9 DD76 pKa = 5.21 NDD78 pKa = 3.71 HH79 pKa = 7.3 DD80 pKa = 4.83 RR81 pKa = 11.84 YY82 pKa = 10.69 SYY84 pKa = 11.11 SSRR87 pKa = 11.84 EE88 pKa = 3.93 SDD90 pKa = 4.63 EE91 pKa = 5.15 SDD93 pKa = 3.74 LDD95 pKa = 3.98 YY96 pKa = 11.26 PPDD99 pKa = 4.65 PPTEE103 pKa = 4.06 NYY105 pKa = 10.32 LPSFQDD111 pKa = 3.05 SCQDD115 pKa = 3.06 GEE117 pKa = 4.57 LRR119 pKa = 11.84 SDD121 pKa = 4.81 PIDD124 pKa = 3.78 SFHH127 pKa = 7.7 DD128 pKa = 3.25 ATYY131 pKa = 10.68 RR132 pKa = 11.84 NSKK135 pKa = 9.5 MSDD138 pKa = 2.83 VSLTIDD144 pKa = 3.26 GLGSRR149 pKa = 11.84 EE150 pKa = 3.89 EE151 pKa = 4.15 PSRR154 pKa = 11.84 GRR156 pKa = 11.84 PLQKK160 pKa = 10.99 KK161 pKa = 8.78 MIGWWASASPGHH173 pKa = 6.23 EE174 pKa = 4.46 LYY176 pKa = 10.64 QQVASRR182 pKa = 11.84 HH183 pKa = 5.52 DD184 pKa = 3.83 SEE186 pKa = 4.73 SS187 pKa = 3.11
Molecular weight: 21.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.719
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.427
Solomon 3.732
Lehninger 3.694
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.872
Patrickios 1.252
IPC_peptide 3.732
IPC2_peptide 3.846
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|V2YDX6|V2YDX6_MONRO Uncharacterized protein (Fragment) OS=Moniliophthora roreri (strain MCA 2997) OX=1381753 GN=Moror_792 PE=4 SV=1
QQ1 pKa = 6.53 GQNNGQQNNGQQGAQPGAAPAGANNGANGGTAGQQGAQQGQQGAQPGAAPAQQGQNRR58 pKa = 11.84 GGNRR62 pKa = 11.84 GGRR65 pKa = 11.84 GRR67 pKa = 11.84 GRR69 pKa = 11.84 QQRR72 pKa = 11.84 SLLSRR77 pKa = 11.84 MSLL80 pKa = 3.35
Molecular weight: 7.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17972
4
17976
7359806
23
5026
409.4
45.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.637 ± 0.015
1.267 ± 0.008
5.541 ± 0.013
6.033 ± 0.017
3.948 ± 0.013
6.326 ± 0.019
2.507 ± 0.008
5.278 ± 0.016
4.882 ± 0.017
9.302 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.008
3.799 ± 0.011
6.277 ± 0.022
3.801 ± 0.011
5.828 ± 0.015
8.753 ± 0.03
6.114 ± 0.012
6.221 ± 0.014
1.515 ± 0.007
2.824 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here