Cumuto virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Goukovirus; Cumuto goukovirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5VH60|W5VH60_9VIRU Glycoprotein G OS=Cumuto virus OX=1457166 PE=4 SV=1
MM1 pKa = 7.68ASVKK5 pKa = 10.42LLFVIYY11 pKa = 9.81CISSLMINEE20 pKa = 3.94MRR22 pKa = 11.84TVEE25 pKa = 5.16LPEE28 pKa = 3.97FWHH31 pKa = 6.3MSRR34 pKa = 11.84EE35 pKa = 3.94KK36 pKa = 10.35MNRR39 pKa = 11.84KK40 pKa = 9.26PIDD43 pKa = 3.64PNVKK47 pKa = 10.01VKK49 pKa = 10.79GGNCSNGVPANATIQDD65 pKa = 3.61EE66 pKa = 5.17GITLNKK72 pKa = 9.82CSKK75 pKa = 10.75QMTAVVRR82 pKa = 11.84YY83 pKa = 6.61TVEE86 pKa = 4.23GVDD89 pKa = 3.36EE90 pKa = 4.41TFGCVVNYY98 pKa = 10.61DD99 pKa = 3.88CGLNLHH105 pKa = 6.63NGDD108 pKa = 4.82GYY110 pKa = 10.23CTLKK114 pKa = 11.15NEE116 pKa = 4.62DD117 pKa = 4.34CFHH120 pKa = 6.88EE121 pKa = 4.81PGCIVPKK128 pKa = 10.7DD129 pKa = 4.06RR130 pKa = 11.84ILPMGNCNEE139 pKa = 4.34THH141 pKa = 5.39WTQNRR146 pKa = 11.84PEE148 pKa = 4.06FCSVGVHH155 pKa = 6.38KK156 pKa = 10.72AGCEE160 pKa = 3.62EE161 pKa = 3.97TGRR164 pKa = 11.84VVRR167 pKa = 11.84LPWVVDD173 pKa = 3.72RR174 pKa = 11.84SGKK177 pKa = 9.74KK178 pKa = 9.96RR179 pKa = 11.84LINKK183 pKa = 9.23LNIEE187 pKa = 4.39MAQTMSDD194 pKa = 3.73GEE196 pKa = 4.75SYY198 pKa = 9.99WEE200 pKa = 4.14DD201 pKa = 2.92KK202 pKa = 10.56RR203 pKa = 11.84YY204 pKa = 8.87EE205 pKa = 4.22TMGPVQGDD213 pKa = 3.38NNYY216 pKa = 9.96CDD218 pKa = 3.5QHH220 pKa = 8.16NGDD223 pKa = 3.75VDD225 pKa = 4.33CNKK228 pKa = 10.2HH229 pKa = 5.8LGSDD233 pKa = 4.0GEE235 pKa = 4.36HH236 pKa = 6.2TFRR239 pKa = 11.84VLKK242 pKa = 10.72DD243 pKa = 3.52EE244 pKa = 4.81SNTPVLHH251 pKa = 7.15LGTCKK256 pKa = 10.1TPVYY260 pKa = 10.46GFGYY264 pKa = 9.64EE265 pKa = 3.97DD266 pKa = 3.75VIVFSEE272 pKa = 4.18IEE274 pKa = 3.72KK275 pKa = 9.22DD276 pKa = 3.55TRR278 pKa = 11.84LSCPNCHH285 pKa = 6.2TSCDD289 pKa = 4.02SEE291 pKa = 4.81SMSIVVPEE299 pKa = 3.89ATKK302 pKa = 10.93KK303 pKa = 10.18NVRR306 pKa = 11.84ICGRR310 pKa = 11.84QNCVLIQSSDD320 pKa = 3.62KK321 pKa = 10.5IIKK324 pKa = 7.65VQRR327 pKa = 11.84DD328 pKa = 3.73FHH330 pKa = 7.66QKK332 pKa = 8.21ITDD335 pKa = 3.27EE336 pKa = 4.05RR337 pKa = 11.84VIFEE341 pKa = 4.2VSDD344 pKa = 3.67DD345 pKa = 3.88EE346 pKa = 4.44KK347 pKa = 10.39TYY349 pKa = 10.8SYY351 pKa = 11.32SLTQSCPVVEE361 pKa = 4.0ICEE364 pKa = 5.25AIDD367 pKa = 3.35CHH369 pKa = 7.66YY370 pKa = 11.49CMIRR374 pKa = 11.84LYY376 pKa = 11.14NPTCYY381 pKa = 10.08EE382 pKa = 3.61WFHH385 pKa = 6.17WVMVAGILYY394 pKa = 8.7TLVIFFGLLLLMLKK408 pKa = 10.15PIIKK412 pKa = 10.05LVWFLICIIYY422 pKa = 9.71KK423 pKa = 10.12IIMKK427 pKa = 10.33LSGIMIGKK435 pKa = 8.62SKK437 pKa = 10.7KK438 pKa = 10.44GFSAVNKK445 pKa = 9.85FADD448 pKa = 4.11EE449 pKa = 4.11EE450 pKa = 4.97SSMEE454 pKa = 3.87NLIYY458 pKa = 10.64KK459 pKa = 7.18PTSIVKK465 pKa = 9.89KK466 pKa = 8.87NKK468 pKa = 9.02PMKK471 pKa = 10.16PMIFIILILGLIVTICEE488 pKa = 4.25SKK490 pKa = 10.39LCSNVVIDD498 pKa = 4.93TIPSQVCTKK507 pKa = 10.44IGDD510 pKa = 3.58QYY512 pKa = 10.06RR513 pKa = 11.84CKK515 pKa = 9.91IGSVVEE521 pKa = 4.18VPMISYY527 pKa = 9.96DD528 pKa = 4.19QISCLHH534 pKa = 6.73FISPKK539 pKa = 10.29GNTIGEE545 pKa = 4.17LQITPMDD552 pKa = 4.49LGFKK556 pKa = 9.86CLKK559 pKa = 9.41EE560 pKa = 3.87NLYY563 pKa = 8.59FTRR566 pKa = 11.84DD567 pKa = 3.35VKK569 pKa = 10.79FHH571 pKa = 6.33VDD573 pKa = 2.92HH574 pKa = 7.54MIRR577 pKa = 11.84CPHH580 pKa = 6.8AGEE583 pKa = 5.96CSDD586 pKa = 4.5DD587 pKa = 3.19WCKK590 pKa = 9.69TVVRR594 pKa = 11.84EE595 pKa = 4.24TKK597 pKa = 10.53VEE599 pKa = 3.98GLSEE603 pKa = 4.24LKK605 pKa = 10.81GMYY608 pKa = 9.66DD609 pKa = 3.28QSCKK613 pKa = 10.54LGSACAGAGCFFCTNSCHH631 pKa = 4.7TTRR634 pKa = 11.84YY635 pKa = 9.77RR636 pKa = 11.84PVPVSGSVYY645 pKa = 10.34EE646 pKa = 4.85IFYY649 pKa = 9.59CPKK652 pKa = 9.01WEE654 pKa = 4.15PSGTFHH660 pKa = 7.59FEE662 pKa = 3.91WKK664 pKa = 10.34GSASTVSTTLNLIHH678 pKa = 6.54GQTVTISADD687 pKa = 2.87IRR689 pKa = 11.84ATLQMTVQAKK699 pKa = 10.52LPVLGKK705 pKa = 10.8KK706 pKa = 10.46FMQKK710 pKa = 10.38DD711 pKa = 3.34QLLAVIDD718 pKa = 4.37GAAKK722 pKa = 9.73NQPQVGVIGQIQCPTVKK739 pKa = 10.44DD740 pKa = 4.19AIEE743 pKa = 4.19MSDD746 pKa = 3.72NCLMAPNSCQCNPTGDD762 pKa = 4.25TDD764 pKa = 4.06SCDD767 pKa = 3.57CSEE770 pKa = 4.3VSLASGFQSEE780 pKa = 4.62NQLPLHH786 pKa = 6.67IGGDD790 pKa = 4.11YY791 pKa = 9.34LTQARR796 pKa = 11.84DD797 pKa = 3.45IPVLKK802 pKa = 10.67ASAYY806 pKa = 8.61GSALIRR812 pKa = 11.84VHH814 pKa = 6.33ATGTLISDD822 pKa = 3.7YY823 pKa = 11.38AEE825 pKa = 4.12EE826 pKa = 4.46EE827 pKa = 4.11EE828 pKa = 5.27CKK830 pKa = 9.65ITMGKK835 pKa = 10.37LNGCHH840 pKa = 6.09SCITGANLTYY850 pKa = 10.08TCNTAKK856 pKa = 10.09PASVVMKK863 pKa = 10.77CGGDD867 pKa = 3.32LNLILDD873 pKa = 4.93CDD875 pKa = 4.09SQDD878 pKa = 3.22KK879 pKa = 10.5QRR881 pKa = 11.84VARR884 pKa = 11.84VHH886 pKa = 6.22IPSPIIDD893 pKa = 3.78VACQTTCSKK902 pKa = 10.92EE903 pKa = 3.9KK904 pKa = 10.73LRR906 pKa = 11.84LTGVLKK912 pKa = 10.85SVGLSAVNNGSHH924 pKa = 6.15IQLIPSEE931 pKa = 4.4SMTDD935 pKa = 3.08LGSWLKK941 pKa = 10.91SIWAYY946 pKa = 9.6FSWWYY951 pKa = 8.72TFVVALIILMIACLLLYY968 pKa = 10.17GFHH971 pKa = 6.87KK972 pKa = 10.64FKK974 pKa = 11.03VAFIEE979 pKa = 4.15KK980 pKa = 9.61QKK982 pKa = 11.16VYY984 pKa = 11.12

Molecular weight: 109.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5VGS1|W5VGS1_9VIRU Replicase OS=Cumuto virus OX=1457166 PE=4 SV=1
MM1 pKa = 7.49SIVPTVDD8 pKa = 3.32DD9 pKa = 3.53QLVAEE14 pKa = 5.58GIISDD19 pKa = 4.91LADD22 pKa = 3.86DD23 pKa = 4.99VINDD27 pKa = 3.21STLAVKK33 pKa = 10.29SINLAYY39 pKa = 10.45QGFDD43 pKa = 3.15PVYY46 pKa = 10.3LMQVLAYY53 pKa = 9.45RR54 pKa = 11.84ARR56 pKa = 11.84DD57 pKa = 3.62AKK59 pKa = 10.58ISAVNHH65 pKa = 5.85KK66 pKa = 11.01SNLRR70 pKa = 11.84TLALIGTMRR79 pKa = 11.84GSKK82 pKa = 9.82IEE84 pKa = 4.33TISGRR89 pKa = 11.84SGQEE93 pKa = 3.48LKK95 pKa = 11.12DD96 pKa = 3.81FLVKK100 pKa = 10.06MVRR103 pKa = 11.84DD104 pKa = 3.85YY105 pKa = 11.2KK106 pKa = 10.84LKK108 pKa = 10.5SGRR111 pKa = 11.84PTSNQDD117 pKa = 2.68ATLLRR122 pKa = 11.84IAAIYY127 pKa = 8.29AAPLAIAIKK136 pKa = 10.09NNAVSITTTIAPNNVAPGYY155 pKa = 9.47PRR157 pKa = 11.84FMCLSTFGALIPSAEE172 pKa = 4.15SMRR175 pKa = 11.84EE176 pKa = 3.93GEE178 pKa = 4.08QALIAGAFAEE188 pKa = 4.41HH189 pKa = 6.1QALFDD194 pKa = 3.67RR195 pKa = 11.84VINSRR200 pKa = 11.84TKK202 pKa = 9.72NYY204 pKa = 10.27SPKK207 pKa = 10.69DD208 pKa = 3.54VIKK211 pKa = 10.83NYY213 pKa = 10.07IQIQQLSALYY223 pKa = 10.66SEE225 pKa = 4.91GDD227 pKa = 3.56RR228 pKa = 11.84IKK230 pKa = 10.14TLLALGLIEE239 pKa = 4.32MMGNQMYY246 pKa = 10.07QVTALYY252 pKa = 10.39RR253 pKa = 11.84SALNAAKK260 pKa = 10.14QKK262 pKa = 9.74WDD264 pKa = 3.25ARR266 pKa = 11.84VV267 pKa = 2.9

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3322

267

2071

1107.3

126.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.877 ± 1.932

2.348 ± 1.511

5.81 ± 0.268

6.893 ± 1.277

3.793 ± 0.504

5.027 ± 0.819

1.836 ± 0.422

7.164 ± 0.519

7.556 ± 0.602

9.482 ± 0.864

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.402 ± 0.122

4.846 ± 0.185

3.402 ± 0.315

3.642 ± 0.316

4.967 ± 0.891

8.158 ± 0.477

5.418 ± 0.317

6.412 ± 0.754

1.595 ± 0.353

3.371 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski