Salipiger profundus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria;

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7D0E7|A0A1U7D0E7_9RHOB Putative amidohydrolase OS=Salipiger profundus OX=1229727 GN=Ga0080559_TMP833 PE=4 SV=1
MM1 pKa = 7.6ALAPVLIRR9 pKa = 11.84APGTGIGQILGRR21 pKa = 11.84DD22 pKa = 3.52PAVNDD27 pKa = 3.6GTLFAFDD34 pKa = 5.28FADD37 pKa = 4.47TYY39 pKa = 10.99CWPDD43 pKa = 3.32QASAPDD49 pKa = 3.68GSEE52 pKa = 4.24FINLIDD58 pKa = 3.63SGGSAFVNTSGPAIGFFDD76 pKa = 4.58DD77 pKa = 4.17GFRR80 pKa = 11.84YY81 pKa = 9.85NVGATDD87 pKa = 3.99QVIDD91 pKa = 4.99LPDD94 pKa = 3.38EE95 pKa = 4.31TKK97 pKa = 10.52FAASTGFVVSVVITIEE113 pKa = 4.19TQTQAAKK120 pKa = 10.72SRR122 pKa = 11.84ICGYY126 pKa = 10.79FDD128 pKa = 4.78GSDD131 pKa = 3.29NTGAWGLYY139 pKa = 5.39TANGRR144 pKa = 11.84FYY146 pKa = 10.82FVINGQVMPSSYY158 pKa = 10.57TIATFGTGPHH168 pKa = 6.4TFTLSWADD176 pKa = 3.22EE177 pKa = 4.2GDD179 pKa = 3.73GYY181 pKa = 11.16KK182 pKa = 10.58CKK184 pKa = 10.59LYY186 pKa = 11.1VDD188 pKa = 4.09GTLDD192 pKa = 4.15QEE194 pKa = 4.48ADD196 pKa = 3.46ADD198 pKa = 4.05NATLNTPTDD207 pKa = 3.53TTTPTLGEE215 pKa = 4.17SSDD218 pKa = 5.52DD219 pKa = 3.28EE220 pKa = 4.38TAPYY224 pKa = 9.43WIGKK228 pKa = 7.35ICRR231 pKa = 11.84CIGHH235 pKa = 7.25DD236 pKa = 3.68LASTEE241 pKa = 4.21LTVADD246 pKa = 3.7IAAAEE251 pKa = 4.08WAVTDD256 pKa = 3.96GRR258 pKa = 11.84FVAA261 pKa = 5.24

Molecular weight: 27.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7D568|A0A1U7D568_9RHOB Coenzyme A biosynthesis bifunctional protein CoaBC OS=Salipiger profundus OX=1229727 GN=coaBC PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44AGRR28 pKa = 11.84KK29 pKa = 8.46VLNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.51QLSAA44 pKa = 3.97

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5105

0

5105

1558204

37

2151

305.2

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.526 ± 0.041

0.893 ± 0.01

6.107 ± 0.034

6.319 ± 0.033

3.578 ± 0.023

8.971 ± 0.042

2.035 ± 0.017

4.811 ± 0.025

2.715 ± 0.027

10.159 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.782 ± 0.018

2.307 ± 0.018

5.258 ± 0.026

2.979 ± 0.015

7.155 ± 0.042

5.096 ± 0.021

5.476 ± 0.027

7.256 ± 0.026

1.426 ± 0.015

2.15 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski