Olleya namhaensis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Olleya

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3KYY8|A0A1I3KYY8_9FLAO Protein involved in gliding motility GldK OS=Olleya namhaensis OX=1144750 GN=SAMN05443431_102167 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.3KK3 pKa = 10.39VLVLLLSLSLFTACNDD19 pKa = 3.64EE20 pKa = 4.04PVEE23 pKa = 4.44GYY25 pKa = 9.67FAALDD30 pKa = 3.88SDD32 pKa = 4.01EE33 pKa = 4.12GTIPGNTSDD42 pKa = 3.91EE43 pKa = 4.51LKK45 pKa = 10.46LASYY49 pKa = 9.26TYY51 pKa = 10.59DD52 pKa = 3.39VEE54 pKa = 4.52TEE56 pKa = 4.08APFIGPLILNSDD68 pKa = 4.11FNFNDD73 pKa = 3.51DD74 pKa = 4.11NKK76 pKa = 10.39VNTLNVEE83 pKa = 4.71TIAFGFPFTTTGTVIRR99 pKa = 11.84DD100 pKa = 3.47TDD102 pKa = 3.71GKK104 pKa = 8.92ITAIQTFDD112 pKa = 3.03GSALVSQTDD121 pKa = 3.1IFYY124 pKa = 10.78SAGNTISQITHH135 pKa = 7.46DD136 pKa = 5.01DD137 pKa = 3.61FDD139 pKa = 6.9DD140 pKa = 3.92NAEE143 pKa = 4.34DD144 pKa = 3.58YY145 pKa = 10.05TFNFTTLNNQITKK158 pKa = 9.08TEE160 pKa = 4.3PGNPVTTVYY169 pKa = 9.88TADD172 pKa = 3.73GSGKK176 pKa = 10.66LIDD179 pKa = 4.09KK180 pKa = 10.85SSFEE184 pKa = 4.34AGEE187 pKa = 4.56LIQSEE192 pKa = 4.46ILTYY196 pKa = 10.47DD197 pKa = 3.59ANDD200 pKa = 3.14NCISVVSTGEE210 pKa = 3.99FNNTTTYY217 pKa = 11.24GFDD220 pKa = 3.47SFSNPLKK227 pKa = 10.94DD228 pKa = 4.36GFSDD232 pKa = 4.25QYY234 pKa = 10.06WLSILDD240 pKa = 4.42DD241 pKa = 4.74DD242 pKa = 5.3YY243 pKa = 12.02EE244 pKa = 6.1DD245 pKa = 3.68EE246 pKa = 4.89AGPAIVQFHH255 pKa = 6.87GSNNWNSVSSEE266 pKa = 3.96GTTVTLMINYY276 pKa = 9.29DD277 pKa = 3.43STNRR281 pKa = 11.84ITSRR285 pKa = 11.84SGVFSLSGIEE295 pKa = 4.09IVQEE299 pKa = 3.73EE300 pKa = 4.57AFNYY304 pKa = 9.75VNN306 pKa = 4.09

Molecular weight: 33.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3PD91|A0A1I3PD91_9FLAO RNA polymerase sigma factor OS=Olleya namhaensis OX=1144750 GN=SAMN05443431_10569 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.92GRR40 pKa = 11.84KK41 pKa = 8.32KK42 pKa = 10.28ISVSTEE48 pKa = 3.6TRR50 pKa = 11.84HH51 pKa = 6.44KK52 pKa = 10.57KK53 pKa = 9.81

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3170

0

3170

1067814

29

3043

336.8

37.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.52 ± 0.048

0.764 ± 0.018

5.96 ± 0.04

6.027 ± 0.043

5.216 ± 0.037

6.115 ± 0.046

1.675 ± 0.019

7.952 ± 0.041

7.838 ± 0.063

9.389 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.022

6.485 ± 0.053

3.181 ± 0.024

3.533 ± 0.026

2.993 ± 0.029

6.453 ± 0.037

6.604 ± 0.064

6.307 ± 0.038

0.96 ± 0.015

4.046 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski