Sediminicola luteus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A4GAC9|A0A2A4GAC9_9FLAO Galactokinase OS=Sediminicola luteus OX=319238 GN=B7P33_05890 PE=3 SV=1
MM1 pKa = 7.4 EE2 pKa = 5.18 SKK4 pKa = 10.39 EE5 pKa = 3.94 KK6 pKa = 10.82 AMKK9 pKa = 9.97 KK10 pKa = 10.4 LNISTYY16 pKa = 10.49 VIALVLLVLALAVFMGCEE34 pKa = 4.36 PDD36 pKa = 5.11 DD37 pKa = 4.4 SDD39 pKa = 5.47 AFPASLPDD47 pKa = 3.45 TAAVFTDD54 pKa = 4.6 GFSPGLDD61 pKa = 3.13 YY62 pKa = 10.89 TAFGDD67 pKa = 4.01 SKK69 pKa = 10.4 VTAFQLDD76 pKa = 3.76 EE77 pKa = 4.13 EE78 pKa = 4.62 VTYY81 pKa = 10.92 DD82 pKa = 3.88 KK83 pKa = 11.1 SDD85 pKa = 2.83 SAMRR89 pKa = 11.84 FDD91 pKa = 5.49 IPNQGDD97 pKa = 3.89 PEE99 pKa = 4.24 SGYY102 pKa = 11.37 AGGIFFDD109 pKa = 3.31 KK110 pKa = 10.5 TGRR113 pKa = 11.84 NLSGYY118 pKa = 9.89 NVLSFWGKK126 pKa = 9.88 ASQGAIIQEE135 pKa = 4.31 LGFGLTDD142 pKa = 3.2 TQMYY146 pKa = 6.86 RR147 pKa = 11.84 TSITDD152 pKa = 3.38 VAFSTAWTKK161 pKa = 11.06 YY162 pKa = 10.25 YY163 pKa = 10.33 ILLPDD168 pKa = 4.18 PARR171 pKa = 11.84 LTMEE175 pKa = 3.78 EE176 pKa = 4.28 GMFYY180 pKa = 10.59 FVDD183 pKa = 3.81 TPDD186 pKa = 3.24 NGKK189 pKa = 10.73 GYY191 pKa = 9.99 SFWIDD196 pKa = 3.06 EE197 pKa = 4.32 LRR199 pKa = 11.84 FEE201 pKa = 4.34 NSGTIGQVQAYY212 pKa = 7.73 IQNGEE217 pKa = 4.35 EE218 pKa = 4.07 VTSTVFVGQEE228 pKa = 3.99 VPVSGLGVSFSLPNGVSQAVNAAPAFFTLASSNPQVAQVVVDD270 pKa = 3.61 EE271 pKa = 4.55 TTQVSTVRR279 pKa = 11.84 ILAQGEE285 pKa = 4.2 AVITASMGDD294 pKa = 3.66 VAAMGSLTLQVRR306 pKa = 11.84 EE307 pKa = 4.59 FEE309 pKa = 4.41 PAPLPDD315 pKa = 3.99 KK316 pKa = 11.06 VADD319 pKa = 3.87 DD320 pKa = 4.54 VISIFSDD327 pKa = 3.34 AYY329 pKa = 9.32 TNVPVDD335 pKa = 3.97 FYY337 pKa = 11.44 NGYY340 pKa = 9.09 YY341 pKa = 10.63 APFQTTVSADD351 pKa = 3.39 FTVDD355 pKa = 3.05 GDD357 pKa = 4.24 HH358 pKa = 6.34 VLNYY362 pKa = 10.05 INYY365 pKa = 8.29 NFVGIEE371 pKa = 3.99 FNKK374 pKa = 10.07 EE375 pKa = 3.72 VPTIDD380 pKa = 3.42 ATEE383 pKa = 4.08 MTHH386 pKa = 6.19 LHH388 pKa = 5.27 VDD390 pKa = 3.55 IYY392 pKa = 10.7 IPEE395 pKa = 4.4 ALDD398 pKa = 4.25 PNATWRR404 pKa = 11.84 VNLRR408 pKa = 11.84 DD409 pKa = 4.25 FGADD413 pKa = 3.27 GNFDD417 pKa = 3.99 GGDD420 pKa = 3.53 DD421 pKa = 4.27 TIASQLLTTASDD433 pKa = 3.64 PALVSEE439 pKa = 4.26 QWISVDD445 pKa = 2.97 MDD447 pKa = 3.04 IRR449 pKa = 11.84 DD450 pKa = 3.91 MANKK454 pKa = 8.38 ATLGQIVFDD463 pKa = 4.57 AEE465 pKa = 4.79 GDD467 pKa = 3.72 TSPRR471 pKa = 11.84 PSGFYY476 pKa = 10.51 VDD478 pKa = 5.44 NLYY481 pKa = 10.86 LYY483 pKa = 10.38 NDD485 pKa = 3.48 QGTGGGGPQPIPPTTAAPTPTLDD508 pKa = 3.17 AAQVVSIFSDD518 pKa = 3.58 AYY520 pKa = 9.95 TDD522 pKa = 3.82 VPNQGFNQYY531 pKa = 9.31 GAATFAEE538 pKa = 4.66 IPVADD543 pKa = 4.23 NATLNYY549 pKa = 9.04 KK550 pKa = 10.35 QSDD553 pKa = 3.83 NPGGNFQVIEE563 pKa = 4.47 LGAEE567 pKa = 3.95 NQIDD571 pKa = 3.9 ATAMEE576 pKa = 4.64 LTDD579 pKa = 3.72 FHH581 pKa = 8.03 VDD583 pKa = 2.64 LWFPNPVNEE592 pKa = 4.07 NTAFLLKK599 pKa = 10.24 VVNIAPDD606 pKa = 3.33 ITSEE610 pKa = 3.79 ALIRR614 pKa = 11.84 IDD616 pKa = 5.23 ANATPAMAQGQWLAIDD632 pKa = 3.94 LTMDD636 pKa = 3.3 QLQAAGLAANSNIQQVVIDD655 pKa = 4.87 LLDD658 pKa = 4.13 AGEE661 pKa = 4.27 VYY663 pKa = 10.34 IDD665 pKa = 3.4 NLYY668 pKa = 10.45 FSKK671 pKa = 11.26 SNGAGGSAPTTSAPEE686 pKa = 3.92 PPARR690 pKa = 11.84 DD691 pKa = 3.32 AADD694 pKa = 3.66 VISIFGEE701 pKa = 4.22 AYY703 pKa = 9.89 TNITGIDD710 pKa = 3.83 YY711 pKa = 10.72 DD712 pKa = 4.56 PNWGQSGHH720 pKa = 5.26 MQVNTAFDD728 pKa = 3.68 SGDD731 pKa = 3.45 GNLALAYY738 pKa = 9.71 PSFNYY743 pKa = 10.11 QGTDD747 pKa = 3.54 FSGNAQNASEE757 pKa = 4.28 MEE759 pKa = 4.21 FLHH762 pKa = 7.81 VDD764 pKa = 3.06 IWVPAGTDD772 pKa = 3.22 RR773 pKa = 11.84 QVKK776 pKa = 9.6 VSPINNGTGEE786 pKa = 4.33 GEE788 pKa = 4.4 VLVSVPLIPGSWNSVDD804 pKa = 5.51 LPIADD809 pKa = 3.98 FTGMTWDD816 pKa = 3.94 SVVQLKK822 pKa = 10.5 FDD824 pKa = 3.7 GQFNGDD830 pKa = 3.11 GSANTDD836 pKa = 3.64 PFDD839 pKa = 3.62 MYY841 pKa = 11.25 LDD843 pKa = 3.25 NVYY846 pKa = 10.17 FYY848 pKa = 10.44 KK849 pKa = 10.75 EE850 pKa = 3.86 PSAPSAAPTTSAPEE864 pKa = 3.83 PLARR868 pKa = 11.84 DD869 pKa = 3.38 AADD872 pKa = 3.59 VISIFGEE879 pKa = 4.22 AYY881 pKa = 9.89 TNITGIDD888 pKa = 3.83 YY889 pKa = 10.72 DD890 pKa = 4.56 PNWGQSGHH898 pKa = 5.26 MQVNTAFDD906 pKa = 4.3 PGDD909 pKa = 3.75 GNLALAYY916 pKa = 9.71 PSFNYY921 pKa = 10.11 QGTDD925 pKa = 3.54 FSGNAQNASEE935 pKa = 4.28 MEE937 pKa = 4.21 FLHH940 pKa = 7.81 VDD942 pKa = 3.06 IWVPAGTDD950 pKa = 3.22 RR951 pKa = 11.84 QVKK954 pKa = 9.6 VSPINNGTGEE964 pKa = 4.33 GEE966 pKa = 4.4 VLVSVPLTPGAWNSVDD982 pKa = 5.21 LPMADD987 pKa = 3.63 FTGMTWDD994 pKa = 3.94 SVVQLKK1000 pKa = 10.5 FDD1002 pKa = 3.7 GQFNGDD1008 pKa = 3.11 GSANTDD1014 pKa = 3.31 PFDD1017 pKa = 3.99 IYY1019 pKa = 11.05 LDD1021 pKa = 3.23 NVYY1024 pKa = 10.14 FYY1026 pKa = 10.44 KK1027 pKa = 10.7 EE1028 pKa = 3.75 PSAPSTAPTTSAPAPPVRR1046 pKa = 11.84 DD1047 pKa = 3.33 AADD1050 pKa = 3.61 VISIFGEE1057 pKa = 4.22 AYY1059 pKa = 9.89 TNITGIDD1066 pKa = 3.83 YY1067 pKa = 10.72 DD1068 pKa = 4.56 PNWGQSGHH1076 pKa = 5.26 MQVNTAFDD1084 pKa = 4.3 PGDD1087 pKa = 3.75 GNLALAYY1094 pKa = 9.71 PSFNYY1099 pKa = 10.11 QGTDD1103 pKa = 3.54 FSGNAQNAATMQFLHH1118 pKa = 7.2 VDD1120 pKa = 3.2 IWVPAGTDD1128 pKa = 3.22 RR1129 pKa = 11.84 QVKK1132 pKa = 9.6 VSPINNGTGAGEE1144 pKa = 4.21 VLVSVPLTPGAWNSVDD1160 pKa = 4.95 LPIGDD1165 pKa = 4.06 FTGMTWDD1172 pKa = 4.28 SVFQLKK1178 pKa = 10.32 FDD1180 pKa = 3.85 GQFNGDD1186 pKa = 3.11 GSANTDD1192 pKa = 3.31 PFDD1195 pKa = 3.99 IYY1197 pKa = 11.05 LDD1199 pKa = 3.23 NVYY1202 pKa = 10.14 FYY1204 pKa = 10.44 KK1205 pKa = 10.7 EE1206 pKa = 3.75 PSAPSTAPTTSAPAPPVRR1224 pKa = 11.84 DD1225 pKa = 3.33 AADD1228 pKa = 3.61 VISIFGEE1235 pKa = 4.22 AYY1237 pKa = 9.67 TNITGVDD1244 pKa = 3.77 YY1245 pKa = 11.05 DD1246 pKa = 4.63 PNWGQSGHH1254 pKa = 5.26 MQVNTAFDD1262 pKa = 4.3 PGDD1265 pKa = 3.75 GNLALAYY1272 pKa = 9.89 PNFNYY1277 pKa = 10.28 QGTDD1281 pKa = 3.53 FSGNAQNAASMQFLHH1296 pKa = 7.39 VDD1298 pKa = 3.07 IWVPAGTDD1306 pKa = 3.22 RR1307 pKa = 11.84 QVKK1310 pKa = 9.6 VSPINNGTGAGEE1322 pKa = 4.21 VLVSVPLTPGAWNSVDD1338 pKa = 4.95 LPIGDD1343 pKa = 4.06 FTGMTWDD1350 pKa = 4.28 SVFQLKK1356 pKa = 10.32 FDD1358 pKa = 3.85 GQFNGDD1364 pKa = 3.11 GSANTDD1370 pKa = 3.42 PFDD1373 pKa = 3.73 VYY1375 pKa = 11.29 LDD1377 pKa = 3.31 NVYY1380 pKa = 10.22 FYY1382 pKa = 11.57 KK1383 pKa = 10.9 NN1384 pKa = 3.04
Molecular weight: 148.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.656
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.668
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 0.998
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.748
Protein with the highest isoelectric point:
>tr|A0A2A4G414|A0A2A4G414_9FLAO Uncharacterized protein OS=Sediminicola luteus OX=319238 GN=B7P33_15560 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 10.15 RR10 pKa = 11.84 KK11 pKa = 9.6 RR12 pKa = 11.84 KK13 pKa = 8.29 NKK15 pKa = 9.34 HH16 pKa = 4.03 GFRR19 pKa = 11.84 EE20 pKa = 4.2 RR21 pKa = 11.84 MASANGRR28 pKa = 11.84 KK29 pKa = 9.04 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.09 GRR39 pKa = 11.84 KK40 pKa = 7.97 KK41 pKa = 10.66 LSVSSEE47 pKa = 3.9 LRR49 pKa = 11.84 HH50 pKa = 6.16 KK51 pKa = 10.53 KK52 pKa = 10.04
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.457
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.296
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 12.018
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.041
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3771
0
3771
1364760
31
6537
361.9
40.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.441 ± 0.054
0.756 ± 0.015
5.85 ± 0.049
6.286 ± 0.043
4.905 ± 0.029
7.306 ± 0.04
2.093 ± 0.021
6.441 ± 0.036
6.523 ± 0.06
9.711 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.024
5.083 ± 0.047
3.978 ± 0.026
3.978 ± 0.025
3.981 ± 0.034
5.952 ± 0.031
5.814 ± 0.064
6.313 ± 0.032
1.261 ± 0.014
3.941 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here