Mycoplasma edwardii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1362 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B0PNP5|A0A3B0PNP5_9MOLU Elongation factor Ts (Fragment) OS=Mycoplasma edwardii OX=53558 GN=NCTC10132_00792 PE=3 SV=1
MM1 pKa = 7.27GVSPLGVVASEE12 pKa = 3.96PSVFVGGVVVPDD24 pKa = 4.89DD25 pKa = 4.51VDD27 pKa = 3.99PPCAPSFVVGFEE39 pKa = 3.91GSAVDD44 pKa = 4.73VEE46 pKa = 4.32PSLLGVGAAGCGCGLDD62 pKa = 3.98DD63 pKa = 5.43VSPLLVGFDD72 pKa = 3.54ASGEE76 pKa = 4.45VVSSAMIVVPGPCCFSSFFTSLFVDD101 pKa = 3.39EE102 pKa = 5.69LGFSLFLSLKK112 pKa = 10.11LHH114 pKa = 6.5DD115 pKa = 3.79TAEE118 pKa = 4.04IANADD123 pKa = 4.07VIDD126 pKa = 3.86KK127 pKa = 11.03LKK129 pKa = 11.08VEE131 pKa = 4.87NIDD134 pKa = 3.51FHH136 pKa = 7.87FLIILIPLFLL146 pKa = 5.83

Molecular weight: 15.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B0PMF4|A0A3B0PMF4_9MOLU Uncharacterized protein (Fragment) OS=Mycoplasma edwardii OX=53558 GN=NCTC10132_00332 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.78LCGRR29 pKa = 11.84PHH31 pKa = 6.83SVLRR35 pKa = 11.84KK36 pKa = 9.13YY37 pKa = 10.01KK38 pKa = 9.42VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.89EE50 pKa = 4.48GKK52 pKa = 10.19IPGVKK57 pKa = 9.38KK58 pKa = 10.89ASWW61 pKa = 3.03

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1362

0

1362

180421

29

1275

132.5

15.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.442 ± 0.09

0.381 ± 0.016

5.624 ± 0.071

7.3 ± 0.094

5.334 ± 0.09

4.715 ± 0.086

1.431 ± 0.033

9.125 ± 0.085

10.08 ± 0.083

9.47 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.042

7.729 ± 0.106

2.681 ± 0.058

3.081 ± 0.052

3.071 ± 0.054

6.934 ± 0.075

5.102 ± 0.061

5.857 ± 0.069

0.562 ± 0.017

3.875 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski