Sendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Average proteome isoelectric point is 7.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P04862-2|C-2_SENDZ Isoform of P04862 Isoform C protein of C' protein OS=Sendai virus (strain Z) OX=11198 GN=P/V/C PE=1 SV=3
MM1 pKa = 7.56 DD2 pKa = 3.68 QDD4 pKa = 4.41 AFILKK9 pKa = 9.71 EE10 pKa = 3.88 DD11 pKa = 4.02 SEE13 pKa = 4.98 VEE15 pKa = 3.95 RR16 pKa = 11.84 EE17 pKa = 4.15 APGGRR22 pKa = 11.84 EE23 pKa = 3.99 SLSDD27 pKa = 3.58 VIGFLDD33 pKa = 3.71 AVLSSEE39 pKa = 4.09 PTDD42 pKa = 3.29 IGGDD46 pKa = 3.55 RR47 pKa = 11.84 SWLHH51 pKa = 5.09 NTINTPQGPGSAHH64 pKa = 6.25 RR65 pKa = 11.84 AKK67 pKa = 10.78 SEE69 pKa = 4.15 GEE71 pKa = 4.42 GEE73 pKa = 4.28 VSTPSTQDD81 pKa = 2.88 NRR83 pKa = 11.84 SGEE86 pKa = 4.16 EE87 pKa = 3.75 SRR89 pKa = 11.84 VSGRR93 pKa = 11.84 TSKK96 pKa = 11.09 PEE98 pKa = 3.94 AEE100 pKa = 3.93 AHH102 pKa = 6.43 AGNLDD107 pKa = 3.26 KK108 pKa = 11.5 QNIHH112 pKa = 6.5 RR113 pKa = 11.84 AFGGRR118 pKa = 11.84 TGTNSVSQDD127 pKa = 3.37 LGDD130 pKa = 4.65 GGDD133 pKa = 3.67 SGILEE138 pKa = 4.43 NPPNEE143 pKa = 3.91 RR144 pKa = 11.84 GYY146 pKa = 9.84 PRR148 pKa = 11.84 SGIEE152 pKa = 3.92 DD153 pKa = 3.78 EE154 pKa = 4.2 NRR156 pKa = 11.84 EE157 pKa = 4.11 MAAHH161 pKa = 6.69 PDD163 pKa = 3.54 KK164 pKa = 11.08 RR165 pKa = 11.84 GEE167 pKa = 4.12 DD168 pKa = 3.56 QAEE171 pKa = 4.07 GLPEE175 pKa = 4.03 EE176 pKa = 4.6 VRR178 pKa = 11.84 GSTSLPDD185 pKa = 3.6 EE186 pKa = 4.86 GEE188 pKa = 4.37 GGASNNGRR196 pKa = 11.84 SMEE199 pKa = 4.61 PGSSHH204 pKa = 5.66 SARR207 pKa = 11.84 VTGVLVIPSPEE218 pKa = 3.91 LEE220 pKa = 4.07 EE221 pKa = 4.42 AVLRR225 pKa = 11.84 RR226 pKa = 11.84 NKK228 pKa = 9.86 RR229 pKa = 11.84 RR230 pKa = 11.84 PTNSGSKK237 pKa = 9.48 PLTPATVPGTRR248 pKa = 11.84 SPPLNRR254 pKa = 11.84 YY255 pKa = 8.99 NSTGSPPGKK264 pKa = 9.58 PPSTQDD270 pKa = 2.94 EE271 pKa = 5.06 HH272 pKa = 8.35 INSGDD277 pKa = 3.51 TPAVRR282 pKa = 11.84 VKK284 pKa = 10.65 DD285 pKa = 4.02 RR286 pKa = 11.84 KK287 pKa = 9.93 PPIGTRR293 pKa = 11.84 SVSDD297 pKa = 3.76 CPANGRR303 pKa = 11.84 SIHH306 pKa = 6.83 PGLEE310 pKa = 3.79 TDD312 pKa = 3.74 STKK315 pKa = 10.79 KK316 pKa = 10.53 GAA318 pKa = 3.75
Molecular weight: 33.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.183
IPC2_protein 5.118
IPC_protein 5.067
Toseland 5.029
ProMoST 5.207
Dawson 5.054
Bjellqvist 5.181
Wikipedia 4.927
Rodwell 4.978
Grimsley 4.952
Solomon 5.054
Lehninger 5.016
Nozaki 5.181
DTASelect 5.321
Thurlkill 5.029
EMBOSS 4.978
Sillero 5.258
Patrickios 4.558
IPC_peptide 5.067
IPC2_peptide 5.258
IPC2.peptide.svr19 5.235
Protein with the highest isoelectric point:
>sp|P69282|V_SENDZ Protein V OS=Sendai virus (strain Z) OX=11198 GN=P/V/C PE=1 SV=1
MM1 pKa = 7.6 ADD3 pKa = 3.36 IYY5 pKa = 10.85 RR6 pKa = 11.84 FPKK9 pKa = 10.59 FSYY12 pKa = 9.74 EE13 pKa = 4.12 DD14 pKa = 3.44 NGTVEE19 pKa = 4.59 PLPLRR24 pKa = 11.84 TGPDD28 pKa = 3.01 KK29 pKa = 10.99 KK30 pKa = 10.56 AIPHH34 pKa = 5.1 IRR36 pKa = 11.84 IVKK39 pKa = 10.01 VGDD42 pKa = 3.63 PPKK45 pKa = 10.69 HH46 pKa = 4.84 GVRR49 pKa = 11.84 YY50 pKa = 10.03 LDD52 pKa = 3.96 LLLLGFFEE60 pKa = 4.98 TPKK63 pKa = 9.37 QTTNLGSVSDD73 pKa = 3.87 LTEE76 pKa = 3.94 PTSYY80 pKa = 10.56 SICGSGSLPIGVAKK94 pKa = 10.76 YY95 pKa = 10.52 YY96 pKa = 9.98 GTDD99 pKa = 3.29 QEE101 pKa = 4.62 LLKK104 pKa = 11.01 ACTDD108 pKa = 3.21 LRR110 pKa = 11.84 ITVRR114 pKa = 11.84 RR115 pKa = 11.84 TVRR118 pKa = 11.84 AGEE121 pKa = 4.17 MIVYY125 pKa = 8.62 MVDD128 pKa = 3.46 SIGAPLLPWSGRR140 pKa = 11.84 LRR142 pKa = 11.84 QGMIFNANKK151 pKa = 9.77 VALAPQCLPVDD162 pKa = 3.24 KK163 pKa = 10.48 DD164 pKa = 2.85 IRR166 pKa = 11.84 LRR168 pKa = 11.84 VVFVNGTSLGAITIAKK184 pKa = 9.25 IPKK187 pKa = 8.6 TLADD191 pKa = 3.79 LALPNSISVNLLVTLKK207 pKa = 10.0 TGISTEE213 pKa = 4.15 QKK215 pKa = 10.47 GVLPVLDD222 pKa = 4.08 DD223 pKa = 3.73 QGEE226 pKa = 4.28 KK227 pKa = 10.49 KK228 pKa = 10.88 LNFMVHH234 pKa = 6.52 LGLIRR239 pKa = 11.84 RR240 pKa = 11.84 KK241 pKa = 9.27 VGKK244 pKa = 9.46 IYY246 pKa = 10.67 SVEE249 pKa = 4.02 YY250 pKa = 9.84 CKK252 pKa = 11.04 SKK254 pKa = 10.18 IEE256 pKa = 3.93 RR257 pKa = 11.84 MRR259 pKa = 11.84 LIFSLGLIGGISFHH273 pKa = 5.57 VQVNGTLSKK282 pKa = 10.14 TFMSQLAWKK291 pKa = 10.01 RR292 pKa = 11.84 AVCFPLMDD300 pKa = 4.24 VNPHH304 pKa = 4.81 MNMVIWAASVEE315 pKa = 4.3 ITGVDD320 pKa = 3.49 AVFQPAIPRR329 pKa = 11.84 DD330 pKa = 3.38 FRR332 pKa = 11.84 YY333 pKa = 10.38 YY334 pKa = 10.21 PNVVAKK340 pKa = 10.68 NIGRR344 pKa = 11.84 IRR346 pKa = 11.84 KK347 pKa = 8.53 LL348 pKa = 3.16
Molecular weight: 38.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.094
IPC2_protein 9.092
IPC_protein 9.019
Toseland 9.794
ProMoST 9.487
Dawson 10.014
Bjellqvist 9.706
Wikipedia 10.175
Rodwell 10.423
Grimsley 10.087
Solomon 10.043
Lehninger 9.999
Nozaki 9.853
DTASelect 9.677
Thurlkill 9.867
EMBOSS 10.204
Sillero 9.94
Patrickios 9.545
IPC_peptide 10.043
IPC2_peptide 8.346
IPC2.peptide.svr19 7.995
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
3
10
3475
175
568
347.5
38.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.216 ± 0.587
1.065 ± 0.286
5.295 ± 0.412
8.144 ± 1.137
1.957 ± 0.407
7.453 ± 0.844
1.871 ± 0.161
6.129 ± 0.866
5.928 ± 0.618
8.863 ± 0.88
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.561 ± 0.403
3.54 ± 0.482
5.468 ± 0.802
3.914 ± 0.553
7.367 ± 0.511
8.489 ± 0.923
6.647 ± 0.269
6.187 ± 0.631
0.806 ± 0.181
2.101 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here