Staphylococcus phage phi7401PVL

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; unclassified Triavirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R4WAU5|R4WAU5_9CAUD Uncharacterized protein OS=Staphylococcus phage phi7401PVL OX=1165702 PE=4 SV=1
MM1 pKa = 7.16EE2 pKa = 4.6TNKK5 pKa = 10.53TIDD8 pKa = 3.2LMNYY12 pKa = 8.65VEE14 pKa = 4.47FPKK17 pKa = 10.27RR18 pKa = 11.84YY19 pKa = 7.91TEE21 pKa = 4.18AKK23 pKa = 9.2GKK25 pKa = 10.18LVAQPITTINSARR38 pKa = 11.84RR39 pKa = 11.84VEE41 pKa = 4.14NEE43 pKa = 4.14DD44 pKa = 3.04MTVCYY49 pKa = 10.28ILDD52 pKa = 3.58QDD54 pKa = 4.56DD55 pKa = 4.48DD56 pKa = 4.76VMDD59 pKa = 5.33FIFDD63 pKa = 3.65RR64 pKa = 11.84DD65 pKa = 3.95IITVYY70 pKa = 10.68CPEE73 pKa = 4.71NGTATDD79 pKa = 4.09EE80 pKa = 4.34YY81 pKa = 10.38FCEE84 pKa = 4.86IIFNSDD90 pKa = 3.11DD91 pKa = 3.54TFTLKK96 pKa = 10.7RR97 pKa = 11.84LSNYY101 pKa = 7.23VTIKK105 pKa = 10.6DD106 pKa = 3.33RR107 pKa = 11.84SYY109 pKa = 11.57PMSKK113 pKa = 10.1INDD116 pKa = 3.27VNITGKK122 pKa = 8.66VVRR125 pKa = 11.84LFRR128 pKa = 11.84DD129 pKa = 5.26FKK131 pKa = 11.45

Molecular weight: 15.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R4WCU7|R4WCU7_9CAUD Uncharacterized protein OS=Staphylococcus phage phi7401PVL OX=1165702 PE=4 SV=1
MM1 pKa = 7.64ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.81VIRR8 pKa = 11.84VRR10 pKa = 11.84IKK12 pKa = 10.5GKK14 pKa = 10.6LMTLRR19 pKa = 11.84EE20 pKa = 4.12VSEE23 pKa = 4.8KK24 pKa = 10.69YY25 pKa = 10.32HH26 pKa = 6.64ISPEE30 pKa = 4.02LLRR33 pKa = 11.84YY34 pKa = 9.46RR35 pKa = 11.84YY36 pKa = 8.24KK37 pKa = 10.75HH38 pKa = 6.21KK39 pKa = 10.45MRR41 pKa = 11.84GDD43 pKa = 3.41EE44 pKa = 4.19LLCGRR49 pKa = 11.84KK50 pKa = 9.44DD51 pKa = 3.52SKK53 pKa = 11.56SKK55 pKa = 11.51DD56 pKa = 3.34EE57 pKa = 4.1VEE59 pKa = 4.23YY60 pKa = 9.76MQSQIKK66 pKa = 10.45DD67 pKa = 3.49EE68 pKa = 4.33EE69 pKa = 4.2KK70 pKa = 10.52GRR72 pKa = 11.84EE73 pKa = 4.07KK74 pKa = 10.34IRR76 pKa = 11.84KK77 pKa = 8.34KK78 pKa = 10.82AILNRR83 pKa = 11.84YY84 pKa = 5.97QRR86 pKa = 11.84NVRR89 pKa = 11.84AEE91 pKa = 3.88YY92 pKa = 8.71EE93 pKa = 4.07QEE95 pKa = 3.75RR96 pKa = 11.84KK97 pKa = 9.61RR98 pKa = 11.84RR99 pKa = 11.84LRR101 pKa = 11.84PWLYY105 pKa = 10.66DD106 pKa = 3.11GTPQKK111 pKa = 10.53HH112 pKa = 6.08SRR114 pKa = 11.84DD115 pKa = 4.06PYY117 pKa = 10.08WFDD120 pKa = 3.01VTYY123 pKa = 11.33NQMFKK128 pKa = 10.23KK129 pKa = 9.86WSEE132 pKa = 3.76AA133 pKa = 3.32

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

1

44

12760

82

2058

290.0

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.948 ± 0.613

0.455 ± 0.103

6.23 ± 0.292

7.375 ± 0.451

3.777 ± 0.218

6.34 ± 0.597

1.787 ± 0.138

6.873 ± 0.307

9.859 ± 0.546

8.119 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.155

6.599 ± 0.345

2.806 ± 0.234

3.683 ± 0.168

4.13 ± 0.246

6.489 ± 0.314

5.886 ± 0.263

5.58 ± 0.212

1.293 ± 0.124

4.295 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski