Pseudomonas alcaligenes (strain ATCC 14909 / DSM 50342 / JCM 20561 / NBRC 14159 / NCIMB 9945 / NCTC 10367 / 1577)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas aeruginosa group; Pseudomonas alcaligenes

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4528 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3B7U8|U3B7U8_PSEA4 ABC_trans_aux domain-containing protein OS=Pseudomonas alcaligenes (strain ATCC 14909 / DSM 50342 / JCM 20561 / NBRC 14159 / NCIMB 9945 / NCTC 10367 / 1577) OX=1215092 GN=PA6_017_00490 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 9.54TWICVICGLIYY13 pKa = 10.73DD14 pKa = 4.77EE15 pKa = 4.92ARR17 pKa = 11.84GWPDD21 pKa = 3.28DD22 pKa = 4.73GIPAGTPWAEE32 pKa = 4.5VPDD35 pKa = 4.5DD36 pKa = 4.54WLCPDD41 pKa = 4.66CGVSKK46 pKa = 11.1ADD48 pKa = 3.99FAMQEE53 pKa = 4.07LL54 pKa = 4.17

Molecular weight: 5.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2Z9R0|U2Z9R0_PSEA4 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] OS=Pseudomonas alcaligenes (strain ATCC 14909 / DSM 50342 / JCM 20561 / NBRC 14159 / NCIMB 9945 / NCTC 10367 / 1577) OX=1215092 GN=glmS PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4528

0

4528

1455039

44

3659

321.3

35.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.524 ± 0.048

1.024 ± 0.012

5.306 ± 0.037

6.193 ± 0.034

3.548 ± 0.019

7.989 ± 0.045

2.246 ± 0.018

4.426 ± 0.027

3.286 ± 0.033

12.448 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.019

2.727 ± 0.028

4.862 ± 0.027

4.785 ± 0.036

6.923 ± 0.038

5.506 ± 0.03

4.294 ± 0.033

6.766 ± 0.035

1.489 ± 0.018

2.527 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski