Amino acid dipepetide frequency for Pseudorhodoferax sp. Leaf267

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.616AlaAla: 20.616 ± 0.16	1.434AlaCys: 1.434 ± 0.031	7.137AlaAsp: 7.137 ± 0.077	6.853AlaGlu: 6.853 ± 0.067	4.215AlaPhe: 4.215 ± 0.051	12.033AlaGly: 12.033 ± 0.147	2.98AlaHis: 2.98 ± 0.045	5.24AlaIle: 5.24 ± 0.06	3.508AlaLys: 3.508 ± 0.051	16.922AlaLeu: 16.922 ± 0.135	3.844AlaMet: 3.844 ± 0.052	2.755AlaAsn: 2.755 ± 0.041	7.259AlaPro: 7.259 ± 0.077	6.834AlaGln: 6.834 ± 0.074	10.41AlaArg: 10.41 ± 0.091	7.175AlaSer: 7.175 ± 0.076	6.74AlaThr: 6.74 ± 0.083	9.807AlaVal: 9.807 ± 0.077	2.144AlaTrp: 2.144 ± 0.034	2.534AlaTyr: 2.534 ± 0.043	0.002AlaXaa: 0.002 ± 0.001
Cys	1.271CysAla: 1.271 ± 0.031	0.117CysCys: 0.117 ± 0.008	0.503CysAsp: 0.503 ± 0.016	0.479CysGlu: 0.479 ± 0.015	0.273CysPhe: 0.273 ± 0.012	0.972CysGly: 0.972 ± 0.023	0.258CysHis: 0.258 ± 0.011	0.389CysIle: 0.389 ± 0.014	0.209CysLys: 0.209 ± 0.009	0.79CysLeu: 0.79 ± 0.022	0.211CysMet: 0.211 ± 0.011	0.218CysAsn: 0.218 ± 0.009	0.433CysPro: 0.433 ± 0.018	0.266CysGln: 0.266 ± 0.012	0.575CysArg: 0.575 ± 0.017	0.485CysSer: 0.485 ± 0.015	0.524CysThr: 0.524 ± 0.018	0.73CysVal: 0.73 ± 0.022	0.137CysTrp: 0.137 ± 0.009	0.183CysTyr: 0.183 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.112AspAla: 8.112 ± 0.08	0.444AspCys: 0.444 ± 0.014	2.744AspAsp: 2.744 ± 0.044	2.834AspGlu: 2.834 ± 0.042	1.952AspPhe: 1.952 ± 0.033	4.91AspGly: 4.91 ± 0.07	1.121AspHis: 1.121 ± 0.023	2.346AspIle: 2.346 ± 0.036	1.572AspLys: 1.572 ± 0.03	5.458AspLeu: 5.458 ± 0.06	1.231AspMet: 1.231 ± 0.023	1.118AspAsn: 1.118 ± 0.026	2.897AspPro: 2.897 ± 0.042	1.699AspGln: 1.699 ± 0.029	3.274AspArg: 3.274 ± 0.04	2.126AspSer: 2.126 ± 0.037	2.607AspThr: 2.607 ± 0.05	4.015AspVal: 4.015 ± 0.049	0.946AspTrp: 0.946 ± 0.023	1.264AspTyr: 1.264 ± 0.024	0.002AspXaa: 0.002 ± 0.001
Glu	6.656GluAla: 6.656 ± 0.066	0.327GluCys: 0.327 ± 0.013	2.096GluAsp: 2.096 ± 0.033	2.189GluGlu: 2.189 ± 0.045	1.577GluPhe: 1.577 ± 0.029	3.432GluGly: 3.432 ± 0.05	1.301GluHis: 1.301 ± 0.027	2.313GluIle: 2.313 ± 0.036	1.434GluLys: 1.434 ± 0.032	5.536GluLeu: 5.536 ± 0.053	1.13GluMet: 1.13 ± 0.025	1.048GluAsn: 1.048 ± 0.025	2.475GluPro: 2.475 ± 0.041	2.507GluGln: 2.507 ± 0.038	4.598GluArg: 4.598 ± 0.066	2.149GluSer: 2.149 ± 0.034	2.262GluThr: 2.262 ± 0.039	3.677GluVal: 3.677 ± 0.046	0.635GluTrp: 0.635 ± 0.02	0.859GluTyr: 0.859 ± 0.022	0.001GluXaa: 0.001 ± 0.001
Phe	4.367PheAla: 4.367 ± 0.053	0.372PheCys: 0.372 ± 0.015	2.462PheAsp: 2.462 ± 0.04	1.918PheGlu: 1.918 ± 0.031	1.243PhePhe: 1.243 ± 0.028	3.358PheGly: 3.358 ± 0.042	0.706PheHis: 0.706 ± 0.018	1.337PheIle: 1.337 ± 0.027	1.076PheLys: 1.076 ± 0.025	2.735PheLeu: 2.735 ± 0.04	0.769PheMet: 0.769 ± 0.021	0.991PheAsn: 0.991 ± 0.022	1.425PhePro: 1.425 ± 0.031	1.049PheGln: 1.049 ± 0.023	1.761PheArg: 1.761 ± 0.034	1.952PheSer: 1.952 ± 0.033	1.857PheThr: 1.857 ± 0.033	2.748PheVal: 2.748 ± 0.041	0.49PheTrp: 0.49 ± 0.018	0.778PheTyr: 0.778 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	10.272GlyAla: 10.272 ± 0.136	0.918GlyCys: 0.918 ± 0.023	4.034GlyAsp: 4.034 ± 0.06	4.095GlyGlu: 4.095 ± 0.045	3.206GlyPhe: 3.206 ± 0.045	7.335GlyGly: 7.335 ± 0.104	2.007GlyHis: 2.007 ± 0.036	3.858GlyIle: 3.858 ± 0.048	2.938GlyLys: 2.938 ± 0.05	9.434GlyLeu: 9.434 ± 0.076	2.361GlyMet: 2.361 ± 0.038	2.077GlyAsn: 2.077 ± 0.046	3.468GlyPro: 3.468 ± 0.047	3.744GlyGln: 3.744 ± 0.044	5.882GlyArg: 5.882 ± 0.062	4.577GlySer: 4.577 ± 0.066	4.806GlyThr: 4.806 ± 0.066	6.685GlyVal: 6.685 ± 0.066	1.49GlyTrp: 1.49 ± 0.034	2.263GlyTyr: 2.263 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	3.33HisAla: 3.33 ± 0.051	0.27HisCys: 0.27 ± 0.013	1.267HisAsp: 1.267 ± 0.026	1.124HisGlu: 1.124 ± 0.025	0.8HisPhe: 0.8 ± 0.022	2.348HisGly: 2.348 ± 0.04	0.617HisHis: 0.617 ± 0.022	0.87HisIle: 0.87 ± 0.022	0.553HisLys: 0.553 ± 0.017	2.279HisLeu: 2.279 ± 0.036	0.485HisMet: 0.485 ± 0.015	0.484HisAsn: 0.484 ± 0.017	1.484HisPro: 1.484 ± 0.032	0.797HisGln: 0.797 ± 0.022	1.548HisArg: 1.548 ± 0.028	0.941HisSer: 0.941 ± 0.022	1.043HisThr: 1.043 ± 0.024	1.729HisVal: 1.729 ± 0.029	0.428HisTrp: 0.428 ± 0.015	0.598HisTyr: 0.598 ± 0.017	0.0HisXaa: 0.0 ± 0.0
Ile	6.498IleAla: 6.498 ± 0.059	0.376IleCys: 0.376 ± 0.014	2.825IleAsp: 2.825 ± 0.041	2.638IleGlu: 2.638 ± 0.042	1.092IlePhe: 1.092 ± 0.027	4.052IleGly: 4.052 ± 0.057	0.764IleHis: 0.764 ± 0.019	1.087IleIle: 1.087 ± 0.03	1.187IleLys: 1.187 ± 0.032	2.822IleLeu: 2.822 ± 0.045	0.65IleMet: 0.65 ± 0.021	1.096IleAsn: 1.096 ± 0.021	1.811IlePro: 1.811 ± 0.034	1.148IleGln: 1.148 ± 0.026	2.288IleArg: 2.288 ± 0.035	1.914IleSer: 1.914 ± 0.035	2.276IleThr: 2.276 ± 0.037	3.576IleVal: 3.576 ± 0.044	0.45IleTrp: 0.45 ± 0.016	0.801IleTyr: 0.801 ± 0.025	0.002IleXaa: 0.002 ± 0.001
Lys	3.611LysAla: 3.611 ± 0.043	0.124LysCys: 0.124 ± 0.008	1.432LysAsp: 1.432 ± 0.031	1.204LysGlu: 1.204 ± 0.027	0.813LysPhe: 0.813 ± 0.022	2.099LysGly: 2.099 ± 0.037	0.523LysHis: 0.523 ± 0.016	1.233LysIle: 1.233 ± 0.031	1.104LysLys: 1.104 ± 0.034	3.08LysLeu: 3.08 ± 0.044	0.677LysMet: 0.677 ± 0.02	0.69LysAsn: 0.69 ± 0.021	1.918LysPro: 1.918 ± 0.036	0.944LysGln: 0.944 ± 0.019	1.873LysArg: 1.873 ± 0.032	1.5LysSer: 1.5 ± 0.034	1.725LysThr: 1.725 ± 0.034	2.145LysVal: 2.145 ± 0.037	0.299LysTrp: 0.299 ± 0.014	0.524LysTyr: 0.524 ± 0.018	0.001LysXaa: 0.001 ± 0.0
Leu	17.074LeuAla: 17.074 ± 0.134	1.039LeuCys: 1.039 ± 0.024	6.031LeuAsp: 6.031 ± 0.053	4.673LeuGlu: 4.673 ± 0.055	3.291LeuPhe: 3.291 ± 0.05	8.895LeuGly: 8.895 ± 0.075	2.579LeuHis: 2.579 ± 0.042	3.665LeuIle: 3.665 ± 0.056	2.973LeuLys: 2.973 ± 0.049	11.978LeuLeu: 11.978 ± 0.12	2.413LeuMet: 2.413 ± 0.043	2.406LeuAsn: 2.406 ± 0.034	6.427LeuPro: 6.427 ± 0.076	5.097LeuGln: 5.097 ± 0.053	8.403LeuArg: 8.403 ± 0.075	5.615LeuSer: 5.615 ± 0.061	5.12LeuThr: 5.12 ± 0.061	8.663LeuVal: 8.663 ± 0.073	1.383LeuTrp: 1.383 ± 0.036	2.013LeuTyr: 2.013 ± 0.035	0.004LeuXaa: 0.004 ± 0.001
Met	3.407MetAla: 3.407 ± 0.041	0.175MetCys: 0.175 ± 0.01	1.226MetAsp: 1.226 ± 0.027	0.954MetGlu: 0.954 ± 0.023	0.691MetPhe: 0.691 ± 0.021	1.962MetGly: 1.962 ± 0.032	0.563MetHis: 0.563 ± 0.018	0.715MetIle: 0.715 ± 0.021	0.772MetLys: 0.772 ± 0.019	2.747MetLeu: 2.747 ± 0.046	0.484MetMet: 0.484 ± 0.016	0.742MetAsn: 0.742 ± 0.022	1.607MetPro: 1.607 ± 0.03	1.188MetGln: 1.188 ± 0.03	1.828MetArg: 1.828 ± 0.03	1.353MetSer: 1.353 ± 0.032	1.469MetThr: 1.469 ± 0.025	1.859MetVal: 1.859 ± 0.037	0.2MetTrp: 0.2 ± 0.011	0.335MetTyr: 0.335 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.225AsnAla: 3.225 ± 0.055	0.217AsnCys: 0.217 ± 0.01	1.171AsnAsp: 1.171 ± 0.03	1.04AsnGlu: 1.04 ± 0.023	0.839AsnPhe: 0.839 ± 0.021	2.007AsnGly: 2.007 ± 0.034	0.462AsnHis: 0.462 ± 0.016	1.051AsnIle: 1.051 ± 0.025	0.695AsnLys: 0.695 ± 0.019	2.405AsnLeu: 2.405 ± 0.039	0.521AsnMet: 0.521 ± 0.015	0.624AsnAsn: 0.624 ± 0.019	1.641AsnPro: 1.641 ± 0.03	0.783AsnGln: 0.783 ± 0.019	1.499AsnArg: 1.499 ± 0.032	0.972AsnSer: 0.972 ± 0.024	1.297AsnThr: 1.297 ± 0.031	1.759AsnVal: 1.759 ± 0.033	0.364AsnTrp: 0.364 ± 0.014	0.587AsnTyr: 0.587 ± 0.019	0.001AsnXaa: 0.001 ± 0.001
Pro	8.111ProAla: 8.111 ± 0.08	0.352ProCys: 0.352 ± 0.014	3.244ProAsp: 3.244 ± 0.042	3.002ProGlu: 3.002 ± 0.044	1.783ProPhe: 1.783 ± 0.033	5.045ProGly: 5.045 ± 0.054	1.236ProHis: 1.236 ± 0.026	1.866ProIle: 1.866 ± 0.035	1.263ProLys: 1.263 ± 0.031	5.524ProLeu: 5.524 ± 0.059	1.398ProMet: 1.398 ± 0.028	1.156ProAsn: 1.156 ± 0.026	2.836ProPro: 2.836 ± 0.044	2.332ProGln: 2.332 ± 0.039	3.26ProArg: 3.26 ± 0.053	2.646ProSer: 2.646 ± 0.039	2.751ProThr: 2.751 ± 0.032	4.422ProVal: 4.422 ± 0.053	0.889ProTrp: 0.889 ± 0.025	1.184ProTyr: 1.184 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	6.948GlnAla: 6.948 ± 0.078	0.311GlnCys: 0.311 ± 0.012	1.79GlnAsp: 1.79 ± 0.032	1.486GlnGlu: 1.486 ± 0.026	1.246GlnPhe: 1.246 ± 0.023	3.502GlnGly: 3.502 ± 0.042	1.069GlnHis: 1.069 ± 0.023	1.731GlnIle: 1.731 ± 0.029	0.878GlnLys: 0.878 ± 0.02	4.598GlnLeu: 4.598 ± 0.051	0.984GlnMet: 0.984 ± 0.022	0.773GlnAsn: 0.773 ± 0.021	2.558GlnPro: 2.558 ± 0.041	2.186GlnGln: 2.186 ± 0.042	3.916GlnArg: 3.916 ± 0.049	2.016GlnSer: 2.016 ± 0.032	1.918GlnThr: 1.918 ± 0.036	3.29GlnVal: 3.29 ± 0.041	0.637GlnTrp: 0.637 ± 0.019	0.742GlnTyr: 0.742 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.0ArgAla: 9.0 ± 0.078	0.626ArgCys: 0.626 ± 0.02	3.686ArgAsp: 3.686 ± 0.046	3.934ArgGlu: 3.934 ± 0.051	2.765ArgPhe: 2.765 ± 0.042	4.902ArgGly: 4.902 ± 0.054	1.997ArgHis: 1.997 ± 0.034	3.392ArgIle: 3.392 ± 0.049	1.914ArgLys: 1.914 ± 0.032	8.259ArgLeu: 8.259 ± 0.088	2.041ArgMet: 2.041 ± 0.031	1.703ArgAsn: 1.703 ± 0.032	3.465ArgPro: 3.465 ± 0.044	3.226ArgGln: 3.226 ± 0.05	5.382ArgArg: 5.382 ± 0.062	3.581ArgSer: 3.581 ± 0.045	3.443ArgThr: 3.443 ± 0.041	5.306ArgVal: 5.306 ± 0.054	1.3ArgTrp: 1.3 ± 0.031	1.795ArgTyr: 1.795 ± 0.031	0.002ArgXaa: 0.002 ± 0.001
Ser	6.546SerAla: 6.546 ± 0.067	0.416SerCys: 0.416 ± 0.015	2.428SerAsp: 2.428 ± 0.038	2.149SerGlu: 2.149 ± 0.033	1.999SerPhe: 1.999 ± 0.032	4.927SerGly: 4.927 ± 0.063	1.073SerHis: 1.073 ± 0.022	2.099SerIle: 2.099 ± 0.036	1.287SerLys: 1.287 ± 0.03	5.353SerLeu: 5.353 ± 0.062	1.267SerMet: 1.267 ± 0.027	1.267SerAsn: 1.267 ± 0.025	2.711SerPro: 2.711 ± 0.043	1.744SerGln: 1.744 ± 0.034	3.29SerArg: 3.29 ± 0.041	2.661SerSer: 2.661 ± 0.046	2.853SerThr: 2.853 ± 0.045	3.838SerVal: 3.838 ± 0.059	0.718SerTrp: 0.718 ± 0.021	1.164SerTyr: 1.164 ± 0.029	0.001SerXaa: 0.001 ± 0.0
Thr	6.589ThrAla: 6.589 ± 0.079	0.348ThrCys: 0.348 ± 0.014	2.427ThrAsp: 2.427 ± 0.042	2.217ThrGlu: 2.217 ± 0.034	1.512ThrPhe: 1.512 ± 0.026	4.795ThrGly: 4.795 ± 0.065	1.211ThrHis: 1.211 ± 0.029	1.938ThrIle: 1.938 ± 0.033	1.092ThrLys: 1.092 ± 0.026	6.407ThrLeu: 6.407 ± 0.075	1.101ThrMet: 1.101 ± 0.026	1.092ThrAsn: 1.092 ± 0.023	3.689ThrPro: 3.689 ± 0.048	2.018ThrGln: 2.018 ± 0.035	3.492ThrArg: 3.492 ± 0.05	2.527ThrSer: 2.527 ± 0.038	2.903ThrThr: 2.903 ± 0.045	4.475ThrVal: 4.475 ± 0.072	0.656ThrTrp: 0.656 ± 0.018	0.965ThrTyr: 0.965 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.448ValAla: 10.448 ± 0.087	0.737ValCys: 0.737 ± 0.02	4.331ValAsp: 4.331 ± 0.05	3.868ValGlu: 3.868 ± 0.046	2.685ValPhe: 2.685 ± 0.037	5.892ValGly: 5.892 ± 0.067	1.742ValHis: 1.742 ± 0.033	2.942ValIle: 2.942 ± 0.044	2.056ValLys: 2.056 ± 0.042	9.114ValLeu: 9.114 ± 0.073	1.881ValMet: 1.881 ± 0.036	1.964ValAsn: 1.964 ± 0.035	4.372ValPro: 4.372 ± 0.049	3.385ValGln: 3.385 ± 0.044	5.588ValArg: 5.588 ± 0.054	3.755ValSer: 3.755 ± 0.055	3.927ValThr: 3.927 ± 0.069	6.772ValVal: 6.772 ± 0.074	0.991ValTrp: 0.991 ± 0.022	1.619ValTyr: 1.619 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.583TrpAla: 1.583 ± 0.034	0.195TrpCys: 0.195 ± 0.012	0.65TrpAsp: 0.65 ± 0.02	0.496TrpGlu: 0.496 ± 0.016	0.556TrpPhe: 0.556 ± 0.015	1.017TrpGly: 1.017 ± 0.026	0.378TrpHis: 0.378 ± 0.015	0.591TrpIle: 0.591 ± 0.019	0.377TrpLys: 0.377 ± 0.012	2.098TrpLeu: 2.098 ± 0.044	0.429TrpMet: 0.429 ± 0.016	0.427TrpAsn: 0.427 ± 0.015	0.805TrpPro: 0.805 ± 0.024	0.784TrpGln: 0.784 ± 0.024	1.253TrpArg: 1.253 ± 0.03	0.811TrpSer: 0.811 ± 0.021	0.778TrpThr: 0.778 ± 0.022	1.0TrpVal: 1.0 ± 0.025	0.305TrpTrp: 0.305 ± 0.014	0.288TrpTyr: 0.288 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.779TyrAla: 2.779 ± 0.042	0.225TyrCys: 0.225 ± 0.011	1.185TyrAsp: 1.185 ± 0.025	1.05TyrGlu: 1.05 ± 0.025	0.853TyrPhe: 0.853 ± 0.026	1.963TyrGly: 1.963 ± 0.031	0.409TyrHis: 0.409 ± 0.014	0.693TyrIle: 0.693 ± 0.021	0.631TyrLys: 0.631 ± 0.018	2.199TyrLeu: 2.199 ± 0.038	0.398TyrMet: 0.398 ± 0.015	0.528TyrAsn: 0.528 ± 0.019	1.017TyrPro: 1.017 ± 0.024	0.848TyrGln: 0.848 ± 0.023	1.6TyrArg: 1.6 ± 0.03	1.035TyrSer: 1.035 ± 0.026	1.155TyrThr: 1.155 ± 0.026	1.571TyrVal: 1.571 ± 0.028	0.358TyrTrp: 0.358 ± 0.014	0.526TyrTyr: 0.526 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.002XaaGln: 0.002 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 5708 proteins (1914030 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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