Enorma massiliensis
Average proteome isoelectric point is 5.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1937 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y3U349|A0A1Y3U349_9ACTN Uncharacterized protein OS=Enorma massiliensis OX=1472761 GN=B5G21_07125 PE=4 SV=1
MM1 pKa = 7.34 FKK3 pKa = 10.04 WKK5 pKa = 10.31 APRR8 pKa = 11.84 VRR10 pKa = 11.84 SGRR13 pKa = 11.84 RR14 pKa = 11.84 LRR16 pKa = 11.84 RR17 pKa = 11.84 SARR20 pKa = 11.84 SHH22 pKa = 5.69 RR23 pKa = 11.84 VVAAVMAALLVWGQVPTYY41 pKa = 9.52 AWAGAATAEE50 pKa = 4.54 SAASAQALKK59 pKa = 10.8 SAIPQSRR66 pKa = 11.84 TSPSGNTSATSADD79 pKa = 3.54 STEE82 pKa = 4.15 ATGQEE87 pKa = 4.26 PASSSADD94 pKa = 3.4 TSKK97 pKa = 11.53 DD98 pKa = 3.2 DD99 pKa = 4.09 AASQGEE105 pKa = 4.28 QAAQGAGSTTSNTAAEE121 pKa = 4.44 SSGSEE126 pKa = 3.81 ASSTGSAASSEE137 pKa = 4.63 SPDD140 pKa = 3.21 GAAPSSADD148 pKa = 3.05 ASASNTATARR158 pKa = 11.84 TSSTAASANASDD170 pKa = 4.51 VSTQALTSNAKK181 pKa = 10.15 VLIQDD186 pKa = 3.96 SASEE190 pKa = 4.2 TSSYY194 pKa = 9.54 SQAYY198 pKa = 9.12 GSLSAGTVLWANLYY212 pKa = 10.36 DD213 pKa = 4.96 GSSSWSLSRR222 pKa = 11.84 VDD224 pKa = 6.15 AEE226 pKa = 5.97 DD227 pKa = 2.8 GWTYY231 pKa = 9.7 QWYY234 pKa = 9.17 AASEE238 pKa = 4.19 KK239 pKa = 10.99 SSDD242 pKa = 3.42 KK243 pKa = 10.92 SDD245 pKa = 3.47 YY246 pKa = 11.49 APIEE250 pKa = 4.45 DD251 pKa = 4.03 ATGQSLTVTEE261 pKa = 5.02 DD262 pKa = 3.16 LAGSYY267 pKa = 10.14 IIVCVTVDD275 pKa = 5.31 GIDD278 pKa = 2.74 RR279 pKa = 11.84 WAPLTSYY286 pKa = 11.51 GDD288 pKa = 4.07 TINQNYY294 pKa = 9.8 LPGPVLGQGQAEE306 pKa = 4.83 LYY308 pKa = 10.7 SVTLSNSSPSAGEE321 pKa = 3.99 TLTATANVSYY331 pKa = 8.11 NTPAGDD337 pKa = 4.02 DD338 pKa = 3.79 VNVTYY343 pKa = 9.04 TWQQGDD349 pKa = 3.28 SRR351 pKa = 11.84 YY352 pKa = 9.01 GTFEE356 pKa = 5.05 DD357 pKa = 5.1 IEE359 pKa = 4.57 GAGNGPTFTLTEE371 pKa = 4.16 AQQGKK376 pKa = 6.51 YY377 pKa = 8.23 VRR379 pKa = 11.84 VIASAGVNEE388 pKa = 4.34 EE389 pKa = 4.59 DD390 pKa = 3.69 ATTSDD395 pKa = 3.1 TVMAEE400 pKa = 4.22 GAVKK404 pKa = 10.43 LAGVEE409 pKa = 4.03 LATPGSLEE417 pKa = 3.97 MPVTLEE423 pKa = 3.66 AKK425 pKa = 10.15 AYY427 pKa = 7.7 TGSSYY432 pKa = 11.42 SPTYY436 pKa = 7.97 VTEE439 pKa = 4.17 GVTYY443 pKa = 8.2 TWMYY447 pKa = 10.99 AKK449 pKa = 9.6 TSSPSYY455 pKa = 7.98 STTWTVIEE463 pKa = 5.05 CEE465 pKa = 4.14 TGPTLTVDD473 pKa = 2.95 SDD475 pKa = 4.07 EE476 pKa = 4.44 YY477 pKa = 11.23 AGCCFRR483 pKa = 11.84 VQASAGANDD492 pKa = 3.83 VEE494 pKa = 4.29 VSYY497 pKa = 11.38 YY498 pKa = 10.78 NAIGPVKK505 pKa = 10.76 LEE507 pKa = 4.08 GQVDD511 pKa = 4.1 IYY513 pKa = 10.8 TAFLAGSADD522 pKa = 3.7 ATSGSYY528 pKa = 10.94 VFTSEE533 pKa = 3.53 DD534 pKa = 3.75 TAWAFARR541 pKa = 11.84 EE542 pKa = 4.31 QGGSVPIDD550 pKa = 3.58 SSKK553 pKa = 9.47 VTYY556 pKa = 8.66 QWQMSTTSRR565 pKa = 11.84 TSGFEE570 pKa = 4.74 DD571 pKa = 3.15 IDD573 pKa = 3.99 GATGQSLEE581 pKa = 3.94 LAKK584 pKa = 11.15 YY585 pKa = 7.42 EE586 pKa = 4.13 GCYY589 pKa = 9.04 VRR591 pKa = 11.84 CVITANVGGSTYY603 pKa = 10.55 ATTATMKK610 pKa = 10.07 IAAPGSVNVTSVSLDD625 pKa = 3.01 ASGKK629 pKa = 9.1 VNVGTTLTATASAASDD645 pKa = 3.72 DD646 pKa = 4.25 VTDD649 pKa = 4.09 DD650 pKa = 3.75 THH652 pKa = 7.26 VAWAWYY658 pKa = 10.08 YY659 pKa = 11.25 GDD661 pKa = 4.67 SSSATDD667 pKa = 3.4 TRR669 pKa = 11.84 IEE671 pKa = 4.21 GATGNTLEE679 pKa = 4.44 LTQEE683 pKa = 4.17 LVDD686 pKa = 4.95 EE687 pKa = 4.41 YY688 pKa = 11.37 DD689 pKa = 3.61 LLGKK693 pKa = 10.16 YY694 pKa = 9.61 VEE696 pKa = 4.41 ARR698 pKa = 11.84 ADD700 pKa = 3.47 GGFGEE705 pKa = 4.98 EE706 pKa = 4.74 DD707 pKa = 3.19 SSAAGPVIVPGAVEE721 pKa = 4.42 LYY723 pKa = 9.42 RR724 pKa = 11.84 VEE726 pKa = 4.13 VTGDD730 pKa = 3.13 ARR732 pKa = 11.84 IGSTLTATAYY742 pKa = 9.65 KK743 pKa = 10.18 NSYY746 pKa = 8.07 STVVSDD752 pKa = 3.88 TDD754 pKa = 3.19 AVTYY758 pKa = 8.65 QWQYY762 pKa = 9.82 ATTNTTSDD770 pKa = 3.88 AEE772 pKa = 4.29 FTDD775 pKa = 3.52 IPGATDD781 pKa = 3.15 RR782 pKa = 11.84 TYY784 pKa = 10.52 TIPEE788 pKa = 4.44 TIDD791 pKa = 3.29 GKK793 pKa = 10.35 DD794 pKa = 3.09 AHH796 pKa = 6.55 GLYY799 pKa = 10.63 LRR801 pKa = 11.84 VQAVSDD807 pKa = 3.94 GSVVSTYY814 pKa = 9.7 QRR816 pKa = 11.84 GRR818 pKa = 11.84 YY819 pKa = 8.8 SYY821 pKa = 11.01 SGSTYY826 pKa = 10.09 VDD828 pKa = 3.06 PAGPVMLQGQYY839 pKa = 9.41 TLSSVEE845 pKa = 4.5 LSSSGQGAQAGATITPAAQVRR866 pKa = 11.84 SGYY869 pKa = 10.6 YY870 pKa = 10.37 DD871 pKa = 3.13 VDD873 pKa = 3.81 APTDD877 pKa = 3.55 AQLTYY882 pKa = 8.48 TWYY885 pKa = 9.61 IARR888 pKa = 11.84 AGGSFEE894 pKa = 4.23 EE895 pKa = 5.29 LGDD898 pKa = 4.22 GYY900 pKa = 11.42 DD901 pKa = 3.48 PATGVLALTDD911 pKa = 3.87 DD912 pKa = 3.96 MVGATLKK919 pKa = 10.76 VSASALDD926 pKa = 3.57 NTVTSDD932 pKa = 3.38 EE933 pKa = 4.52 FVVVAAGTYY942 pKa = 10.37 DD943 pKa = 3.88 LARR946 pKa = 11.84 VNVSPSFTSSSTTLFTDD963 pKa = 3.79 DD964 pKa = 4.4 AVSASVWANGVDD976 pKa = 3.97 GYY978 pKa = 7.09 TTSVTGEE985 pKa = 3.9 VTIRR989 pKa = 11.84 WYY991 pKa = 10.95 VDD993 pKa = 3.17 EE994 pKa = 5.76 ASDD997 pKa = 5.09 GSFDD1001 pKa = 3.54 TCIGEE1006 pKa = 4.34 GTEE1009 pKa = 3.98 VTLPAEE1015 pKa = 4.43 AAGCYY1020 pKa = 9.66 LKK1022 pKa = 11.03 VVATSGASTVEE1033 pKa = 3.98 AVSANPVAGTDD1044 pKa = 3.49 SLAGIVAKK1052 pKa = 10.6 LDD1054 pKa = 3.72 AEE1056 pKa = 4.37 DD1057 pKa = 3.69 WRR1059 pKa = 11.84 PEE1061 pKa = 3.85 PVYY1064 pKa = 11.22 GEE1066 pKa = 4.19 DD1067 pKa = 3.92 ANVNDD1072 pKa = 3.69 VLQAKK1077 pKa = 8.69 LAEE1080 pKa = 4.25 MGVDD1084 pKa = 3.46 DD1085 pKa = 4.46 VDD1087 pKa = 3.78 VRR1089 pKa = 11.84 VTGVAFVNQNEE1100 pKa = 4.24 QASLGVSTANDD1111 pKa = 3.48 ATNGDD1116 pKa = 3.25 ITFFFIDD1123 pKa = 3.84 PDD1125 pKa = 4.05 VVTGFANYY1133 pKa = 7.39 TQWRR1137 pKa = 11.84 QLRR1140 pKa = 11.84 LTFEE1144 pKa = 4.63 LSRR1147 pKa = 11.84 DD1148 pKa = 3.73 GEE1150 pKa = 4.55 TATYY1154 pKa = 10.55 APGRR1158 pKa = 11.84 SVIIPWDD1165 pKa = 3.45 EE1166 pKa = 3.84 ARR1168 pKa = 11.84 VGEE1171 pKa = 4.3 LLEE1174 pKa = 4.57 EE1175 pKa = 4.32 KK1176 pKa = 10.6 AADD1179 pKa = 3.82 LAIGFASGDD1188 pKa = 3.37 TSNAVTGNLTLPYY1201 pKa = 9.66 KK1202 pKa = 10.78 LSDD1205 pKa = 3.35 GSGDD1209 pKa = 3.41 TRR1211 pKa = 11.84 SWSSVSWSSDD1221 pKa = 2.71 FDD1223 pKa = 3.59 AVRR1226 pKa = 11.84 ISGYY1230 pKa = 10.11 GWSDD1234 pKa = 3.26 YY1235 pKa = 10.73 TGTVTRR1241 pKa = 11.84 TAADD1245 pKa = 3.34 QEE1247 pKa = 4.62 VALTATIGVVASDD1260 pKa = 4.44 GPSTTIEE1267 pKa = 3.82 KK1268 pKa = 9.52 TFTVTVLGDD1277 pKa = 3.54 PALVEE1282 pKa = 4.33 AEE1284 pKa = 3.99 QAALQEE1290 pKa = 4.39 KK1291 pKa = 10.16 VDD1293 pKa = 3.74 AAFTADD1299 pKa = 3.54 ALTYY1303 pKa = 10.21 SADD1306 pKa = 3.53 GAAVDD1311 pKa = 3.96 PAAVVGDD1318 pKa = 3.98 LQLPRR1323 pKa = 11.84 PSAIGIDD1330 pKa = 3.6 GADD1333 pKa = 3.44 YY1334 pKa = 9.69 TITYY1338 pKa = 8.81 AASTDD1343 pKa = 3.84 AIEE1346 pKa = 4.19 LNGYY1350 pKa = 8.83 RR1351 pKa = 11.84 GNVYY1355 pKa = 10.3 QPLPDD1360 pKa = 4.43 ADD1362 pKa = 3.95 GAPVEE1367 pKa = 4.33 LTLTVTSKK1375 pKa = 11.37 ANPEE1379 pKa = 3.53 ITASKK1384 pKa = 9.23 TIQLTVAPLEE1394 pKa = 4.66 AGDD1397 pKa = 3.83 ITSEE1401 pKa = 4.07 LALMEE1406 pKa = 4.35 AAKK1409 pKa = 10.48 AGYY1412 pKa = 8.79 GQALLGEE1419 pKa = 4.56 NASADD1424 pKa = 3.42 AVTTNLHH1431 pKa = 6.39 AFQKK1435 pKa = 10.81 AYY1437 pKa = 10.83 LDD1439 pKa = 4.06 EE1440 pKa = 4.92 NSVLTWAYY1448 pKa = 10.43 DD1449 pKa = 3.37 SAAAGVAGDD1458 pKa = 4.84 GIVATDD1464 pKa = 3.89 LPGYY1468 pKa = 10.6 DD1469 pKa = 3.88 PMGSAGWRR1477 pKa = 11.84 LFRR1480 pKa = 11.84 SSNAGVVAHH1489 pKa = 6.83 EE1490 pKa = 4.63 SLVVTQPTYY1499 pKa = 9.16 NTEE1502 pKa = 3.9 VTITSVLTSEE1512 pKa = 4.37 KK1513 pKa = 8.5 YY1514 pKa = 9.54 ARR1516 pKa = 11.84 YY1517 pKa = 9.9 AEE1519 pKa = 4.76 RR1520 pKa = 11.84 YY1521 pKa = 9.91 ADD1523 pKa = 3.97 DD1524 pKa = 4.28 EE1525 pKa = 4.59 TWGPAFSLLANQEE1538 pKa = 3.74 VSATFTVLGTSGEE1551 pKa = 4.24 QNPSVTATVRR1561 pKa = 11.84 IVGVDD1566 pKa = 3.17 ASGVDD1571 pKa = 3.8 EE1572 pKa = 4.58 AWVVTTAYY1580 pKa = 9.96 EE1581 pKa = 4.06 LTEE1584 pKa = 4.39 GSTAADD1590 pKa = 3.55 LTEE1593 pKa = 4.69 AALADD1598 pKa = 3.92 AGLTADD1604 pKa = 4.05 YY1605 pKa = 8.39 GTGSRR1610 pKa = 11.84 GWYY1613 pKa = 9.73 LNSITSADD1621 pKa = 3.67 GRR1623 pKa = 11.84 VLEE1626 pKa = 4.26 YY1627 pKa = 11.19 DD1628 pKa = 3.82 EE1629 pKa = 5.61 DD1630 pKa = 3.94 TGKK1633 pKa = 10.58 YY1634 pKa = 7.01 WQLFVNGSASDD1645 pKa = 3.47 AGAGEE1650 pKa = 4.42 VTLQAGDD1657 pKa = 4.16 EE1658 pKa = 4.24 ITWYY1662 pKa = 9.96 YY1663 pKa = 9.51 SAWGASLDD1671 pKa = 4.76 DD1672 pKa = 3.89 IGQARR1677 pKa = 11.84 ISVTASVIGLDD1688 pKa = 4.41 ADD1690 pKa = 4.17 GEE1692 pKa = 4.38 DD1693 pKa = 4.31 ASWVPTTRR1701 pKa = 11.84 VQLAEE1706 pKa = 4.2 GSTAAEE1712 pKa = 4.11 LTEE1715 pKa = 4.23 EE1716 pKa = 4.6 LLTSAGLEE1724 pKa = 4.14 HH1725 pKa = 7.29 EE1726 pKa = 4.37 ATGVGTGGYY1735 pKa = 8.85 YY1736 pKa = 10.78 LSTITSTDD1744 pKa = 2.67 GRR1746 pKa = 11.84 TLGWDD1751 pKa = 3.75 EE1752 pKa = 4.51 DD1753 pKa = 4.34 TGCYY1757 pKa = 7.66 WQLFVNGSASDD1768 pKa = 3.48 AGAGQVTLKK1777 pKa = 10.64 PGDD1780 pKa = 3.87 EE1781 pKa = 4.14 IAWYY1785 pKa = 10.39 YY1786 pKa = 9.54 SAQGAEE1792 pKa = 4.4 LPTNDD1797 pKa = 4.76 VEE1799 pKa = 4.55 VDD1801 pKa = 3.38 PDD1803 pKa = 3.87 APRR1806 pKa = 11.84 PDD1808 pKa = 4.49 YY1809 pKa = 11.07 DD1810 pKa = 4.31 AAWPTYY1816 pKa = 10.82 APGAVTDD1823 pKa = 3.77 VATPTEE1829 pKa = 4.4 GGEE1832 pKa = 4.24 LAWSVDD1838 pKa = 3.7 LGTDD1842 pKa = 3.4 MASSVYY1848 pKa = 10.6 ASDD1851 pKa = 4.77 PIVVNGNLYY1860 pKa = 10.52 LAVGDD1865 pKa = 3.99 EE1866 pKa = 4.55 LRR1868 pKa = 11.84 VLDD1871 pKa = 4.31 AATGKK1876 pKa = 10.02 ILATATLATSVDD1888 pKa = 3.89 SACRR1892 pKa = 11.84 LAYY1895 pKa = 10.19 ADD1897 pKa = 3.83 GLVVVPLHH1905 pKa = 6.34 GGRR1908 pKa = 11.84 LQALTADD1915 pKa = 3.8 TLTTVSLTDD1924 pKa = 3.46 EE1925 pKa = 4.4 LAAGQQALSTLTISDD1940 pKa = 2.81 GRR1942 pKa = 11.84 AYY1944 pKa = 10.64 FGTTSWTGEE1953 pKa = 4.16 TGTLFCVEE1961 pKa = 4.73 LATGAVVWSTSGDD1974 pKa = 3.42 GDD1976 pKa = 4.5 AGYY1979 pKa = 9.3 YY1980 pKa = 8.36 WCGAVLAGDD1989 pKa = 3.57 WLVAVDD1995 pKa = 3.7 GAGVVRR2001 pKa = 11.84 AYY2003 pKa = 10.67 DD2004 pKa = 3.54 PATGEE2009 pKa = 4.07 VAGTIDD2015 pKa = 4.03 LGANAHH2021 pKa = 5.62 VSVVADD2027 pKa = 3.93 GSDD2030 pKa = 3.49 LYY2032 pKa = 11.74 VVTNDD2037 pKa = 3.4 GTLHH2041 pKa = 6.63 RR2042 pKa = 11.84 LALAADD2048 pKa = 4.64 GSLTQTGSVRR2058 pKa = 11.84 FADD2061 pKa = 4.13 SSTSTPTIADD2071 pKa = 2.85 GRR2073 pKa = 11.84 AYY2075 pKa = 10.87 VGGANDD2081 pKa = 4.37 DD2082 pKa = 3.95 STGVLAVIDD2091 pKa = 4.34 LATMGVEE2098 pKa = 3.85 HH2099 pKa = 7.65 RR2100 pKa = 11.84 ITGFSNEE2107 pKa = 4.04 AKK2109 pKa = 10.67 LPGDD2113 pKa = 3.75 VKK2115 pKa = 10.88 SAPTVSARR2123 pKa = 11.84 DD2124 pKa = 3.62 GEE2126 pKa = 4.73 TYY2128 pKa = 10.92 VYY2130 pKa = 7.21 FTCNAVSGGAYY2141 pKa = 10.35 LYY2143 pKa = 10.84 HH2144 pKa = 7.84 LGDD2147 pKa = 3.36 AHH2149 pKa = 7.55 ASVLYY2154 pKa = 10.83 LPGDD2158 pKa = 4.31 DD2159 pKa = 4.19 QADD2162 pKa = 3.21 WCMASVVVGADD2173 pKa = 3.11 GALYY2177 pKa = 10.09 YY2178 pKa = 11.34 VNDD2181 pKa = 3.65 SGTLFKK2187 pKa = 11.26 LEE2189 pKa = 4.64 AGAALPDD2196 pKa = 3.98 PTPTDD2201 pKa = 4.33 DD2202 pKa = 5.02 GDD2204 pKa = 3.76 GTTGDD2209 pKa = 3.77 NTNGSTSNTGDD2220 pKa = 3.23 DD2221 pKa = 3.89 TGDD2224 pKa = 3.32 VDD2226 pKa = 5.96 SGDD2229 pKa = 4.25 DD2230 pKa = 3.79 LDD2232 pKa = 5.88 GGAHH2236 pKa = 6.66 RR2237 pKa = 11.84 PTGTVQPGRR2246 pKa = 11.84 QPIATDD2252 pKa = 3.43 DD2253 pKa = 3.96 AAVDD2257 pKa = 3.88 EE2258 pKa = 5.01 AADD2261 pKa = 3.87 DD2262 pKa = 4.3 ALTEE2266 pKa = 4.3 EE2267 pKa = 4.88 TSSSDD2272 pKa = 3.22 EE2273 pKa = 4.18 TALSAGARR2281 pKa = 11.84 VSSSADD2287 pKa = 2.85 AGAAEE2292 pKa = 4.39 QPRR2295 pKa = 11.84 SFRR2298 pKa = 11.84 WLPIAGIILGVCGLVVIIVYY2318 pKa = 8.14 VKK2320 pKa = 10.34 RR2321 pKa = 11.84 RR2322 pKa = 11.84 EE2323 pKa = 3.9 HH2324 pKa = 7.48 SEE2326 pKa = 3.73
Molecular weight: 240.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.704
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.012
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.554
Solomon 3.859
Lehninger 3.808
Nozaki 3.961
DTASelect 4.215
Thurlkill 3.681
EMBOSS 3.783
Sillero 3.986
Patrickios 1.087
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.885
Protein with the highest isoelectric point:
>tr|A0A1Y3U203|A0A1Y3U203_9ACTN Uncharacterized protein OS=Enorma massiliensis OX=1472761 GN=B5G21_10080 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 GGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.88 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1937
0
1937
655953
25
3004
338.6
36.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.872 ± 0.084
1.504 ± 0.023
6.23 ± 0.045
6.866 ± 0.054
3.584 ± 0.035
8.279 ± 0.049
1.994 ± 0.026
5.136 ± 0.045
3.24 ± 0.047
9.123 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.025
2.733 ± 0.039
4.374 ± 0.035
2.886 ± 0.028
6.559 ± 0.068
5.982 ± 0.05
5.365 ± 0.057
7.86 ± 0.043
1.029 ± 0.023
2.809 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here