Enorma massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Coriobacteriaceae; Enorma

Average proteome isoelectric point is 5.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1937 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y3U349|A0A1Y3U349_9ACTN Uncharacterized protein OS=Enorma massiliensis OX=1472761 GN=B5G21_07125 PE=4 SV=1
MM1 pKa = 7.34FKK3 pKa = 10.04WKK5 pKa = 10.31APRR8 pKa = 11.84VRR10 pKa = 11.84SGRR13 pKa = 11.84RR14 pKa = 11.84LRR16 pKa = 11.84RR17 pKa = 11.84SARR20 pKa = 11.84SHH22 pKa = 5.69RR23 pKa = 11.84VVAAVMAALLVWGQVPTYY41 pKa = 9.52AWAGAATAEE50 pKa = 4.54SAASAQALKK59 pKa = 10.8SAIPQSRR66 pKa = 11.84TSPSGNTSATSADD79 pKa = 3.54STEE82 pKa = 4.15ATGQEE87 pKa = 4.26PASSSADD94 pKa = 3.4TSKK97 pKa = 11.53DD98 pKa = 3.2DD99 pKa = 4.09AASQGEE105 pKa = 4.28QAAQGAGSTTSNTAAEE121 pKa = 4.44SSGSEE126 pKa = 3.81ASSTGSAASSEE137 pKa = 4.63SPDD140 pKa = 3.21GAAPSSADD148 pKa = 3.05ASASNTATARR158 pKa = 11.84TSSTAASANASDD170 pKa = 4.51VSTQALTSNAKK181 pKa = 10.15VLIQDD186 pKa = 3.96SASEE190 pKa = 4.2TSSYY194 pKa = 9.54SQAYY198 pKa = 9.12GSLSAGTVLWANLYY212 pKa = 10.36DD213 pKa = 4.96GSSSWSLSRR222 pKa = 11.84VDD224 pKa = 6.15AEE226 pKa = 5.97DD227 pKa = 2.8GWTYY231 pKa = 9.7QWYY234 pKa = 9.17AASEE238 pKa = 4.19KK239 pKa = 10.99SSDD242 pKa = 3.42KK243 pKa = 10.92SDD245 pKa = 3.47YY246 pKa = 11.49APIEE250 pKa = 4.45DD251 pKa = 4.03ATGQSLTVTEE261 pKa = 5.02DD262 pKa = 3.16LAGSYY267 pKa = 10.14IIVCVTVDD275 pKa = 5.31GIDD278 pKa = 2.74RR279 pKa = 11.84WAPLTSYY286 pKa = 11.51GDD288 pKa = 4.07TINQNYY294 pKa = 9.8LPGPVLGQGQAEE306 pKa = 4.83LYY308 pKa = 10.7SVTLSNSSPSAGEE321 pKa = 3.99TLTATANVSYY331 pKa = 8.11NTPAGDD337 pKa = 4.02DD338 pKa = 3.79VNVTYY343 pKa = 9.04TWQQGDD349 pKa = 3.28SRR351 pKa = 11.84YY352 pKa = 9.01GTFEE356 pKa = 5.05DD357 pKa = 5.1IEE359 pKa = 4.57GAGNGPTFTLTEE371 pKa = 4.16AQQGKK376 pKa = 6.51YY377 pKa = 8.23VRR379 pKa = 11.84VIASAGVNEE388 pKa = 4.34EE389 pKa = 4.59DD390 pKa = 3.69ATTSDD395 pKa = 3.1TVMAEE400 pKa = 4.22GAVKK404 pKa = 10.43LAGVEE409 pKa = 4.03LATPGSLEE417 pKa = 3.97MPVTLEE423 pKa = 3.66AKK425 pKa = 10.15AYY427 pKa = 7.7TGSSYY432 pKa = 11.42SPTYY436 pKa = 7.97VTEE439 pKa = 4.17GVTYY443 pKa = 8.2TWMYY447 pKa = 10.99AKK449 pKa = 9.6TSSPSYY455 pKa = 7.98STTWTVIEE463 pKa = 5.05CEE465 pKa = 4.14TGPTLTVDD473 pKa = 2.95SDD475 pKa = 4.07EE476 pKa = 4.44YY477 pKa = 11.23AGCCFRR483 pKa = 11.84VQASAGANDD492 pKa = 3.83VEE494 pKa = 4.29VSYY497 pKa = 11.38YY498 pKa = 10.78NAIGPVKK505 pKa = 10.76LEE507 pKa = 4.08GQVDD511 pKa = 4.1IYY513 pKa = 10.8TAFLAGSADD522 pKa = 3.7ATSGSYY528 pKa = 10.94VFTSEE533 pKa = 3.53DD534 pKa = 3.75TAWAFARR541 pKa = 11.84EE542 pKa = 4.31QGGSVPIDD550 pKa = 3.58SSKK553 pKa = 9.47VTYY556 pKa = 8.66QWQMSTTSRR565 pKa = 11.84TSGFEE570 pKa = 4.74DD571 pKa = 3.15IDD573 pKa = 3.99GATGQSLEE581 pKa = 3.94LAKK584 pKa = 11.15YY585 pKa = 7.42EE586 pKa = 4.13GCYY589 pKa = 9.04VRR591 pKa = 11.84CVITANVGGSTYY603 pKa = 10.55ATTATMKK610 pKa = 10.07IAAPGSVNVTSVSLDD625 pKa = 3.01ASGKK629 pKa = 9.1VNVGTTLTATASAASDD645 pKa = 3.72DD646 pKa = 4.25VTDD649 pKa = 4.09DD650 pKa = 3.75THH652 pKa = 7.26VAWAWYY658 pKa = 10.08YY659 pKa = 11.25GDD661 pKa = 4.67SSSATDD667 pKa = 3.4TRR669 pKa = 11.84IEE671 pKa = 4.21GATGNTLEE679 pKa = 4.44LTQEE683 pKa = 4.17LVDD686 pKa = 4.95EE687 pKa = 4.41YY688 pKa = 11.37DD689 pKa = 3.61LLGKK693 pKa = 10.16YY694 pKa = 9.61VEE696 pKa = 4.41ARR698 pKa = 11.84ADD700 pKa = 3.47GGFGEE705 pKa = 4.98EE706 pKa = 4.74DD707 pKa = 3.19SSAAGPVIVPGAVEE721 pKa = 4.42LYY723 pKa = 9.42RR724 pKa = 11.84VEE726 pKa = 4.13VTGDD730 pKa = 3.13ARR732 pKa = 11.84IGSTLTATAYY742 pKa = 9.65KK743 pKa = 10.18NSYY746 pKa = 8.07STVVSDD752 pKa = 3.88TDD754 pKa = 3.19AVTYY758 pKa = 8.65QWQYY762 pKa = 9.82ATTNTTSDD770 pKa = 3.88AEE772 pKa = 4.29FTDD775 pKa = 3.52IPGATDD781 pKa = 3.15RR782 pKa = 11.84TYY784 pKa = 10.52TIPEE788 pKa = 4.44TIDD791 pKa = 3.29GKK793 pKa = 10.35DD794 pKa = 3.09AHH796 pKa = 6.55GLYY799 pKa = 10.63LRR801 pKa = 11.84VQAVSDD807 pKa = 3.94GSVVSTYY814 pKa = 9.7QRR816 pKa = 11.84GRR818 pKa = 11.84YY819 pKa = 8.8SYY821 pKa = 11.01SGSTYY826 pKa = 10.09VDD828 pKa = 3.06PAGPVMLQGQYY839 pKa = 9.41TLSSVEE845 pKa = 4.5LSSSGQGAQAGATITPAAQVRR866 pKa = 11.84SGYY869 pKa = 10.6YY870 pKa = 10.37DD871 pKa = 3.13VDD873 pKa = 3.81APTDD877 pKa = 3.55AQLTYY882 pKa = 8.48TWYY885 pKa = 9.61IARR888 pKa = 11.84AGGSFEE894 pKa = 4.23EE895 pKa = 5.29LGDD898 pKa = 4.22GYY900 pKa = 11.42DD901 pKa = 3.48PATGVLALTDD911 pKa = 3.87DD912 pKa = 3.96MVGATLKK919 pKa = 10.76VSASALDD926 pKa = 3.57NTVTSDD932 pKa = 3.38EE933 pKa = 4.52FVVVAAGTYY942 pKa = 10.37DD943 pKa = 3.88LARR946 pKa = 11.84VNVSPSFTSSSTTLFTDD963 pKa = 3.79DD964 pKa = 4.4AVSASVWANGVDD976 pKa = 3.97GYY978 pKa = 7.09TTSVTGEE985 pKa = 3.9VTIRR989 pKa = 11.84WYY991 pKa = 10.95VDD993 pKa = 3.17EE994 pKa = 5.76ASDD997 pKa = 5.09GSFDD1001 pKa = 3.54TCIGEE1006 pKa = 4.34GTEE1009 pKa = 3.98VTLPAEE1015 pKa = 4.43AAGCYY1020 pKa = 9.66LKK1022 pKa = 11.03VVATSGASTVEE1033 pKa = 3.98AVSANPVAGTDD1044 pKa = 3.49SLAGIVAKK1052 pKa = 10.6LDD1054 pKa = 3.72AEE1056 pKa = 4.37DD1057 pKa = 3.69WRR1059 pKa = 11.84PEE1061 pKa = 3.85PVYY1064 pKa = 11.22GEE1066 pKa = 4.19DD1067 pKa = 3.92ANVNDD1072 pKa = 3.69VLQAKK1077 pKa = 8.69LAEE1080 pKa = 4.25MGVDD1084 pKa = 3.46DD1085 pKa = 4.46VDD1087 pKa = 3.78VRR1089 pKa = 11.84VTGVAFVNQNEE1100 pKa = 4.24QASLGVSTANDD1111 pKa = 3.48ATNGDD1116 pKa = 3.25ITFFFIDD1123 pKa = 3.84PDD1125 pKa = 4.05VVTGFANYY1133 pKa = 7.39TQWRR1137 pKa = 11.84QLRR1140 pKa = 11.84LTFEE1144 pKa = 4.63LSRR1147 pKa = 11.84DD1148 pKa = 3.73GEE1150 pKa = 4.55TATYY1154 pKa = 10.55APGRR1158 pKa = 11.84SVIIPWDD1165 pKa = 3.45EE1166 pKa = 3.84ARR1168 pKa = 11.84VGEE1171 pKa = 4.3LLEE1174 pKa = 4.57EE1175 pKa = 4.32KK1176 pKa = 10.6AADD1179 pKa = 3.82LAIGFASGDD1188 pKa = 3.37TSNAVTGNLTLPYY1201 pKa = 9.66KK1202 pKa = 10.78LSDD1205 pKa = 3.35GSGDD1209 pKa = 3.41TRR1211 pKa = 11.84SWSSVSWSSDD1221 pKa = 2.71FDD1223 pKa = 3.59AVRR1226 pKa = 11.84ISGYY1230 pKa = 10.11GWSDD1234 pKa = 3.26YY1235 pKa = 10.73TGTVTRR1241 pKa = 11.84TAADD1245 pKa = 3.34QEE1247 pKa = 4.62VALTATIGVVASDD1260 pKa = 4.44GPSTTIEE1267 pKa = 3.82KK1268 pKa = 9.52TFTVTVLGDD1277 pKa = 3.54PALVEE1282 pKa = 4.33AEE1284 pKa = 3.99QAALQEE1290 pKa = 4.39KK1291 pKa = 10.16VDD1293 pKa = 3.74AAFTADD1299 pKa = 3.54ALTYY1303 pKa = 10.21SADD1306 pKa = 3.53GAAVDD1311 pKa = 3.96PAAVVGDD1318 pKa = 3.98LQLPRR1323 pKa = 11.84PSAIGIDD1330 pKa = 3.6GADD1333 pKa = 3.44YY1334 pKa = 9.69TITYY1338 pKa = 8.81AASTDD1343 pKa = 3.84AIEE1346 pKa = 4.19LNGYY1350 pKa = 8.83RR1351 pKa = 11.84GNVYY1355 pKa = 10.3QPLPDD1360 pKa = 4.43ADD1362 pKa = 3.95GAPVEE1367 pKa = 4.33LTLTVTSKK1375 pKa = 11.37ANPEE1379 pKa = 3.53ITASKK1384 pKa = 9.23TIQLTVAPLEE1394 pKa = 4.66AGDD1397 pKa = 3.83ITSEE1401 pKa = 4.07LALMEE1406 pKa = 4.35AAKK1409 pKa = 10.48AGYY1412 pKa = 8.79GQALLGEE1419 pKa = 4.56NASADD1424 pKa = 3.42AVTTNLHH1431 pKa = 6.39AFQKK1435 pKa = 10.81AYY1437 pKa = 10.83LDD1439 pKa = 4.06EE1440 pKa = 4.92NSVLTWAYY1448 pKa = 10.43DD1449 pKa = 3.37SAAAGVAGDD1458 pKa = 4.84GIVATDD1464 pKa = 3.89LPGYY1468 pKa = 10.6DD1469 pKa = 3.88PMGSAGWRR1477 pKa = 11.84LFRR1480 pKa = 11.84SSNAGVVAHH1489 pKa = 6.83EE1490 pKa = 4.63SLVVTQPTYY1499 pKa = 9.16NTEE1502 pKa = 3.9VTITSVLTSEE1512 pKa = 4.37KK1513 pKa = 8.5YY1514 pKa = 9.54ARR1516 pKa = 11.84YY1517 pKa = 9.9AEE1519 pKa = 4.76RR1520 pKa = 11.84YY1521 pKa = 9.91ADD1523 pKa = 3.97DD1524 pKa = 4.28EE1525 pKa = 4.59TWGPAFSLLANQEE1538 pKa = 3.74VSATFTVLGTSGEE1551 pKa = 4.24QNPSVTATVRR1561 pKa = 11.84IVGVDD1566 pKa = 3.17ASGVDD1571 pKa = 3.8EE1572 pKa = 4.58AWVVTTAYY1580 pKa = 9.96EE1581 pKa = 4.06LTEE1584 pKa = 4.39GSTAADD1590 pKa = 3.55LTEE1593 pKa = 4.69AALADD1598 pKa = 3.92AGLTADD1604 pKa = 4.05YY1605 pKa = 8.39GTGSRR1610 pKa = 11.84GWYY1613 pKa = 9.73LNSITSADD1621 pKa = 3.67GRR1623 pKa = 11.84VLEE1626 pKa = 4.26YY1627 pKa = 11.19DD1628 pKa = 3.82EE1629 pKa = 5.61DD1630 pKa = 3.94TGKK1633 pKa = 10.58YY1634 pKa = 7.01WQLFVNGSASDD1645 pKa = 3.47AGAGEE1650 pKa = 4.42VTLQAGDD1657 pKa = 4.16EE1658 pKa = 4.24ITWYY1662 pKa = 9.96YY1663 pKa = 9.51SAWGASLDD1671 pKa = 4.76DD1672 pKa = 3.89IGQARR1677 pKa = 11.84ISVTASVIGLDD1688 pKa = 4.41ADD1690 pKa = 4.17GEE1692 pKa = 4.38DD1693 pKa = 4.31ASWVPTTRR1701 pKa = 11.84VQLAEE1706 pKa = 4.2GSTAAEE1712 pKa = 4.11LTEE1715 pKa = 4.23EE1716 pKa = 4.6LLTSAGLEE1724 pKa = 4.14HH1725 pKa = 7.29EE1726 pKa = 4.37ATGVGTGGYY1735 pKa = 8.85YY1736 pKa = 10.78LSTITSTDD1744 pKa = 2.67GRR1746 pKa = 11.84TLGWDD1751 pKa = 3.75EE1752 pKa = 4.51DD1753 pKa = 4.34TGCYY1757 pKa = 7.66WQLFVNGSASDD1768 pKa = 3.48AGAGQVTLKK1777 pKa = 10.64PGDD1780 pKa = 3.87EE1781 pKa = 4.14IAWYY1785 pKa = 10.39YY1786 pKa = 9.54SAQGAEE1792 pKa = 4.4LPTNDD1797 pKa = 4.76VEE1799 pKa = 4.55VDD1801 pKa = 3.38PDD1803 pKa = 3.87APRR1806 pKa = 11.84PDD1808 pKa = 4.49YY1809 pKa = 11.07DD1810 pKa = 4.31AAWPTYY1816 pKa = 10.82APGAVTDD1823 pKa = 3.77VATPTEE1829 pKa = 4.4GGEE1832 pKa = 4.24LAWSVDD1838 pKa = 3.7LGTDD1842 pKa = 3.4MASSVYY1848 pKa = 10.6ASDD1851 pKa = 4.77PIVVNGNLYY1860 pKa = 10.52LAVGDD1865 pKa = 3.99EE1866 pKa = 4.55LRR1868 pKa = 11.84VLDD1871 pKa = 4.31AATGKK1876 pKa = 10.02ILATATLATSVDD1888 pKa = 3.89SACRR1892 pKa = 11.84LAYY1895 pKa = 10.19ADD1897 pKa = 3.83GLVVVPLHH1905 pKa = 6.34GGRR1908 pKa = 11.84LQALTADD1915 pKa = 3.8TLTTVSLTDD1924 pKa = 3.46EE1925 pKa = 4.4LAAGQQALSTLTISDD1940 pKa = 2.81GRR1942 pKa = 11.84AYY1944 pKa = 10.64FGTTSWTGEE1953 pKa = 4.16TGTLFCVEE1961 pKa = 4.73LATGAVVWSTSGDD1974 pKa = 3.42GDD1976 pKa = 4.5AGYY1979 pKa = 9.3YY1980 pKa = 8.36WCGAVLAGDD1989 pKa = 3.57WLVAVDD1995 pKa = 3.7GAGVVRR2001 pKa = 11.84AYY2003 pKa = 10.67DD2004 pKa = 3.54PATGEE2009 pKa = 4.07VAGTIDD2015 pKa = 4.03LGANAHH2021 pKa = 5.62VSVVADD2027 pKa = 3.93GSDD2030 pKa = 3.49LYY2032 pKa = 11.74VVTNDD2037 pKa = 3.4GTLHH2041 pKa = 6.63RR2042 pKa = 11.84LALAADD2048 pKa = 4.64GSLTQTGSVRR2058 pKa = 11.84FADD2061 pKa = 4.13SSTSTPTIADD2071 pKa = 2.85GRR2073 pKa = 11.84AYY2075 pKa = 10.87VGGANDD2081 pKa = 4.37DD2082 pKa = 3.95STGVLAVIDD2091 pKa = 4.34LATMGVEE2098 pKa = 3.85HH2099 pKa = 7.65RR2100 pKa = 11.84ITGFSNEE2107 pKa = 4.04AKK2109 pKa = 10.67LPGDD2113 pKa = 3.75VKK2115 pKa = 10.88SAPTVSARR2123 pKa = 11.84DD2124 pKa = 3.62GEE2126 pKa = 4.73TYY2128 pKa = 10.92VYY2130 pKa = 7.21FTCNAVSGGAYY2141 pKa = 10.35LYY2143 pKa = 10.84HH2144 pKa = 7.84LGDD2147 pKa = 3.36AHH2149 pKa = 7.55ASVLYY2154 pKa = 10.83LPGDD2158 pKa = 4.31DD2159 pKa = 4.19QADD2162 pKa = 3.21WCMASVVVGADD2173 pKa = 3.11GALYY2177 pKa = 10.09YY2178 pKa = 11.34VNDD2181 pKa = 3.65SGTLFKK2187 pKa = 11.26LEE2189 pKa = 4.64AGAALPDD2196 pKa = 3.98PTPTDD2201 pKa = 4.33DD2202 pKa = 5.02GDD2204 pKa = 3.76GTTGDD2209 pKa = 3.77NTNGSTSNTGDD2220 pKa = 3.23DD2221 pKa = 3.89TGDD2224 pKa = 3.32VDD2226 pKa = 5.96SGDD2229 pKa = 4.25DD2230 pKa = 3.79LDD2232 pKa = 5.88GGAHH2236 pKa = 6.66RR2237 pKa = 11.84PTGTVQPGRR2246 pKa = 11.84QPIATDD2252 pKa = 3.43DD2253 pKa = 3.96AAVDD2257 pKa = 3.88EE2258 pKa = 5.01AADD2261 pKa = 3.87DD2262 pKa = 4.3ALTEE2266 pKa = 4.3EE2267 pKa = 4.88TSSSDD2272 pKa = 3.22EE2273 pKa = 4.18TALSAGARR2281 pKa = 11.84VSSSADD2287 pKa = 2.85AGAAEE2292 pKa = 4.39QPRR2295 pKa = 11.84SFRR2298 pKa = 11.84WLPIAGIILGVCGLVVIIVYY2318 pKa = 8.14VKK2320 pKa = 10.34RR2321 pKa = 11.84RR2322 pKa = 11.84EE2323 pKa = 3.9HH2324 pKa = 7.48SEE2326 pKa = 3.73

Molecular weight:
240.81 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y3U203|A0A1Y3U203_9ACTN Uncharacterized protein OS=Enorma massiliensis OX=1472761 GN=B5G21_10080 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight:
5.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1937

0

1937

655953

25

3004

338.6

36.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.872 ± 0.084

1.504 ± 0.023

6.23 ± 0.045

6.866 ± 0.054

3.584 ± 0.035

8.279 ± 0.049

1.994 ± 0.026

5.136 ± 0.045

3.24 ± 0.047

9.123 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.574 ± 0.025

2.733 ± 0.039

4.374 ± 0.035

2.886 ± 0.028

6.559 ± 0.068

5.982 ± 0.05

5.365 ± 0.057

7.86 ± 0.043

1.029 ± 0.023

2.809 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski