HCBI9.212 virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykrogvirus; Gemykrogvirus bovas1; Bovine associated gemykrogvirus 1

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077XNA4|A0A077XNA4_9VIRU Replication associated protein OS=HCBI9.212 virus OX=1516080 GN=rep PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 5.2EE3 pKa = 5.56FSDD6 pKa = 3.8DD7 pKa = 3.12CQYY10 pKa = 12.03AVFDD14 pKa = 5.26DD15 pKa = 3.78IQGGFEE21 pKa = 4.13YY22 pKa = 10.4FHH24 pKa = 7.24SYY26 pKa = 10.2KK27 pKa = 10.66GWLGAQKK34 pKa = 10.48QFVITDD40 pKa = 3.92KK41 pKa = 10.7YY42 pKa = 9.41RR43 pKa = 11.84KK44 pKa = 9.57KK45 pKa = 10.0RR46 pKa = 11.84TIYY49 pKa = 8.85WGKK52 pKa = 9.5PSIMCMNEE60 pKa = 3.78DD61 pKa = 3.13PHH63 pKa = 6.07FAKK66 pKa = 10.77GVDD69 pKa = 3.94YY70 pKa = 10.96DD71 pKa = 3.44WLMNNCHH78 pKa = 7.1IINVVDD84 pKa = 4.89PICIVSNPPSSNEE97 pKa = 4.04SQSEE101 pKa = 4.24QII103 pKa = 4.15

Molecular weight: 12.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077XLW1|A0A077XLW1_9VIRU Replication protein OS=HCBI9.212 virus OX=1516080 GN=Rep PE=3 SV=1
MM1 pKa = 7.83AYY3 pKa = 10.26GFRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84FGRR11 pKa = 11.84SRR13 pKa = 11.84RR14 pKa = 11.84SYY16 pKa = 9.2RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84PVRR22 pKa = 11.84RR23 pKa = 11.84YY24 pKa = 8.9SRR26 pKa = 11.84RR27 pKa = 11.84GRR29 pKa = 11.84SGYY32 pKa = 10.52GRR34 pKa = 11.84GGYY37 pKa = 7.78NRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84YY42 pKa = 9.23RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84SWISPYY53 pKa = 10.49NKK55 pKa = 9.56KK56 pKa = 10.1HH57 pKa = 6.05DD58 pKa = 4.48VIKK61 pKa = 10.53GAHH64 pKa = 6.95DD65 pKa = 4.0SNDD68 pKa = 3.11NSFAPIGAPNTFFGFCPTYY87 pKa = 10.07MPNAVAGDD95 pKa = 3.86TGHH98 pKa = 6.52NPQKK102 pKa = 10.72VRR104 pKa = 11.84TSKK107 pKa = 10.64NVGFSGYY114 pKa = 7.18QEE116 pKa = 3.84RR117 pKa = 11.84VHH119 pKa = 6.45IATAEE124 pKa = 4.03SLIWRR129 pKa = 11.84RR130 pKa = 11.84MVIWSYY136 pKa = 11.7NRR138 pKa = 11.84LEE140 pKa = 4.22NTAGPTKK147 pKa = 10.39TDD149 pKa = 3.57DD150 pKa = 3.17QGTSYY155 pKa = 7.46TTRR158 pKa = 11.84QLTPLQLNEE167 pKa = 3.98GVRR170 pKa = 11.84AFLFRR175 pKa = 11.84GTQGIDD181 pKa = 3.19YY182 pKa = 9.88TEE184 pKa = 4.25NTLHH188 pKa = 6.46EE189 pKa = 4.74APLNSDD195 pKa = 4.15HH196 pKa = 6.53FTVAYY201 pKa = 10.3DD202 pKa = 3.51RR203 pKa = 11.84TRR205 pKa = 11.84VLQPRR210 pKa = 11.84RR211 pKa = 11.84PTGDD215 pKa = 2.99EE216 pKa = 3.82YY217 pKa = 12.03GHH219 pKa = 6.44IFNMKK224 pKa = 8.4FWNPGGRR231 pKa = 11.84IIYY234 pKa = 10.4SDD236 pKa = 5.34DD237 pKa = 3.44EE238 pKa = 6.41DD239 pKa = 4.55GLQKK243 pKa = 11.07GPLVNGWSSLGRR255 pKa = 11.84VSKK258 pKa = 11.21GNMYY262 pKa = 10.44IFDD265 pKa = 4.18VFSTGYY271 pKa = 9.78SLSSNTPSIARR282 pKa = 11.84FAPTGTRR289 pKa = 11.84YY290 pKa = 6.29WTEE293 pKa = 3.8GG294 pKa = 3.14

Molecular weight: 33.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

641

103

294

213.7

24.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.148 ± 0.79

1.872 ± 0.94

6.24 ± 1.094

5.148 ± 1.395

4.992 ± 0.28

7.956 ± 1.551

2.964 ± 0.386

6.24 ± 1.307

3.9 ± 0.811

4.68 ± 0.884

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.872 ± 0.557

5.46 ± 0.483

5.772 ± 0.405

3.744 ± 0.706

10.14 ± 2.471

6.864 ± 0.768

5.148 ± 1.624

4.524 ± 0.361

1.872 ± 0.363

5.46 ± 0.483
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski