Bhendi yellow vein mosaic betasatellite [India:Coimbator:OYCO1:2005]
Average proteome isoelectric point is 5.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7BNB9|E7BNB9_9VIRU C1 protein OS=Bhendi yellow vein mosaic betasatellite [India:Coimbator:OYCO1:2005] OX=908072 GN=C1 PE=4 SV=1
MM1 pKa = 7.23 TRR3 pKa = 11.84 FSRR6 pKa = 11.84 TRR8 pKa = 11.84 EE9 pKa = 4.11 GIVIRR14 pKa = 11.84 EE15 pKa = 4.25 DD16 pKa = 3.41 VRR18 pKa = 11.84 DD19 pKa = 3.77 KK20 pKa = 10.33 QQQRR24 pKa = 11.84 ASVHH28 pKa = 6.12 KK29 pKa = 9.87 YY30 pKa = 9.09 ISSAKK35 pKa = 8.18 WSSHH39 pKa = 3.5 ITKK42 pKa = 10.59 AFIVDD47 pKa = 3.13 YY48 pKa = 10.38 AYY50 pKa = 10.64 QQLHH54 pKa = 6.35 IPFDD58 pKa = 3.77 FTGVEE63 pKa = 4.25 GEE65 pKa = 4.01 ITSTFKK71 pKa = 10.2 FHH73 pKa = 6.13 YY74 pKa = 8.58 WGSKK78 pKa = 9.77 AEE80 pKa = 4.51 EE81 pKa = 4.2 ILEE84 pKa = 3.84 EE85 pKa = 4.9 DD86 pKa = 3.96 IIHH89 pKa = 5.76 VVDD92 pKa = 5.04 IIIMEE97 pKa = 4.49 NPDD100 pKa = 3.32 IMVMEE105 pKa = 4.29 VNEE108 pKa = 3.92 PVIIDD113 pKa = 3.61 SNIIII118 pKa = 4.5
Molecular weight: 13.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.057
IPC2_protein 5.08
IPC_protein 4.978
Toseland 4.978
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.876
Rodwell 4.914
Grimsley 4.914
Solomon 4.991
Lehninger 4.952
Nozaki 5.13
DTASelect 5.27
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.207
Patrickios 4.113
IPC_peptide 5.003
IPC2_peptide 5.207
IPC2.peptide.svr19 5.15
Protein with the highest isoelectric point:
>tr|E7BNB9|E7BNB9_9VIRU C1 protein OS=Bhendi yellow vein mosaic betasatellite [India:Coimbator:OYCO1:2005] OX=908072 GN=C1 PE=4 SV=1
MM1 pKa = 7.23 TRR3 pKa = 11.84 FSRR6 pKa = 11.84 TRR8 pKa = 11.84 EE9 pKa = 4.11 GIVIRR14 pKa = 11.84 EE15 pKa = 4.25 DD16 pKa = 3.41 VRR18 pKa = 11.84 DD19 pKa = 3.77 KK20 pKa = 10.33 QQQRR24 pKa = 11.84 ASVHH28 pKa = 6.12 KK29 pKa = 9.87 YY30 pKa = 9.09 ISSAKK35 pKa = 8.18 WSSHH39 pKa = 3.5 ITKK42 pKa = 10.59 AFIVDD47 pKa = 3.13 YY48 pKa = 10.38 AYY50 pKa = 10.64 QQLHH54 pKa = 6.35 IPFDD58 pKa = 3.77 FTGVEE63 pKa = 4.25 GEE65 pKa = 4.01 ITSTFKK71 pKa = 10.2 FHH73 pKa = 6.13 YY74 pKa = 8.58 WGSKK78 pKa = 9.77 AEE80 pKa = 4.51 EE81 pKa = 4.2 ILEE84 pKa = 3.84 EE85 pKa = 4.9 DD86 pKa = 3.96 IIHH89 pKa = 5.76 VVDD92 pKa = 5.04 IIIMEE97 pKa = 4.49 NPDD100 pKa = 3.32 IMVMEE105 pKa = 4.29 VNEE108 pKa = 3.92 PVIIDD113 pKa = 3.61 SNIIII118 pKa = 4.5
Molecular weight: 13.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.057
IPC2_protein 5.08
IPC_protein 4.978
Toseland 4.978
ProMoST 5.143
Dawson 4.991
Bjellqvist 5.118
Wikipedia 4.876
Rodwell 4.914
Grimsley 4.914
Solomon 4.991
Lehninger 4.952
Nozaki 5.13
DTASelect 5.27
Thurlkill 4.978
EMBOSS 4.927
Sillero 5.207
Patrickios 4.113
IPC_peptide 5.003
IPC2_peptide 5.207
IPC2.peptide.svr19 5.15
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.237 ± 0.0
0.0 ± 0.0
6.78 ± 0.0
9.322 ± 0.0
5.085 ± 0.0
3.39 ± 0.0
4.237 ± 0.0
16.102 ± 0.0
5.085 ± 0.0
1.695 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.39 ± 0.0
2.542 ± 0.0
2.542 ± 0.0
4.237 ± 0.0
5.085 ± 0.0
7.627 ± 0.0
5.085 ± 0.0
8.475 ± 0.0
1.695 ± 0.0
3.39 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here