Brachybacterium sp. UMB0905

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Dermabacteraceae; Brachybacterium; unclassified Brachybacterium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2790 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N6TZ05|A0A2N6TZ05_9MICO AAA family ATPase OS=Brachybacterium sp. UMB0905 OX=2069310 GN=CJ197_13115 PE=4 SV=1
MM1 pKa = 7.85DD2 pKa = 3.49TTDD5 pKa = 3.23PRR7 pKa = 11.84RR8 pKa = 11.84VGRR11 pKa = 11.84PYY13 pKa = 10.6PEE15 pKa = 4.44ARR17 pKa = 11.84EE18 pKa = 3.87HH19 pKa = 5.71TAAQNSAGAVPSGTAPRR36 pKa = 11.84NSFEE40 pKa = 3.97GRR42 pKa = 11.84PLHH45 pKa = 7.0HH46 pKa = 7.2PAEE49 pKa = 4.53DD50 pKa = 4.04VEE52 pKa = 4.38DD53 pKa = 3.46QGLAFDD59 pKa = 3.99VGTLVSRR66 pKa = 11.84RR67 pKa = 11.84GALGVLGAGSVTAVLAACTGGSASTTAAASDD98 pKa = 3.67GGGASDD104 pKa = 5.05AGGTTASEE112 pKa = 4.18LTEE115 pKa = 4.35MPGEE119 pKa = 4.21TAGPYY124 pKa = 10.16PGDD127 pKa = 3.75GSNGADD133 pKa = 3.27VLEE136 pKa = 4.38TSGVEE141 pKa = 3.72RR142 pKa = 11.84SDD144 pKa = 2.95IRR146 pKa = 11.84SSIDD150 pKa = 2.73GGTTVEE156 pKa = 5.05GVPLDD161 pKa = 3.31ITMTIIDD168 pKa = 4.1MAGGDD173 pKa = 3.9VPMEE177 pKa = 4.22GAAVYY182 pKa = 9.55LWQCDD187 pKa = 3.16AAGRR191 pKa = 11.84YY192 pKa = 9.26SMYY195 pKa = 10.69SEE197 pKa = 4.04GVEE200 pKa = 3.9EE201 pKa = 3.99EE202 pKa = 4.51TYY204 pKa = 11.11LRR206 pKa = 11.84GVQIADD212 pKa = 3.23AQGAVTFTSIFPGCYY227 pKa = 9.05AGRR230 pKa = 11.84WPHH233 pKa = 5.89LHH235 pKa = 6.73FEE237 pKa = 4.35VFPDD241 pKa = 3.5AEE243 pKa = 4.64SIVDD247 pKa = 3.61AGNAILTSQIAMPEE261 pKa = 3.85AEE263 pKa = 4.07ADD265 pKa = 3.58GVYY268 pKa = 10.77EE269 pKa = 4.0LTEE272 pKa = 4.07YY273 pKa = 10.93AGSAEE278 pKa = 4.23NLSQLTLEE286 pKa = 4.3TDD288 pKa = 3.51NVFSDD293 pKa = 4.99GYY295 pKa = 8.66EE296 pKa = 3.91QQLATLSGDD305 pKa = 3.57LDD307 pKa = 3.45SGYY310 pKa = 10.94AFTIDD315 pKa = 3.66VPIDD319 pKa = 3.37TTTEE323 pKa = 4.06PEE325 pKa = 3.96VGGMGGGEE333 pKa = 4.44GGPGGQPPADD343 pKa = 3.79GEE345 pKa = 4.36GGPDD349 pKa = 3.8GEE351 pKa = 5.21GGTPPEE357 pKa = 4.45GAPGEE362 pKa = 4.49GGEE365 pKa = 4.62PPADD369 pKa = 3.69APSDD373 pKa = 3.75GGGDD377 pKa = 3.67TQSS380 pKa = 3.0

Molecular weight: 38.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N6U448|A0A2N6U448_9MICO Catalase OS=Brachybacterium sp. UMB0905 OX=2069310 GN=CJ197_04185 PE=3 SV=1
MM1 pKa = 7.28GSVVKK6 pKa = 10.44KK7 pKa = 9.48RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.32RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.31LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2790

0

2790

933859

24

2077

334.7

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.965 ± 0.07

0.564 ± 0.01

6.14 ± 0.038

6.414 ± 0.047

2.657 ± 0.028

8.896 ± 0.044

2.263 ± 0.022

4.266 ± 0.036

1.812 ± 0.033

10.527 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.03 ± 0.017

1.694 ± 0.021

5.852 ± 0.037

3.384 ± 0.023

7.61 ± 0.055

5.337 ± 0.03

6.197 ± 0.032

8.151 ± 0.036

1.426 ± 0.02

1.816 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski