Orungo virus
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5QLX5|W5QLX5_9REOV RNA-directed RNA polymerase OS=Orungo virus OX=40058 PE=3 SV=1
MM1 pKa = 6.86 AQEE4 pKa = 4.38 VKK6 pKa = 10.53 RR7 pKa = 11.84 KK8 pKa = 6.98 FTRR11 pKa = 11.84 TVCIYY16 pKa = 10.51 DD17 pKa = 3.37 PQNRR21 pKa = 11.84 TFCGRR26 pKa = 11.84 SAAMVCNSYY35 pKa = 8.95 YY36 pKa = 8.37 TIKK39 pKa = 9.97 MGRR42 pKa = 11.84 TTQCGVSPVPVPKK55 pKa = 10.32 SYY57 pKa = 11.14 VLEE60 pKa = 4.73 IPDD63 pKa = 3.46 VGSYY67 pKa = 10.56 RR68 pKa = 11.84 ILDD71 pKa = 4.11 GQDD74 pKa = 3.49 SISVIVTEE82 pKa = 4.53 TGVEE86 pKa = 4.05 ATSEE90 pKa = 3.87 RR91 pKa = 11.84 WEE93 pKa = 3.79 EE94 pKa = 3.88 WKK96 pKa = 10.95 FEE98 pKa = 3.93 GVNCVPMVVQTEE110 pKa = 4.11 IGKK113 pKa = 9.94 GLIDD117 pKa = 3.73 MEE119 pKa = 4.59 IKK121 pKa = 10.48 FSKK124 pKa = 10.67 GMGLVKK130 pKa = 10.09 PYY132 pKa = 10.76 VKK134 pKa = 10.76 NEE136 pKa = 3.66 VSRR139 pKa = 11.84 TEE141 pKa = 3.78 IPRR144 pKa = 11.84 LPGASVIDD152 pKa = 3.87 TPIRR156 pKa = 11.84 DD157 pKa = 3.6 YY158 pKa = 11.24 RR159 pKa = 11.84 QLLKK163 pKa = 10.63 DD164 pKa = 3.3 QRR166 pKa = 11.84 DD167 pKa = 3.63 EE168 pKa = 4.03 RR169 pKa = 11.84 RR170 pKa = 11.84 AALDD174 pKa = 5.02 AIACDD179 pKa = 3.32 ICPTLSRR186 pKa = 11.84 MLGANITDD194 pKa = 4.22 GSPQVEE200 pKa = 4.38 EE201 pKa = 4.27 VATRR205 pKa = 11.84 PTQPPAQLKK214 pKa = 10.52 LDD216 pKa = 3.86 VPNWDD221 pKa = 3.82 QPTDD225 pKa = 3.62 SDD227 pKa = 5.97 DD228 pKa = 3.64 EE229 pKa = 4.76 DD230 pKa = 4.22 EE231 pKa = 4.2 EE232 pKa = 4.85 SKK234 pKa = 10.97 RR235 pKa = 11.84 VTTQSNADD243 pKa = 3.54 TEE245 pKa = 4.44 EE246 pKa = 3.88 QVTFEE251 pKa = 5.18 DD252 pKa = 5.76 CITDD256 pKa = 3.42 QYY258 pKa = 11.25 FSKK261 pKa = 9.83 TGEE264 pKa = 3.94 FAKK267 pKa = 10.3 KK268 pKa = 9.42 YY269 pKa = 9.89 PEE271 pKa = 3.95 MLAGLSAKK279 pKa = 9.52 MPSEE283 pKa = 3.88 TGRR286 pKa = 11.84 FTKK289 pKa = 10.33 ILTTRR294 pKa = 11.84 KK295 pKa = 9.36 AQWNNVPLFEE305 pKa = 6.23 LDD307 pKa = 3.67 PNGFTYY313 pKa = 10.48 HH314 pKa = 6.68 FSKK317 pKa = 10.68 LGNSTRR323 pKa = 11.84 IFCVQRR329 pKa = 11.84 DD330 pKa = 4.06 LSYY333 pKa = 10.37 MVLPAGQGLKK343 pKa = 10.5
Molecular weight: 38.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.1
IPC2_protein 5.219
IPC_protein 5.13
Toseland 4.978
ProMoST 5.219
Dawson 5.067
Bjellqvist 5.219
Wikipedia 4.927
Rodwell 4.978
Grimsley 4.889
Solomon 5.067
Lehninger 5.016
Nozaki 5.181
DTASelect 5.334
Thurlkill 4.978
EMBOSS 4.952
Sillero 5.245
Patrickios 4.151
IPC_peptide 5.08
IPC2_peptide 5.232
IPC2.peptide.svr19 5.325
Protein with the highest isoelectric point:
>tr|W5QM02|W5QM02_9REOV NS4 OS=Orungo virus OX=40058 PE=4 SV=1
MM1 pKa = 6.37 MTSKK5 pKa = 10.74 RR6 pKa = 11.84 MNQWIRR12 pKa = 11.84 EE13 pKa = 4.06 AASRR17 pKa = 11.84 VIEE20 pKa = 4.18 SGRR23 pKa = 11.84 KK24 pKa = 9.45 LEE26 pKa = 3.99 MPRR29 pKa = 11.84 VNSLPIKK36 pKa = 9.89 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 LEE41 pKa = 3.67 EE42 pKa = 3.66 VRR44 pKa = 11.84 IQSSGKK50 pKa = 9.86 VEE52 pKa = 3.9 MEE54 pKa = 3.55 GDD56 pKa = 3.0 KK57 pKa = 10.89 LRR59 pKa = 11.84 RR60 pKa = 11.84 LKK62 pKa = 10.83 AEE64 pKa = 3.87 RR65 pKa = 11.84 MEE67 pKa = 4.25 LEE69 pKa = 3.67 ILAFRR74 pKa = 11.84 IKK76 pKa = 9.84 TNNLIGALIQITRR89 pKa = 11.84 WTEE92 pKa = 3.88 KK93 pKa = 9.5 IDD95 pKa = 3.84 QEE97 pKa = 4.41 IRR99 pKa = 11.84 NLNMDD104 pKa = 4.44 LQPNEE109 pKa = 4.15 LQTPLSQSTTAVDD122 pKa = 4.36 LRR124 pKa = 11.84 GQDD127 pKa = 4.94 RR128 pKa = 11.84 MDD130 pKa = 3.38 TSNN133 pKa = 3.3
Molecular weight: 15.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.104
IPC2_protein 9.165
IPC_protein 9.341
Toseland 10.54
ProMoST 10.014
Dawson 10.584
Bjellqvist 10.16
Wikipedia 10.701
Rodwell 10.891
Grimsley 10.584
Solomon 10.687
Lehninger 10.672
Nozaki 10.511
DTASelect 10.16
Thurlkill 10.511
EMBOSS 10.921
Sillero 10.511
Patrickios 10.672
IPC_peptide 10.701
IPC2_peptide 8.434
IPC2.peptide.svr19 8.503
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
6209
133
1302
564.5
64.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.022 ± 0.621
0.902 ± 0.223
6.056 ± 0.303
6.684 ± 0.482
4.252 ± 0.521
6.265 ± 0.418
2.174 ± 0.339
6.507 ± 0.331
5.138 ± 0.559
9.019 ± 0.386
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.656 ± 0.258
3.769 ± 0.278
3.994 ± 0.379
3.64 ± 0.311
7.57 ± 0.426
5.991 ± 0.467
5.589 ± 0.278
7.103 ± 0.247
1.305 ± 0.213
3.366 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here