Orungo virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|W5QLX5|W5QLX5_9REOV RNA-directed RNA polymerase OS=Orungo virus OX=40058 PE=3 SV=1
MM1 pKa = 6.86AQEE4 pKa = 4.38VKK6 pKa = 10.53RR7 pKa = 11.84KK8 pKa = 6.98FTRR11 pKa = 11.84TVCIYY16 pKa = 10.51DD17 pKa = 3.37PQNRR21 pKa = 11.84TFCGRR26 pKa = 11.84SAAMVCNSYY35 pKa = 8.95YY36 pKa = 8.37TIKK39 pKa = 9.97MGRR42 pKa = 11.84TTQCGVSPVPVPKK55 pKa = 10.32SYY57 pKa = 11.14VLEE60 pKa = 4.73IPDD63 pKa = 3.46VGSYY67 pKa = 10.56RR68 pKa = 11.84ILDD71 pKa = 4.11GQDD74 pKa = 3.49SISVIVTEE82 pKa = 4.53TGVEE86 pKa = 4.05ATSEE90 pKa = 3.87RR91 pKa = 11.84WEE93 pKa = 3.79EE94 pKa = 3.88WKK96 pKa = 10.95FEE98 pKa = 3.93GVNCVPMVVQTEE110 pKa = 4.11IGKK113 pKa = 9.94GLIDD117 pKa = 3.73MEE119 pKa = 4.59IKK121 pKa = 10.48FSKK124 pKa = 10.67GMGLVKK130 pKa = 10.09PYY132 pKa = 10.76VKK134 pKa = 10.76NEE136 pKa = 3.66VSRR139 pKa = 11.84TEE141 pKa = 3.78IPRR144 pKa = 11.84LPGASVIDD152 pKa = 3.87TPIRR156 pKa = 11.84DD157 pKa = 3.6YY158 pKa = 11.24RR159 pKa = 11.84QLLKK163 pKa = 10.63DD164 pKa = 3.3QRR166 pKa = 11.84DD167 pKa = 3.63EE168 pKa = 4.03RR169 pKa = 11.84RR170 pKa = 11.84AALDD174 pKa = 5.02AIACDD179 pKa = 3.32ICPTLSRR186 pKa = 11.84MLGANITDD194 pKa = 4.22GSPQVEE200 pKa = 4.38EE201 pKa = 4.27VATRR205 pKa = 11.84PTQPPAQLKK214 pKa = 10.52LDD216 pKa = 3.86VPNWDD221 pKa = 3.82QPTDD225 pKa = 3.62SDD227 pKa = 5.97DD228 pKa = 3.64EE229 pKa = 4.76DD230 pKa = 4.22EE231 pKa = 4.2EE232 pKa = 4.85SKK234 pKa = 10.97RR235 pKa = 11.84VTTQSNADD243 pKa = 3.54TEE245 pKa = 4.44EE246 pKa = 3.88QVTFEE251 pKa = 5.18DD252 pKa = 5.76CITDD256 pKa = 3.42QYY258 pKa = 11.25FSKK261 pKa = 9.83TGEE264 pKa = 3.94FAKK267 pKa = 10.3KK268 pKa = 9.42YY269 pKa = 9.89PEE271 pKa = 3.95MLAGLSAKK279 pKa = 9.52MPSEE283 pKa = 3.88TGRR286 pKa = 11.84FTKK289 pKa = 10.33ILTTRR294 pKa = 11.84KK295 pKa = 9.36AQWNNVPLFEE305 pKa = 6.23LDD307 pKa = 3.67PNGFTYY313 pKa = 10.48HH314 pKa = 6.68FSKK317 pKa = 10.68LGNSTRR323 pKa = 11.84IFCVQRR329 pKa = 11.84DD330 pKa = 4.06LSYY333 pKa = 10.37MVLPAGQGLKK343 pKa = 10.5

Molecular weight:
38.53 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|W5QM02|W5QM02_9REOV NS4 OS=Orungo virus OX=40058 PE=4 SV=1
MM1 pKa = 6.37MTSKK5 pKa = 10.74RR6 pKa = 11.84MNQWIRR12 pKa = 11.84EE13 pKa = 4.06AASRR17 pKa = 11.84VIEE20 pKa = 4.18SGRR23 pKa = 11.84KK24 pKa = 9.45LEE26 pKa = 3.99MPRR29 pKa = 11.84VNSLPIKK36 pKa = 9.89RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84LEE41 pKa = 3.67EE42 pKa = 3.66VRR44 pKa = 11.84IQSSGKK50 pKa = 9.86VEE52 pKa = 3.9MEE54 pKa = 3.55GDD56 pKa = 3.0KK57 pKa = 10.89LRR59 pKa = 11.84RR60 pKa = 11.84LKK62 pKa = 10.83AEE64 pKa = 3.87RR65 pKa = 11.84MEE67 pKa = 4.25LEE69 pKa = 3.67ILAFRR74 pKa = 11.84IKK76 pKa = 9.84TNNLIGALIQITRR89 pKa = 11.84WTEE92 pKa = 3.88KK93 pKa = 9.5IDD95 pKa = 3.84QEE97 pKa = 4.41IRR99 pKa = 11.84NLNMDD104 pKa = 4.44LQPNEE109 pKa = 4.15LQTPLSQSTTAVDD122 pKa = 4.36LRR124 pKa = 11.84GQDD127 pKa = 4.94RR128 pKa = 11.84MDD130 pKa = 3.38TSNN133 pKa = 3.3

Molecular weight:
15.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

6209

133

1302

564.5

64.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.022 ± 0.621

0.902 ± 0.223

6.056 ± 0.303

6.684 ± 0.482

4.252 ± 0.521

6.265 ± 0.418

2.174 ± 0.339

6.507 ± 0.331

5.138 ± 0.559

9.019 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.656 ± 0.258

3.769 ± 0.278

3.994 ± 0.379

3.64 ± 0.311

7.57 ± 0.426

5.991 ± 0.467

5.589 ± 0.278

7.103 ± 0.247

1.305 ± 0.213

3.366 ± 0.342

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski