Nitrosomonas nitrosa

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4UP56|A0A1I4UP56_9PROT Uncharacterized protein OS=Nitrosomonas nitrosa OX=52442 GN=SAMN05421880_1503 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.15KK3 pKa = 9.67IRR5 pKa = 11.84LICMIIALAGCLSAGPIWSADD26 pKa = 3.43GEE28 pKa = 4.64GGGDD32 pKa = 3.56FGGDD36 pKa = 3.25FDD38 pKa = 5.51GGGDD42 pKa = 3.57FGGDD46 pKa = 3.37FDD48 pKa = 5.75SSGDD52 pKa = 3.51FGGDD56 pKa = 2.93FDD58 pKa = 5.26YY59 pKa = 11.7GGDD62 pKa = 3.61FSGDD66 pKa = 3.14FDD68 pKa = 4.99SDD70 pKa = 3.79YY71 pKa = 11.69GGDD74 pKa = 3.45FGGDD78 pKa = 3.3FDD80 pKa = 4.93QGSEE84 pKa = 3.9FDD86 pKa = 3.36QGGEE90 pKa = 3.94FDD92 pKa = 3.45QGSDD96 pKa = 3.52FEE98 pKa = 4.84QDD100 pKa = 4.0FDD102 pKa = 3.72QQDD105 pKa = 3.49YY106 pKa = 10.69FGQEE110 pKa = 3.85EE111 pKa = 4.13FDD113 pKa = 3.67QDD115 pKa = 3.17GSEE118 pKa = 4.29YY119 pKa = 11.11GSVDD123 pKa = 3.21PNEE126 pKa = 5.7AFDD129 pKa = 3.41QDD131 pKa = 3.68NRR133 pKa = 11.84FDD135 pKa = 3.64QQQDD139 pKa = 3.39FEE141 pKa = 4.56FADD144 pKa = 4.86DD145 pKa = 3.98SDD147 pKa = 3.79QAGDD151 pKa = 3.86FDD153 pKa = 5.34QNDD156 pKa = 4.31DD157 pKa = 4.37FDD159 pKa = 3.95QADD162 pKa = 3.73NFDD165 pKa = 4.16PQNDD169 pKa = 3.51ASLEE173 pKa = 4.33SNADD177 pKa = 3.54PVIEE181 pKa = 4.21SAPEE185 pKa = 3.61EE186 pKa = 4.53VLTQDD191 pKa = 3.93EE192 pKa = 5.09DD193 pKa = 3.96PAQEE197 pKa = 4.17DD198 pKa = 3.76QSTGMSEE205 pKa = 3.79PAQSNEE211 pKa = 3.67FALDD215 pKa = 3.8NADD218 pKa = 3.68TDD220 pKa = 4.02NKK222 pKa = 10.01STEE225 pKa = 4.01TDD227 pKa = 3.19QPRR230 pKa = 11.84DD231 pKa = 3.65DD232 pKa = 4.86PVAQSAGEE240 pKa = 4.05PNPEE244 pKa = 4.58SIEE247 pKa = 4.0TDD249 pKa = 3.26QVDD252 pKa = 3.98TVSEE256 pKa = 4.39STQTLPSQQEE266 pKa = 3.82DD267 pKa = 3.34KK268 pKa = 11.17AAEE271 pKa = 4.07NDD273 pKa = 3.62GLAQTGDD280 pKa = 3.47SALPDD285 pKa = 3.16QSLQSDD291 pKa = 4.11EE292 pKa = 4.63SSQAEE297 pKa = 4.05PTAQGEE303 pKa = 4.47DD304 pKa = 3.31ASQPTQSAQAGSDD317 pKa = 3.28AAGNPATQNDD327 pKa = 3.76VTDD330 pKa = 4.1QNDD333 pKa = 3.52TSNQNINIQNVQTVQNNGHH352 pKa = 6.13DD353 pKa = 3.62HH354 pKa = 6.48PVGHH358 pKa = 6.44GHH360 pKa = 6.76GGWHH364 pKa = 5.73GHH366 pKa = 4.85HH367 pKa = 7.02HH368 pKa = 5.94GHH370 pKa = 6.15HH371 pKa = 6.32HH372 pKa = 6.06HH373 pKa = 7.08HH374 pKa = 6.59GFDD377 pKa = 2.91IGFGLGLGLGLGWSAFSPFFPYY399 pKa = 10.7APVVPFAGFSYY410 pKa = 10.08GWGGFAPYY418 pKa = 10.19SSFGIYY424 pKa = 10.06SGFGSFGGVGFYY436 pKa = 11.09SNYY439 pKa = 9.8VPFGGVSLYY448 pKa = 10.71SGYY451 pKa = 11.2SRR453 pKa = 11.84FRR455 pKa = 11.84SFNRR459 pKa = 11.84FGGYY463 pKa = 9.45YY464 pKa = 9.13PPLGGFPIVAAAPPILPVPVMIAPPRR490 pKa = 11.84KK491 pKa = 7.63PTYY494 pKa = 7.62IQKK497 pKa = 10.21RR498 pKa = 11.84SVSRR502 pKa = 11.84PPPAEE507 pKa = 3.51NSYY510 pKa = 9.27YY511 pKa = 9.7WHH513 pKa = 6.8YY514 pKa = 10.73CRR516 pKa = 11.84NPAGYY521 pKa = 9.35YY522 pKa = 9.71PYY524 pKa = 10.76VRR526 pKa = 11.84KK527 pKa = 10.21CPGGWIKK534 pKa = 10.68VPPQPSS540 pKa = 2.89

Molecular weight: 58.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4PZL8|A0A1I4PZL8_9PROT Uncharacterized protein OS=Nitrosomonas nitrosa OX=52442 GN=SAMN05421880_11328 PE=4 SV=1
MM1 pKa = 7.85IDD3 pKa = 3.63FTSALYY9 pKa = 10.62LGLHH13 pKa = 6.0HH14 pKa = 7.35PSRR17 pKa = 11.84LLQPWQQLTLGMPAALAEE35 pKa = 4.29PPGAAEE41 pKa = 4.32VAQKK45 pKa = 10.5LARR48 pKa = 11.84LQGCEE53 pKa = 4.22RR54 pKa = 11.84GVLMPSTLHH63 pKa = 6.74LFWDD67 pKa = 4.74LFGMLCRR74 pKa = 11.84QPVTLYY80 pKa = 10.85LDD82 pKa = 3.59EE83 pKa = 4.3GTYY86 pKa = 10.64AIARR90 pKa = 11.84WGMARR95 pKa = 11.84LSARR99 pKa = 11.84GVAIHH104 pKa = 6.76PFRR107 pKa = 11.84HH108 pKa = 6.05HH109 pKa = 7.36DD110 pKa = 3.83ANALLTKK117 pKa = 9.68ITRR120 pKa = 11.84QARR123 pKa = 11.84NRR125 pKa = 11.84KK126 pKa = 8.6YY127 pKa = 10.24PVVVANGFCPACGTAAPIGDD147 pKa = 3.87YY148 pKa = 11.01LDD150 pKa = 3.01IVRR153 pKa = 11.84RR154 pKa = 11.84FDD156 pKa = 3.44GLLVLDD162 pKa = 4.37DD163 pKa = 4.02TQALGVLGEE172 pKa = 4.26APALRR177 pKa = 11.84RR178 pKa = 11.84PYY180 pKa = 10.83GLGGGGILRR189 pKa = 11.84WSGHH193 pKa = 5.37TGPDD197 pKa = 2.98ILVGSSLAKK206 pKa = 10.46GLGVPVAVLTGSDD219 pKa = 2.91RR220 pKa = 11.84LLRR223 pKa = 11.84RR224 pKa = 11.84FTVASDD230 pKa = 3.49TRR232 pKa = 11.84VHH234 pKa = 6.92SSPPSLAVIQAAIHH248 pKa = 5.68ALAVNRR254 pKa = 11.84LQGNLLRR261 pKa = 11.84LRR263 pKa = 11.84LAQRR267 pKa = 11.84VGQFRR272 pKa = 11.84RR273 pKa = 11.84GLAGIGWSSTGGWFPVQTLCGITEE297 pKa = 3.98KK298 pKa = 10.97VAIRR302 pKa = 11.84LHH304 pKa = 5.55QRR306 pKa = 11.84LAQWGVQTVLTRR318 pKa = 11.84SRR320 pKa = 11.84HH321 pKa = 3.94NRR323 pKa = 11.84AARR326 pKa = 11.84LSFLLTARR334 pKa = 11.84HH335 pKa = 5.62RR336 pKa = 11.84AEE338 pKa = 5.74EE339 pKa = 3.98IDD341 pKa = 3.37QCIDD345 pKa = 3.33MLDD348 pKa = 3.41ALAPEE353 pKa = 4.92IEE355 pKa = 4.3APTNIEE361 pKa = 3.99APKK364 pKa = 10.04GRR366 pKa = 11.84PVLACQPRR374 pKa = 11.84GG375 pKa = 3.37

Molecular weight: 40.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2883

0

2883

910118

26

2979

315.7

35.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.19 ± 0.045

1.003 ± 0.017

5.216 ± 0.038

6.069 ± 0.038

4.047 ± 0.033

6.918 ± 0.047

2.588 ± 0.025

6.804 ± 0.035

4.521 ± 0.037

10.817 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.432 ± 0.022

3.739 ± 0.032

4.527 ± 0.03

4.28 ± 0.033

5.85 ± 0.04

5.934 ± 0.031

5.301 ± 0.035

6.515 ± 0.036

1.344 ± 0.019

2.906 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski