Fusarium poae alternavirus 1
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA65|A0A1B4ZA65_9VIRU Uncharacterized protein OS=Fusarium poae alternavirus 1 OX=1849539 PE=4 SV=1
MM1 pKa = 7.41 SSAVNADD8 pKa = 3.33 NVPPKK13 pKa = 10.8 SIDD16 pKa = 3.32 GFVNNAYY23 pKa = 9.28 MEE25 pKa = 4.34 YY26 pKa = 10.42 EE27 pKa = 4.11 LEE29 pKa = 4.19 KK30 pKa = 11.03 LLAEE34 pKa = 4.94 LDD36 pKa = 3.95 VADD39 pKa = 5.34 PEE41 pKa = 4.39 PALYY45 pKa = 9.91 QPPAADD51 pKa = 3.18 TSVPDD56 pKa = 3.62 IEE58 pKa = 5.51 LPDD61 pKa = 3.74 VTSDD65 pKa = 4.44 PINDD69 pKa = 3.47 SRR71 pKa = 11.84 VGSDD75 pKa = 2.98 PVHH78 pKa = 6.31 NVRR81 pKa = 11.84 LAPPLPAEE89 pKa = 4.78 DD90 pKa = 3.61 GQGRR94 pKa = 11.84 VEE96 pKa = 4.17 SEE98 pKa = 4.18 AVFDD102 pKa = 4.17 VPLVRR107 pKa = 11.84 AEE109 pKa = 3.83 PAGDD113 pKa = 3.08 IDD115 pKa = 4.41 ARR117 pKa = 11.84 QPVTNNISSLDD128 pKa = 3.5 ASFQQHH134 pKa = 6.49 LGLARR139 pKa = 11.84 SANASARR146 pKa = 11.84 RR147 pKa = 11.84 AGTLAASIAYY157 pKa = 8.19 TLGADD162 pKa = 3.53 GTTVSEE168 pKa = 4.14 AAPLGLAVRR177 pKa = 11.84 IIAGEE182 pKa = 3.91 MAAMQGATASIQPSEE197 pKa = 4.39 SVLDD201 pKa = 4.38 VITTPIAHH209 pKa = 6.42 VGDD212 pKa = 4.68 AEE214 pKa = 4.82 DD215 pKa = 3.95 NNPDD219 pKa = 3.47 FFSVYY224 pKa = 10.51 LPAQLSAGEE233 pKa = 4.12 KK234 pKa = 9.45 SALVSLLVPGGPGAYY249 pKa = 5.93 TWRR252 pKa = 11.84 YY253 pKa = 9.61 RR254 pKa = 11.84 EE255 pKa = 3.99 PDD257 pKa = 3.57 DD258 pKa = 4.1 PRR260 pKa = 11.84 AAVMPAITRR269 pKa = 11.84 KK270 pKa = 9.83 LFAGGVEE277 pKa = 4.5 RR278 pKa = 11.84 ILAVTEE284 pKa = 4.05 RR285 pKa = 11.84 ADD287 pKa = 3.31 VLPAVGGAALTYY299 pKa = 10.94 ANLFGLEE306 pKa = 3.72 RR307 pKa = 11.84 YY308 pKa = 8.74 YY309 pKa = 10.8 RR310 pKa = 11.84 RR311 pKa = 11.84 HH312 pKa = 6.0 FGNAVFDD319 pKa = 4.21 AAWRR323 pKa = 11.84 TVYY326 pKa = 10.38 AASAVYY332 pKa = 9.96 VEE334 pKa = 4.34 PDD336 pKa = 3.05 IVEE339 pKa = 4.32 PCPLRR344 pKa = 11.84 PGDD347 pKa = 3.57 NFVVRR352 pKa = 11.84 RR353 pKa = 11.84 TTHH356 pKa = 6.29 QIDD359 pKa = 3.89 GLDD362 pKa = 3.75 LQDD365 pKa = 6.68 DD366 pKa = 4.14 MFTWLGGQHH375 pKa = 5.68 VPPRR379 pKa = 11.84 YY380 pKa = 9.26 RR381 pKa = 11.84 DD382 pKa = 3.83 FLPVGYY388 pKa = 10.36 EE389 pKa = 3.95 DD390 pKa = 4.15 FSKK393 pKa = 11.16 DD394 pKa = 2.89 RR395 pKa = 11.84 VLRR398 pKa = 11.84 RR399 pKa = 11.84 FNDD402 pKa = 3.76 LAWEE406 pKa = 4.5 LGRR409 pKa = 11.84 EE410 pKa = 4.14 NNEE413 pKa = 3.68 NLPARR418 pKa = 11.84 WKK420 pKa = 10.34 RR421 pKa = 11.84 AEE423 pKa = 4.2 PYY425 pKa = 8.48 ATGVTFVYY433 pKa = 9.19 MRR435 pKa = 11.84 PAEE438 pKa = 4.28 GDD440 pKa = 3.08 GDD442 pKa = 4.15 AEE444 pKa = 4.3 EE445 pKa = 5.35 DD446 pKa = 3.94 NIDD449 pKa = 4.54 FGWDD453 pKa = 3.26 DD454 pKa = 4.9 LLPFLAEE461 pKa = 3.79 LALPKK466 pKa = 10.5 DD467 pKa = 3.34 RR468 pKa = 11.84 LFFRR472 pKa = 11.84 TGYY475 pKa = 9.57 YY476 pKa = 9.86 HH477 pKa = 7.39 FSRR480 pKa = 11.84 LGLPDD485 pKa = 3.6 SLVEE489 pKa = 4.05 GDD491 pKa = 4.75 RR492 pKa = 11.84 DD493 pKa = 3.87 PVPPDD498 pKa = 2.86 IGRR501 pKa = 11.84 GHH503 pKa = 8.45 LIRR506 pKa = 11.84 NVYY509 pKa = 9.24 GAADD513 pKa = 4.2 FASMNLPGLRR523 pKa = 11.84 INEE526 pKa = 4.08 IVGLVAHH533 pKa = 6.64 FTRR536 pKa = 11.84 PAARR540 pKa = 11.84 TAIRR544 pKa = 11.84 EE545 pKa = 3.8 NHH547 pKa = 5.97 IRR549 pKa = 11.84 QQRR552 pKa = 11.84 KK553 pKa = 8.07 GRR555 pKa = 11.84 ARR557 pKa = 11.84 NYY559 pKa = 9.24 VAHH562 pKa = 6.78 LHH564 pKa = 6.06 LLNFYY569 pKa = 10.85 SYY571 pKa = 10.62 FRR573 pKa = 11.84 SNVWPSDD580 pKa = 3.43 LMQVSYY586 pKa = 10.18 PHH588 pKa = 7.0 APFLKK593 pKa = 10.31 RR594 pKa = 11.84 LAKK597 pKa = 9.9 PIRR600 pKa = 11.84 PFCWMDD606 pKa = 3.36 EE607 pKa = 4.48 LDD609 pKa = 3.7 PSAYY613 pKa = 9.39 PGQNWWVLEE622 pKa = 3.91 SAYY625 pKa = 10.64 LIRR628 pKa = 11.84 GTNNRR633 pKa = 11.84 TPFGGFLVAGTWEE646 pKa = 4.42 CDD648 pKa = 2.99 TRR650 pKa = 11.84 GVVDD654 pKa = 4.14 LGKK657 pKa = 10.32 RR658 pKa = 11.84 EE659 pKa = 3.86 NSLIAIEE666 pKa = 4.09 AAFRR670 pKa = 11.84 ASVNDD675 pKa = 3.73 GGQISASGRR684 pKa = 11.84 FGNTTFDD691 pKa = 4.17 IIRR694 pKa = 11.84 GANGMVNPMPFAPRR708 pKa = 11.84 IRR710 pKa = 11.84 TLRR713 pKa = 11.84 GSEE716 pKa = 4.23 SVDD719 pKa = 3.36 LAADD723 pKa = 3.82 GGVNTLQPGCNLSAGNVYY741 pKa = 10.56 SVV743 pKa = 3.29
Molecular weight: 81.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.929
IPC2_protein 4.927
IPC_protein 4.914
Toseland 4.8
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.029
Wikipedia 4.787
Rodwell 4.787
Grimsley 4.724
Solomon 4.902
Lehninger 4.851
Nozaki 5.016
DTASelect 5.207
Thurlkill 4.813
EMBOSS 4.813
Sillero 5.067
Patrickios 4.062
IPC_peptide 4.902
IPC2_peptide 5.067
IPC2.peptide.svr19 5.016
Protein with the highest isoelectric point:
>tr|A0A1B4ZA56|A0A1B4ZA56_9VIRU Uncharacterized protein OS=Fusarium poae alternavirus 1 OX=1849539 PE=4 SV=1
MM1 pKa = 6.81 WSARR5 pKa = 11.84 VVLSNASAASSWFKK19 pKa = 10.65 RR20 pKa = 11.84 RR21 pKa = 11.84 AEE23 pKa = 3.71 SRR25 pKa = 11.84 YY26 pKa = 9.77 DD27 pKa = 3.51 VKK29 pKa = 11.09 FCLPEE34 pKa = 5.8 RR35 pKa = 11.84 DD36 pKa = 3.67 VLGSISEE43 pKa = 4.1 QAKK46 pKa = 10.15 DD47 pKa = 3.77 VPSLSGSWAPEE58 pKa = 3.95 AFWVPTQQADD68 pKa = 3.86 GFPVFCLSSSHH79 pKa = 6.38 ACSEE83 pKa = 4.22 FVKK86 pKa = 10.85 LLADD90 pKa = 3.43 KK91 pKa = 10.76 GVRR94 pKa = 11.84 ATVATDD100 pKa = 3.23 HH101 pKa = 7.02 ASLHH105 pKa = 6.4 HH106 pKa = 6.72 SLFLCVTVTTVILPGMKK123 pKa = 9.78 RR124 pKa = 11.84 FNRR127 pKa = 11.84 DD128 pKa = 3.0 VVPEE132 pKa = 3.99 EE133 pKa = 3.67 LVNWVVEE140 pKa = 4.17 PWGLGCAHH148 pKa = 7.06 SDD150 pKa = 3.3 AYY152 pKa = 11.34 SKK154 pKa = 10.63 FVNHH158 pKa = 6.6 PLGRR162 pKa = 11.84 TEE164 pKa = 5.19 DD165 pKa = 3.32 VWGKK169 pKa = 10.34 LGAYY173 pKa = 8.66 PWEE176 pKa = 4.19 EE177 pKa = 4.41 LEE179 pKa = 4.85 NMLAKK184 pKa = 10.01 IVVSRR189 pKa = 11.84 IPVDD193 pKa = 3.66 DD194 pKa = 4.17 GVWRR198 pKa = 11.84 NLCGLLDD205 pKa = 4.25 AGDD208 pKa = 3.93 NYY210 pKa = 10.95 GALAEE215 pKa = 4.01 FDD217 pKa = 3.78 KK218 pKa = 11.36 LVTRR222 pKa = 11.84 GTKK225 pKa = 9.25 HH226 pKa = 6.01 KK227 pKa = 9.7 RR228 pKa = 11.84 RR229 pKa = 11.84 HH230 pKa = 5.3 YY231 pKa = 10.93 FDD233 pKa = 3.97 VCTLYY238 pKa = 10.7 TIASKK243 pKa = 10.53 VWSDD247 pKa = 3.3 SGQGPLFRR255 pKa = 11.84 RR256 pKa = 11.84 VAKK259 pKa = 10.43 LVLFDD264 pKa = 5.79 GITAQVGYY272 pKa = 10.33 EE273 pKa = 4.05 RR274 pKa = 11.84 LYY276 pKa = 9.69 LTCFWLFTTPYY287 pKa = 7.82 WQSICKK293 pKa = 9.93 FFLAEE298 pKa = 4.11 HH299 pKa = 6.67 LFFTTVDD306 pKa = 3.74 EE307 pKa = 4.35 YY308 pKa = 11.72 RR309 pKa = 11.84 DD310 pKa = 3.28 ICKK313 pKa = 10.31 EE314 pKa = 3.11 ITARR318 pKa = 11.84 VSSCWMFPRR327 pKa = 11.84 TNLQHH332 pKa = 6.37 IASAYY337 pKa = 9.33 FLNAEE342 pKa = 4.65 DD343 pKa = 3.88 LTGFADD349 pKa = 3.76 NQEE352 pKa = 4.27 SSGQVAVEE360 pKa = 3.94 VLEE363 pKa = 4.3 FALSEE368 pKa = 4.25 FEE370 pKa = 4.07 YY371 pKa = 10.51 TLPGDD376 pKa = 3.65 EE377 pKa = 4.26 GRR379 pKa = 11.84 SFDD382 pKa = 4.38 MYY384 pKa = 11.08 LKK386 pKa = 10.39 AYY388 pKa = 9.48 RR389 pKa = 11.84 DD390 pKa = 3.68 AVRR393 pKa = 11.84 SLLEE397 pKa = 3.44 PLYY400 pKa = 10.98 RR401 pKa = 11.84 EE402 pKa = 4.49 FGVKK406 pKa = 9.75 AQTYY410 pKa = 10.54 DD411 pKa = 3.7 EE412 pKa = 4.2 ILEE415 pKa = 4.3 TRR417 pKa = 11.84 TAWAAGGVAGRR428 pKa = 11.84 RR429 pKa = 11.84 ARR431 pKa = 11.84 DD432 pKa = 3.23 VLGAEE437 pKa = 4.24 RR438 pKa = 11.84 APPGSSKK445 pKa = 10.89 AYY447 pKa = 8.74 VMSMSRR453 pKa = 11.84 ADD455 pKa = 4.37 DD456 pKa = 4.5 FRR458 pKa = 11.84 MDD460 pKa = 3.03 WGDD463 pKa = 3.29 TRR465 pKa = 11.84 NEE467 pKa = 3.91 IANKK471 pKa = 8.41 MDD473 pKa = 3.08 EE474 pKa = 4.61 RR475 pKa = 11.84 GPPRR479 pKa = 11.84 CIQATDD485 pKa = 3.5 MRR487 pKa = 11.84 DD488 pKa = 3.42 QTAEE492 pKa = 4.02 TYY494 pKa = 10.33 VFKK497 pKa = 10.84 HH498 pKa = 6.22 FSNKK502 pKa = 8.68 YY503 pKa = 8.08 PRR505 pKa = 11.84 VGLDD509 pKa = 2.61 IGEE512 pKa = 4.57 SPTEE516 pKa = 3.57 AMARR520 pKa = 11.84 HH521 pKa = 5.49 AQLVGATRR529 pKa = 11.84 GSRR532 pKa = 11.84 VYY534 pKa = 10.86 KK535 pKa = 10.4 FDD537 pKa = 4.2 GRR539 pKa = 11.84 LLTAWDD545 pKa = 3.54 WQKK548 pKa = 10.73 WDD550 pKa = 3.58 HH551 pKa = 6.62 FYY553 pKa = 11.36 HH554 pKa = 6.61 NSEE557 pKa = 4.18 KK558 pKa = 11.04 VIVLQVMRR566 pKa = 11.84 EE567 pKa = 4.19 LTGHH571 pKa = 6.13 FVRR574 pKa = 11.84 PAVKK578 pKa = 10.42 AEE580 pKa = 3.89 MLRR583 pKa = 11.84 EE584 pKa = 3.96 LDD586 pKa = 4.31 DD587 pKa = 6.4 LIDD590 pKa = 3.2 KK591 pKa = 10.08 HH592 pKa = 7.95 SRR594 pKa = 11.84 MVYY597 pKa = 10.03 RR598 pKa = 11.84 SQAFADD604 pKa = 4.05 EE605 pKa = 4.87 YY606 pKa = 10.75 YY607 pKa = 11.19 GKK609 pKa = 10.62 LADD612 pKa = 4.05 EE613 pKa = 5.51 VIASSNGRR621 pKa = 11.84 AYY623 pKa = 10.84 RR624 pKa = 11.84 LDD626 pKa = 3.3 GRR628 pKa = 11.84 EE629 pKa = 4.17 GEE631 pKa = 4.2 VSIMIEE637 pKa = 4.27 KK638 pKa = 10.6 PNGQQSGRR646 pKa = 11.84 KK647 pKa = 5.09 TTLEE651 pKa = 3.95 ANTIVGTSRR660 pKa = 11.84 LLVRR664 pKa = 11.84 DD665 pKa = 3.84 AEE667 pKa = 4.43 LLGTRR672 pKa = 11.84 ASVKK676 pKa = 10.39 NRR678 pKa = 11.84 VALYY682 pKa = 10.18 SLNRR686 pKa = 11.84 ADD688 pKa = 5.76 DD689 pKa = 3.64 VAEE692 pKa = 3.79 VHH694 pKa = 6.39 ACYY697 pKa = 10.22 KK698 pKa = 10.41 RR699 pKa = 11.84 GVDD702 pKa = 4.43 AVNTMLAQGHH712 pKa = 5.87 RR713 pKa = 11.84 ANPKK717 pKa = 9.43 KK718 pKa = 10.29 QVAQWRR724 pKa = 11.84 SVVYY728 pKa = 10.12 LRR730 pKa = 11.84 ILYY733 pKa = 10.47 AGGSMRR739 pKa = 11.84 AFPPRR744 pKa = 11.84 AVYY747 pKa = 9.83 AAASGHH753 pKa = 6.05 PDD755 pKa = 2.89 KK756 pKa = 11.46 GAGSEE761 pKa = 4.24 SAFIDD766 pKa = 3.7 KK767 pKa = 10.69 LKK769 pKa = 10.71 SCSKK773 pKa = 10.71 GLDD776 pKa = 2.92 MWVRR780 pKa = 11.84 RR781 pKa = 11.84 GGFMRR786 pKa = 11.84 MAQALYY792 pKa = 10.75 ADD794 pKa = 3.99 VEE796 pKa = 4.53 RR797 pKa = 11.84 FFSKK801 pKa = 9.45 TRR803 pKa = 11.84 VWAIKK808 pKa = 9.7 GDD810 pKa = 3.37 KK811 pKa = 9.62 RR812 pKa = 11.84 TFTTKK817 pKa = 10.41 VIPVAALRR825 pKa = 11.84 AAPEE829 pKa = 3.98 NNGLGILPPGVYY841 pKa = 10.03 EE842 pKa = 3.6 YY843 pKa = 10.29 DD844 pKa = 3.7 YY845 pKa = 10.85 TVKK848 pKa = 10.57 CVTPDD853 pKa = 3.03 KK854 pKa = 10.85 YY855 pKa = 11.02 KK856 pKa = 10.89 AIADD860 pKa = 3.77 GWQRR864 pKa = 11.84 RR865 pKa = 11.84 IDD867 pKa = 3.62 EE868 pKa = 4.43 KK869 pKa = 11.05 VRR871 pKa = 11.84 AGIGPGIHH879 pKa = 6.91 DD880 pKa = 4.38 LEE882 pKa = 4.78 RR883 pKa = 11.84 RR884 pKa = 11.84 ARR886 pKa = 11.84 DD887 pKa = 3.2 WVFDD891 pKa = 3.6 NTKK894 pKa = 10.43 LVPTDD899 pKa = 3.84 KK900 pKa = 11.17 DD901 pKa = 3.17 MKK903 pKa = 10.31 RR904 pKa = 11.84 YY905 pKa = 8.55 KK906 pKa = 10.15 EE907 pKa = 3.53 KK908 pKa = 8.7 WAASRR913 pKa = 11.84 VHH915 pKa = 6.4 QDD917 pKa = 2.72 GTGDD921 pKa = 3.59 SKK923 pKa = 11.26 FLNRR927 pKa = 11.84 CKK929 pKa = 10.15 QVALAVKK936 pKa = 10.05 IVRR939 pKa = 11.84 VDD941 pKa = 3.04 KK942 pKa = 11.05 SDD944 pKa = 3.13 RR945 pKa = 11.84 SYY947 pKa = 10.97 IAKK950 pKa = 8.49 DD951 pKa = 3.14 TNRR954 pKa = 11.84 VVDD957 pKa = 4.14 RR958 pKa = 11.84 GLAALRR964 pKa = 11.84 GAIEE968 pKa = 4.0 QTQYY972 pKa = 10.88 MPGTHH977 pKa = 5.7 YY978 pKa = 11.28 AKK980 pKa = 10.44 DD981 pKa = 3.68 YY982 pKa = 10.33 LKK984 pKa = 10.54 SIRR987 pKa = 11.84 GFPGYY992 pKa = 11.31 GMTEE996 pKa = 4.15 HH997 pKa = 6.91 LWYY1000 pKa = 10.29 GYY1002 pKa = 11.01 GSILLASAKK1011 pKa = 9.94 EE1012 pKa = 3.89 RR1013 pKa = 11.84 GRR1015 pKa = 11.84 GEE1017 pKa = 3.87 WEE1019 pKa = 3.8 RR1020 pKa = 11.84 VLVLLANSSALGRR1033 pKa = 11.84 EE1034 pKa = 4.18 FLALSTSWTLEE1045 pKa = 3.47 ARR1047 pKa = 11.84 SAFLCGEE1054 pKa = 4.75 LGPVGGWDD1062 pKa = 3.66 KK1063 pKa = 11.23 LIPPSWIGWLNDD1075 pKa = 3.09 IVGLGLALEE1084 pKa = 4.37 LTIKK1088 pKa = 10.24 PALSRR1093 pKa = 11.84 CAWRR1097 pKa = 11.84 MFKK1100 pKa = 10.35 FRR1102 pKa = 11.84 ASLTKK1107 pKa = 8.99 EE1108 pKa = 3.5 AAVAFVNSYY1117 pKa = 11.03 PEE1119 pKa = 4.18 LCIHH1123 pKa = 7.31
Molecular weight: 126.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.02
IPC2_protein 8.17
IPC_protein 7.995
Toseland 8.053
ProMoST 8.565
Dawson 8.829
Bjellqvist 8.96
Wikipedia 8.873
Rodwell 8.902
Grimsley 8.185
Solomon 8.873
Lehninger 8.843
Nozaki 9.063
DTASelect 8.77
Thurlkill 8.843
EMBOSS 8.975
Sillero 9.077
Patrickios 4.52
IPC_peptide 8.858
IPC2_peptide 7.761
IPC2.peptide.svr19 7.852
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2622
743
1123
874.0
97.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.031 ± 0.629
1.106 ± 0.316
6.751 ± 0.296
5.53 ± 0.11
4.348 ± 0.107
8.162 ± 0.485
2.174 ± 0.182
3.318 ± 0.344
3.699 ± 1.066
9.039 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.869 ± 0.144
3.471 ± 0.584
5.263 ± 0.961
2.288 ± 0.07
8.314 ± 0.332
6.14 ± 0.239
4.729 ± 0.196
8.238 ± 0.406
1.945 ± 0.436
3.585 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here