Sphingopyxis sp. LPB0140

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; unclassified Sphingopyxis

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2350 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3J9A9|A0A1L3J9A9_9SPHN Aldo/keto reductase OS=Sphingopyxis sp. LPB0140 OX=1913578 GN=LPB140_01225 PE=4 SV=1
MM1 pKa = 7.53RR2 pKa = 11.84HH3 pKa = 6.14LAFDD7 pKa = 3.56NQKK10 pKa = 8.29TVISSSEE17 pKa = 3.83QARR20 pKa = 11.84KK21 pKa = 9.25LLKK24 pKa = 9.34RR25 pKa = 11.84AKK27 pKa = 8.39WLAGASILAASSTAMAAPSLVVNNVGTPTVVGTGQGKK64 pKa = 9.02RR65 pKa = 11.84AIWTNAGTVGSDD77 pKa = 3.32TIDD80 pKa = 3.31LVGVITTATLNHH92 pKa = 6.18TWSTTANRR100 pKa = 11.84PSITSVGPDD109 pKa = 3.78DD110 pKa = 5.92IFIEE114 pKa = 3.97WRR116 pKa = 11.84LYY118 pKa = 10.42RR119 pKa = 11.84AGTYY123 pKa = 10.58NITTNSGGVPVVADD137 pKa = 2.84VHH139 pKa = 6.12VQFNDD144 pKa = 2.69VDD146 pKa = 3.86GPNNEE151 pKa = 4.33RR152 pKa = 11.84IYY154 pKa = 11.23LPVCQGDD161 pKa = 3.83ISWVRR166 pKa = 11.84IDD168 pKa = 3.49ATATTGRR175 pKa = 11.84AFGAVAGQAEE185 pKa = 4.88TFSLIGDD192 pKa = 3.56QNYY195 pKa = 10.22NNQPVSGLEE204 pKa = 3.95ALWKK208 pKa = 10.51DD209 pKa = 2.99RR210 pKa = 11.84STFTMGRR217 pKa = 11.84TANSGFLIRR226 pKa = 11.84FDD228 pKa = 3.77NPTYY232 pKa = 10.88SAFDD236 pKa = 3.57TLDD239 pKa = 3.52FEE241 pKa = 5.23CADD244 pKa = 4.22FKK246 pKa = 11.52PPVTVNDD253 pKa = 3.84SKK255 pKa = 11.45EE256 pKa = 4.25GVPGTSTIVDD266 pKa = 3.27ILDD269 pKa = 3.9NDD271 pKa = 4.16SSATLNNNPTNNNSLKK287 pKa = 10.55ASEE290 pKa = 4.23FARR293 pKa = 11.84ASVNLVPPPSATGIITDD310 pKa = 3.69SFGDD314 pKa = 3.63VIGFTVPGEE323 pKa = 4.51GVWSYY328 pKa = 12.09SDD330 pKa = 3.21TTGQLTFTPDD340 pKa = 3.3PSFVGYY346 pKa = 10.81ASTVNYY352 pKa = 9.32TVDD355 pKa = 3.26NALGIQSNQATVTVWYY371 pKa = 9.12PGIGVTKK378 pKa = 10.66ASTFNDD384 pKa = 3.76LNGDD388 pKa = 3.61GYY390 pKa = 10.93GQVGEE395 pKa = 4.53TINYY399 pKa = 7.86IYY401 pKa = 9.95QVKK404 pKa = 10.36SYY406 pKa = 10.57GAEE409 pKa = 3.93PLRR412 pKa = 11.84NPSLTEE418 pKa = 3.81TTFTGAGTPPTPTYY432 pKa = 11.2VSGDD436 pKa = 3.87TNNDD440 pKa = 2.99GYY442 pKa = 10.71IGLTEE447 pKa = 3.83TWFYY451 pKa = 10.73SAVYY455 pKa = 10.3SLVAGDD461 pKa = 4.36LSGTGVNNSATASGNTAAGTVVNDD485 pKa = 3.86VSDD488 pKa = 3.85SANPADD494 pKa = 4.3GNNNTKK500 pKa = 10.45SGPGPGNNDD509 pKa = 2.87ATVTAVPRR517 pKa = 11.84APIAASNDD525 pKa = 3.88TQSGTITNDD534 pKa = 2.97GQANAFNVLSNDD546 pKa = 3.61SLKK549 pKa = 11.01GAAPTPSNVAITVTTPASHH568 pKa = 7.13AGVTLNTSTGQVSVAPGTPAGNYY591 pKa = 6.86TINYY595 pKa = 6.67QICEE599 pKa = 3.9IGNPTNCANAVATVNVSLRR618 pKa = 11.84PINADD623 pKa = 3.43DD624 pKa = 4.91DD625 pKa = 4.5SGPSVNSTNGSASLYY640 pKa = 10.37NVLSNDD646 pKa = 3.5TFNGGAATTPNVTINVTSPATPAFAGASVPSLNISTGVVSVPAGTPAGTYY696 pKa = 9.9NIGYY700 pKa = 7.32EE701 pKa = 4.17ICDD704 pKa = 3.39SAVPTNCEE712 pKa = 3.33AAIATIIVTPTPIAASNDD730 pKa = 3.13SGPAVNGAAGNTNAFNAFTNDD751 pKa = 3.37SFNGNPVNLSLVTATVTSPATPLSAGAPVPSLNTATGVVSVPAGTPAGSYY801 pKa = 8.84TIGYY805 pKa = 7.73RR806 pKa = 11.84ICEE809 pKa = 4.05NANPTNCANASITVQVAASPIAANNDD835 pKa = 2.96STPAVNSANGGSNLVNALGNDD856 pKa = 4.04SLNGAAPTASNVNITVTTPASNAGVTMNTATGQVSVAPGTPAGNYY901 pKa = 9.64SIGYY905 pKa = 7.13QICEE909 pKa = 4.1KK910 pKa = 10.7LNPSNCAIANVTIPVNVTPIAASADD935 pKa = 3.69TPPAQNGRR943 pKa = 11.84TGANDD948 pKa = 2.91IVNAFTNDD956 pKa = 3.54SLNGSPVNVADD967 pKa = 3.91IEE969 pKa = 4.32ATITAPATPLTAGAPVPVMDD989 pKa = 5.18PATGLVDD996 pKa = 4.08VPAGTPAGTYY1006 pKa = 8.56TIAYY1010 pKa = 7.19EE1011 pKa = 4.12ICEE1014 pKa = 4.19KK1015 pKa = 10.45TNPTNCANSSVTVIVSAAPITALNDD1040 pKa = 3.25NAGTVTTANGGANLINALGNDD1061 pKa = 4.16TLNGSPVALADD1072 pKa = 3.64VNLTVTAPASNAGVTLNTATGQVSVAPGTPAGNYY1106 pKa = 9.64SIGYY1110 pKa = 7.13QICEE1114 pKa = 4.17KK1115 pKa = 10.75LNPTNCQSATIAVVVDD1131 pKa = 4.03VTPLNASNDD1140 pKa = 3.23SPAPVNGANGGNDD1153 pKa = 3.64IINAFANDD1161 pKa = 4.2TLNGAPVNVADD1172 pKa = 4.75ISATITAPATPLTAGAPVPVMDD1194 pKa = 5.18PATGLVDD1201 pKa = 4.08VPAGTPAGTYY1211 pKa = 8.56TIAYY1215 pKa = 7.18EE1216 pKa = 3.77ICEE1219 pKa = 4.08NSNPTNCKK1227 pKa = 7.55TATVQVVVTAAPITADD1243 pKa = 3.68ADD1245 pKa = 3.64SPAPVNGATGGNDD1258 pKa = 3.53IINAFANDD1266 pKa = 4.17SLNGAPVNVADD1277 pKa = 4.75IEE1279 pKa = 4.43ATVTSPAMPLTAGAPVPVMDD1299 pKa = 5.18PATGLVDD1306 pKa = 4.08VPAGTPAGTYY1316 pKa = 8.56TIAYY1320 pKa = 7.25EE1321 pKa = 4.01ICEE1324 pKa = 4.09KK1325 pKa = 10.94LNPNNCASSTVNVVVTAAPITADD1348 pKa = 3.68ADD1350 pKa = 3.64SPAPVNGATGGNDD1363 pKa = 3.53IINAFANDD1371 pKa = 4.17SLNGAPVNVADD1382 pKa = 4.75IEE1384 pKa = 4.43ATVTSPATPLTAGAPVPVMDD1404 pKa = 5.18PATGLVDD1411 pKa = 3.83VPVGTPAGTYY1421 pKa = 8.32TIAYY1425 pKa = 7.25EE1426 pKa = 4.01ICEE1429 pKa = 4.09KK1430 pKa = 10.94LNPNNCATNNVTVVVTAAPIAASNDD1455 pKa = 3.02AVGPVPGISGGSNLINALNNDD1476 pKa = 4.15MLNGASVAIADD1487 pKa = 3.74INVTVTSPASHH1498 pKa = 6.82AGVTLDD1504 pKa = 4.06PATGMVSVAPNTPAGTYY1521 pKa = 9.0TIGYY1525 pKa = 7.18QICEE1529 pKa = 4.21KK1530 pKa = 10.84LNPTNCANAIISVTVDD1546 pKa = 3.73AAPIMADD1553 pKa = 3.01VDD1555 pKa = 4.0SPAPVNGANGGNDD1568 pKa = 3.64IINAFANDD1576 pKa = 4.17SLNGAPVNVADD1587 pKa = 4.46IDD1589 pKa = 3.98ATITTPATPATPGAPVPVMDD1609 pKa = 5.18PATGLVDD1616 pKa = 3.54VSVGTPAGTYY1626 pKa = 8.32TIAYY1630 pKa = 7.51EE1631 pKa = 4.19ICQKK1635 pKa = 10.92LNPTNCASSNVTIVVEE1651 pKa = 4.14APEE1654 pKa = 3.83ILAANDD1660 pKa = 2.82VPAPVRR1666 pKa = 11.84SGVGNPNAINAFANDD1681 pKa = 3.95ILNGSPVDD1689 pKa = 3.83VNDD1692 pKa = 3.47INVTILTPAAHH1703 pKa = 6.7AGVVLDD1709 pKa = 4.1PATGIVSVTANVPDD1723 pKa = 3.48GTYY1726 pKa = 9.72IIEE1729 pKa = 4.03YY1730 pKa = 9.15QICEE1734 pKa = 4.25KK1735 pKa = 10.9LNPTNCKK1742 pKa = 7.83TATVTVVVEE1751 pKa = 4.28PPVSSVTGTVFTDD1764 pKa = 3.34VNGDD1768 pKa = 3.51GVLGPDD1774 pKa = 3.43EE1775 pKa = 4.35PRR1777 pKa = 11.84RR1778 pKa = 11.84AGWIVEE1784 pKa = 3.85IMKK1787 pKa = 10.62DD1788 pKa = 3.65GVVVATTTTDD1798 pKa = 2.84ANGDD1802 pKa = 3.71YY1803 pKa = 10.57RR1804 pKa = 11.84VDD1806 pKa = 3.7GLLSGPGYY1814 pKa = 10.57DD1815 pKa = 2.52IVFRR1819 pKa = 11.84NPEE1822 pKa = 3.94NNVVYY1827 pKa = 10.7DD1828 pKa = 3.99KK1829 pKa = 11.33IEE1831 pKa = 3.99GVNLVNNTVVIDD1843 pKa = 3.67QNQPIDD1849 pKa = 3.99PSGVIYY1855 pKa = 10.49DD1856 pKa = 4.32SITRR1860 pKa = 11.84NPISGVTVRR1869 pKa = 11.84LLGPDD1874 pKa = 4.09GNPLPSICFVDD1885 pKa = 4.83ASQASQTTGASGEE1898 pKa = 3.99YY1899 pKa = 9.91RR1900 pKa = 11.84FDD1902 pKa = 4.93IIPGAAPQCPASEE1915 pKa = 4.17TVYY1918 pKa = 9.94TIQVTPPVGFADD1930 pKa = 4.65GSTVLVPLPGPFDD1943 pKa = 4.12PSGLPAPVRR1952 pKa = 11.84ISPDD1956 pKa = 2.86ATPPQGSDD1964 pKa = 3.06TPFYY1968 pKa = 10.95LSFRR1972 pKa = 11.84LQPGDD1977 pKa = 3.53PDD1979 pKa = 3.65VVNNHH1984 pKa = 6.21IALDD1988 pKa = 3.79PFLNRR1993 pKa = 11.84TPLVVTKK2000 pKa = 9.67TSIKK2004 pKa = 10.17RR2005 pKa = 11.84SASTGDD2011 pKa = 3.5LVPYY2015 pKa = 9.85EE2016 pKa = 3.69ITVRR2020 pKa = 11.84NTEE2023 pKa = 3.71NAQRR2027 pKa = 11.84AGVDD2031 pKa = 3.55VVDD2034 pKa = 4.53ILPAGMKK2041 pKa = 9.94YY2042 pKa = 10.69VLGTASVDD2050 pKa = 3.49GVANEE2055 pKa = 5.13PIATNNNRR2063 pKa = 11.84EE2064 pKa = 4.18LRR2066 pKa = 11.84WTGQVIPANGSVRR2079 pKa = 11.84YY2080 pKa = 9.8NLTLVVGAGVTGGEE2094 pKa = 4.27KK2095 pKa = 10.79VNTGLAQNAADD2106 pKa = 5.45FSAISNRR2113 pKa = 11.84GTAVVAIVPSAVFDD2127 pKa = 4.06CSEE2130 pKa = 3.96LLGKK2134 pKa = 10.38VFEE2137 pKa = 4.63DD2138 pKa = 3.88RR2139 pKa = 11.84NRR2141 pKa = 11.84NGYY2144 pKa = 8.45QDD2146 pKa = 3.64EE2147 pKa = 4.53NEE2149 pKa = 3.99PGIAGVRR2156 pKa = 11.84LATVNGQLITTDD2168 pKa = 2.82EE2169 pKa = 4.39FGRR2172 pKa = 11.84YY2173 pKa = 8.91HH2174 pKa = 6.79IACAAVPDD2182 pKa = 4.07ARR2184 pKa = 11.84IGSNFVLKK2192 pKa = 10.67VDD2194 pKa = 3.83TRR2196 pKa = 11.84TLPIGWEE2203 pKa = 4.13VTFDD2207 pKa = 3.5NPKK2210 pKa = 10.32SIRR2213 pKa = 11.84LTRR2216 pKa = 11.84GKK2218 pKa = 10.41FGEE2221 pKa = 4.42LNFGVAPRR2229 pKa = 11.84EE2230 pKa = 3.87EE2231 pKa = 4.28GAPTNSNGKK2240 pKa = 10.14GEE2242 pKa = 4.05

Molecular weight: 227.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3JAU6|A0A1L3JAU6_9SPHN Alcohol dehydrogenase OS=Sphingopyxis sp. LPB0140 OX=1913578 GN=LPB140_04890 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.54LNLGLFSLLILAMLAALFAGKK23 pKa = 9.43IWVPVDD29 pKa = 3.67EE30 pKa = 4.87IFGGGRR36 pKa = 11.84GALIIAEE43 pKa = 4.28LRR45 pKa = 11.84TPRR48 pKa = 11.84IILGVIIGGALGLSGAVMQGYY69 pKa = 9.33LRR71 pKa = 11.84NPLADD76 pKa = 4.25PGLFGVSSGAALGAVIAIFWGLAGEE101 pKa = 4.73IWALPLFALIGAGGSMALLGMLAGRR126 pKa = 11.84SGSLILITLGGVMLSTLTGSLTALVISLAPTPFATSQIITWLMGALSDD174 pKa = 4.68RR175 pKa = 11.84SWSDD179 pKa = 3.05VYY181 pKa = 10.72IAAPLIFIGMMILFATARR199 pKa = 11.84ALDD202 pKa = 3.93VLTLGDD208 pKa = 3.65VVARR212 pKa = 11.84SMGVDD217 pKa = 3.28MVRR220 pKa = 11.84LQWQVILGTGICVGAAVAAAGVIGFVGLMVPHH252 pKa = 7.89IIRR255 pKa = 11.84PFVGHH260 pKa = 6.77RR261 pKa = 11.84PSALLLPSALAGAILIISSDD281 pKa = 3.34ALVRR285 pKa = 11.84ILPTVSEE292 pKa = 4.36LRR294 pKa = 11.84LGIAMTMLGTPFFLFLLFKK313 pKa = 9.51MRR315 pKa = 11.84RR316 pKa = 11.84GIMM319 pKa = 3.43

Molecular weight: 33.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2350

0

2350

760127

53

3009

323.5

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.665 ± 0.055

0.874 ± 0.017

6.091 ± 0.043

5.538 ± 0.049

3.971 ± 0.033

8.107 ± 0.045

2.083 ± 0.029

7.347 ± 0.048

5.132 ± 0.044

9.037 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.008 ± 0.03

4.426 ± 0.047

4.445 ± 0.032

3.607 ± 0.032

5.177 ± 0.044

6.03 ± 0.046

4.555 ± 0.045

5.896 ± 0.057

1.302 ± 0.019

2.709 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski