Amino acid dipepetide frequency for Paenibacillus phage Scottie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.168AlaAla: 8.168 ± 0.677
0.992AlaCys: 0.992 ± 0.191
5.601AlaAsp: 5.601 ± 0.565
7.001AlaGlu: 7.001 ± 0.641
2.567AlaPhe: 2.567 ± 0.309
5.076AlaGly: 5.076 ± 0.767
1.284AlaHis: 1.284 ± 0.253
4.142AlaIle: 4.142 ± 0.472
5.893AlaLys: 5.893 ± 0.59
7.41AlaLeu: 7.41 ± 0.627
1.692AlaMet: 1.692 ± 0.329
3.909AlaAsn: 3.909 ± 0.482
2.567AlaPro: 2.567 ± 0.37
2.042AlaGln: 2.042 ± 0.299
4.434AlaArg: 4.434 ± 0.506
4.434AlaSer: 4.434 ± 0.505
3.559AlaThr: 3.559 ± 0.532
5.834AlaVal: 5.834 ± 0.653
1.167AlaTrp: 1.167 ± 0.324
3.151AlaTyr: 3.151 ± 0.474
0.0AlaXaa: 0.0 ± 0.0
Cys
0.817CysAla: 0.817 ± 0.208
0.058CysCys: 0.058 ± 0.058
0.525CysAsp: 0.525 ± 0.182
0.7CysGlu: 0.7 ± 0.181
0.583CysPhe: 0.583 ± 0.191
1.167CysGly: 1.167 ± 0.263
0.233CysHis: 0.233 ± 0.107
0.35CysIle: 0.35 ± 0.129
0.7CysLys: 0.7 ± 0.201
0.817CysLeu: 0.817 ± 0.218
0.175CysMet: 0.175 ± 0.096
0.175CysAsn: 0.175 ± 0.082
0.583CysPro: 0.583 ± 0.188
0.35CysGln: 0.35 ± 0.17
1.342CysArg: 1.342 ± 0.32
0.467CysSer: 0.467 ± 0.147
0.408CysThr: 0.408 ± 0.14
0.35CysVal: 0.35 ± 0.148
0.175CysTrp: 0.175 ± 0.084
0.35CysTyr: 0.35 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
5.076AspAla: 5.076 ± 0.621
0.642AspCys: 0.642 ± 0.207
5.893AspAsp: 5.893 ± 0.555
6.476AspGlu: 6.476 ± 0.544
3.559AspPhe: 3.559 ± 0.44
4.201AspGly: 4.201 ± 0.542
1.342AspHis: 1.342 ± 0.216
5.484AspIle: 5.484 ± 0.463
2.8AspLys: 2.8 ± 0.352
4.959AspLeu: 4.959 ± 0.459
1.867AspMet: 1.867 ± 0.268
2.275AspAsn: 2.275 ± 0.392
2.334AspPro: 2.334 ± 0.331
1.517AspGln: 1.517 ± 0.358
3.501AspArg: 3.501 ± 0.445
3.676AspSer: 3.676 ± 0.429
3.209AspThr: 3.209 ± 0.334
5.484AspVal: 5.484 ± 0.578
1.459AspTrp: 1.459 ± 0.278
2.625AspTyr: 2.625 ± 0.327
0.0AspXaa: 0.0 ± 0.0
Glu
5.426GluAla: 5.426 ± 0.528
0.758GluCys: 0.758 ± 0.213
4.084GluAsp: 4.084 ± 0.477
6.826GluGlu: 6.826 ± 0.69
3.267GluPhe: 3.267 ± 0.494
4.084GluGly: 4.084 ± 0.581
1.4GluHis: 1.4 ± 0.306
4.609GluIle: 4.609 ± 0.535
6.709GluLys: 6.709 ± 0.686
9.102GluLeu: 9.102 ± 0.778
2.8GluMet: 2.8 ± 0.41
2.625GluAsn: 2.625 ± 0.326
2.8GluPro: 2.8 ± 0.39
2.742GluGln: 2.742 ± 0.413
5.426GluArg: 5.426 ± 0.585
3.851GluSer: 3.851 ± 0.425
4.259GluThr: 4.259 ± 0.363
5.309GluVal: 5.309 ± 0.669
1.225GluTrp: 1.225 ± 0.326
3.734GluTyr: 3.734 ± 0.472
0.0GluXaa: 0.0 ± 0.0
Phe
3.034PheAla: 3.034 ± 0.489
0.35PheCys: 0.35 ± 0.145
2.859PheAsp: 2.859 ± 0.395
2.742PheGlu: 2.742 ± 0.472
1.167PhePhe: 1.167 ± 0.331
2.509PheGly: 2.509 ± 0.341
0.758PheHis: 0.758 ± 0.223
2.334PheIle: 2.334 ± 0.32
2.217PheLys: 2.217 ± 0.345
2.1PheLeu: 2.1 ± 0.315
1.109PheMet: 1.109 ± 0.282
1.925PheAsn: 1.925 ± 0.396
1.167PhePro: 1.167 ± 0.286
0.875PheGln: 0.875 ± 0.182
2.334PheArg: 2.334 ± 0.357
1.925PheSer: 1.925 ± 0.329
2.567PheThr: 2.567 ± 0.344
2.684PheVal: 2.684 ± 0.366
0.35PheTrp: 0.35 ± 0.125
1.575PheTyr: 1.575 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
3.967GlyAla: 3.967 ± 0.571
0.525GlyCys: 0.525 ± 0.197
4.784GlyAsp: 4.784 ± 0.538
4.667GlyGlu: 4.667 ± 0.587
2.45GlyPhe: 2.45 ± 0.345
5.076GlyGly: 5.076 ± 0.657
0.758GlyHis: 0.758 ± 0.196
5.134GlyIle: 5.134 ± 0.542
5.893GlyLys: 5.893 ± 0.512
5.426GlyLeu: 5.426 ± 0.54
1.517GlyMet: 1.517 ± 0.289
2.684GlyAsn: 2.684 ± 0.412
1.575GlyPro: 1.575 ± 0.304
1.925GlyGln: 1.925 ± 0.294
4.376GlyArg: 4.376 ± 0.53
2.567GlySer: 2.567 ± 0.398
3.909GlyThr: 3.909 ± 0.445
4.726GlyVal: 4.726 ± 0.487
1.05GlyTrp: 1.05 ± 0.229
2.509GlyTyr: 2.509 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.692HisAla: 1.692 ± 0.333
0.233HisCys: 0.233 ± 0.116
1.459HisAsp: 1.459 ± 0.275
1.575HisGlu: 1.575 ± 0.272
0.583HisPhe: 0.583 ± 0.183
1.284HisGly: 1.284 ± 0.248
0.292HisHis: 0.292 ± 0.129
0.875HisIle: 0.875 ± 0.208
0.875HisLys: 0.875 ± 0.195
0.992HisLeu: 0.992 ± 0.312
0.292HisMet: 0.292 ± 0.122
0.583HisAsn: 0.583 ± 0.186
0.817HisPro: 0.817 ± 0.189
0.7HisGln: 0.7 ± 0.197
1.109HisArg: 1.109 ± 0.317
0.933HisSer: 0.933 ± 0.234
0.758HisThr: 0.758 ± 0.247
1.225HisVal: 1.225 ± 0.291
0.175HisTrp: 0.175 ± 0.103
0.817HisTyr: 0.817 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.659IleAla: 5.659 ± 0.611
1.05IleCys: 1.05 ± 0.303
5.484IleAsp: 5.484 ± 0.535
4.376IleGlu: 4.376 ± 0.382
1.517IlePhe: 1.517 ± 0.311
4.026IleGly: 4.026 ± 0.485
1.05IleHis: 1.05 ± 0.246
3.442IleIle: 3.442 ± 0.376
4.609IleLys: 4.609 ± 0.525
3.851IleLeu: 3.851 ± 0.478
1.634IleMet: 1.634 ± 0.381
2.684IleAsn: 2.684 ± 0.399
2.684IlePro: 2.684 ± 0.463
1.575IleGln: 1.575 ± 0.269
4.551IleArg: 4.551 ± 0.446
2.859IleSer: 2.859 ± 0.463
3.384IleThr: 3.384 ± 0.465
4.259IleVal: 4.259 ± 0.557
0.642IleTrp: 0.642 ± 0.186
2.625IleTyr: 2.625 ± 0.428
0.0IleXaa: 0.0 ± 0.0
Lys
5.659LysAla: 5.659 ± 0.44
0.583LysCys: 0.583 ± 0.211
4.317LysAsp: 4.317 ± 0.452
4.784LysGlu: 4.784 ± 0.7
1.984LysPhe: 1.984 ± 0.344
4.201LysGly: 4.201 ± 0.543
1.4LysHis: 1.4 ± 0.322
4.901LysIle: 4.901 ± 0.442
5.368LysLys: 5.368 ± 0.639
5.834LysLeu: 5.834 ± 0.674
1.575LysMet: 1.575 ± 0.355
2.042LysAsn: 2.042 ± 0.318
2.392LysPro: 2.392 ± 0.34
2.334LysGln: 2.334 ± 0.38
5.776LysArg: 5.776 ± 0.638
3.734LysSer: 3.734 ± 0.468
4.259LysThr: 4.259 ± 0.451
4.784LysVal: 4.784 ± 0.568
1.4LysTrp: 1.4 ± 0.282
3.501LysTyr: 3.501 ± 0.509
0.0LysXaa: 0.0 ± 0.0
Leu
7.701LeuAla: 7.701 ± 0.65
0.7LeuCys: 0.7 ± 0.198
6.184LeuAsp: 6.184 ± 0.529
5.543LeuGlu: 5.543 ± 0.518
2.742LeuPhe: 2.742 ± 0.326
4.551LeuGly: 4.551 ± 0.465
1.634LeuHis: 1.634 ± 0.28
4.784LeuIle: 4.784 ± 0.42
4.901LeuLys: 4.901 ± 0.601
5.776LeuLeu: 5.776 ± 0.601
1.809LeuMet: 1.809 ± 0.285
2.392LeuAsn: 2.392 ± 0.392
3.326LeuPro: 3.326 ± 0.427
2.392LeuGln: 2.392 ± 0.348
6.359LeuArg: 6.359 ± 0.713
5.718LeuSer: 5.718 ± 0.504
5.834LeuThr: 5.834 ± 0.488
4.317LeuVal: 4.317 ± 0.461
0.642LeuTrp: 0.642 ± 0.234
3.209LeuTyr: 3.209 ± 0.418
0.0LeuXaa: 0.0 ± 0.0
Met
2.159MetAla: 2.159 ± 0.332
0.175MetCys: 0.175 ± 0.091
1.342MetAsp: 1.342 ± 0.268
1.575MetGlu: 1.575 ± 0.304
0.758MetPhe: 0.758 ± 0.173
1.284MetGly: 1.284 ± 0.253
0.408MetHis: 0.408 ± 0.138
1.05MetIle: 1.05 ± 0.238
1.692MetLys: 1.692 ± 0.301
2.042MetLeu: 2.042 ± 0.366
0.525MetMet: 0.525 ± 0.152
1.459MetAsn: 1.459 ± 0.264
1.225MetPro: 1.225 ± 0.264
0.817MetGln: 0.817 ± 0.206
2.509MetArg: 2.509 ± 0.346
1.984MetSer: 1.984 ± 0.271
1.634MetThr: 1.634 ± 0.291
1.05MetVal: 1.05 ± 0.246
0.292MetTrp: 0.292 ± 0.127
0.875MetTyr: 0.875 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
2.859AsnAla: 2.859 ± 0.414
0.467AsnCys: 0.467 ± 0.174
2.1AsnAsp: 2.1 ± 0.38
4.084AsnGlu: 4.084 ± 0.385
1.109AsnPhe: 1.109 ± 0.252
3.851AsnGly: 3.851 ± 0.459
0.583AsnHis: 0.583 ± 0.147
1.4AsnIle: 1.4 ± 0.27
1.984AsnLys: 1.984 ± 0.316
2.975AsnLeu: 2.975 ± 0.415
0.933AsnMet: 0.933 ± 0.246
1.575AsnAsn: 1.575 ± 0.296
2.275AsnPro: 2.275 ± 0.458
0.875AsnGln: 0.875 ± 0.262
2.859AsnArg: 2.859 ± 0.539
1.925AsnSer: 1.925 ± 0.396
1.809AsnThr: 1.809 ± 0.268
2.859AsnVal: 2.859 ± 0.378
0.7AsnTrp: 0.7 ± 0.206
1.225AsnTyr: 1.225 ± 0.279
0.0AsnXaa: 0.0 ± 0.0
Pro
2.859ProAla: 2.859 ± 0.453
0.233ProCys: 0.233 ± 0.109
2.859ProAsp: 2.859 ± 0.331
3.559ProGlu: 3.559 ± 0.401
1.05ProPhe: 1.05 ± 0.25
2.217ProGly: 2.217 ± 0.437
0.467ProHis: 0.467 ± 0.138
2.45ProIle: 2.45 ± 0.327
2.45ProLys: 2.45 ± 0.32
2.742ProLeu: 2.742 ± 0.46
1.225ProMet: 1.225 ± 0.229
1.867ProAsn: 1.867 ± 0.308
0.933ProPro: 0.933 ± 0.273
0.992ProGln: 0.992 ± 0.236
1.867ProArg: 1.867 ± 0.36
1.575ProSer: 1.575 ± 0.257
2.509ProThr: 2.509 ± 0.372
1.984ProVal: 1.984 ± 0.35
0.35ProTrp: 0.35 ± 0.146
1.75ProTyr: 1.75 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
2.859GlnAla: 2.859 ± 0.397
0.292GlnCys: 0.292 ± 0.138
1.284GlnAsp: 1.284 ± 0.356
2.042GlnGlu: 2.042 ± 0.332
0.992GlnPhe: 0.992 ± 0.252
1.284GlnGly: 1.284 ± 0.34
0.408GlnHis: 0.408 ± 0.154
1.925GlnIle: 1.925 ± 0.279
2.159GlnLys: 2.159 ± 0.276
2.975GlnLeu: 2.975 ± 0.351
0.817GlnMet: 0.817 ± 0.225
1.4GlnAsn: 1.4 ± 0.266
0.583GlnPro: 0.583 ± 0.17
0.992GlnGln: 0.992 ± 0.258
2.45GlnArg: 2.45 ± 0.362
1.167GlnSer: 1.167 ± 0.277
2.509GlnThr: 2.509 ± 0.481
1.634GlnVal: 1.634 ± 0.344
0.583GlnTrp: 0.583 ± 0.173
1.284GlnTyr: 1.284 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
4.551ArgAla: 4.551 ± 0.602
0.7ArgCys: 0.7 ± 0.227
3.851ArgAsp: 3.851 ± 0.471
6.768ArgGlu: 6.768 ± 0.764
2.392ArgPhe: 2.392 ± 0.494
3.501ArgGly: 3.501 ± 0.458
1.05ArgHis: 1.05 ± 0.247
4.259ArgIle: 4.259 ± 0.478
6.534ArgLys: 6.534 ± 0.605
6.359ArgLeu: 6.359 ± 0.668
1.984ArgMet: 1.984 ± 0.322
3.967ArgAsn: 3.967 ± 0.553
1.984ArgPro: 1.984 ± 0.327
2.45ArgGln: 2.45 ± 0.339
4.259ArgArg: 4.259 ± 0.686
2.509ArgSer: 2.509 ± 0.34
2.975ArgThr: 2.975 ± 0.428
3.617ArgVal: 3.617 ± 0.537
0.933ArgTrp: 0.933 ± 0.254
2.217ArgTyr: 2.217 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
4.376SerAla: 4.376 ± 0.526
0.583SerCys: 0.583 ± 0.214
3.092SerAsp: 3.092 ± 0.348
4.084SerGlu: 4.084 ± 0.47
2.334SerPhe: 2.334 ± 0.327
4.201SerGly: 4.201 ± 0.446
0.992SerHis: 0.992 ± 0.253
2.975SerIle: 2.975 ± 0.405
3.792SerLys: 3.792 ± 0.445
3.326SerLeu: 3.326 ± 0.418
1.225SerMet: 1.225 ± 0.234
1.634SerAsn: 1.634 ± 0.323
2.275SerPro: 2.275 ± 0.374
1.517SerGln: 1.517 ± 0.355
2.859SerArg: 2.859 ± 0.404
3.209SerSer: 3.209 ± 0.406
2.334SerThr: 2.334 ± 0.384
3.909SerVal: 3.909 ± 0.511
0.817SerTrp: 0.817 ± 0.234
2.45SerTyr: 2.45 ± 0.366
0.0SerXaa: 0.0 ± 0.0
Thr
4.609ThrAla: 4.609 ± 0.675
0.642ThrCys: 0.642 ± 0.23
4.084ThrAsp: 4.084 ± 0.448
4.434ThrGlu: 4.434 ± 0.449
2.567ThrPhe: 2.567 ± 0.386
4.959ThrGly: 4.959 ± 0.499
0.875ThrHis: 0.875 ± 0.193
3.209ThrIle: 3.209 ± 0.449
3.734ThrLys: 3.734 ± 0.603
3.734ThrLeu: 3.734 ± 0.363
1.109ThrMet: 1.109 ± 0.288
1.459ThrAsn: 1.459 ± 0.271
2.275ThrPro: 2.275 ± 0.369
2.042ThrGln: 2.042 ± 0.347
2.917ThrArg: 2.917 ± 0.404
2.684ThrSer: 2.684 ± 0.342
2.159ThrThr: 2.159 ± 0.359
4.026ThrVal: 4.026 ± 0.469
0.992ThrTrp: 0.992 ± 0.225
2.859ThrTyr: 2.859 ± 0.402
0.0ThrXaa: 0.0 ± 0.0
Val
5.193ValAla: 5.193 ± 0.595
0.933ValCys: 0.933 ± 0.274
4.142ValAsp: 4.142 ± 0.421
5.484ValGlu: 5.484 ± 0.515
2.859ValPhe: 2.859 ± 0.305
3.851ValGly: 3.851 ± 0.525
1.225ValHis: 1.225 ± 0.258
4.842ValIle: 4.842 ± 0.529
4.726ValLys: 4.726 ± 0.601
5.776ValLeu: 5.776 ± 0.578
1.459ValMet: 1.459 ± 0.331
2.45ValAsn: 2.45 ± 0.385
2.509ValPro: 2.509 ± 0.376
1.809ValGln: 1.809 ± 0.258
4.259ValArg: 4.259 ± 0.478
3.734ValSer: 3.734 ± 0.392
4.026ValThr: 4.026 ± 0.489
4.259ValVal: 4.259 ± 0.498
0.817ValTrp: 0.817 ± 0.194
2.684ValTyr: 2.684 ± 0.354
0.0ValXaa: 0.0 ± 0.0
Trp
0.933TrpAla: 0.933 ± 0.206
0.0TrpCys: 0.0 ± 0.0
1.225TrpAsp: 1.225 ± 0.333
1.4TrpGlu: 1.4 ± 0.265
0.467TrpPhe: 0.467 ± 0.159
0.642TrpGly: 0.642 ± 0.203
0.525TrpHis: 0.525 ± 0.174
0.875TrpIle: 0.875 ± 0.216
0.875TrpLys: 0.875 ± 0.206
1.167TrpLeu: 1.167 ± 0.258
0.35TrpMet: 0.35 ± 0.151
0.525TrpAsn: 0.525 ± 0.147
0.292TrpPro: 0.292 ± 0.133
0.758TrpGln: 0.758 ± 0.201
0.7TrpArg: 0.7 ± 0.182
0.7TrpSer: 0.7 ± 0.235
1.05TrpThr: 1.05 ± 0.214
1.225TrpVal: 1.225 ± 0.299
0.117TrpTrp: 0.117 ± 0.081
0.642TrpTyr: 0.642 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.326TyrAla: 3.326 ± 0.367
0.408TyrCys: 0.408 ± 0.153
3.209TyrAsp: 3.209 ± 0.504
2.917TyrGlu: 2.917 ± 0.346
1.634TyrPhe: 1.634 ± 0.273
3.559TyrGly: 3.559 ± 0.451
0.583TyrHis: 0.583 ± 0.208
2.917TyrIle: 2.917 ± 0.428
2.917TyrLys: 2.917 ± 0.342
3.209TyrLeu: 3.209 ± 0.472
0.7TyrMet: 0.7 ± 0.203
0.817TyrAsn: 0.817 ± 0.2
1.342TyrPro: 1.342 ± 0.305
0.933TyrGln: 0.933 ± 0.21
2.975TyrArg: 2.975 ± 0.445
2.334TyrSer: 2.334 ± 0.4
2.217TyrThr: 2.217 ± 0.398
3.501TyrVal: 3.501 ± 0.474
0.583TyrTrp: 0.583 ± 0.179
1.984TyrTyr: 1.984 ± 0.418
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (17141 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski