Alteromonas aestuariivivens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3306 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8M4I8|A0A3D8M4I8_9ALTE Na+/H+ antiporter subunit C OS=Alteromonas aestuariivivens OX=1938339 GN=DXV75_13275 PE=4 SV=1
NN1 pKa = 7.69GSLTLNSDD9 pKa = 3.41GTFTYY14 pKa = 10.66VHH16 pKa = 7.38DD17 pKa = 4.7GGEE20 pKa = 4.29TTSDD24 pKa = 3.1SFTYY28 pKa = 9.8QANDD32 pKa = 3.42GSSTSNTVTVSISITADD49 pKa = 2.57NDD51 pKa = 3.57APVAVADD58 pKa = 4.28SYY60 pKa = 11.9SVTEE64 pKa = 4.23GGTLNGTSVLSNDD77 pKa = 3.22TDD79 pKa = 3.85AEE81 pKa = 4.42SDD83 pKa = 3.66SLTAVQVSSPANGSLTLNSDD103 pKa = 3.41GTFTYY108 pKa = 10.66VHH110 pKa = 7.38DD111 pKa = 4.7GGEE114 pKa = 4.29TTSDD118 pKa = 3.1SFTYY122 pKa = 9.8QANDD126 pKa = 3.35GSSNSNTVTVSISITADD143 pKa = 2.63NDD145 pKa = 3.53APVAKK150 pKa = 9.48DD151 pKa = 3.29DD152 pKa = 4.13SVAVDD157 pKa = 4.21EE158 pKa = 5.74DD159 pKa = 3.74STDD162 pKa = 3.29NVIPVLDD169 pKa = 3.97NDD171 pKa = 4.05IDD173 pKa = 4.09PEE175 pKa = 4.48GDD177 pKa = 3.32EE178 pKa = 4.36LTITQATASEE188 pKa = 4.65GSVSINSDD196 pKa = 2.9GTLSYY201 pKa = 10.56TPEE204 pKa = 3.84EE205 pKa = 4.14NATDD209 pKa = 3.78SVTIRR214 pKa = 11.84YY215 pKa = 7.11TISDD219 pKa = 4.06ANGLTDD225 pKa = 3.58TASVTVSINPQNDD238 pKa = 3.1APQADD243 pKa = 4.2AQTITLDD250 pKa = 3.26EE251 pKa = 4.45DD252 pKa = 4.33TNTGITLTATDD263 pKa = 3.94IDD265 pKa = 4.46GDD267 pKa = 4.15SLSYY271 pKa = 10.8EE272 pKa = 3.96LVSQPEE278 pKa = 4.16NGTLTGNAPEE288 pKa = 4.6LVYY291 pKa = 10.68TPAPDD296 pKa = 4.88FFGQDD301 pKa = 2.7EE302 pKa = 4.67FTFRR306 pKa = 11.84ANDD309 pKa = 3.7GTEE312 pKa = 3.87DD313 pKa = 3.69SEE315 pKa = 4.73LAVISITVEE324 pKa = 3.75DD325 pKa = 4.06VLDD328 pKa = 4.1NKK330 pKa = 10.96APLAVNDD337 pKa = 3.8AFEE340 pKa = 4.12VLKK343 pKa = 11.21NSTANRR349 pKa = 11.84LDD351 pKa = 3.36VLLNDD356 pKa = 4.82SDD358 pKa = 4.54PDD360 pKa = 3.76GDD362 pKa = 4.48KK363 pKa = 9.86ITVVQVYY370 pKa = 10.54ANLGEE375 pKa = 4.28VSVTTDD381 pKa = 3.01GAVVYY386 pKa = 10.9SPMTDD391 pKa = 2.76STQNDD396 pKa = 3.85LLTYY400 pKa = 10.3VIEE403 pKa = 4.68DD404 pKa = 3.67EE405 pKa = 4.61FGEE408 pKa = 4.4SASAEE413 pKa = 3.89VHH415 pKa = 5.02ITVVTEE421 pKa = 4.38DD422 pKa = 4.2NLPPIAVDD430 pKa = 4.89DD431 pKa = 4.37SVTMQTGEE439 pKa = 4.38TITLDD444 pKa = 3.57VLANDD449 pKa = 4.59YY450 pKa = 11.21DD451 pKa = 4.35PEE453 pKa = 6.49DD454 pKa = 3.9DD455 pKa = 5.28AIEE458 pKa = 4.04LVAVSGDD465 pKa = 3.28VGEE468 pKa = 4.28VVIVDD473 pKa = 4.04GALQFTPQSNIHH485 pKa = 6.33GDD487 pKa = 3.6YY488 pKa = 10.77LITYY492 pKa = 7.36SIRR495 pKa = 11.84DD496 pKa = 3.37AAGNFASAEE505 pKa = 3.98VLVNVEE511 pKa = 4.34SEE513 pKa = 4.17SGPFITLPEE522 pKa = 4.49DD523 pKa = 3.36LCSEE527 pKa = 4.32LTVMANALYY536 pKa = 9.99TKK538 pKa = 10.45VDD540 pKa = 4.04LGQASAVDD548 pKa = 3.35RR549 pKa = 11.84FGNVLPVSLLDD560 pKa = 4.33GNTLYY565 pKa = 10.54PPGINQAFWSATDD578 pKa = 3.71AEE580 pKa = 5.13GNTAIATQLVCVAPLVSIQKK600 pKa = 10.0DD601 pKa = 3.32QTVLKK606 pKa = 10.44GSSTTIGVYY615 pKa = 10.74LNGEE619 pKa = 4.26SPVYY623 pKa = 9.85PLVVPYY629 pKa = 9.48TLSGDD634 pKa = 3.64ATEE637 pKa = 4.71EE638 pKa = 4.03DD639 pKa = 4.28HH640 pKa = 7.48NLEE643 pKa = 4.51AGDD646 pKa = 4.93LIIEE650 pKa = 4.66SGTTAYY656 pKa = 9.69IALDD660 pKa = 3.49TFINEE665 pKa = 4.27SNRR668 pKa = 11.84SDD670 pKa = 3.37RR671 pKa = 11.84VVTVSLDD678 pKa = 3.19EE679 pKa = 4.2SLNRR683 pKa = 11.84GAKK686 pKa = 9.89DD687 pKa = 3.2EE688 pKa = 4.76HH689 pKa = 6.41NVLLTEE695 pKa = 4.44RR696 pKa = 11.84NIEE699 pKa = 4.02PDD701 pKa = 2.85VTLQVLQNHH710 pKa = 6.58IKK712 pKa = 10.4RR713 pKa = 11.84LTVSRR718 pKa = 11.84RR719 pKa = 11.84GGAISVISMVEE730 pKa = 4.05DD731 pKa = 3.89PNLTDD736 pKa = 2.97SHH738 pKa = 6.98QYY740 pKa = 10.23AWITSDD746 pKa = 2.87HH747 pKa = 6.44RR748 pKa = 11.84LVNEE752 pKa = 4.19ATNDD756 pKa = 3.28TAFIFDD762 pKa = 4.31PSLLEE767 pKa = 3.76AGIYY771 pKa = 9.36HH772 pKa = 6.19VSIEE776 pKa = 4.38VTDD779 pKa = 3.63SGAPQLSDD787 pKa = 3.48SEE789 pKa = 4.58TIYY792 pKa = 10.69IEE794 pKa = 4.18VVEE797 pKa = 4.54EE798 pKa = 3.99LLSFDD803 pKa = 4.58DD804 pKa = 5.39HH805 pKa = 8.93SLDD808 pKa = 4.36SDD810 pKa = 3.79GDD812 pKa = 4.31LIPDD816 pKa = 3.68HH817 pKa = 6.56MEE819 pKa = 4.85GYY821 pKa = 10.42QDD823 pKa = 3.56TDD825 pKa = 3.08GDD827 pKa = 4.59GIPDD831 pKa = 3.55FLDD834 pKa = 5.67RR835 pKa = 11.84IDD837 pKa = 3.84EE838 pKa = 4.49CNVLQEE844 pKa = 4.13VAKK847 pKa = 10.9VHH849 pKa = 6.77DD850 pKa = 4.36AFLIEE855 pKa = 4.34GQAGVCLRR863 pKa = 11.84RR864 pKa = 11.84GDD866 pKa = 3.76HH867 pKa = 6.77SIGGEE872 pKa = 3.92TGGAQITNDD881 pKa = 5.07DD882 pKa = 3.76IAQDD886 pKa = 3.8EE887 pKa = 4.5LDD889 pKa = 4.51EE890 pKa = 5.64LVDD893 pKa = 5.54DD894 pKa = 5.03PDD896 pKa = 5.75AINVGGIFDD905 pKa = 4.33YY906 pKa = 10.55IAYY909 pKa = 9.81GMPEE913 pKa = 4.03QGTQFAIVMPQRR925 pKa = 11.84KK926 pKa = 8.56PIPADD931 pKa = 2.89AVYY934 pKa = 10.59RR935 pKa = 11.84KK936 pKa = 9.05FRR938 pKa = 11.84PGSGWGFFIEE948 pKa = 5.17DD949 pKa = 4.22ANNSLWSTPGDD960 pKa = 3.4VGFCPPPDD968 pKa = 3.69VNSGNSVWTPGLTEE982 pKa = 4.09GHH984 pKa = 6.56WCVQQIIEE992 pKa = 4.66DD993 pKa = 3.92GGVNDD998 pKa = 6.0DD999 pKa = 4.58DD1000 pKa = 4.71NLVNGTIVDD1009 pKa = 3.85PGGVGVMITSNALPEE1024 pKa = 4.47ASDD1027 pKa = 4.05DD1028 pKa = 4.2SVTLVVNQEE1037 pKa = 3.59ITVDD1041 pKa = 4.03VIANDD1046 pKa = 3.6TDD1048 pKa = 3.81QNGDD1052 pKa = 3.79PLLLTSATTTIGEE1065 pKa = 4.09VSIVDD1070 pKa = 3.6NAIHH1074 pKa = 5.96YY1075 pKa = 8.3VAALDD1080 pKa = 3.84YY1081 pKa = 11.12VGEE1084 pKa = 3.95ITINYY1089 pKa = 8.35GVTDD1093 pKa = 3.94SNGGTDD1099 pKa = 3.77HH1100 pKa = 7.47AVLTINLIDD1109 pKa = 3.93NKK1111 pKa = 10.91APLITNDD1118 pKa = 3.45SSEE1121 pKa = 4.06ILQGEE1126 pKa = 4.43TATLNLLTNDD1136 pKa = 4.13SDD1138 pKa = 4.41PDD1140 pKa = 3.88GDD1142 pKa = 3.87QMRR1145 pKa = 11.84LLSVEE1150 pKa = 3.86HH1151 pKa = 6.56SGVSFSEE1158 pKa = 3.84NGEE1161 pKa = 4.42VVFTPQSDD1169 pKa = 4.17FYY1171 pKa = 10.95GVLVIDD1177 pKa = 4.16YY1178 pKa = 9.42QVSDD1182 pKa = 2.95EE1183 pKa = 4.5WGNQSFGQWSVTVTEE1198 pKa = 3.83YY1199 pKa = 11.03HH1200 pKa = 6.92RR1201 pKa = 11.84IEE1203 pKa = 4.23ASTRR1207 pKa = 11.84GGGSLYY1213 pKa = 11.0GLLLLGVFVSVIRR1226 pKa = 11.84KK1227 pKa = 8.61VNWKK1231 pKa = 10.1ANRR1234 pKa = 11.84GGII1237 pKa = 3.85

Molecular weight: 131.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8M6J8|A0A3D8M6J8_9ALTE ThuA domain-containing protein OS=Alteromonas aestuariivivens OX=1938339 GN=DXV75_11660 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3306

0

3306

1141386

28

1682

345.2

38.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.099 ± 0.045

1.021 ± 0.015

5.574 ± 0.037

6.12 ± 0.039

4.137 ± 0.025

7.09 ± 0.037

2.319 ± 0.022

5.624 ± 0.031

4.376 ± 0.034

10.382 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.018

4.051 ± 0.031

4.298 ± 0.027

4.875 ± 0.038

5.186 ± 0.028

6.542 ± 0.03

5.19 ± 0.027

7.089 ± 0.037

1.362 ± 0.015

3.157 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski